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2-Morpholinoethyl-substituted N-heterocyclic carbene (NHC) precursors and their silver(I)NHC complexes: synthesis, crystal structure and in vitro anticancer properties
(Springer, 2018) Aktas, Aydin; Kelestemur, Unzile; Gok, Yetkin; Balcioglu, Sevgi; Ates, Burhan; Aygun, Muhittin
In this study, a series of unsymmetrically 2-morpholinoethyl-substituted benzimidazolium salts and their Ag(I)NHC complexes were synthesized. The 1,3-dialkylbenzimidazolium salts (1a-d) were synthesized in dimethylformamide at 80 A degrees C temperature from the N-(2-morpholinoethyl)benzimidazole and alkyl halides. The Ag(I)NHC complexes (2a-d) were synthesized in dichloromethane at room temperature from the benzimidazolium salts and Ag2O. All compounds were characterized by spectroscopic techniques (NMR and FT-IR) and elemental analyses. Also, the salt 1c and complex 2c were characterized by single-crystal X-ray crystallography. Anticancer activities of 2-morpholinoethyl-substituted benzimidazolium salts and Ag(I)NHC complexes were investigated against the MCF-7 breast cancer cell line, and the IC30 and IC50 values of these compounds were found to be in the range of 241-490 and 6-14 A mu M, respectively.
Aliphatic Polyurethane Films Based on Hexamethylene Diisocyanate and Saccharides for Biocompatible Transparent Coating on Optic Medical Devices
(Wiley-V C H Verlag Gmbh, 2022) Gurses, Canbolat; Karaaslan-Tunc, Merve Goksin; Kelestemur, Unzile; Balcioglu, Sevgi; Gulgen, Selam; Koytepe, Suleyman; Ates, Burhan
Polyurethanes are widely used in daily life, from the textile industry to medical applications. In this study, a biocompatible and transparent coating from polyethylene glycol-based polyurethanes including different type of saccharide (xylose as monosaccharide, sucrose as disaccharide, or starch as polysaccharide) is prepared. Prepared optically transparent polyurethanes are characterized with different instrumental analysis techniques and contact-angle measurements. Thermogravimetric analysis data allow understanding that the synthesized saccharide based polyurethanes are resistant to high temperatures, which means that medical devices can be easily coated with one of the saccharide-based polymers during the manufacturing process. The study results also show that synthesized polyurethane samples having a different kind of saccharides present high biocompatibility and transparency properties via In vitro cytotoxicity analysis and optic transparency tests. For xylose, sucrose, or starch containing polyurethanes, the tranmittance values are 97.72%, 85.20%, and 89.23%, respectively. In addition, scanning electron microscope images show that the samples also have smooth surfaces which can be used for proper coating of medical devices. Consequently, this manuscript addresses synthesis and characterization of the optically transparent polyurethanes based on specific saccharides for biocompatible and transparent surfaces especially in optic medical devices such as colonoscopy applications and laparoscopic detection probes.
Benzotriazole functionalized N-heterocyclic carbene-silver(I) complexes: Synthesis, cytotoxicity, antimicrobial, DNA binding, and molecular docking studies
(Pergamon-Elsevier Science Ltd, 2018) Onar, Gulnihan; Karatas, Mert Olgun; Balcioglu, Sevgi; Tok, Tugba Taskin; Gurses, Canbolat; Kilic-Cikla, Isin; Ozdemir, Namik
In this study, six [Ag(NHC)(2)](+)[AgCl2](-) type silver complexes were synthesized by the reaction of corresponding carbene precursor and Ag2O. One [Ag(NHC)(2)]+NO3- type complex was synthesized by the anion exchange reaction of corresponding silver-NHC and NaNO3. The synthesized complexes were characterized by H-1 NMR, C-13 NMR and IR spectroscopic methods, and elemental analysis. X-ray crystal structure of 5a was also reported. Cytotoxicities of all compounds were evaluated against human breast (MCF-7) and colorectal (Caco-2) cancer cell lines and non-cancer mouse fibroblast (L-929) cell lines. All complexes performed stronger activity against both cancer cell lines than standard compound cisplatin while complex 3b performed nearly equal cytotoxicity to cisplatin against non-cancer L-929. Antimicrobial effects of all compounds were evaluated against Escherichia coli, Bacillus subtilis and Candida albicans and good activities were observed. The docking results indicated that complex 3b might be classified as druggable molecule in drug design. DNA binding study also demonstrates that 3b complex has an interaction ability to DNA. Combination of experimental and molecular docking results revealed that reported complexes are promising structures and deserve further research as anticancer drugs. (C) 2018 Elsevier Ltd. All rights reserved.
