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Öğe Antioxidant activity and luteolin content of Marchantia polymorpha L(Tubitak Scientific & Technological Research Council Turkey, 2012) Gokbulut, Alper; Satilmis, Basri; Batcioglu, Kadir; Cetin, Barbaros; Sarer, EnginAntioxidant activity of the methanol and ethyl acetate extracts of Marchantia polymorpha L., which grows naturally in Turkey, was tested by DPPH and ABTS methods. According to the applied methods, both extracts exhibited moderate activity. Some phenolic compounds that could be responsible for the activity were investigated by RP-HPLC, qualitatively and quantitatively, in the methanolic extract of liverwort. While caffeic acid, chlorogenic acid, rutin, myricetin, quercetin, and kaempferol were not found in the extract, luteolin as the liable antioxidant agent was determined as 0.0052 +/- 0.0002% among the investigated compounds.Öğe Antioxidant and Antimicrobial Activities, and Phenolic Compounds of Selected Inula species from Turkey(Sage Publications Inc, 2013) Gokbulut, Alper; Ozhan, Onural; Satilmis, Basri; Batcioglu, Kadir; Gunal, Selami; Sarer, EnginThree Inula species, I. viscosa, I. helenium ssp. turcoracemosa and I. montbretiana, collected from different locations of Anatolia were investigated for their antioxidant and antimicrobial potential, and their total phenolic content and phenolic composition. Antioxidant activities of various extracts of the plant parts were measured using DPPH radical scavenging and ABTS assays. Antimicrobial potential of methanol extracts of the plant parts was determined by the agar dilution method against Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Pseudomonas aeruginosa, Candida albicans and Candida tropicalis. All the extracts were more active against Gram-positive bacteria and yeasts than Gram-negative bacteria. The extracts exhibited antioxidant and antimicrobial activities in different concentrations. Total phenolic concentration of the extracts was estimated with Folin-Ciocalteu reagent using gallic acid as standard. The total phenolic content varied widely in different parts of the three tested Inula species, ranging from 21.1 +/- 0.8 to 190.9 +/- 6.1 mg GAE/g extract. Phenolic components, such as chlorogenic acid, caffeic acid, rutin, myricetin, quercetin, luteolin and kaempferol were quantified by HPLC-DAD in the methanol extracts of the Inula species. It was obvious that the antioxidant and antimicrobial properties of the plants were due to the phenolics.Öğe Antioxidant and antithrombotic properties of fruit, leaf, and seed extracts of the Halhal? olive (Olea europaea L.) native to the Hatay region in Turkey(Kemerovo State Univ, 2023) Batcioglu, Kadir; Kucukbay, Fatumetuzzehra; Alagoz, Mehmet Abdullah; Gunal, Selami; Yilmaztekin, YakupThe olive (Olea europaea L.) is one of the most important plants grown in many Mediterranean countries that has a high economic value. Olives, which are specific to each region, have different bioactive components. In this study, we investigated the phenolic/flavonoid contents, as well as antioxidant, antimicrobial, and antithrombotic activities of the fruit, leaf, and seed extracts obtained from the Halhali olive grown in Arsuz district of Hatay, Turkey. Antioxidant activities of the phenolic compounds found in the olive fruit, seed, and leaf extracts were determined by employing established in vitro systems. Total phenolics were determined as gallic acid equivalents, while total flavonoids were determined as quercetin equivalents. Also, we evaluated a possible interaction between oleuropein and aggregation-related glycoproteins of the platelet surface via docking studies. The extracts showed effective antioxidant activity. The seed extract had the highest phenolic content of 317.24 mu g GAE, while the fruit extract had the highest flavonoid content of 4.43 mu g. The highest potential for metal chelating activity was found in the leaf extract, with an IC50 value of 13.33 mg/mL. Also, the leaf extract showed higher levels of antioxidant, antithrombotic, and antimicrobial activity, compared to the fruit and seed extracts. The docking scores of oleuropein against the target molecules GPVI, alpha 2 beta 1, and GPIb alpha were calculated as -3.798, -4.315, and -6.464 kcal/mol, respectively. The olive fruit, leaf, and seed extracts used as experimental material in our study have remarkable antioxidant, antimicrobial, and antithrombotic potential.