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    Genotyping of Enterococcus casseliflavus strains isolated from various water samples
    (Elsevier Sci Ltd, 2011) Korkoca, Hanifi; Bayram, Banu; Atalan, Ekrem; Bayram, Yasemin; Onlu, Harun; Bozari, Sedat
    [Abstract Not Available]
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    In vitro callus propagation and antibacterial activities of callus an edible endemic and medicinal plant Scutellaria orientalis L. subsp bicolor
    (Mattioli 1885, 2016) Ozdemir, Fethi Ahmet; Kilic, Omer; Atalan, Ekrem
    Plants are a stupendous source for exploration of new medicinal products for drug development. Today several drugs used to treat many diseases are simply synthetic modifications or copies of naturally obtained plant substances or extracts. Medicinal plants offer best source to obtain a variety of new drugs; and new plants need to be investigated for their potential use against various microorganisms including bacteria. Scutellaria orientalis subsp. bicolor in the Lamiaceae (mint) family, is an endemic plant species that grows widely on abondoned lands at altitude of 1000-2800 meters in Eastern Anatolia. The plant or extracts are popularly used in preparation of various traditional medicines to treat many diseases including stress, breast and ovarian cancers; however there is need to establish validity of these methodologies scientifically as plant tissue culture techniques could serve as an alternative way to produce them. This study aimed to documents crude callus extracts from hypocotyl explants obtained from 17 days old plantlets of Scutellaria orientalis subsp. bicolor for antibacterial activities. Research findings clearly showed inhibition of growth of Staphylococcus aureus COWAN 1 and Bacillus subtilis var. niger ATCC 10 in the extracts determined by disc diffusion methodology. Antibacterial activity was evaluated by measuring zone diameters in mm. No antibacterial activities were detected in control group. The hexane, acetone and methanol extracts of S. orientalis subsp. bicolor calli exhibited inhibitory action against both bacterial strains. Moreover, callus extracts showed less anti bacterial activities on calli developed on MS medium containing NAA compared to calli obtained on MS medium containing BAP. Furthermore, the results confirmed inhibitory effects of increased BAP concentrations on callus weight, size and quantity of extracts. Moreover, methanol extracts showed more antibacterial activity compared to acetone and hexane extracts. Better knowledge about antibacterial activities of this endemic plant sub species could be highly useful for understanding ways for cheap, commercial production of these extracts at extensive level under controlled conditions.
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    A molecular epidemiological investigation of multistate outbreaks of Salmonella Enteritidis from clinical and environmental samples in Turkey, 2000 2010
    (2015) Acar, Sümeyra; Atalan, Ekrem; Levent, Belkıs
    Abstract: Background/aim: To analyze interrelated Salmonella Enteritidis strains isolated in Turkey. Fifty-five S. Enteritidis surveillance strains were isolated from human feces and environmental samples from different regions in Turkey between 2000 and 2010. Materials and methods: Clinical isolates were selected from different outbreaks in the Turkish National Reference Laboratory. All isolates were submitted to antimicrobial susceptibility test, plasmid profile analysis, and XbaI-digested pulsed-field gel electrophoresis. Results: The strains were scanned against 20 antibiotics and for 3 of them (amikacin, ciprofloxacin, gentamicin), all strains were found to be sensitive. Five isolates had no plasmid. Most of test strains carried the 57-kb plasmid in common and 15 genotypes were identified among the 55 isolates. Six genotypes were related closely, 3 genotypes were undistinguished, and 6 genotypes were unrelated. Conclusion: To our knowledge, this is the first report on the phenotypic and molecular characterization of S. Enteritidis isolates from both environmental samples and clinical isolates in Turkey.
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    A molecular epidemiological investigation of multistate outbreaks of Salmonella Enteritidis from clinical and environmental samples in Turkey, 2000-2010
    (Tubitak Scientific & Technological Research Council Turkey, 2015) Acar, Sumeyra; Levent, Belkis; Atalan, Ekrem
    Background/aim: To analyze interrelated Salmonella Enteritidis strains isolated in Turkey. Fifty-five S. Enteritidis surveillance strains were isolated from human feces and environmental samples from different regions in Turkey between 2000 and 2010. Materials and methods: Clinical isolates were selected from different outbreaks in the Turkish National Reference Laboratory. All isolates were submitted to antimicrobial susceptibility test, plasmid profile analysis, and XbaI-digested pulsed-field gel electrophoresis. Results: The strains were scanned against 20 antibiotics and for 3 of them (amikacin, ciprofloxacin, gentamicin), all strains were found to be sensitive. Five isolates had no plasmid. Most of test strains carried the 57-kb plasmid in common and 15 genotypes were identified among the 55 isolates. Six genotypes were related closely, 3 genotypes were undistinguished, and 6 genotypes were unrelated. Conclusion: To our knowledge, this is the first report on the phenotypic and molecular characterization of S. Enteritidis isolates from both environmental samples and clinical isolates in Turkey.