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    Differential expression of inflammasome regulatory transcripts in periodontal disease
    (Wiley, 2020) Aral, Kubra; Berdeli, Eynar; Cooper, Paul Roy; Milward, Michael Robert; Kapila, Yvonne; Unal, Beyza Karadede; Aral, Cuneyt Asim
    Background The inflammasome modulates the release of key proinflammatory cytokines associated with periodontal disease pathogenesis. The aim of this study was to evaluate the expression of proteins that regulate the inflammasome, namely pyrin domain-only proteins (POPs), caspase activation recruitment domain (CARD)-only proteins, and tripartite motif-containing (TRIM) proteins, in periodontal diseases. Methods A total of 68 participants (34 males and 34 females) were divided into four groups, including periodontal health (H), gingivitis (G), chronic periodontitis (CP), and aggressive periodontitis (AgP) based on clinical parameters. Gingival tissue samples were obtained from all participants for reverse transcription polymerase chain reaction (RT-PCR)-based gene expression analyses of molecules that regulate the inflammasome, including apoptosis-associated speck-like protein (ASC) containing CARD, caspase-1, interleukin-1 beta (IL-1 beta), interleukin-18 (IL-18), nucleotide-binding domain, leucine rich family (NLR) pyrin domain containing 3 (NLRP3), NLR family pyrin domain containing 2 (NLRP2), AIM2 (absent in melanoma 2), POP1, POP2, CARD16, CARD18, TRIM16, and TRIM20 by RT-PCR. Results NLRP3 and IL-1 beta were upregulated in the G, CP, and AgP groups compared with group H (P < 0.05). AIM2 was downregulated in the CP group compared with the H, G, and AgP groups (P < 0.05). TRIM20, TRIM16, and CARD18 were downregulated in the G, CP, and AgP groups compared with the H group (P < 0.05). POP1 and POP2 were downregulated in the CP and AgP, and AgP and G groups, respectively (P < 0.05). Conclusion Active periodontal disease may result in downregulation of inflammasome regulators that may increase the activity of NLRP3 and IL-1 beta in periodontal disease.
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    Oxidative stress, neutrophil elastase and IGFBP7 levels in patients with oropharyngeal cancer and chronic periodontitis
    (Wiley, 2020) Aral, Cuneyt Asim; Olcer, Sude Nur; Aral, Kubra; Kapila, Yvonne
    Objective The present study focused on investigating levels of oxidative stress, neutrophil elastase (NE), and insulin-like growth factor-binding protein 7 (IGFBP7) in oropharyngeal cancers (OC) with the presence and absence of periodontitis. Materials and Methods A healthy non-periodontitis group (H-NP; n = 20), a systemically healthy chronic periodontitis group (H-P; n = 20), a non-periodontitis group with OC (OC-NP; n = 12), and a chronic periodontitis group with OC (OC-P; n = 16) formed the study groups. The levels of NE and IGFBP7 were measured in gingival crevicular fluid (GCF) and saliva. In addition, oxidative status was determined by evaluating total oxidant status (TOS), total antioxidant status (TAS), and OSI (TOS/TAS). Results Gingival crevicular fluid NE was higher in all the groups compared with the H-NP group (p < .01). Salivary NE was higher in the OC-P and H-P groups compared with the H-NP and OC-NP groups (p < .05). Salivary IGFBP7 was significantly higher in the OC-NP and OC-P groups compared with the H-NP and H-P groups (p < .001). GCF TOS and OSI levels were significantly higher in all groups compared with the H-NP group (p < .05). Conclusions Gingival crevicular fluid NE levels were lower in healthy conditions compared with periodontal disease and OC. Salivary NE levels were higher in periodontal disease compared to states with no periodontal disease. Salivary IGFBP7 levels were higher in OC. Further analyses may help determine whether high salivary IGFBP7 levels distinguish OC from healthy conditions.