Biodegradable non-aromatic adhesive polyurethanes based on disaccharides for medical applications
(Elsevier Sci Ltd, 2014) Ates, Burhan; Koytepe, Suleyman; Karaaslan, Merve Goksin; Balcioglu, Sevgi; Gulgen, Selam
This work addresses synthesis, characterization, adhesive properties, protein adsorption and in vitro biodegradation of non-aromatic polyurethanes (NPUs). The following reactants were used in the syntheses of polyurethanes (PUs): non-aromatic 4,4'-Methylenebis(cyclohexyl isocyanate) (MCI), disaccharides (maltose and sucrose) and polyethylene glycol 200 (PEG 200). The NPUs exhibited low glass transition temperature (T-g) and good thermal stability. The dynamic contact angles on NPUs were influenced by the ratios of PEG 200-disaccharide monomer and the hard segment of the NPUs. The highest adhesion strength in whole polyurethanes was found as 307.6 +/- 50.1 kPa for 15% sucrose containing polyurethane. Protein adsorptions of polyurethane films were measured as average 10 pg Bovine Serum Albumin (BSA)/cm(2) and 5 mu g fibrinogen/cm(2) for maltose, and 15 mu g BSA/cm(2) and 5 mu g fibrinogen/cm(2) for sucrose. Biodegradability ratios of NPUs were in the range of 15-35% for 8 weeks depending on the disaccharides containing NPUs. (C) 2013 Elsevier Ltd. All rights reserved.
Biomedical applications of hybrid polymer composite materials
(Woodhead Publ Ltd, 2017) Ates, Burhan; Koytepe, Suleyman; Balcioglu, Sevgi; Ulu, Ahmet; Gurses, Canbolat
[Abstract Not Available]
Biomimetic approach to tunable adhesion of polyurethane adhesives through Fe3+ crosslinking and hydrophobic tween units with balance of adhesion/cohesion forces
(Elsevier Sci Ltd, 2019) Ates, Burhan; Koytepe, Suleyman; Balcioglu, Sevgi; Karaaslan, Merve Goksin; Kelestemur, Unzile; Gulgen, Selam; Ozhan, Onural
Biocompatible adhesives have some limitations such as weak adhesion and low flexibility. To overcome these limitations, we described multiple strategies to provide strong adhesion and high flexibility through Tweens, chlorogenic acid (CLA) and polyethylene glycol (PEG) by reducing excessive interaction between tissue and the adhesive. We synthesized polyurethane-based adhesives using aliphatic 4,4'-methylenebis(cyclohexyl isocyanate) (HMDI), PEG, CLA and Tween units. Hydrophobic side chains in polymer resulted in lower Tg (- 36.95-30.36 degrees C) which indicated more flexibility. The highest adhesion strengths were found as almost 346 kPa for bare polyurethane and 492 kPa for chelated polymer (PU-T40-CLA-15% (5% Tween 40, 15% chlorogenic acid and 80% PEG 200 containing polymer)) with FeCl3. The addition of Tween units provided more stable structure to polymers which proved with in vitro erosion studies. Relatively low erosion values were seen as 5.7, 5.6 and 8.2% in PU-T40-CLA-5% (15% Tween 40, 5% chlorogenic acid and 80% PEG 200 containing polymer), PU-T40-CLA-10% (10% Tween 40, 10% chlorogenic acid and 80% PEG 200 containing polymer), and PU-T40-CLA-15% (5% Tween 40, 15% chlorogenic acid and 80% PEG 200 containing polymer), respectively. In vitro biocompatibility results showed high cell viability in PU-T40-15% as more than 100%. Overall, our findings indicated that these material designs (PU-T-CLAs) provided to overcome the significant challenges of tissue adhesives by improving the flexible character and adhesive strength of the adhesives.
