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Öğe Administration of Acute Agomelatine Reduces Increased Oxidative Stress Due to Experimental Renal Ischemia / Reperfusion Damage in Rats(Wiley, 2019) Aykora, Damla; Ozturk, Dilara Altay; Tanbek, Keyser; Bahar, Mehmet Refik; Tekin, Suat; Sandal, Suleyman[Abstract Not Available]Öğe Chemical and biological characterization of sulfated chitosan oligomer as heparin mimics(Sage Publications Ltd, 2021) Kocabay, Samet; Bahar, Mehmet Refik; Tekin, Suat; Akkaya, Recep; Akkaya, BirnurIn the present study, chitosan oligomer was modified to sulfated chitosan oligomer (ShCsO) to mimic heparin. Its chemical structure was determined by infrared spectroscopy (FT-IR), X-ray diffractometry (XRD), differential scanning calorimetry (DSC), and thermogravimetric analysis. The results showed that the FT-IR spectrum band at 799 cm(-1) corresponds to C-O-S and that at 1212 cm(-1) corresponds to S=O bond stretching, which prove that the sulfate groups are incorporated into chitosan oligomer successfully. The antimicrobial activity of ShCsO against to Bacillus subtilis in 1% concentration was 89.1 +/- 1.7%. The IC50 (mu g/ml) of ShCsO was 67.75, 56.07, 85.47, and 84.68 for A2780, MCF-7, DU-145, and HepG2, respectively. The results show that this newly synthesized material is a potential candidate to heparin-like chitosan derivatives. According to the literature, it was the first time that chitosan oligomer was modified to mimic heparin.Öğe (E)-1-(4-Hydroxyphenyl)-3-(substituted-phenyl) prop-2-en-1-ones: Synthesis, In Vitro Cytotoxic Activity and Molecular Docking Studies(Slovensko Kemijsko Drustvo, 2022) Sirka, Lutfiye; Dogan, Hacer; Bahar, Mehmet Refik; Caliskan, Eray; Tekin, Suat; Uslu, Harun; Koran, KenanA series of chalcone compounds (2-11) were designed and synthesized to determine their cytotoxic effects. The structures of 2-11 were fully characterized by their physical and spectral data. The in vitro cytotoxic effects of 2-11 were evaluated against human ovarian cancer (A2780), breast cancer (MCF-7) and prostate cancer (PC-3 and LNCaP) cell lines. The activity potentials of compounds were further evaluated through molecular docking studies with AutoDock4 and Vina softwares. All the compounds (except compound 5) showed significant cytotoxic effects at high doses in all cancer cell lines. Among all the compounds studied, one compound i.e. compound 2 demonstrated dose-dependent activity, particularly against A2780/LNCaP cancer cell lines. The most effective compounds 8, 9, 10 and 11 reduced the cell viability of A2780, MCF-7, PC-3 and LNCaP cells by 50-98%, while other compounds 2, 4 and 7 reduced the cell viability of A2780 cells by 70-90% at concentrations of 50 and 100 mu M.Öğe Effect of Intracerebroventricular MOTS-c Infusion on Peripheral Uncoupling Proteins in Rats(Karger, 2021) Bahar, Mehmet Refik; Tekin, Suat; Beytur, Asiye; Onalan, Ebru Etem; Colak, Cemil; Sandal, Suleyman[Abstract Not Available]Öğe Effects of intracerebroventricular MOTS-c infusion on thyroid hormones and uncoupling proteins(Springer Heidelberg, 2023) Bahar, Mehmet Refik; Tekin, Suat; Beytur, Asiye; Onalan, Ebru Etem; Ozyalin, Fatma; Colak, Cemil; Sandal, SuleymanThis study was conducted to determine the possible effects of intracerebroventricular MOTS-c infusion on thyroid hormones and uncoupling proteins (UCPs) in rats. Forty male Wistar Albino rats were divided into 4 groups with 10 animals in each group: control, sham, 10 and 100 mu M MOTS-c. Hypothalamus, blood, muscle, adipose tissues samples were collected for thyrotropin-releasing hormone (TRH), UCP1 and UCP3 levels were determined by the RT-PCR and western blot analysis. Serum thyroid hormone levels were determined by the ELISA assays. MOTS-c infusion was found to increase food consumption but it did not cause any changes in the body weight. MOTS-c decreased serum TSH, T3, and T4 hormone levels. On the other hand, it was also found that MOTS-c administration increased UCP1 and UCP3 levels in peripheral tissues. The findings obtained in the study show that central MOTS-c infusion is a directly effective agent in energy metabolism.