Yazar "Aydin, Muhterem" seçeneğine göre listele

Listeleniyor 1 - 7 / 7
  • Küçük Resim Yok
    Öğe
    Beneficial effects of nerolidol on thioacetamide-induced damage of the reproductive system in male rats
    (Allied Acad, 2016) Celik, Huseyin; Camtosun, Ahmet; Ciftci, Osman; Cetin, Asli; Aydin, Muhterem; Gurbuz, Sukru
    In this study, it was aimed to determinate beneficial effects of Nerolidol (NLR) against reproductive toxicity caused by Thioacetamide (TAA). Male, 3-4-months-old, rats (n=32) were divided into four groups. Group-1 was kept as control and given corn oil as carrier. Group-2 received TAA (200 mg/kg, intraperitoneal (i.p.), two times per week) for 3 weeks, in group-3; NRL was orally administered at the dose of 100 mg/kg per every other day by gavages, group-4; 200 mg/kg TAA and 100 mg/kg NRL were given. Thiobarbituric Acid Reactive Substances (TBARS) and reduced Glutathione (GSH) levels, Catalase (CAT), Superoxide Dismutase (SOD) and Glutathione Peroxidase (GPX), sperm parameters and reproductive organs weight were determined. TAA caused a significant rise in TBARS level and a significant reduce in GPX, CAT, SOD and GSH levels in the testicular tissues compared with the control group, while NLR led to significant reduce in lipid peroxidation via decreasing TBARS level and increasing the levels of GPX, CAT, SOD and GSH. Besides, sperm parameters significantly reduced, and pathologic testicular damage increased with TAA exposure. However, these effects of TAA on sperm parameters and histopathological changes were reversed by NLR treatment. In conclusion, our results demonstrate that the management of TAA induced the testicular damage and NLR prevented thioacetamide-induced testicular damage in rats.
  • Küçük Resim Yok
    Öğe
    The effect of aromatase inhibitors against possible testis toxicity in pembrolizumab treated rats
    (Wiley, 2022) Turkmen, Nese Basak; Ciftci, Osman; Taslidere, Asli; Aydin, Muhterem; Eke, Binay Can
    Pembrolizumab is a monoclonal antibody. Anastrozole is an infertility inhibitor of aromatase. Resveratrol is an antioxidant polyphenol in the reproductive system. This study was planned to demonstrate the protective effects of anastrozole and resveratrol against pembrolizumab-induced reproductive damage. Forty-two Sprague-Dawley rats were used in the study. Groups: The control, Pembrolizumab (PEMB), PEMB + Anastrazol (ANAST), PEMB + Resveratrol (RES), RES, and ANAST groups. At the end of the experiment, rats were euthanased under anaesthesia. Tissue samples were taken from rats for biochemical, histological, and ELISA evaluations. Tissues were subjected to routine tissue follow-up for histological analysis. Biochemically, thiobarbituric acid reactive substance (TBARS), glutathione (GSH), catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) levels were measured. Sperm motility, abnormal sperm rate, and epididymal sperm concentration were examined spermatologically. Serum testosterone and programmed cell death-1 (PD-1) levels were measured using the ELISA. TBARS levels were significantly increased and GSH, SOD, GPx, and CAT levels were mitigated in PEMB-treated rats. Histologically; Control, ANAST, and RES groups testis samples were observed with normal histological appearance. Histological damage was detected in seminiferous tubule structures in testicular tissue in the PEMB group. In treatment groups, this damage was decreased. In addition, PD-1 and testosterone levels were evaluated by the ELISA method. ANAST and RES have therapeutic effects against reproductive damage caused by PEMB.
  • Küçük Resim Yok
    Öğe
    Favourable effect of ?-glucan treatment against cisplatin-induced reproductive system damage in male rats
    (Wiley, 2019) Kaya, Kursat; Ciftci, Osman; Aydin, Muhterem; Cetin, Asli; Basak, Nese
    The aim of this study was to investigate the potential beneficial effects of beta-glucan treatment against oxidative, histological and spermatological damage caused by cisplatin on the male reproductive system. Twenty-eight Sprague Dawley male rats were used in the study. The rats were randomly divided into four equal-sized groups: a control group, cisplatin group (7 mg/kg in a single-dose cisplatin administered intraperitoneally), beta-glucan group (beta-glucan given at a dose of 50 mg kg(-1) d(-1) for 14 day) and a cisplatin plus beta-glucan group (cisplatin and beta-glucan administered together at the same dose). Cisplatin administration induced an increase in the level of thiobarbituric acid-reactive substances, a lipid peroxidation indicator. It induced a decrease in enzymatic (superoxide dismutase, catalase and glutathione peroxidase) activities and nonenzymatic (reduced glutathione) antioxidant levels. In addition, cisplatin caused both histological and spermatological damage, as shown by a decrease in sperm motility and epididymal sperm concentrations and an increase in abnormal sperm rates. The beta-glucan treatment improved cisplatin-induced oxidative, histological and spermatological damage. This study revealed that beta-glucan treatment provided prevention against male reproductive system damage caused by cisplatin. These preventative effects were likely due to its antioxidant properties.