Cellulose acetate encapsulated upconversion nanoparticles - A novel theranostic platform
(Elsevier, 2021) Topel, Seda Demirel; Balcioglu, Sevgi; Ates, Burhan; Asilturk, Meltem; Topel, Onder; Ericson, Marica B.
Luminescent upconversion nanoparticles (UCNPs) are of great interest in a wide range of nanotechnological applications, particularly in the biomedical area like imaging and therapy but their biocompatibility and stability pose major challenges hampering progression towards further pharmaceutical applications. Herein, we present a biocompatible theranostic platform enabling simultaneous diagnosis and drug delivery consisting of UCNPs encapsulated with cellulose acetate (CA), a biocompatible polymer. Luminescence properties of UCNPs in the developed theranostic platform remain stable even after encapsulation. The size of the CA capsules, ranging from micro- to nano-sized particles, can easily be tuned by adjusting the stirring rate during encapsulation. Doxorubicin, a well-known chemotherapeutic drug, onto the CA nanocapsules containing UCNPs (UCNP-CA nanocapsules) was loaded with up to -63 % efficiency and acid-induced release (similar to 47 %) obtained at pH 3.6 and 5.5. It was found that encapsulation decreased toxicity of UCNPs as confirmed in a cellular assay (L-929 and MCF-7 cell lines). Taken together, the developed UCNP-CA nanocapsules serve as a highly interesting novel theranostic platform, combining the biocompatible optical properties of UCNP, with reduced cell toxicity and drug encapsulating properties of CA. The proposed system could be subject for further refinement and exploration.
Chlorogenic Acid Containing Bioinspired Polyurethanes: Biodegradable Medical Adhesive Materials
(Taylor & Francis As, 2015) Ates, Burhan; Koytepe, Suleyman; Karaaslan, Merve Goksin; Balcioglu, Sevgi; Gulgen, Selam; Demirbilek, Murat; Denkbas, Emir Baki
Highly adhesive bioinspired polyurethanes (PUs) based on the chlorogenic acid (CLA) were prepared from 4,4'-methylenebis (cyclohexyl isocyanate) (MCI) and polyethylene glycol 200 (PEG 200). The polyurethanes exhibited the lowest glass transition temperature (T-g), improved thermal stability and good adhesive properties. The highest adhesion strength was found as 373.3 +/- 47.5 kPa for 10% CLA containing PUs. The polyurethanes were observed to be biodegradable and were in the range of 19-24% for 8 weeks as depending on chlorogenic acid containing of PUs. As a result, prepared biocompatible-adhesive bioinspired polyurethanes are good candidates for medical applications as a tissue adhesive material. [GRAPHICS] .
