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    Comparison of Rotavirus Genotypes Before and During the COVID-19 Pandemic
    (Galenos Publ House, 2024) Alici, Ayse; Tanriverdi, Elif Seren; Budak, Ilayda; Akkan, Murat; Yenisehirli, Gulgun; Otlu, Baris
    Introduction: Rotaviruses are the leading cause of severe diarrhea in infants and young children worldwide. To date, 32 distinct G genotypes and 47 distinct P genotypes have been identified in group A rotaviruses. Following the Coronavirus disease-2019 (COVID-19) pandemic, our country implemented several measures that effectively reduced the incidence of infectious diseases, including acute gastroenteritis associated with COVID-19. In this study, we investigate whether the measures implemented following the COVID-19 pandemic led to changes in the rotavirus genotype distribution. Materials and Methods: A total of 128 stool samples that tested positive for rotavirus antigen- 64 from the pre-pandemic period and 64 from the pandemic period- were further analyzed for genotyping. As determined by reverse transcription-polymerase chain reaction, rotavirus RNA was detected in 50 (78%) samples from the pre-pandemic period and 51 (80%) samples from the pandemic period. Results: In the pre-pandemic period, the following results were observed among the patients studied by us: G9P[8] in 24 (48%), G1P[8] in 14 (28%), G2P[8] in five (10%), G2P[4] in three (6%), G3P[8] in two (4%), G4P[8] in one (2%), and G9P[4] in one (2%). During the pandemic period, the following results were observed in the patients studied by us: G9P[8] in 28 (54%), G1P[8] in 12 (24%), G2P[8] in six (12%), G2P[4] in two (4%), G3P[8] in one (2%), G4P[8] in one (2%), and G9P[4] in one (2%). Conclusion: In our study, G9P[8] was the dominant genotype during both periods, showing no significant difference in rotavirus genotypes between the pre-pandemic and pandemic periods.
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    Detection of SARS-CoV-2 RNA in the Serum Samples of Healthy Blood Donors
    (Galenos Publ House, 2023) Dalgic, Bahise Cagla Taskin; Yenisehirli, Gulgun; Tanriverdi, Elif Seren; Alici, Ayse; Otlu, Baris
    Introduction: The transmission rate of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) by blood transfusion is thought to be low; however, it has not yet been proven whether the virus is transmitted by blood transfusion. Published studies have reported that SARS-CoV-2 RNA has been detected in the blood, plasma, or serum of infected individuals. This study aimed to investigate the presence of SARS-CoV-2 RNA in the serum of blood donors and evaluate the risk of transmission of SARS-CoV-2 by transfusion. Materials and Methods: In this study, 200 blood samples were taken from volunteer blood donors. In all serum samples, SARS-CoV-2 RNA was detected by reverse-transcription quantitative polymerase chain reaction assay. Medical records of the donors and recipients were retrospectively reviewed. Results: SARS-CoV-2 RNA was detected in seven (3.5%) of the donor serum samples. None of the positive donors had symptoms of coronavirus disease 2019 (COVID-19), and none had been admitted to the hospital after donation. Seven SARS-CoV-2 RNA-positive donor blood components were given to 12 recipients. No medical records indicated that COVID-19 occurred after the transfusion of blood components for recipients. Conclusion: This study demonstrated the presence of SARS-CoV-2 RNA in the serum of asymptomatic donors. Although our data suggest that the transfusion of blood products from asymptomatic donors to recipients with SARS-CoV-2 RNA in their serum may not result in COVID-19, further studies are needed to prove that SARS-CoV-2 is not transmitted by blood transfusion.
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    Investigation of Clonal Relationship Between Candida Parapsilosis and Candida Glabrata Strains Isolated from Blood Culture by Pulse Field Gel Electrophoresis
    (Galenos Publ House, 2025) Alici, Ayse; Tanriverdi, Elif Seren; Yenisehirli, Gulgun; Otlu, Baris
    Aim: Candidemia may occur when endogenous Candida species in the intestinal microbiota enter the bloodstream in patients with risk factors. Apart from endogenous transmission, patients can also transmit it exogenously. In the current study, we aimed to investigate the fluconazole susceptibility and genotypes of Candida parapsilosis (C.parapsilosis) and Candida glabrata (C. glabrata) strains that cause candidemia. Methods: Twenty-six C. parapsilosis and sixteen C. glabrata strains were included in the study. Fluconazole sensitivity was determined by the broth microdilution method. Genotyping of strains was done by pulsed-field gel electrophoresis. Results: Nine (34%) of C. parapsilosis strains and 3 (19%) of C. glabrata strains were found to be resistant to fluconazole. Among 26 C. parapsilosis isolates, 17 different genotypes were detected and clustered isolates were collected in five clusters. Fourteen out of the 26 C. parapsilosis isolates are placed in one of the clusters, with a clustering rate of 53.8%. Among 16 C. glabrata isolates, 11 different genotypes were detected and divided into four clusters. Nine out of the 16 C. glabrata isolates are placed in one of the clusters, with a clustering rate of 56.2%. Conclusion: Our data indicated the possibility of nosocomial transmission of C. parapsilosis and C. glabrata among intensive care unit patients in our hospital. Infection control policies should be strictly applied in our hospital to prevent cross-transmission.