Yazar "Aksungur, Zeynep" seçeneğine göre listele
Listeleniyor 1 - 20 / 21
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe Arginine, symmetric and asymmetric dimethylarginine levels in the molsidomine treatment of experimental ischemia-reperfusion retinopathy(2019) Polat, Nihat; Atabey Ozer, Murat; Parlakpinar, Hakan; Aksungur, Zeynep; Ozhan, Onural; Turkoz, YusufAbstract: This study aimed to evaluate the mean changes in Arginine, Asymmetric Dimethylarginine (ADMA) and Symmetric Dimethylarginine (SDMA) levels in the ischemia/reperfusion (I/R) retinopathy and efficacy of treatment with molsidomine by these levels. Experiments were performed on the New Zealand white rabbits each weighing approximately 2.5 kg. 28 rabbits were assigned to the following 4 groups, group 1 consisted sham, group 2 consisted I/R, group 3 consisted I/R+ treatment with molsidomine, group 4 consisted prophylaxis with molsidomine +I/R. In the group 2, 3 and 4, ischemia was induced by raising the intraocular pressure to 150 mmHg for 60 minutes. After 60 min, the IOP was returned to normal pressure. 4 mg/kg/day molsidomine was administered intraperitoneally four days after I/R in group 3, one day before I/R and three days after I/R in group 4. Arginine, ADMA and SDMA levels were measured on the aqueous humor. The mean arginine levels were 12.3±4.8 ?mol/L in group 1, 12.4±1.4 ?mol/L in group 2, 13.2±2.4 ?mol/L in group 3 and 13.7±4.3 ?mol/L in group 4. No difference was present between the groups (p=0.807). The mean ADMA levels were 2.6±0.8 ?mol/L, 7.3±2.7 ?mol/L, 0.5±0.5 ?mol/L and 2.5±1.0 ?mol/L respectively. Significant increase was present in the group 2 and significant decrease was present in the group 3 (p=0.001). The mean SDMA levels were 1.0±0.3 ?mol/L, 1.8±0.2 ?mol/L, 0.3±0.3 ?mol/L and 1.0±0.4 ?mol/L respectively. Significant increase was present in the group 2 and significant decrease was present in the group 3 (p=0.001). L-Arginine levels were kept steady, ADMA and SDMA values decreased with molsidomine. Four days treatment with molsidomine after I/R may be beneficial more than prophylaxis and three days treatmentÖğe Biochemical changes induced by grape seed extract and low level laser therapy administration during intraoral wound healing in rat liver: an experimental and in silico study(Taylor & Francis Inc, 2018) Erdemli, Mehmet Erman; Salmas, Ramin Ekhteiari; Durdagi, Serdar; Akgul, Hasan; Demirkol, Mehmet; Aksungur, Zeynep; Selamoglu, ZelihaIn the present study, the changes that occur in rat liver tissue as a result of the use of grape seed extract (GSE) and low level laser therapy (LLLT) in intraoral wound (IW) healing are analyzed using biochemical parameters. Diode laser application groups received 8J/cm(2) dose LLLT once a day for 4days (810nm wavelength, continuous mode, 0.25W, 9s). As a result of the biological parameter analysis, it was determined that the oxidative damage caused by the IWs and recovery period on 7th and 14th days could be substantially removed with GSE applications that have antioxidant capacity especially in rat liver tissue. In addition, the active compound of grape seed, catechin is studied in the active site of glycogen synthase kinase 3 (GSK3) target using molecular modeling approaches. Post-processing molecular dynamics (MD) results for catechin is compared with a standard GSK3 inhibitor. MD simulations assisted for better understanding of inhibition mechanism and the crucial amino acids contributing in the ligand binding. These results along with a through free energy analysis of ligands using sophisticated simulations methods are quite striking and it suggests a greater future role for simulation in deciphering complex patterns of molecular mechanism in combination with methods for understanding drug-receptor interactions.Öğe Biochemical investigation of the toxic effects of acrylamide administration during pregnancy on the liver of mother and fetus and the protective role of vitamin E(Taylor & Francis Ltd, 2017) Erdemli, Mehmet Erman; Altinoz, Eyup; Aksungur, Zeynep; Turkoz, Yusuf; Dogan, Zumrut; Bag, Harika GozukaraObjectives: To investigate the toxic effects occurring in the liver tissues of the pregnant rats and the fetuses, which are administered acrylamide and vitamin E as a protector during pregnancy. Materials and methods: This research was conducted with the permission of Laboratory Animals Ethical Board of Inonu University Faculty of Medicine. Forty rats, of which their pregnancy is validated via vaginal smear, were distributed into five different groups. On the 20th day of pregnancy, pregnant rats and fetuses are decapitated. Malondialdehyde (MDA), reduced