Cytotoxic and apoptotic potentials of four native apricot varieties from Malatya, Turkey: A comprehensive evaluation
(Wiley, 2025) Kelestemur, Unzile; Balcioglu, Sevgi; Ates, Burhan
In recent years, the role of functional foods in addressing various health issues, including cancer, has gained significant attention. Among these, the cytotoxic and apoptotic properties of Malatya apricots hold particular interest for their potential therapeutic benefits. This study focused on exploring the effects of methanol and acetone extracts from four popular Malatya apricot varieties-Hac & imath;halilo & gbreve;lu, Hasanbey, Kabaa & scedil;& imath;, and So & gbreve;anc & imath;-on cancer cell lines. The extracts were derived from apricots in their raw, ripe, and dried form, which can be consumed in three ways, and were tested for their cytotoxic and apoptotic activities against MCF-7 and Caco-2 cell lines using 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide, wound scratch, and acridine orange/ethidium bromide (AO/EB) staining tests. Additionally, we assessed the total phenolic and flavonoid contents of the extracts. Our findings revealed that the acetone extracts of dried and raw Kabaa & scedil;& imath; apricots exhibited the lowest IC50 values against the MCF-7 cell line over 24 and 48 h, with values of 3.9 and 0.5 mg/mL, respectively. Similarly, in the Caco-2 cell line, the dried Kabaa & scedil;& imath; apricot extracts showed the lowest IC(50)values of 3.59 and 1.95 mg/mL for the same time frames. In the wound scratch assay, utilizing apricot extracts at their lowest IC50 values revealed significant differences in cell migration inhibition between treated and control groups. For Caco-2 cells, the control group's wound closure was around 70%, whereas apricot extract treatment resulted in 10%-30% opening after 24 h, indicating a strong antiproliferative effect. Similarly, in MCF-7 cells, the control group showed approximately 80% wound closure, in contrast to the 0%-12% opening observed in cells treated with apricot extract, further emphasizing the extracts' potent inhibitory impact on cell proliferation. Additionally, morphological evidence of cell death and apoptosis was observed in the images obtained through the AO/EB staining method, conducted to showcase the cytotoxic and apoptotic effects of the apricot extract. Apricot extracts consistently demonstrated strong cytotoxic and apoptotic effects on both Caco-2 and MCF-7 cell lines, underscoring Malatya apricots' potential as a functional food component with promising therapeutic properties against cancer.
Cytotoxicity, Radical Scavenging, Antioxidant Properties and Chemical Composition of the Essential Oil of Satureja cilicica P.H. Davis from Turkey
(Marmara Univ, Fac Pharmacy, 2017) Arabaci, Turan; Uzay, Gizem; Kelestemur, Unzile; Karaaslan, Merve Goksin; Balcioglu, Sevgi; Ates, Burhan
Satureja cilicica P. H. Davis is an endemic species of Lamiaceae, distributed in Turkey. In this study, the essential oil of S. cilicica was obtained by hydrodistillation from the aerial parts. The essential oil was analyzed by GC/MS and the main constituents are identified. Radical scavenging capacities of oil obtained from S. cilicica were determined by using DPPH and ABTS radical scavenging assays. In addition, ferric-reducing power of the essential oil was detected. Cytotoxic activity of the essential oil was determined by MTT assay after 24 h treatment of MCF-7. The major components of the essential oil are p-cymene (17.68%), carvacrol (14.02%), gamma-terpinene (11.23 %) and thymol (8.76%). Radical scavenging capacities of the essential oil were measured as 3.28 +/- 0.02 and 238.15 +/- 3.59 mg Trolox equivalent per milliliter of oil in term of DPPH and ABTS radical scavenging, respectively. Ferric-reducing power of essential oil was found 39.76 +/- 3.66 mg Trolox equivalent per milliliter of essential oil. Growth inhibition effect of essential oil against MCF-7 cancer cell is established. According to the results, essential oil of S. cilicica exhibit low cytotoxic activity, with IC50 values, with IC50 value of 268 mu g/mL. The findings of the analyzed species are compared with the results of previous studies.