Öğe Antioxidative effect of melationin, ascorbic acid and N-acetylcysteine on caerulein-induced pancreatitis and associated liver injury in rats(Baishideng Publishing Group Inc, 2006) Esrefoglu, Mukaddes; Gul, Mehmet; Ates, Burhan; Batcioglu, Kadir; Selimoglu, Mukadder AyseAIM: To investigate the role of oxidative injury in pancreatitis-induced hepatic damage and the effect of antioxidant agents such as melatonin, ascorbic acid and N-acetyl cysteine on caerulein-incluced pancreatitis and associated liver injury in rats. METHODS: Thirty-eight female Wistar rats were used. Acute pancreatitis (AP) was induced by two i.p. injections of caerulein at 2-h intervals (at a total dose of 100 mu g/kg b.wt). The other two groups received additional melatonin (20 mg/kg b.wt) or an antioxidant mixture containing L(+)-ascorbic acid (14.3 mg/kb.wt.) and N-acetyl cysteine (181 mg/kg b.wt.) i.p. shortly before each injection of caerulein. The rats were sacrificed by decapitation 12 h after the last injection of caerulein. Pancreatic and hepatic oxidative stress markers were evaluated by changes in the amount of lipid peroxides measured as malondialdehyde (MDA) and changes in tissue antioxidant enzyme levels, catalase (CAT) and glutathione peroxidase (GPx). Histopathological examination was performed using scoring systems. RESULTS: The degree of hepatic cell degeneration, intracellular vacuolization, vascular congestion, sinusoidal dilatation and inflammatory infiltration showed a significant difference between caerulein and caerulein + melatonin (P=0.001), and careulein and caerulein+L(+)ascorbic acid +N-acetyl cysteine groups (P=0.002). The degree of aciner cell degeneration, pancreatic edema, intracellular vacuolization and inflammatory infiltration showed a significant difference between caerulein and caerulein+melatonin (P=0.004), and careulein and caerulein+L(+)-ascorbic acid+N-acetyl cysteine groups (P=0.002). Caerulein-induced pancreatic and liver damage was accompanied with a significant increase in tissue MDA levels (P= 0.01, P = 0.003, respectively) whereas a significant decrease in CAT (P=.0.002, P=0.003, respectively) and GPx activities (P=0.002, P=0.03, respectively). Melatonin and L(+)-ascorbic acid+N-acetyl cysteine administration significantly decreased MDA levels in pancreas (P=0.03, P=0.002, respectively) and liver (P=0.007, P=0.01, respectively). Administration of these agents increased pancreatic and hepatic CAT and GPx activities. Melatonin significantly increased pancreatic and hepatic CAT (P=0.002, P=0.001, respectively) and GPx activities (P=0.002, P=0.001). Additionally, L(+)-ascorbic acid+N-acetyl cysteine significantly increased pancreatic GPx (P = 0.002) and hepatic CAT and GPx activities (P= 0.001, P= 0.007, respectively) CONCLUSION: Oxidative injury plays an important role not only in the pathogenesis of AP but also in pancreatitis-induced hepatic damage. Antioxidant agents such as melatonin and ascorbic acid+N-acetyl cysteine, are capable of limiting pancreatic and hepatic damage produced during AP via restoring tissue antioxidant enzyme activities. (c) 2006 The WJG Press. All rights reserved.