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    Does intraperitoneal medical ozone preconditioning and treatment ameliorate the methotrexate induced nephrotoxicity in rats?
    (E-Century Publishing Corp, 2015) Aslaner, Arif; Cakir, Tugrul; Celik, Betul; Dogan, Ugur; Mayir, Burhan; Akyuz, Cebrail; Polat, Cemal
    Methotrexate is a chemotherapeutic agent used for many cancer treatments. It leads to toxicity with its oxidative injury. The purpose of our study is investigating the medical ozone preconditioning and treatment has any effect on the methotrexate-induced kidneys by activating antioxidant enzymes in rats. Eighteen rats were divided into three equal groups; control, Mtx without and with medical ozone. Nephrotoxicity was performed with a single dose of 20 mg/kg Mtx intraperitoneally at the fifteenth day of experiment on groups 2 and 3. Medical ozone preconditioning was performed at a dose of 25 mcg/ml (5 ml) intraperitoneally everyday in the group 3 and treated with medical ozone for five more days while group 2 was received only 5 ml of saline everyday for twenty days. All rats were sacrificed at the end of third week and the blood and kidney tissue samples were obtained to measure the levels of TNF-alpha, IL-1 beta, malondialdehyde, glutathione and myeloperoxidase. Kidney injury score was evaluated histolopatologically. Medical ozone preconditioning and treatment ameliorated the biochemical parameters and kidney injury induced by Mtx. There was significant increase in tissue MDA, MPO activity, TNF-alpha and IL-1 beta (P<0.05) and significant decrease in tissue GSH and histopathology (P<0.05) after Mtx administration. The preconditioning and treatment with medical ozone ameliorated the nephrotoxicity induced by Mtx in rats by activating antioxidant enzymes and prevented renal tissue.
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    Effects of Saint John’s Wort extract on intestinal injury and apoptosis
    (2021) Gul, Mehmet; Akyuz, Cebrail; Sunamak, Oguzhan; Velioglu Ogunc, Ayliz; Sehirli, Ahmet Ozer
    Aim: Reactive oxygen species play an important role in the pathophysiology of intestinal ischemia/reperfusion (I/R) injury by causing apoptosis. The present study aimed at exploring the possible protective effect of Saint John’s Wort (SJW) extract in I/R injury. Materials and Methods: Twenty four healthy male Wistar rats of 6 to 8 weeks old weighting averagely 200 to 250 g were studied. They were fed on standard rat food and drinking water at room temperature with 12/12 hours periods of day and night. SJW (300 mg/kg, peroral) or saline was given by oral route for 3 days prior to ischemia, 30 minutes before the ischemia, and just before reperfusion. To the rats in the control group, sham operation and application of saline were done. Levels of TNF-α, IL-1β and LDH were measured in the serum samples. In the small bowel tissue, levels of malonaldehyde (MDA) and glutathione (GSH) and activity of myeloperoxidase (MPO) and Na-K ATPase and level of caspase-3/β-actine and Bcl-2/β-actine were measured. Results: I/R increased serum levels of LDH, TNF-α and IL-1β, small bowel level of MDA and MPO whereas it decreased level of GSH and activity of Na-K ATPase in the small bowel tissue. The level of caspase-3/β-actine increased while the level of Bcl-2/β-actine decreased in the I/R group compared to the controls. With the application of SJW, these values approached the control levels. Conclusion: These results indicate that SJW recovers small bowel function by reducing oxidation injury during I/R.