Yazar "Bayram, Dilek" seçeneğine göre listele
Listeleniyor 1 - 5 / 5
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe Effect of Glyphosate on Some Protective Systems in Zea mays L.(Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi, 2018) Akbulut, Gülçin Beker; Yiğit, Emel; Bayram, DilekÖz: Glyphosate is an herbicide that is applied after non-selective germination and affects plant growth. In this study, glyphosate was applied to Zea mays L. after germination, at a concentration range from 0.017 to 0.145 M in a growth chamber. The effects of this herbicide on some antioxidant enzymes, lipid peroxidation, total chlorophyll and total carbohydrate content were investigated on days 1, 5 and 10 following the application. Results showed that peroxidase (POD), ascorbate peroxidase (APX), superoxide dismutase (SOD), catalase (CAT) and glutathione-S-transferase (GST) were increased in the groups treated with glyphosate on days 5 and 10, but the activities of reduced glutathione (GSH) and glutathione reductase (GR) were decreased on day 10. Malondialdehyde (MDA) content indicating lipid peroxidation was increased on days 1, 5 and 10. Changes in total chlorophyll and total carbohydrate contents were found to be time-dependent. These increases and decreases in the antioxidant activities, MDA content, total chlorophyll and total carbohydrate content in Zea mays L. were determined as a symptom of cytotoxicity caused due to glyphosate.Öğe EFFECT OF SALICYLIC ACID AND SELENIUM ON ANTIOXIDANT SYSTEM OF AVENA SATIVA L. UNDER FENOXAPROP-P-ETHYL STRESS(Parlar Scientific Publications (P S P), 2016) Yigit, Emel; Akbulut, Gulcin Beker; Bayram, Dilek; Kaya, Armagan; Gok, YetkinThe present study focuses on understanding the role of different antioxidants in plant defense against oxidative stress caused by fenoxaprop-p-ethyl herbicide. Besides ameliorative effect of exogenously applied salicylic acid (SA) and selenium (Se) on fenoxaprop-p-ethyl-induced toxicity was examined in Avena sativa L. It was determined that the MDA (malondialdehyde) content in the groups to which only herbicide was applied was higher than that of the groups to which SA and Se applied together with the herbicide. In plants exposed to fenoxaprop-p-ethyl, the activities of peroxidase (POD), ascorbate peroxidase (APX), superoxide dismutase (SOD), glutathione S-transferase (GST), glutathione reductase (GR) and content of glutathione (GSH) increased in the treatment groups compared to the control groups. The antioxidant activity increased in the control and treatment groups that were pre-treated with SA and Se compared to the groups that were not pre-treated with SA and Se. While catalase activity (CAT) decreased in SA pre-treated plants, it increased in Se pre-treated plants. These results indicate that the fenoxaprop-p-ethyl induces various plant defense responses in target plants and that treatment with exogenous SA and Se can increase stress resistance by altering these responses.Öğe THE EFFECTS OF ORGANIC SELENIUM ON SOME PHYSIOLOGICAL AND BIOCHEMICAL PARAMETERS IN HORDEUM VULGARE L. AND TRITICUM AESTIVUM L. EXPOSED TO SALT STRESS(Parlar Scientific Publications (P S P), 2012) Yigit, Emel; Akbulut, Gulcin Beker; Gok, Yetkin; Bayram, DilekIn this study, the protective role of organic selenium against salt stress on Hordeum vulgare L. and Triticum aestivum L. were determined. Seeds, treated with 0.004 M organic selenium in the pre-application, were germinated in environments exposed to 50, 100 and 200 mM salt stress (NaCl), in comparison to seeds without selenium treatment. The effects of 0.004 M organic selenium on peroxidase, ascorbate peroxidase, and lipid peroxidation were investigated in both plants. An evaluation of their germination and enzyme activities showed that organic selenium resulted in salient improvement when compared to the plants directly exposed to salt stress. The present study suggests that selenium can play a protective role during salt stress by enhancing the antioxidant defense system.