  • Küçük Resim Yok
    Öğe
    Investigation of protective effect of ellagic acid in phthalates-induced reproductive damage
    (Taylor & Francis Ltd, 2022) Turkmen, Nese Basak; Ayhan, Idris; Taslidere, Asli; Aydin, Muhterem; Ciftci, Osman
    Phthalates that people are exposed to every day are toxic carcinogenic chemicals with proven harmful effects on growth and reproduction. Ellagic acid (EA) is a polyphenol derivative known for its antioxidant properties. We hypothesized that the possible reproductive damage mechanism of phthalates is oxidative attack and ellagic acid could have a protective effect against radical forms in the body through its antioxidant properties. Thirty-two male rats were randomly divided into 4 groups, with 8 rats in each. Phthalate (DBP) was administered intraperitoneally and EA acid through gastric oral gavage (phthalate group 500 mg/kg/day DBP; EA group 2 mg/kg/day ellagic acid; the treatment group 500 mg/kg/day DBP and 2 mg/kg/day EA). The vehicle of DBP and EA, carboxymethyl cellulose was administered to control group. At the end of 4 weeks the testis tissue samples were taken under mild anesthesia. Tissue malondialdehyde, antioxidant parameters, sperm motility, sperm density and abnormal spermatozoon ratios were determined. Analysis was performed with One Way ANOVA test using SPSS 12.0 program. As a result; it has been shown that DBP causes oxidative damage by increasing the malondialdehyde level and decreasing antioxidant parameters, increased abnormal sperm rate and decreased sperm motility and concentration and histopathological damage so this damage is inhibited by the antioxidant activity of ellagic acid.
  • Küçük Resim Yok
    Öğe
    Investigation of the Protective Effect of Nerolidol on Dehydroepiandrosterone-induced Polycystic Ovary Syndrome in Female Rats
    (Wiley, 2023) Yuce, Hande; Turkmen, Nese Basak; Aydin, Muhterem; Taslidere, Asli; Dogan, Aysegul; Ozek, Dilan Askin; Hayal, Taha Bartu
    [Abstract Not Available]
  • Küçük Resim Yok
    Öğe
    Nerolidol attenuates dehydroepiandrosterone-induced polycystic ovary syndrome in rats by regulating oxidative stress and decreasing apoptosis
    (Pergamon-Elsevier Science Ltd, 2023) Turkmen, Nese Basak; Yuce, Hande; Aydin, Muhterem; Taslidere, Asli; Dogan, Aysegul; Ozek, Dilan Askin; Hayal, Taha Bartu
    Aims: Although nerolidol (NRL) is a naturally occurring sesquiterpene alcohol with many pharmacological ac-tivities, its role in dehydroepiandrosterone DHEA-induced polycystic ovary syndrome PCOS is unknown. This study aims to explore the potential beneficial effects and underlying molecular mechanisms of nerolidol treat-ment on polycystic ovary syndrome.Main methods: Pre-pubertal female Sprague-Dawley rats were randomly assigned into four groups (n = 8/group); group I: control; group II: PCOS; group III: P + NRL; group IV: NRL. Biochemical parameters related to oxidative stress, inflammation, apoptosis, and hormones were estimated in the blood and ovarian tissues. Histopatho-logical, ultrastructural, and immunohistochemical analyses were performed. Bax, P53, Cas-3, and Bcl-2 gene expression levels were detected with RT-PCR. The membrane array analysis detected chemokine, cytokine, and growth factor protein profiles.Key findings: In light of the available data, it can deduce that nerolidol has a significant ameliorating effect on lipid peroxidation, oxidative stress, inflammation, histopathological damage, and apoptosis accompanying PCOS in female rats.Significance: PCOS is not only a reproductive pathology but also a systemic condition and its etiopathogenesis is still not fully understood. Since changes in PCOS have important long-term effects on health, this study evaluated the efficacy of nerolidol, a phytotherapeutic for the control of biochemical, apoptotic, histopathological, and metabolic changes.
  • Küçük Resim Yok
    Öğe
    Protective role of vitamin E against acrylamide-induced testicular toxicity from pregnancy to adulthood: insights into oxidative stress and aromatase regulation
    (Springer, 2024) Uremis, Muhammed Mehdi; Gultekin, Sevinc; Uremis, Nuray; Safak, Tarik; cigremis, Yilmaz; Gul, Mehmet; Aydin, Muhterem
    Acrylamide (ACR) is a toxic chemical frequently encountered in daily life, posing health risks. This study aimed to elucidate the molecular-level mechanism of ACR's toxic effects on testicles and investigate whether Vitamin E can mitigate these effects. A total of 40 adult pregnant rats were utilized, divided into four groups: Control, ACR, Vitamin E, and ACR + Vitamin E. ACR and Vitamin E were administered to the mother rats during pregnancy and lactation, and to the male offspring until the 8th week post-birth. Serum hormone levels, oxidant-antioxidant parameters, histopathological examination of testicular tissue, and mRNA and protein levels of the testicular and liver aromatase gene were analyzed. Spermiogram analysis was conducted on the collected sperm samples from the male offspring. The results revealed that ACR exposure adversely affected hormone levels, oxidant-antioxidant parameters, histological findings, as well as aromatase gene and protein expressions. However, Vitamin E administration effectively prevented the toxic effects of ACR. These findings demonstrate that ACR application significantly impairs the reproductive performance of male offspring rats by increasing liver aromatase activity.