Design of Xylose-Based Semisynthetic Polyurethane Tissue Adhesives with Enhanced Bioactivity Properties
(Amer Chemical Soc, 2016) Balcioglu, Sevgi; Parlakpinar, Hakan; Vardi, Nigar; Denkbas, Emir Baki; Karaaslan, Merve Goksin; Gulgen, Selam; Taslidere, Elif
Developing biocompatible tissue adhesives with high adhesion properties is a highly desired goal of the tissue engineering due to adverse effects of the sutures. Therefore, our work involves synthesis, characterization, adhesion properties, protein adsorption, in vitro biodegradation, in vitro and in vivo biocompatibility properties of xylose-based semisynthetic polyurethane (NPU-PEG-X) bioadhesives. Xylose-based semisynthetic polyurethanes were developed by the reaction among 4,4'-methylenebis(cyclohexyl isocyanate) (MCI), xylose and polyethylene glycol 200 (PEG). Synthesized polyurethanes (PUs) showed good thermal stability and high adhesion strength. The highest values in adhesion strength were measured as 415.0 +/- 48.8 and 94.0 +/- 2.8 kPa for aluminum substrate and muscle tissue in 15% xylose containing PUs (NPU-PEG-X-15%), respectively. The biodegradation of NPU-PEG-X-15% was also determined as 19.96 +/- 1.04% after 8 weeks of incubation. Relative cell viability of xylose containing PU was above 86%. Moreover, 10% xylose containing NPU-PEG-X (NPU-PEG-X-10%) sample has favorable tissue response, and inflammatory reaction between 1 and 6 weeks implantation period. With high adhesiveness and biocompatibility properties, NPU-PEG-X can be used in the medical field as supporting materials for preventing the fluid leakage after abdominal surgery or wound closure.
Detection of Diazinon Organophosphates Using Magnetoelastic Sensor
(Ieee-Inst Electrical Electronics Engineers Inc, 2022) Atalay, Selcuk; Ates, Burhan; Balcioglu, Sevgi; Inan, Orhan Orcun; Kolak, Seda; Simsek, Murat; Kolat, Veli Serkan
In this study, 2826MB (Fe40Ni38Mo4B18) amorphous ferromagnetic ribbons were used as a magnetoelastic sensor (MES) to detect diazinon organophosphates (OP). Since enlarging the sensor surface area makes a significant contribution to the sensitivity of the sensor, nanofibers were coated on the sensor surface, and then, the nanofibers were functionalized with glutaraldehyde (GA) in order to covalently bind acetylcholinesterase (AChE) enzyme to the surface. It was found that binding of OPs to the AChE enzyme at the functional amorphous ribbon surface, a large change in the resonance frequency was observed. The results showed that functionalized MES shows a linear change according to the amount of OP detected in the range of 0-140 nL or 0-150 ppm in the solution.
Diagnosis, Bacterial Density, Food, and Agricultural Applications of Magnetoelastic Biosensors: Theory, Instrumentation, and Progress
(Springer, 2024) Balcioglu, Sevgi; Inan, Orhan Orcun; Kolak, Seda; Ates, Burhan; Atalay, Selcuk
Magnetoelastic biosensors have emerged as a promising technology for the sensitive and label-free detection of a wide range of biological analytes. These biosensors use the magnetoelastic effect, which describes how the mechanical properties of magnetostrictive materials change in response to a magnetic field. This effect is utilized to detect biological analytes by immobilizing specific recognition elements, such as antibodies or nucleic acids, on the magnetoelastic material's surface. The binding of target analytes to the recognition elements induces a mass change, leading to a shift in the resonance frequency of the magnetoelastic material. Magnetoelastic biosensors find applications across various fields, including medical diagnostics, environmental monitoring, and food safety. In medical diagnostics, they offer rapid and sensitive capabilities for detecting pathogens, biomarkers, and toxins. For environmental monitoring, they demonstrate the ability to detect pollutants and heavy metals. Furthermore, in ensuring food safety and quality, magnetoelastic biosensors detect allergens, pathogens, and contaminants effectively. Ongoing research and technological advancements suggest that these biosensors hold great potential for revolutionizing various fields, including healthcare, environmental monitoring, and food safety, contributing to improved disease diagnosis, environmental protection, and public health. This review article provides an overview of the principles, fabrication methods, diagnosis, bacterial density, food, and agricultural applications of magnetoelastic biosensors.