Öğe Evaluation of Oxidant Injury Induced by Irradiation in Brain Tissues of Rats of Different Ages(Aves Yayincilik, Ibrahim Kara, 2009) Uyumlu, Ayse Burcin; Erkal, Haldun Suekrue; Batcioglu, Kadir; Serin, Meltem; Yucel, NeslihanObjectives: We aimed to evaluate the age-related changes of oxidative injury in the brain tissues of rats produced by radiotherapy that is widely used on cancer treatment. Study Design: Fifty-five male Wistar albino rats [ages of rats were 1, 4, 12 weeks (n=10) and 1 year (n=5)] were divided into four groups. Irradiation were performed on a Cobalt-60 unit using a single fraction of 8 Gy. The brain tissues were homogenized and divided into two portions. One portion was used for the measurement of the malondialdehyde (MDA). The other portion was used for the measurement of the protein concentration, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSHPx) enzyme activities. Results: The SOD activity decreased significantly in 1-week-old rats (p<0.05). The decreases in GSHPx and CAT activities were more obvious in 1-week and 1-year-old rats than that in others. No significant changes were observed in adolescent and adult rats. The MDA levels of all groups increased. The highest MDA levels were seen in 1-year-old rats (p<0.05). Conclusion: Ionizing radiation used in radiotherapy affected antioxidant systems and increased MDA levels. These changes were more in the 1-week and 1-year-old rats than in others. This can be due to incomplete development of many systems in newborn rats and the loss of physiological capacities associated with aging in 1 -year-old rats.Öğe Hepatic Arginase - Nitric oxide imbalance: Impact of carcinogenesis and therapeutic effect of sodium channel blockage in an in vivo rat model(Walter De Gruyter Gmbh, 2016) Batcioglu, Kadir; Yildirim, Battal; Satilmis, Basri; Uyumlu, Ayse Burcin; Genc, Metin Fikret; Bentli, Recep; Djamgoz, Mustafa B. A.Objective: Nitric oxide synthase and arginase are frequently antagonistic and interactive, although both use L-arginine as common substrate. Their balance is of potential functional importance. How the balance changes in cancer is unknown. Increasing evidence suggests that progression of carcinomas involves functional voltage-gated sodium channel (VGSC) activity. Methods: The present study extended this study to liver and aimed to determine whether (i) DMBA carcinogenesis would affect the activities of arginase and NOS and (ii) treatment with Na-channel blocker RS100642 would ameliorate the impact of the carcinogen on the arginase-NOS balance. Results: DMBA application significantly increased arginase activity and, correspondingly, the level of L-ornithine by 25-33%. In contrast, NOS activity decreased by 11%. Importantly, RS100642 treatment completely suppressed the effect on arginase. Conclusion: It is concluded (i) that DMBA carcinogenesis changes the hepatic arginase-NOS balance, increasing the overall dominance of arginase and (ii) that VGSC inhibition has a protective effect on liver.Öğe Investigation of a weak magnetic field effect on the in vitro catalytic activity of adenosine deaminase and xanthine oxidase(General Physiol And Biophysics, 2011) Batcioglu, Kadir; Dogan, Metin; Uyumlu, Ayse B.; Satilmis, Basri; Bayri, Nevzat; Atalay, Selcuk; Demirtas, HakanThe effect of a weak magnetic field (MF) on adenosine deaminase (ADA) and xanthine oxidase (XOD) activities have been investigated. A 50 Hz uniform MF was generated, and the magnitude of the field was kept constant at 5.8 mT. The changes in ADA activity over time were significantly different in and out of the MF; MF caused a steeper decline in ADA activity compared to the situation when no MF is present. In addition, MF caused a significant increase in XOD activity. There were no significant time-related changes for either enzyme in the absence of the MF. We suggest that a weak MF affects enzymatic systems.