glutathione (GSH), total antioxidant status (TAS), total oxidant status (TOS) and xanthine oxidase (XO) levels were measured in the liver samples taken from mother and fetuses. Results: It was detected that acrylamide administered during pregnancy increased MDA, TOS, XO levels statistically significantly and decreased the GSH level (p <= 0.05) in the pregnant rat liver tissue when compared to all other groups. In the vitamin E administered group; GSH, TAS levels significantly increased statistically and TOS and XO levels dropped to levels of the control group (p <= 0.05), in comparison to all other groups. Among all groups, no biochemical changes were observed in the fetus liver tissue (p > 0.05). Conclusion: The liver of pregnant rats functions as a protective pre-filter by detoxifying acrylamide effectively and the acrylamide that reaches fetus liver is detoxified by the cytochrome P-450 system of the fetus liver. To be able to figure out the biochemical mechanism, more advanced studies are needed.Öğe Can crocin play a preventive role in Wistar rats with carbon tetrachloride-induced nephrotoxicity?(Mashhad Univ Med Sciences, 2018) Erdemli, Mehmet Erman; Gul, Mehmet; Altinoz, Eyup; Aksungur, Zeynep; Gul, Semir; Bag, Harika GozukaraObjective(s): To investigate protective role of crocin by attempting to create nephrotoxicity with carbon tetrachloride. Materials and Methods: Ethics committee approval was obtained and 50 male Wistar rats were randomly divided into 5 groups that included 10 rats each: Control, Corn oil, Crocin, Carbon tetrachloride (CCl4), and Crocin + Carbon tetrachloride. Following the experiments, the rats were decapitated under anesthesia and incised kidney tissues were subjected to biochemical and histological examinations. Results: In the CCl4 administered group, MDA, TOS, Bun, and creatinine levels increased, GSH, SOD, CAT, and TAS levels decreased (P <= 0.05), glomerular collapse in kidney sections, narrowing and local occlusion in Bowman's space in certain glomeruli, inflammatory cell infiltration and congestion were observed when compared to all other groups. There was a significant decrease in increased MDA, TOS, Bun, and creatinine levels, and a significant increase in decreased GSH, SOD, CAT, and TAS levels in CCl4 + crocin administered group compared to the CCl4 group (P <= 0.05), local minimal glomerular damage, tubular damage, inflammatory infiltration, and vascular collagen symptoms were observed in kidney sections, however significant improvement was observed in damage findings when compared to the CCl4 group. Conclusion: At this dose and time interval, against a highly toxic chemical such as CCl4, crocin was able to suppress oxidative stress by playing a protective role in the kidney tissue.Öğe Crocin protects intestine tissue against carbon tetrachloride-mediated oxidative stress in rats(Aepress Sro, 2018) Cosgun, Binnaz E.; Erdemli, Mehmet E.; Gul, Mehmet; Gul, Semir; Bag, Harika G.; Aksungur, Zeynep; Altinoz, EyupSaffron is used in traditional medicine for its hypolipidemic, anti-inflammatory and anti carcinogenic properties as a natural remedy in treatment of diseases. The objective of the present study was to demonstrate the protective effect of crocin (one of the main ingredients of saffron) on carbon tetrachloride (CCl4) damage in intestinal mucosa. MDA, GSH, SOD, CAT, TAS and TOS levels were measured in experimental animal tissue samples and these were compared with histologic lesions induced by CCl4.CCl4 caused an increase in MDA, SOD, CAT and TOS levels and a significant decrease in GSH and TAS levels in rat intestinal tracts. After crocin treatment, serious improvements were observed in histological lesions and biochemical results in the intestinal tract. In conclusion, crocin inhibited the toxic effects induced by CCl4 in the intestine by its strong antioxidant properties.Öğe Does apocynin increase liver regeneration in the partial hepatectomy model?(Tubitak Scientific & Technological Research Council Turkey, 2023) Bilgic, Yilmaz; Kanat, Burhan Hakan; Ozhan, Onural; Yildiz, Azibe; Aksungur, Zeynep; Erdemli, Mehmet Erman; Vardi, NigarBackground/aim: Hepayocyte loss may develop secondary to liver surgery and at this point liver regeneration plays a significant act in terms of liver reserve. The purpose of this research was to investigate the efficacy of apocynin on liver regeneration and preservation after partial hepatectomy in rats.Materials and methods: A total of 32 rats, have been divided into 4 groups (n: 8) for hepatectomy model. Inflammatory and antiinflammatory parameters were measured from blood and liver tissue samples. In addition, the