Öğe Quızalofop-p-ethyl uygulanan Helianthus annuus L. bitkisinde salisilik asidin bazı biyokimyasal parametreler üzerine etkisi(İnönü Üniversitesi, 2011) Bayram, DilekBu çalışmada, Helianthus annuus L. üzerine quizalofop-p-etil herbisidi çimlenme sonrası 0.3-3.1 mM konsantrasyon aralığında uygulanmıştır. Bu herbisitin peroksidaz, askorbat peroksidaz, lipid peroksidasyonu, fotosentetik pigment sistemi ve toplam fenolik içeriği üzerindeki etkileri 1., 5., 10. ve 15. günlerde araştırıldı. Ayrıca bitkiye çimlenme öncesi 0.5 mM salisilik asit (SA) ve çimlenme sonrası quizalofop-p-etil herbisidi 0.3-3.1 mM aralığında uygulandı. bu gruplarda uygulamayı takiben bu herbisitin peroksidaz, askorbat peroksidaz, lipid peroksidasyonu, fotosentetik pigment sistemi, ve toplam fenolik içeriği üzerindeki etkileri 1., 5., 10. ve 15. günlerde araştırıldı. Çimlenme sonrası quizalofop-p-etil uygulanan gruplarda peroksidaz aktivitesi 1.,5., 10. ve 15. günlerde artış gösterdi. Genelde peroksidaz aktivitesi, SA ve quizalofop-p-etil uygulanan gruplarda 1., 5., 10. ve 15. günlerde artış gösterdi. Askorbat peroksidaz aktivitesi 5. günde artış gösterirken 10. ve 15. günde azalış gösterdi. Çimlenme öncesi 0.5 mM SA ve çimlenme sonrası quizalofop-p-etil uygulanan gruplarda askorbat peroksidaz aktivitesi genel olarak 15. güne kadar artış gösterdi. Çimlenme sonrası quizalofop-p-etil uygulanan gruplarda, malondialdehid (MDA) içeriği genel olarak 1., 5. ve 10. ve 15. günlerde 1.6-3.1 mM uygulama yapılan gruplar dışında artış gösterdi. Çimlenme öncesi 0.5 mM SA ve çimlenme sonrası quizallofop-p-etil uygulanan gruplarda MDA içeriği çimlenme sonrası quizalofop-p-petil uygulanan gruplara göre artış gösterdi. Çimlenme sonrası quizalofop-p-etil ile çimlenme öncesi 0.5 mM SA ve çimlenme sonrası quizalofop-p-etil uygulanan H. annuus'da klorofil a, klorofil b, karotenoid ve toplam klorofil ve toplam fenolik içeriğinin de önemli ölçüde değişimler saptandı. Ayçiçeğinde MDA seviyesi, pigment içeriği ve antioksidan aktivitesinde artış ve azalışın olması quizalofop-p-etilin toksisitesinden dolayı bir sitotoksisite semptomu olarak saptandı. Bulgularımız quizalofop-p-etilin ayçiçeğinde oksidatif strese neden olduğunu göstermektedir.Öğe Thymoquinone reduced RIPK1-dependent apoptosis caused by valproic acid in rat brain(2021) Tastemir Korkmaz, Deniz; Azirak, Sebile; Bilgic, Sedat; Bayram, Dilek; Ozer, Mehmet KayaAim: Valproic acid (VPA) is a commonly used antiepileptic drug and known to have a neurotoxic effect, but its mechanism is not yet understood. In the present study, we aimed to determine how the VPA causes cell death in the brain and to evaluate the protective effects of thymoquinone (TQ) on VPA-induced brain damage. Materials and Methods: Male Sprague–Dawley albino rats were divided into three groups: control, VPA (500 mg/kg/day) and VPA + TQ (500 mg/kg/day + 50 mg/kg/day) with seven rats in. At the end of the experiment, rats were sacrificed and brain samples were taken to measure the expression levels of Receptor-interacting serine/threonine-protein kinase-1 (RIPK1) and -3 (RIPK3) genes by quantitative real-time PCR (qRT-PCR), NADPH oxidase-4 (NOX4) and, caspase-3 (CAS-3) expression by immunohistochemistry and the structural changes in the brain tissue by histologically. Results: RIPK1 gene expression levels were significantly increased in the VPA group compared to the controls (p<0.05) and a decrease in VPA + TQ group against the VPA group. Also, NOX-4 and CAS-3 production were increased in the VPA group compared to the control group (p<0.05), and there is a markedly decrease in the VPA + TQ group compared to the VPA group. Conclusion: VPA induced RIPK1-dependent apoptosis, leading to cell deaths in the brain and TQ reduced its effects. Therefore, TQ uptake can be a supportive treatment method for long-term and high-dose VPA users to eliminate undesirable effects.