Öğe Investigation of the Effect of Milrinone on Renal Damage in an Experimental Non-Heart Beating Donor Model(Taylor & Francis Inc, 2018) Uysal, Erdal; Dokur, Mehmet; Altinay, Serdar; Saygili, Eyup Ilker; Batcioglu, Kadir; Ceylan, Mehmet S.; Kazimoglu, HatemPurpose: In our study, it was aimed to investigate the preventive effect of milrinone on renal damage in experimental controlled non-heart-beating donors (NHBDs) model. Materials and Methods: Sixteen rats randomly divided into 2 groups, 8 rats in each were used. Group 1 was control, group 2 was milrinone group. Group 1 rats received 1.25ml 0.09% NaCl intraperitoneally equivalent to the milrinone diluted volume. Group 2 rats were administered intraperitoneally with 0.5mg/kg of milrinone 2hours before cardiac arrest. After the cardiac arrest, left nephrectomy was applied to the rats. Malondialdehyde, superoxide dismutase (SOD), catalase, glutathione peroxidase (GPx) activities, Caspase-3 (apoptotic index) and histopathological evaluation were performed in the tissues. Results: In the milrinone group, the total injury score was significantly lower relative to the control group (p = 0.001). Caspase-3 staining was moderately strong in the control group but weaker in the milrinone group. Apoptotic index was significantly lower in the milrinone group compared to the control group (p = 0.001). In comparison between groups, SOD and GPx in the milrinone group was significantly higher than the control group (p = 0.008, p = 0.006). Conclusions: Milrinone has been shown to be effective in the prevention of tissue damage due to oxidative stress and inflammatory process in the renal of warm ischemia in the experimental NHBDs model and in protecting the renal. Milrinone increases antioxidant activity while reducing apoptosis. Systemic administration of milrinone prior to cardiac arrest may be beneficial. Administration of milrinone to the recipient in the perioperative period may contribute to donor function.Öğe The investigation of the effect of NAD, H2O2 and weak magnetic field on the antibacterial mechanism of isoniazid (INH) that first line antibiotic against M. tuberculosis agent(2020) Gunal, Selami; Erdogan, Feyza Esra; Batcioglu, KadirAbstract: Tuberculosis is an infectious disease, which is caused by the Mycobacterium tuberculosis complex. This disease leads to up to 1.3 million deaths out of more than eight million cases every year. A prodrug called isoniazid has been proven to be effective and widely used in the treatment of infections caused by tuberculosis. Despite its use for more than six decades clinically, the action mechanism of this prodrug is yet to be elucidated. INH action agains mycobacteria requires catalase?peroxidase (KatG) function, and IN-NAD adduct formation is catalyzed in vitro by M. tuberculosis KatG under a variety of conditions. Low-intensity EMF (Electromagnetic Field) has been used in therapeutic practices in addition to its use in telecommunication systems and food protection. EMF is used in medicine and food industries especially for its bacte-ricidal effects. In this study, we aimed to investigate the effects of weak magnetic field application and the addition of NAD and H2O2 on the action mechanism of isoniazid. We added H2O2 and NAD individually and together, to the different groups at varying concentrations. Also, one experimental group was exposed to a 5mT, 50Hz magnetic field for 4 to 5 hours per day (total of 45 hours in 10 days). The agar proportion method was used to evaluate the results. It was determined that the addition of 100 ?M NAD and H2O2 together increased the effectiveness of isoniazid to some extent. However, the application of a weak magnetic field did not change the effectiveness of the drug.Öğe Neuroprotective effects of the new Na channel blocker rs100642 in global ischemic brain injury(Termedia Publishing House Ltd, 2019) Kamisli, Suat; Basaran, Cenk; Batcioglu, Kadir; Oztanir, Mustafa Namik; Gul, Mehmet; Satilmis, Basri; Uyumlu, Ayse BurcinIntroduction: RS100642, a mexiletine analogue, is a novel sodium channel blocker with neuroprotective and antioxidant activities. The protectivity of RS100642, which has been shown against focal cerebral ischemia, was investigated in global cerebral ischemia in this study. Material and methods: Global cerebral ischemia was induced for five minutes in adult male Wistar Albino rats via the 4-vessel occlusion method. Intravenous administration