Öğe In Vitro Cytotoxic and Genotoxic Properties of Hexa Substituted New Organophosphazene Compounds(Wiley, 2019) Dogan, Hacer; Bahar, Mehmet Refik; Caliskan, Eray; Koran, Kenan; Tekin, Suat; Sandal, Suleyman; Gorgulu, Ahmet Orhan[Abstract Not Available]Öğe Intracerebroventricular MOTS-C Infusion Suppresses Thyroid Hormone Secretion in Rats(Wiley, 2019) Bahar, Mehmet Refik; Tekin, Suat; Beytur, Asiye; Ozyalm, Fatma; Onalan, Ebru Etem; Sandal, Suleyman[Abstract Not Available]Öğe İntraserebroventriküler mots-c infüzyonunun hipotalamus-hipofiz-tiroid aksı ve enerji kullanımı üzerindeki etkileri(İnönü Üniversitesi, 2020) Bahar, Mehmet RefikAmaç: MOTS-c 2015 yılında keşfedilmiş, 16 aminoasitli peptit yapılı bir hormondur. Yapılan çalışmalarla MOTS-c'nin obezite ve insülin direncini azalttığı ve hücre metabolizması arttırdığı rapor edilmiştir. Bu çalışma sıçanlara icv MOTS-c infüzyonunun HHT aksı ve enerji kullanımı üzerindeki etkilerini araştırmak amacıyla yapıldı. Materyal ve Metot: 40 adet Wistar-Albino cinsi erkek sıçan 4 gruba ayrıldı (n=10). Kontrol grubu dışındaki sıçanların, lateral ventrikülerine 14 gün süresince 5µl/saat (Sham grubuna yBOS, uygulama gruplarına 10 ve 100 µM MOTS-c) infüzyon gerçekleştirildi. Deney süresince sıçanların günlük vücut ağırlığı ve yem tüketimi ölçüldü. Deney sonunda sıçanlar dekapite edilerek hipotalamus, kas, kan ile beyaz ve kahverengi yağ doku örnekleri toplandı. Hipotalamus dokusunda TRH, beyaz ve kahverengi yağ dokuda UCP1, kas (biceps) dokuda ise UCP3 mRNA seviyesi RT-PZR yöntemi ile belirlendi. Western blot analiz yöntemi ile de beyaz ve kahverengi yağ dokuda UCP1, kas dokuda ise UCP3 protein düzeyi belirlendi. Alınan kan örneklerinden ELISA yöntemi kullanılarak serum TSH, T3 ve T4 hormon seviyeleri belirlendi. Bulgular: MOTS-c infüzyonunun sıçanlarda yem tüketimini arttırdığı (p<0.05) vücut ağırlığında ise değişikliğe neden olmadığı belirlendi. MOTS-c infüzyonu sıçanların TRH mRNA düzeyinde değişikliğe neden olmazken, serum TSH, T3 ve T4 hormon seviyesini azalttığı görüldü (p<0.05). Öte yandan MOTS-c'nin sıçanların beyaz ve kahverengi yağ dokularında UCP1, kas dokusunda ise UCP3 mRNA ve protein düzeyini arttırdığı tespit edildi (p<0.05). Sonuç: Bu çalışmanın sonuçları MOTS-c'nin HHT aksında görev alan dokular ile kas ve yağ dokusunda (beyaz ve kahverengi) fizyolojik roller üstlendiğini göstermektedir. Özellikle HHT aksında baskılayıcı tarzda ortaya çıkan etki ile periferal enerji kullanımındaki aktive edici etkilerini hangi fizyolojik yolaklar üzerinden gösterdiği halen gizemini korumaktadır. Anahtar Kelimeler: MOTS-c, TRH, TSH, T3, T4, UCP1, UCP3Öğe Synthesis and spectroscopic characterizations of hexakis[(1-(4 '-oxyphenyl)-3-(substituted-phenyl)prop-2-en-1-one)]cyclotriphosphazenes: their in vitro cytotoxic activity, theoretical analysis and molecular docking studies(Taylor & Francis Inc, 2022) Dogan, Hacer; Bahar, Mehmet Refik; Caliskan, Eray; Tekin, Suat; Uslu, Harun; Akman, Feride; Koran, KenanThe hexachlorocyclotriphosphaze compound (N3P3Cl6, HCCP) was reacted with excess (E)-(1-(4 '-oxyphenyl)-3-(substituted-phenyl)prop-2-en-1-ones (2-11) to produce hexakis[(1-(4-oxyphenyl)-3-(substituted-phenyl)prop-2-en-1-one)]cyclotriphosphazenes (CP 2-11). The structures of products (CP 2-11) were confirmed using elemental analysis, FT-IR, MS spectral analysis as well as P-31, H-1 and C-13-APT NMR techniques and their thermal properties determined by TGA and DSC techniques. The HOMO-LUMO energy gap and chemical reactivity identifiers were calculated and HOMO and LUMO images were viewed. According to the calculations, all the chemical potential values of CP 2-11 are negative and it shown that the molecules are stable. The in vitro cytotoxic of CP 2-11 investigated and their activity potentials were evaluated by molecular docking studies with Autodock Vina softwares. CP 2-11 compounds were found to demonstrate cytotoxic activity against human cancer cell lines (A2780, LNCaP and PC-3). The CP 2-11 compounds reduced the cell viability against all cancer cell lines in the range 36%-90% especially. The results showed that these compounds are powerful candidate molecules for pharmaceutical applications.