effects of apocynin were examined immunohistochemically and histopathologically from liver tissue. Results: In liver tissue samples, a significant difference has been found in glutathione peroxidase, total nitrite, catalase, oxidative stress index, total antioxidant and total oxidant status between sham and hepatectomy groups. A significant difference has been achieved between hepatectomy and posthepatectomy-Apocynin in terms of glutathione peroxidase and oxidative stress index. Total antioxidant status, oxidative stress index, and total oxidant status were significantly different only between the sham and the hepatectomy groups. Statistical differences were found between sham and hepatectomy groups and between hepatectomy and pre+post-hepatectomy-Apocynin groups in terms of serum glutathione, malondialdehyde, total nitrite, and L-Arginine. There were significant differences between the sham and hepatectomy groups, between hepatectomy and posthepatectomy-apocynin groups, between posthepatctomy-apocynin and pre+posthepatectomy-apocynin groups in terms of sinusoidal dilatation, intracytoplasmic vacuolization and glycogen loss (p < 0.001), in all histopathologic parameters except sinusoidal dilatation (p < 0.05). However, significant Ki-67 increases have been elaborated in hepatectomy, posthepatectomy-apocynin, and pre+posthepatectomy-apocynin groups compared to sham group (p < 0.001), in pre+posthepatectomy apocynin group compared to hepatectomy and posthepatectomy-apocynin groups (p < 0.001). Conclusion: Histopathology, immunohistochemistry, and biochemistry results of this study revealed that apocynin has a protective effect on enhancing liver regeneration in partial hepatectomy cases in rats.Öğe The effects of acrylamide and vitamin E on kidneys in pregnancy: an experimental study(Taylor & Francis Ltd, 2019) Erdemli, Mehmet Erman; Aksungur, Zeynep; Gul, Mehmet; Yigitcan, Birgul; Bag, Harika Gozukara; Altinoz, Eyup; Turkoz, YusufObjectives: The objective of this study is to investigate possible damages to kidney tissues of pregnant rats and their fetuses exposed to acrylamide during pregnancy and possible protective effects of vitamin E against these damages. Material and methods: Rats were randomly assigned to five groups of control, corn oil, vitamin E, acrylamide, vitamin E + acrylamide, six pregnant rats in each. Mother and fetal kidney tissues were examined for malondialdehyde (MDA), reductase glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), total antioxidant status (TAS), total oxidant status (TOS), urea, creatine, trace elements such as Zn and Cu in the serum and histopathological analyses were conducted. Results: It was determined that acrylamide, administered during pregnancy, statistically significantly increased MDA and TOS levels, maternal serum urea, creatinine, and Zn levels, while it decreased GSH, TAS, SOD, and CAT levels (p <= .05) when compared with all other groups in the kidney tissues of pregnant rats and their fetuses and caused tubular degeneration, hemorrhage, narrowing, and closure in Bowman's space, and, in the E vitamin group, it statistically significantly increased GSH, TAS, SOD, CAT, urea, creatinine, and Zn levels when compared with other groups and lowered TOS and MDA levels to those of the control group (p < .05) and there were no differences between the groups histologically. Conclusion: It was observed that acrylamide administered during pregnancy caused oxidative stress in kidney tissues of mother rats and their fetuses, resulting in tissue damage, and vitamin E application, which is considered to be a powerful antioxidant, inhibited oxidative stress.Öğe The effects of acrylamide and vitamin E on kidneys in pregnancy: An experimental study(Taylor and Francis Ltd, 2018) Aksungur, Zeynep; Gül, Mehmet; Yiğitcan, Birgül; Çanta, Harika Gozukara; Altınöz, E.; Türköz, YusufObjectives: The objective of this study is to investigate possible damages to kidney tissues of pregnant rats and their fetuses exposed to acrylamide during pregnancy and possible protective effects of vitamin E against these damages. Material and methods: Rats were randomly assigned to five groups of control, corn oil, vitamin E, acrylamide, vitamin E + acrylamide, six pregnant rats in each. Mother and fetal kidney tissues were examined for malondialdehyde (MDA), reductase glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), total antioxidant status (TAS), total oxidant status (TOS), urea, creatine, trace elements such as Zn and Cu in the serum and histopathological analyses were conducted. Results: It was determined that acrylamide, administered during pregnancy, statistically