Electrospun Polycaprolactone (PCL)/Microbial Chondroitin Sulfate (CS)-Based Transdermal Patches for Optimized Ribociclib Delivery
(Amer Chemical Soc, 2025) Gunduz Tavlar, Nermin; Balcioglu, Sevgi; Erenler, Ayse Sebnem; Ates, Burhan
Breast cancer remains the most prevalent cancer worldwide and a leading cause of mortality in women, demanding advanced drug delivery strategies. Ribociclib, a CDK4/6 inhibitor used in hormone-dependent breast cancer therapy, is effective but requires high oral doses, leading to severe systemic toxicity. To overcome this limitation, we developed electrospun PCL/CS-based transdermal patches designed to enhance drug bioavailability, prolong half-life, and minimize side effects. Extensive characterization confirmed the structural integrity and performance of the patches, demonstrating high swelling capacity (81-93%), superior thickness uniformity (95-100%), and exceptional folding endurance (1000-1540 cycles). The patches exhibited excellent weight homogeneity (92-99%) for consistent drug distribution, while adhesion strength (144-386 kPa) ensured stable skin attachment. HPLC analysis revealed sustained Ribociclib release (similar to 95% over 48 h), and ex vivo rat skin diffusion studies confirmed high permeability (68-81%), indicating effective transdermal absorption. Drug retention efficiency exceeded 95% in both the reservoir and drug release layers. Biocompatibility assessments with L-929 fibroblasts demonstrated excellent cell viability (90-95%), while MCF-7 breast cancer cells exhibited potent cytotoxicity (93-94%), comparable to standard Ribociclib treatment. Despite the therapeutic potential of transdermal drug delivery systems (TDDS), Ribociclib has been scarcely explored in this context. This study pioneers a promising alternative for controlled, sustained drug release, potentially revolutionizing breast cancer treatment by improving patient compliance, reducing systemic toxicity, and enhancing therapeutic outcomes.
Fast Curing Multifunctional Tissue Adhesives of Sericin-Based Polyurethane-Acrylates for Sternal Closure
(Amer Chemical Soc, 2022) Balcioglu, Sevgi; Noma, Samir Abbas Ali; Ulu, Ahmet; Karaaslan-Tunc, Merve Goksin; Ozhan, Onural; Koytepe, Suleyman; Parlakpinar, Hakan
The use of wire cerclage after sternal closure is the standard method because of its rigidity and strength. Despite this, they have many disadvantages such as tissue trauma, operatorinduced failures, and the risk of infection. To avoid complications during sternotomy and promote tissue regeneration, tissue adhesives should be used in post-surgical treatment. Here, we report a highly biocompatible, biomimetic, biodegradable, antibacterial, and UV-curable polyurethane-acrylate (PU-A) tissue adhesive for sternal closure as a supportive to wire cerclage. In the study, PU-As were synthesized with variable biocompatible monomers, such as silk sericin, polyethylene glycol, dopamine, and an aliphatic isocyanate 4,4'-methylenebis(cyclohexyl isocyanate). The highest adhesion strength was found to be 4322 kPa, and the ex vivo compressive test result was determined as 715 kPa. The adhesive was determined to be highly biocompatible (on L929 cells), biodegradable, and antibacterial (on Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus bacteria). Finally, after opening the sternum of rats, the adhesive was applied to bond the bones and cured with UV for 5 min. According to the results, there was no visible inflammation in the adhesive groups, while some animals had high inflammation in the cyanoacrylate and wire cerclage groups. These results indicate that the adhesive may be suitable for sternal fixation by preventing the disadvantages of the steel wires and promoting tissue healing.