of 1 mg/kg RS100642 following reperfusion for 30 min (RS100642 group) was compared with a sham treatment group (ischemia group) and nonischemized group (control) histologically based on morphology and caspase-3 immunohistochemistry, and biochemically based both on measurement of oxidative stress including malondialdehyde (MDA) levels, superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) activities and on assessment of apoptosis including caspase-3 and -8 activities and tumor necrosis factor alpha (TNF-alpha) levels at the end of 6 h. Results: While the RS100642 group had significantly lower MDA levels and higher SOD activities than the sham treatment group (p < 0.05), GPx and CAT activities of the RS100642 and sham treatment groups were similar (p > 0.05) and significantly lower than those of the controls (p < 0.05). Necrosis and caspase-3 activity and immunoreactivity in the RS100642 group were significantly lower than those in the sham treatment group (p < 0.05), while there was no significant difference between groups regarding caspase-8 and TNF-alpha (p > 0.05). Conclusions: Na+ channel blockade by RS100642 has remarkable neuroprotective effects following global brain ischemia/reperfusion damage. Further research is required to determine the optimum dose and time of administration.Öğe Oxidative Stress in the in vivo DMBA Rat Model of Breast Cancer: Suppression by a Voltage-gated Sodium Channel Inhibitor (RS100642)(Wiley, 2012) Batcioglu, Kadir; Uyumlu, A. Burcin; Satilmis, Basri; Yildirim, Battal; Yucel, Neslihan; Demirtas, Hakan; Onkal, RustemBreast cancer (BCa) was induced in vivo in female rats with 7,12-dimethylbenz(a)anthracene (DMBA). Two main questions were addressed. Firstly, would the carcinogenesis be accompanied by oxidative stress as signalled by superoxide dismutase, glutathione peroxidase, malondialdehyde and total nitrate? Secondly, would treating the rats additionally with a blocker of voltage-gated sodium channel (VGSC) activity, shown previously to promote BCa progression, affect the oxidative responses? The DMBA-induced increases in the antioxidant systems were completely blocked by the VGSC inhibitor RS100642, which also significantly prolonged the lifespan. We conclude that VGSC inhibition in vivo can significantly protect against oxidative stress and improve survival from tumour burden.Öğe Protective effect of Lycium barbarum on renal injury induced by acute pancreatitis in rats(Wolters Kluwer Medknow Publications, 2022) Dogan, Tugba; Batcioglu, Kadir; Kustepe, Elif Kayhan; Uyumlu, Ayse Burcin; Yilmaztekin, YakupBackground: Lycium barbarum (LB) is a plant species that is well known in Chinese traditional medicine and is also considered a nutrient, belonging to the Solanaceae family, also called goji berry or wolfberry. Objectives: The aim of this study was to investigate the protective efficacy of LB, in kidney damage caused by acute pancreatitis (AP). Materials and Methods: In the study, we used 36 female Wistar albino rats (12 in each group) which were divided into three groups: Control, cerulein (100 mu g/kg b. wt. intraperitonally) and Cerulein + LB (6 mg/ml/day gastric gavage) group. Serum lipase, Interleukin (IL)-1, and IL-6 levels were measured. Superoxide dismutase, catalase (CAT), glutathione peroxidase enzyme activity assays and 8-hydroxy deoxyguanosine, malondialdehyde (MDA), and protein levels were measured in kidney tissue samples. In addition, histopathological analysis was performed in kidney tissue samples. Results: According to the findings, in the AP model created with Cerulein, administration of LB plant extract decreased oxidative stress and damage caused by AP in the kidney tissue and partially suppressed the inflammatory reactions in the tissue. Conclusion: According to the findings, in the AP model created with Cerulein, administration of LB extract decreased oxidative stress and in kidney damage caused by AP.Öğe Targeting the PANoptosome with 3,4-Methylenedioxy-?