significantly increased MDA and TOS levels, maternal serum urea, creatinine, and Zn levels, while it decreased GSH, TAS, SOD, and CAT levels (p ≤ .05) when compared with all other groups in the kidney tissues of pregnant rats and their fetuses and caused tubular degeneration, hemorrhage, narrowing, and closure in Bowman’s space, and, in the E vitamin group, it statistically significantly increased GSH, TAS, SOD, CAT, urea, creatinine, and Zn levels when compared with other groups and lowered TOS and MDA levels to those of the control group (p < .05) and there were no differences between the groups histologically. Conclusion: It was observed that acrylamide administered during pregnancy caused oxidative stress in kidney tissues of mother rats and their fetuses, resulting in tissue damage, and vitamin E application, which is considered to be a powerful antioxidant, inhibited oxidative stress.Öğe Effects of apocynin on sciatic nerve injury in rabbits(Taylor & Francis Ltd, 2023) Durak, Mehmet Akif; Ozhan, Onural; Tetik, Bora; Yildiz, Azibe; Aksungur, Zeynep; Vardi, Nigar; Turkoz, YusufWe investigated the effects of apocynin (APO) on experimental sciatic nerve compression injury in rabbits. We used 21 male rabbits divided randomly into three groups of seven. The control group was subjected to sciatic nerve compression with no further intervention. The APO treated group was subjected to compression injury and 20 mg/kg APO was administered daily for 21 days by intraperitoneal injection beginning the day after the injury. The sham group was treated with APO without injury. The control group exhibited shrinkage of axons, disruption of myelin sheaths and loss of nerve fibers. The damage for the control group was significantly greater than for the sham group. The severity of histopathology was decreased in the APO treated group compared to the control group, as was the oxidative stress index. Our findings suggest that APO treatment may contribute to healing of sciatic nerve damage.Öğe The effects of grapeseed extract and low level laser therapy administration on the liver in experimentally fractured mandible(2017) Ege, Bilal; Aksungur, Zeynep; Erdemli, Mehmet Erman; Akgül, Hasan; Bağ, Harika Gözde Gözükara; Selamoğlu, ZelihaAim: The present study investigated the changes in the liver tissues of rats with experimentally fractured mandible following the use of Grape Extract (GSE) and Low Level Laser Therapy (LLLT) in healing the fracture in dentistry. Materials and Methods: 60 adult male Wistar Albino rats were randomly assigned to 5 main groups (Control, Fractured Mandible (FM), FM + GSE, FM + LLLT, FM + LLLT + GSE), and then these groups were divided into two groups of 7 and 21 days (n = 6). A vertical fracture line passing through the molar teeth was formed in the right mandibles of all subjects except for these in the control group, and the fracture was internally fixed with a four-hole microplate and four micro-screws. Malondialdehyde (MDA), reduced glutathione (GSH), superoxide dismutase (SOD), catalase (CAT) activities were analyzed to determine the changes caused by GSE and LLLT administration in rat liver tissues in fractured mandibles. Results: It was determined that MDA and SOD levels in FM group and GSH and CAT activity levels in FM + GSE group and MDA levels in FM + LLLT group and GSH and CAT levels in FM + GSE + LLLT group increased statistically significantly to the control group on days 7 and 21. Conclusion: Biochemical parameters were investigated on the 7th and the 21st days, and it was determined that the oxidative damage caused by mandibular defects could be eliminated substantially in the rat liver especially with the administration of grapeseed that has antioxidant capacity.Öğe The effects of grapeseed extract and low level laser therapy administration on the liver in experimentally fractured mandible(2017) Erdemli, Mehmet Erman; Akgul, Hasan; Ege, Bilal; Aksungur, Zeynep; Gozukara Bag, Harika; Selamoglu, ZelihaAim: The present study investigated the changes in the liver tissues of rats with experimentally fractured mandible following the use of Grape Extract (GSE) and Low Level Laser Therapy (LLLT) in healing the fracture in dentistry. Materials and Methods: 60 adult male Wistar Albino rats were randomly assigned to 5 main groups (Control, Fractured Mandible (FM), FM + GSE, FM + LLLT, FM + LLLT + GSE), and then these groups were divided into two groups of 7 and 21 days (n = 6). A vertical fracture line passing through the molar teeth was formed in the right mandibles of all subjects except for these in the control group, and the fracture was internally fixed with a four-hole