In vitro antioxidant and antimicrobial activities and therapeutic effects of Rhizopogon luteolus Fr. extract on cecal ligation and puncture-induced sepsis in rats
(Springer, 2025) Ozhan, Onural; Yildiz, Azibe; Balcioglu, Sevgi; Gunal, Selami; Vardi, Nigar; Ates, Burhan; Gunata, Mehmet
Background The purpose of this research was to determine how Rhizopogon luteolus extract (RLE) affects rat cecal ligation and puncture (CLP)-induced sepsis. Methods Wistaralbino rats were randomly assigned to six groups (n = 8). (1) Sham group; (2) CLP group; (3) CLP+1% RLE group; (4) CLP+5% RLE group; (5) CLP+10% RLE group; (6) CLP+25% RLE group. Rat liver, kidney, ileum, and lung tissues were examined histologically and biochemically. Results The degree of infiltration in the lung, liver, ileum, and renal tissues was substantially lower in the CLP+10% RLE group than in the CLP group. With the exception of the kidneys, all tissues in the CLP+25% RLE group showed substantial histological improvement. Compared with the CLP group, the CLP group presented considerable reductions in mucosal epithelial degeneration and desquamation. The increased malondialdehyde levels in the liver, kidney and ileum tissues of the sepsis-induced groups were significantly lower than those in the RLE-treated groups by 5% or more. The decreased glutathione and catalase activities in the ileum tissues of the CLP groups significantly increased in the RLE-treated groups by 5% and above. The decrease in superoxide dismutase levels in the liver and kidney tissues of the sepsis-induced injury group increased significantly in the groups with 5% RLE in the liver tissue and 1% RLE in the kidney tissue. Conclusion RLE, which has been proven by in vitro studies, has therapeutic effects on the lung, liver, kidney and ileum tissues of rats with sepsis caused by CLP. Among the doses tested, 10% RLE exhibited the most consistent and significant protective effects in reducing histopathological damage and restoring antioxidant balance in sepsis-affected tissues.
The in vitro toxicity analysis of titanium dioxide (TiO2) nanoparticles on kinematics and biochemical quality of rainbow trout sperm cells
(Elsevier Science Bv, 2018) Ozgur, Mustafa Erkan; Balcioglu, Sevgi; Ulu, Ahmet; Ozcan, Imren; Okumus, Fatih; Koytepe, Suleyman; Ates, Burhan
In recent years, titanium dioxide (TiO2) nanoparticles (NPs) as metal oxide nanoparticles are widely used in industry, agriculture, personal care products, cosmetics, sun protection and toothpaste, electronics, foodstuffs and food packaging. This use of nano-TiO2 has been associated with environmental toxicity concerns. Therefore, the aim of this study was to evaluate the in vitro effect of different doses of TiO2 NPs (similar to 30-40 nm) (0.01, 0.1, 0.5, 1, 10 and 50 mg/L) at 4oC for 3 h on the sperm cell kinematics as velocities of Rainbow trout (Oncorhynchus mykiss, Walbaum, 1792) sperm cells. Furthermore, oxidative stress markers (total glutathione (TGSH) and superoxide dismutase (SOD) were assessed in sperm cells after exposure to TiO2 NPs. According to the obtained results, there were statistically significant (P < 0.05) decreasing in the velocities of sperm cells after 10 mg/L TiO2 NPs and an increase the activity of SOD (P < 0.05) and TGSH levels were determined.
Investigation of toxic effects of amorphous SiO2 nanoparticles on motility and oxidative stress markers in rainbow trout sperm cells
(Springer Heidelberg, 2019) Ozgur, Mustafa Erkan; Ulu, Ahmet; Ozcan, Imren; Balcioglu, Sevgi; Ates, Burhan; Koytepe, Suleyman
In this study, we investigated the effects of SiO2 nanoparticles (SiO2-NPs) (1, 10, 25, 50, and 100mg/L) for 24h in vitro on the motility parameters and oxidative stress markers such as total glutathione (TGSH), catalase (CAT), and malondialdehyde (MDA) of rainbow trout, Oncorhynchus mykiss sperm cells. Therefore, SiO2-NPs were synthesized with sol-gel reaction from tetraethoxy orthosilicate (TEOS). The prepared nanoparticle structures were characterized for chemical structure, morphology and thermal behavior employing Fourier transform infrared spectroscopy, X-ray spectroscopy, scanning electron micrograph, and thermal analysis (DTA/TGA/DSC) techniques. After exposure, there was statistically significant (p<0.05) decreases in velocities of sperm cells. CAT activity significantly (p<0.05) decreased by 9.6% in sperm cell treated with 100mg/L. In addition, MDA level significantly increased by 70.4% and 77.5% in sperm cell treated with 50 and 100mg/L SiO2-NPs, respectively (p<0.05). These results showed that SiO2-NPs may have toxic effect on rainbow trout sperm cells in 50mg/L and more.
