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Öğe Chemical composition, antimicrobial and antioxidant properties of Thymus eigii M. Zohary & P. H. Davis essential oil(2010) Azaz A.D.; Kucukbay Z.; Celen S.; Kuyumcu E.; Yildiz B.The family Lamiaceae is represented by 45 genera, 546 species and 730 taxa in Turkey. The genus Thymus is represented in Turkey by 38 species; the ratio of endemism in the genus is 53%. Thymus eigii samples collected from Hatay were subjected to hydrodistillation to yield essential oils that were subsequently analysed by Gas Chromatography (GC) and Gas Chromatography/Mass Spectrometry (GC/MS). The main constituents of the oils were identified and antimicrobial and antioxidant bioassays were applied. The T.eigii white flowers oil contained carvacrol (43.63%), p-cymene (20.55 %) and thymol (16.06%); T.eiigi with purple flowers oil contained geranyl acetate (15.66 %), p-cymene (14.62%) and thymol (11.48%) as the main components. All tested microorganisms were inhibited by the essential oil samples. Antioxidant activity was tested using the 2,2-diphenyl-l-picrylhydrazyl (DPPH) radical-scavenging method. The antioxidant activity of the tested essential oils were slightly lower than butylated hydroxyanisole (BHA) and so the essential oils can be considered effective natural antioxidants. © Essential Oil Resource Consultants. All rights reserved.Öğe Chemical composition, antimicrobial and antioxidant properties of Thymus haussknechtii Velen. essential oil(2012) Celen S.; Azaz A.D.; Kucukbay F.Z.The chemical composition, the antimicrobial and antioxidant activities of essential oil of Thymus haussknechtii has been investigated. T. haussknechtii collected from Erzincan was subjected to hydrodistillation to yield essential oil which was subsequently analyzed by Gas Chromatography (GC) and Gas Chromatography/Mass Spectrometry (GC/MS). The main components were 1,8-cineole (23.6 %), trans-verbenol (6.6 %), camphor (6.12 %) and caryophyllene oxide (6 %). The antimicrobial activity of the essential oil was assessed by both the disc diffusion and microbroth dilution methods. All tested microorganisms were inhibited by essential oil sample. The essential oil is also active on test fungi. Aspergillus flavus and A. niger were sensitive to the investigated oil with MIC values of 500 ?g/mL. The antioxidant activity of the essential oil (100-1000 ?g/mL) was determined by means of the DPPH radical-scavenging method. At 1000 ?g/mL concentration of the essential oil T. haussknechtii, 35.11 ± 0.22 % DPPH was scavenging.Öğe Composition and the in vitro antimicrobial activities of the essential oils of Achillea wilhelmsii C. Koch. and Achillea lycaonica Boiss & Heldr.(Chemical Publishing Co., 2008) Azaz A.D.; Arabaci T.; Sangun M.K.; Yildiz B.The genus Achillea (Asteraceae) is represented by 42 species (48 taxa) in Turkey and 22 of which are endemic to Turkey. Achillea wilhelmsii and Achillea lycaonica collected from different localities in Turkey, were subjected to hydrodistillation to yield essential oils which were subsequently analyzed by GC and GC/MS. The main constituents of the oils were identified and antimicrobial bioassays were applied. Camphor (39.62 %) was the main component in the oil of Achillea wilhelmsii. The oil of Achillea lycaonica contained L-camphor (43.19 %) as main constituent. All tested microorganisms were inhibited by the essential oils. On the other hand both essential oils showed strong antifungal activity against Alternaria brassicola than other tested microfungi.Öğe Essential oil composition and antimicrobial activities of achillea biserrata M. Bieb. and achillea salicifolia besser subsp. salicifolia collected in Turkey(Chemical Publishing Co., 2009) Azaz A.D.; Arabaci T.; Sangun M.K.Aerial parts of Achillea biserrata (collected from Gumushane between Trabzon) and A. salicifolia subsp. salicifolia (collected from Ardahan between Gole) were subjected to hydrodistillation to yield their essential oils and were analyzed by GC and GC-MS. The main compo- nents of tested essential oils were camphor (36.80-55.26 %), 1,8-cineol (19.35-22.75 %), camphene (16.41-3.23 %) and artemisia alcohol (14.28-3.17 %), respectively. The antibacterial and antifungal activities of tested essential oils were evaluated against Escherichia coli ATCC 25292, Staphylococcus aureus ATCC 6538, Proteus vulgaris NRRL 123, Pseudomonas aeruginosa ATCC 27853, Enterobacter aemgenes NRRL 3567, Listeria monocystogenes ATCC 7644, Serratia marcescens, Candida albicans, Penicilliwn expansum, Aspergillus flavus, A. niger and Alternaria brassicola. All tested bacteria and Candida albicans were inhibited by the essential oils. The essential oils showed weak antifungal activity against all microfungi tested. Nevertheless, it is found that P. expansum was not inhibited by any essential oils used in this study. Aspergillus niger was also not inhibited by A biserrata. Alternaria brassicola was the most sensitive microfungi against all the tested essential oils.