microplate and four micro-screws. Malondialdehyde (MDA), reduced glutathione (GSH), superoxide dismutase (SOD), catalase (CAT) activities were analyzed to determine the changes caused by GSE and LLLT administration in rat liver tissues in fractured mandibles. Results: It was determined that MDA and SOD levels in FM group and GSH and CAT activity levels in FM + GSE group and MDA levels in FM + LLLT group and GSH and CAT levels in FM + GSE + LLLT group increased statistically significantly to the control group on days 7 and 21. Conclusion: Biochemical parameters were investigated on the 7th and the 21st days, and it was determined that the oxidative damage caused by mandibular defects could be eliminated substantially in the rat liver especially with the administration of grapeseed that has antioxidant capacityÖğe The effects of grapeseed extract and low level laser therapy administration on the liver in experimentally fractured mandible(2017) Erdemli, Mehmet Erman; Selamoğlu, Zeliha; Akgül, Hasan; Ege, Bilal; Bağ, Harika Gözde Gözükara; Aksungur, ZeynepAim: The present study investigated the changes in the liver tissues of rats with experimentally fractured mandible following the use of Grape Extract (GSE) and Low Level Laser Therapy (LLLT) in healing the fracture in dentistry.Materials and Methods: 60 adult male Wistar Albino rats were randomly assigned to 5 main groups (Control, Fractured Mandible (FM), FM + GSE, FM + LLLT, FM + LLLT + GSE), and then these groups were divided into two groups of 7 and 21 days (n = 6). A vertical fracture line passing through the molar teeth was formed in the right mandibles of all subjects except for these in the control group, and the fracture was internally fixed with a four-hole microplate and four micro-screws. Malondialdehyde (MDA), reduced glutathione (GSH), superoxide dismutase (SOD), catalase (CAT) activities were analyzed to determine the changes caused by GSE and LLLT administration in rat liver tissues in fractured mandibles.Results: It was determined that MDA and SOD levels in FM group and GSH and CAT activity levels in FM + GSE group and MDA levels in FM + LLLT group and GSH and CAT levels in FM + GSE + LLLT group increased statistically significantly to the control group on days 7 and21.Conclusion: Biochemical parameters were investigated on the 7th and the 21st days, and it was determined that the oxidative damagecaused by mandibular defects could be eliminated substantially in the rat liver especially with the administration of grapeseed that has antioxidant capacity.Öğe The effects of total parenteral nutrition on telomerase expression in rabbit(2018) Gürünlüoğlu, Kubilay; Bayrakci, Ercan; Kocabıyık, Alper; Gökçe, Hasan; Taşkapan, Mehmet Çağatay; Taşcı, Aytaç; Aksungur, Zeynep; Arslan, Ahmet Kadir; Demircan, MehmetAbstract: Aim: Total parenteral nutrition (TPN) is a technique which is use to give vitally mandatory substances in to the venous compartments whenever the gastrointestinal system cannot be used by the patients. Telomerase catalyzes DNA synthesis, which is necessary to maintain telomere length and stabilize the genome to allow continued cell proliferation. In this study, we explored the effects of TPN administration on telomerase reverse transcriptase (TERT) expression in various tissue and serum telomerase level. Material and Methods: In this study a number of 42 same-aged albino, equal number of male and female, new zealand rabbits were use, divided in to three groups. Group 1 rabbits received TPN for 10 days via a central venous catheter. Group 2 received 50 mL/ kg/day physiological saline via a central venous catheter. Group 3 served as the control group. The rabbits were sacrificed after 10 days, and serum telomerase levels were measured by enzyme-linked immunosorbent assay. TERT expression in gonadal, liver, jejunum, and skin tissues were determined immunohistochemically. Blood samples were obtained before and after TPN and saline administration in the TPN and serum saline groups, respectively, and at the end of the experiment in the control group. Results: Telomerase expression in liver, gonads and serum level of TPN group was significantly higher than control and serum saline groups. Conclusion: TPN may be a positive effect in liver and gonadal telomer kinetic. However, we think that TPN increases DNA damage throughout the body.