Melatonin protects sperm cells of Capoeta trutta from toxicity of titanium dioxide nanoparticles
(Springer Heidelberg, 2020) Ozgur, Mustafa Erkan; Ulu, Ahmet; Noma, Samir Abbas Ali; Ozcan, Imren; Balcioglu, Sevgi; Ates, Burhan; Koytepe, Suleyman
In this study, it was aimed to determine the protective effects of melatonin (0.01, 0.1, and 1 mM) against 10 mg/L titanium dioxide nanoparticles (TiO2-NPs) on kinematic and oxidative indices in the sperm cells of Capoeta trutta. Therefore, TiO2 nanoparticles were synthesized primarily within the scope of the study. The synthesized nanoparticles were characterized by structurally different techniques. Then, melatonin and TiO2 were applied to Capoeta trutta sperm cells by in vitro. According to our data, all doses of melatonin showed protective effects on all velocities of sperm cells such as the straight line velocity (VSL), the curvilinear velocity (VCL), and the angular path velocity (VAP) against TiO2-NPs, while 0.1 and 1 mM doses of melatonin improved the VSL value. Although TiO2-NPs increased total glutathione (tGSH), malondialdehyde (MDA) lipid peroxidation, and superoxide dismutase (SOD) compared to the control group, there were positive treatment effects for all doses of melatonin on antioxidant capacity of sperm cells. At the end of this research, it is suggested that over 0.1 mM dose of melatonin improves the velocity of sperm cells and it plays a protective role against the toxic effects of TiO2-NPs.
N-acetyl cysteine amide mitigates oxidative stress and apoptosis in a rat model of renal ischemia-reperfusion injury
(Nature Portfolio, 2026) Ozhan, Onural; Ekici, Cihan; Ates, Burhan; Yildiz, Azibe; Balcioglu, Sevgi; Vardi, Nigar; Parlakpinar, Hakan
Renal ischemia-reperfusion (IR) injury is a major cause of acute kidney injury, in which oxidative stress (OS) and apoptosis play central roles. N-acetyl cysteine amide (NACA), a lipophilic derivative of N-acetylcysteine, exhibits improved cellular penetration and antioxidant activity. This study investigated the renoprotective effects of NACA in a rat model of renal IR injury. Twenty-eight female Wistar albino rats were randomized into four groups (n = 7): Control, IR, NACA + IR (100 mg/kg i.p., 30 min before ischemia), and IR + NACA (100 mg/kg i.p., immediately after ischemia). Following right nephrectomy, the left renal pedicle was clamped for 60 min and reperfused for 24 h. Serum renal function markers, kidney OS parameters, histopathological injury, and caspase-3 immunoreactivity were evaluated. Renal IR injury significantly increased serum blood urea nitrogen and creatinine levels and induced histopathological damage characterized by tubular dilatation, cast formation, and degeneration. Catalase (CAT) and superoxide dismutase (SOD) activities were significantly altered; malondialdehyde increased after IR and was reduced by NACA pretreatment, whereas myeloperoxidase and total glutathione did not differ significantly among groups. NACA pretreatment attenuated inflammatory cell infiltration, tubular dilatation, and caspase-3 immunoreactivity, while partially restoring CAT and SOD activity. Post-ischemic NACA administration was less effective, particularly in reducing apoptosis and inflammatory infiltration. NACA confers partial renoprotection against renal IR injury, with pretreatment providing superior efficacy. These findings highlight the importance of antioxidant timing and suggest NACA as a potential prophylactic strategy when renal ischemia is predictable.

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