-Nitrostyrene, Reduces PANoptosis and Protects the Kidney against Renal Ischemia-Reperfusion Injury(Taylor & Francis Inc, 2022) Uysal, Erdal; Dokur, Mehmet; Kucukdurmaz, Faruk; Altinay, Serdar; Polat, Sait; Batcioglu, Kadir; Sezgin, EfeObjectives: The objectives of this study were a) to investigate the effect of targeting the PANoptosome with 3,4-methylenedioxy-beta-nitrostyrene (MNS) on PANoptosis in the Renal ischemia-reperfussion (RIR) model b) to investigate the kidney protective effect of MNS toward RIR injury. Methods: Thirty-two rats were divided into four groups randomly. The groups were assigned as Control, Sham, DMSO (dimethyl sulfoxide) and MNS groups. The rats in the MNS group were intraperitoneally given 20 mg/kg of MNS 30 minutes before reperfusion. 2% DMSO solvent that dissolves MNS were given to the rats in DMSO group. Left nephrectomy was performed on the rats under anesthesia at the 6th hour after reperfusion. Glutathione peroxidase (GPx), malondialdehyde (MDA), catalase (CAT), superoxide dismutase (SOD) and 8-Okso-2'-deoksiguanozin (8-OHdG) levels were measured. Immunohistochemical analysis, electron microscopic and histological examinations were carried out in the tissues. Results: Total tubular injury score was lower in the MNS group (p < 0.001). Caspase-3, Gasdermin D and MLK (Mixed Lineage Kinase Domain Like Pseudokinase) expressions were considerably decreased in the MNS group (p < 0.001). Apoptotic index (AI) was found to be low in the MNS group (p < 0.001). CAT and SOD levels were higher in the MNS Group (p = 0.006, p = 0.0004, respectively). GPx, MDA, and 8-OH-dG levels were similar (p > 0.05) in all groups. MNS considerably improved the tissue structure, based on the electron microscopic analysis. Conclusions: Our results suggested that MNS administrated before the reperfusion reduces pyroptosis, apoptosis and necroptosis. These findings suggest that MNS significantly protects the kidney against RIR injury by reducing PANoptosis as a result of specific inhibition of Nod-like receptor pyrin domain-containing 3 (NLRP 3), one of the PANoptosome proteins.Öğe Testosterone levels of children with a diagnosis of developmental stuttering(Dove Medical Press Ltd, 2015) Selcuk, Engin Burak; Erbay, Lale Gonenir; Ozcan, Ozlem Ozel; Kartalci, Sukru; Batcioglu, KadirBackground: Stuttering is defined as a disruption in the rhythm of speech and language articulation, where the subject knows what he/she wants to say, but is unable to utter the intended word or phrase fluently. The effect of sex on development and chronicity of stuttering is well known; it is more common and chronic in males. We aimed to investigate the relationship between developmental stuttering and serum testosterone levels in this study. Materials and methods: In this study, we evaluated a total of 50 children (7-12 years of age); eight (16%) were female and 42 (84%) were male. Twenty-five children who stutter and 25 typically fluent peers with the same demographic properties (ages between 7 years and 12 years) were included in this study. The testosterone levels of the two groups were determined in terms of nanogram per milliliter (ng/mL) by enzyme-linked immunosorbent assay. The difference between the means of the two groups was analyzed. Results: The medians of the testosterone levels of the stutterer and control groups were determined as 20 ng/mL (range =12-184 ng/mL) and 5 ng/mL (range =2-30 ng/mL), respectively. Testosterone levels of the stutterer group were significantly higher than in the control group (P=0.001). Besides, there was a significant correlation between the severity of the stuttering and testosterone levels in the stutterer group (P=0.0001). Conclusion: The findings of this study show that testosterone may have an effect on the severity of developmental stuttering and on the clinical differences between sexes. However, further investigations are needed to show that testosterone may play a role in the etiology of developmental stuttering.