Öğe The effects of total parenteral nutrition on telomerase expression in rabbit(2018) Gurunluoglu, Kubilay; Bayrakci, Ercan; Gokce, Hasan; Kocbiyik, Alper; Taskapan, Cagatay; Tasci, Aytac; Aksungur, Zeynep; Arslan, Ahmet Kadir; Demircan, MehmetAbstract Aim: Total parenteral nutrition (TPN) is a technique which is use to give vitally mandatory substances in to the venous compartments whenever the gastrointestinal system cannot be used by the patients. Telomerase catalyzes DNA synthesis, which is necessary to maintain telomere length and stabilize the genome to allow continued cell proliferation. In this study, we explored the effects of TPN administration on telomerase reverse transcriptase (TERT) expression in various tissue and serum telomerase level. Material and Methods: In this study a number of 42 same-aged albino, equal number of male and female, new zealand rabbits were use, divided in to three groups. Group 1 rabbits received TPN for 10 days via a central venous catheter. Group 2 received 50 mL/ kg/day physiological saline via a central venous catheter. Group 3 served as the control group. The rabbits were sacrificed after 10 days, and serum telomerase levels were measured by enzyme-linked immunosorbent assay. TERT expression in gonadal, liver, jejunum, and skin tissues were determined immunohistochemically. Blood samples were obtained before and after TPN and saline administration in the TPN and serum saline groups, respectively, and at the end of the experiment in the control group. Results: Telomerase expression in liver, gonads and serum level of TPN group was significantly higher than control and serum saline groups. Conclusion: TPN may be a positive effect in liver and gonadal telomer kinetic. However, we think that TPN increases DNA damage throughout the body.Öğe Gebe Ratlara uygulanan Akrilamid ve E Vitaminin Plasenta Dokusu Üzerine Olası Etkilerinin Araştırılması(Gıda Bilimi ve Teknolojisi, 2017) Erdemli, Mehmet Erman; Altınöz, Eyüp; Aksungur, Zeynep; Doğan, Zümrüt; Gözükara, Harika Bağ; Türköz, Yusufz: Gebelik boyunca akrilamid (AA) ve koruyucu olarak E vitamini (E vit) uygulanan ratların plasenta dokularında meydana gelen değişimlerin incelenmesi amaçlanmıştır. Vajinal smear ile gebelikleri doğrulanan 30 rat; Kontrol, Mısır yağı, E vitamini, Akrilamid, E vitamini + Akrilamid: grupları olmak üzere rastgele seçilerek 5 farklı gruba ayrıldı. Gebeliğin 20. gününde gebe ratlar dekapite edildi. Plasenta dokularında; malondialdehid (MDA), redükte glutatyon (GSH), total antioksidan kapasite (TAS), total oksidan kapasite (TOS) ve ksantin oksidaz (XO) düzeyleri ölçüldü. Gebe ratların plasenta dokusunda: gebelik boyunca uygulanan akrilamidin; diğer tüm gruplarla kıyaslandığında MDA, TOS, XO seviyelerini istatistiki olarak anlamlı ölçüde arttırdığı, GSH ve TAS seviyesini ise azalttığı, E vitamini uygulanan grupta ise, diğer tüm gruplara kıyasla GSH, TAS seviyelerini istatistiki olarak anlamlı ölçüde arttığını ve TOS ile XO seviyelerini ise kontrol grubu seviyelerine düşürdüğü tespit edildi. Ağız yoluyla gebe ratlara verilen AA, plasenta dokusunda MDA, XO ve TOS seviyelerini arttırarak antioksidan/oksidan dengeyi oksidanlar lehine değiştirdiği ve oksidatif strese neden olduğu, E vitamini uygulamasının ise antioksidan/oksidan dengeyi normal sınırlara getirerek oksidatif stres kaynaklı toksikasyonları önlediği görülmektedir. Başlık (İngilizce): Research on Possible Effects of Acrylamide and Vitamin E Administered to Pregnant Rats on Placenta Tissue Öz (İngilizce): Investigate the changes that occur in the placenta tissues of pregnant rats that were administered acrylamide (AA) and vitamin E as a protective agent during pregnancy. Thirty rats that were proven positive for pregnancy with vaginal smear test were randomly distributed into control, corn oil, vitamin E, acrylamide and vitamin E + acrylamide groups. Pregnant rats were decapitated on the 20th day of the experiment. Malondialdehyde (MDA), reduced glutathione (GSH), total antioxidant capacity (TAS), total oxidant capacity (TOS) and Xanthine oxidase (XO) levels were measured in placenta tissues. It was determined that acrylamide application during pregnancy statistically significantly increased MDA, TOS and XO levels and reduced GSH and TAS levels in the placenta tissue of pregnant rats when compared to all other groups, and GAS and TAS levels statistically significantly increased in vitamin E administered group when compared to all other groups and TOS and XO levels were decreased to control group levels. It was observed that orally administered AA changed the antioxidant / oxidant equilibrium favoring the oxidants by increasing MDA, XO and TOS levels in pregnant rats and caused oxidative stress, while vitamin E administration returned the antioxidant / oxidant equilibrium back to normal levels, preventing oxidative stress induced toxicity.