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    Investigating the possible protective effect of caffeic acid phenethyl ester on aquaporin-2 changes in renal ischemia-reperfusion injury in rats
    (Mexican Acad Surgery, 2025) Sapmaz, Hilal I.; Kose, Evren; Karaca, Zeynal M.; Akyurek, Sema; Parlakpinar, Hakan; Deresoy, Faik A.; Turkoz, Yusuf
    Objective: We aimed to investigate the changes in renal aquaporins (AQP) of rats in renal ischemia-reperfusion (I/R) injury and the protective effects of caffeic acid phenethyl ester (CAPE) against these changes. Methods: Forty-five adult rats were divided into six groups: control, sham (right nephrectomy), I/R (right nephrectomy + left kidney I/R), I/R+CAPE (I/R procedure after i.p. CAPE), sham + CAPE, and sham + dimethyl sulfoxide. Blood urea nitrogen, Cr, and K+ levels were measured in the sera. Tissues were stained with hematoxylin and eosin for histopathological examination. For immunohistochemical analysis, AQP2 was applied using the streptavidin/biotin/peroxidase system. AQP2 gene expression in kidney tissues was examined by polymerase chain reaction (PCR). Results: In the I/R, congestion, inflammation, and necrosis were found to increase compared to the control. In the I/R+CAPE, improvement was observed in necrosis compared to the I/R. There was a decrease in AQP2 expression in the I/R. In PCR, no significant difference was observed in AQP2 gene expression between the I/R and control and between the I/R+CAPE and I/R. Conclusion: Renal I/R inhibits the production of AQP2 in the kidney and causes histological and biochemical damage. CAPE administration before I/R has a protective effect on the kidney.