Yazar "Basak-Turkmen, Nese" seçeneğine göre listele

Listeleniyor 1 - 2 / 2
  • Küçük Resim Yok
    Öğe
    The Novel Synthesized Pyridazinone Derivates Had the Antiproliferative and Apoptotic Effects in SHSY5Y and HEP3B Cancer Cell Line
    (Bentham Science Publ Ltd, 2018) Ciftci, Osman; Ozdemir, Zeynep; Acar, Ceren; Sozen, Mert; Basak-Turkmen, Nese; Ayhan, Idris; Gozukara, Harika
    Background: Brain cancer (neuroblastoma) and liver cancer (hepatocellular carcinoma) are common cancer types among others worldwide which do not have a radical treatment and cure. Objective: In the current study, five novel pyridazinone derivates bearing benzelhydrazone moiety at second position were synthesized and evaluated for their cytotoxic activity against neuroblastoma and hepatocellular carcinoma (SHSY5Y and HEP3B) and human fibroblast (HF) cell lines. The aim of the current study is to identify antiproliferative activity of five novel pyridazinone derivates against neuroblastoma and hepatocellular carcinoma (SHSY5Y and HEP3B) and human fibroblast (HF) cell lines. Method: The compounds were synthesized by the reacting 6-[4-(phenyl/4-chlorophenyl)piperazine-1yl]-3(2H)-pyridazinone-2-yl acetohydrazide with benzaldehyde in ethanol. The in vitro antiproliferative activities were determined with MTT assay. Bax, Bcl-2 and Casp3 gene expression levels were detected with RT-PCR analyses. Results: The lowest IC50 was observed for compound 4 in SHSY5Y and HEP3B cells. Apoptosis increased in cancer cells which was shown by changes inBax, Bcl-2 and Casp3 gene expression levels with 1-5 compound therapy. Conclusion: Novel pyridazinone derivates might be promising agents as new chemotherapeutic candidates in brain and liver cancer.
  • Küçük Resim Yok
    Öğe
    SYNTHESIS OF NEW 6-[4-(2-FLUOROPHENYLPIPERAZINE-1-YL)]-3(2H)-PYRIDAZINONE-2-ACETHYL-2-(SUBSTITUTEDBENZAL)HYDRAZONE DERIVATIVES AND EVULATION OF THEIR CYTOTOXIC EFFECTS IN LIVER AND COLON CANCER CELL LINES
    (Springer, 2019) Ozdemir, Zeynep; Basak-Turkmen, Nese; Ayhan, Idris; Ciftci, Osman; Uysal, Mehtap
    In this study, seven new 3(2H)-pyridazinone derivatives expected to show cytotoxic activity in liver and colon cancer cell lines were synthesized. Their structures were confirmed by the IR, H-1-NMR, C-13-NMR spectra and elementary analyses. Compunds V-1-V-7 were tested on HEP3B (liver cancer) and HTC116 (colon cancer) cell lines for cytotoxicity by using MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium] proliferation assay. Human fibroblast cells were used as safety control in these tests. 6-[4-(2-Fluorophenyl)piperazine-1-yl]-3(2H)-pyridazinone-2-acetyl-2-(2-chlorobenzal) hydrazone (compound V-3) was the most active agent with respect to HEP3B and HTC116 cell lines.