Öğe Hepatoprotective effects of crocin on biochemical and histopathological alterations following acrylamide-induced liver injury in Wistar rats(Elsevier France-Editions Scientifiques Medicales Elsevier, 2017) Gedik, Sema; Erdemli, Mehmet Erman; Gul, Mehmet; Yigitcan, Birgul; Bag, Harika Gozukara; Aksungur, Zeynep; Altinoz, EyupThe objective of the present study is the treatment of oxidative damage caused by acrylamide induced oxidative stress in rats with the administration of a strong antioxidant, namely crocin. High acrylamide (AA) levels have genotoxic, carcinogenic and neurotoxic effects on living organisms. In the present study, 40 Wistar rats were randomly divided into four equal groups. These groups were control, acrylamide (25 mg/kg), crocin (50 mg/kg), acrylamide + crocin (25 mg/kg acrylamide and 50 mg/kg crocin) groups. At the end of the application, biochemical and histological variations were examined in liver and blood samples. It was observed that acrylamide administration significantly decreased liver GSH and TAS levels when compared to the control group. On the contrary, it was also observed that AST, ALT, ALP, SOD and CAT activities and TOS and MDA levels increased as a result of acrylamide administration. Histopathological examinations demonstrated inflammatory cell infiltration, hepatocellular necrosis and hemorrhage areas in AA group liver sections. Furthermore, intracytoplasmic vacuolization was detected in hepatocytes. After crocin treatment, it was observed that GSH and TAS levels increased while AST, ALT, ALP, SOD and CAT activities and TOS and MDA levels decreased. Significant decreases were observed in inflammatory cell infiltration and vascular congestion in liver sections and intracytoplasmic vacuolization in hepatocytes after the crocin treatment, while no hepatocellular necrosis and hemorrhages were observed. In the present study, it was demonstrated that crocin treatment removed acrylamide induced liver damage due to the strong antioxidant properties of crocin.Öğe Investigation of wet cupping therapy's effect on oxidative stress based on biochemical parameters(Elsevier Science Inc, 2019) Ersoy, Suleyman; Altinoz, Eyup; Benli, Ali Ramazan; Erdemli, Mehmet Erman; Aksungur, Zeynep; Bag, Harika Gozukara; Engin, Velittin SelcukIntroduction: Wet cupping therapy is one of the main applications which has been used in the Unani medicine system. Current literature explaining how this traditional treatment method works is still limited. The aim of this study was to investigate whether wet cupping therapy could affect reactive oxygen species and antioxidant levels. Methods: This was a single arm pre and posttest intervention study conducted in Karabuk University Teaching and Research Hospital (KUTRH) between 1st January and 1st May 2018. Twenty-four participants were included. Wet Cupping Therapy was applied to all individuals once every month for three months. Venous blood samples were collected before the first (Venousl) and after the last application (Venous 2) and cupping blood samples were collected during the first and last applications. Malondialdehyde (MAD) and total oxidant status (TOS) which both indicate oxidation; glutathione (GSH), superoxide dismutase (SOD), total antioxidant status (TAS), catalase (CAT) activities that indicate antioxidant effect, and protein levels were measured on the obtained serums. Results: The highest MDA and TOS levels and the lowest TAS, GSH, SOD and CAT levels were found in initial cupping blood. Significant improvement was observed in second cupping blood for these parameters when compared to initial values. We also found that MDA and TOS levels, as well as TAS, GSH, SOD and CAT levels, all changed favorably in the Venous 2 blood sample compared to the Venous 1 (p < 0.001). Conclusion: Wet cupping therapy seems a promising method for increasing antioxidant levels and curbing oxidative stress.Öğe Protective effect of dexpanthenol against cisplatin-induced hepatotoxicity(Spandidos Publ Ltd, 2018) Bilgic, Yilmaz; Akbulut, Sami; Aksungur, Zeynep; Erdemli, Mehmet Erman; Ozhan, Onural; Parlakpinar, Hakan; Vardi, NigarThe aim of the present study was to investigate the protective effect of dexpanthenol (Dexp) against cisplatin (Cis)-induced hepatotoxicity. Thirty-two Sprague Dawley rats were divided into four groups: Control group (n=8), Dexp group (n=8, 500 mg/kg/ip/daily single dose/3 days Dexp), Cis group (n=8, 7 mg/kg/ip/single dose Cis) and Cis+Dexp group (n=8, 500 mg/kg/ip/daily single dose/3 days Dexp +7 mg/kg/ip/single dose Cis). MDA, CAT, GSH, GSH-Px, TOS, TAS, OSI, Total Nitrit, IL-1 beta, IL-6 and TNF-alpha levels were analyzed in liver tissue samples. After paraffinization of liver tissue samples, histopathological (congestion, loss of glycogen, number of Kupffer cells) and immunohistochemical (caspase-3 expression) parameters were assessed on the paraffinized liver sections. GSH, TAS, TOS, OSI, Tot Nit, L-Arginine, ADMA and SDMA levels were measured in the serum samples. Statistically significant differences were found between the groups in terms of all liver tissue biochemical parameters, with the exception of IL-1 beta and TNF-alpha levels. GSH, CAT, GSH-Px, TAS and Tot Nit levels were significantly higher in the Cis+Dexp group compared to the Cis group, whereas MDA, TOS, OSI and IL-6 levels were higher in the Cis group. Similarly, serum GSH, TAS, Tot Nit levels were higher in the Cis+Dexp group whereas TOS, L-Arginine, ADMA and SDMA levels were higher in Cis group. There were statistically significant differences between Control and Cis groups in terms of congestion increase, increase of glycogen loss, increase of Kupffer cell number and increase of caspase-3 expression (P<0.001). There was a statistically significant difference between the Cis and the Cis+Dexp groups in terms of histopathologic parameters, with the exception of congestion (P<0.001). To conclude, histopathological, immunohistochemical, and biochemical results of this study demonstrated that Dexp has a protective effect against Cis-induced hepatotoxicity.Öğe Protective Effects of Hypericum perforatum and Quercetin in a Rat Model of Ischemia/Reperfusion Injury of Testes(Georg Thieme Verlag Kg, 2018) Suzen, Alev; Tekin, Leyla; Erdemli, Mehmet Erman; Erturk, Nazile; Aksungur, Zeynep; Aktas, SerdarPurpose This study aimed to compare the protective effects of Hypericum perforatum (Hp) and quercetin, a flavonoid, against ischemia/reperfusion (I/R) injury in rat testes. Materials and Methods This study included 28 male Wistar albino rats that were divided into four groups. Except for the sham group, torsion was created by rotating both testes at an angle of 720 degrees clockwise for 2 hours. The Hp and quercetin groups received 25 mg/kg Hp and quercetin intraperitoneally 30 minutes before detorsion, respectively. Orchiectomy was performed for the measurement of markers of oxidative stress and histopathological examination. Results In the Hp and quercetin groups, malondialdehyde (MDA) and nitric oxide (NO) levels and total oxidant capacity were significantly lower, the glutathione level and total antioxidant status were significantly higher, and Johnsen's testis biopsy scores were significantly higher than in the torsion/detorsion group (p=0.001). The markers of oxidative injury were significantly lower (p=0.001) and total antioxidant status was significantly higher (p=0.001), except for glutathione (p=0.62) in the Hp group than in the quercetin group. Johnsen's score between Hp and quercetin groups was not significantly different (p=0.80). Conclusion Both Hp and quercetin have protective effects against I/R injury of the testes, but the protective effect of Hp was found to be stronger than that of quercetin.Öğe Protective effects of hypericum perforatum and quercetin in a rat model of ıschemia/reperfusionınjury of testes(GEORG THIEME VERLAG KG, RUDIGERSTR 14, D-70469 STUTTGART, GERMANY, 2018) Aksungur, ZeynepPurpose This study aimed to compare the protective effects of Hypericum perforatum (Hp) and quercetin, a flavonoid, against ischemia/reperfusion (I/R) injury in rat testes. Materials and Methods This study included 28 male Wistar albino rats that were divided into four groups. Except for the sham group, torsion was created by rotating both testes at an angle of 720 degrees clockwise for 2 hours. The Hp and quercetin groups received 25 mg/kg Hp and quercetin intraperitoneally 30 minutes before detorsion, respectively. Orchiectomy was performed for the measurement of markers of oxidative stress and histopathological examination. Results In the Hp and quercetin groups, malondialdehyde (MDA) and nitric oxide (NO) levels and total oxidant capacity were significantly lower, the glutathione level and total antioxidant status were significantly higher, and Johnsen's testis biopsy scores were significantly higher than in the torsion/detorsion group (p=0.001). The markers of oxidative injury were significantly lower (p=0.001) and total antioxidant status was significantly higher (p=0.001), except for glutathione (p=0.62) in the Hp group than in the quercetin group. Johnsen's score between Hp and quercetin groups was not significantly different (p=0.80). Conclusion Both Hp and quercetin have protective effects against I/R injury of the testes, but the protective effect of Hp was found to be stronger than that of quercetin.
