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    Bacterial colonization in uninfected pilonidal cyst and its role in developing recurrence in postoperative period: Prospective study
    (2019) Ureyen, Orhan; Tekeli, Mehmet Tahsin; Ustuner, Mehmet Akif; Gungor, Hilmi; Bayram, Emine Deniz
    Aim: To determine the bacterial prevalence and its susceptibility to antibiotics, and additionally, to investigate the relationship between bacterial status and recurrence and complications with regard to whether bacteria reproduce in the cyst cavity in uninfected pilonidal cyst. Material and Methods: The cases included in the study were those who applied to our clinic due to pilonidal sinus and surgical operation was decided. Before the operation, culture specimen was taken using a sterile swab stick from the cyst hole. The cyst was excised with a safety margin. The excised material was opened under the guidance of the cyst hole and the cavity was entered and samples of cyst tissue and content were collected for culture-antibiogram. Results: Totally 46 cases were included in our study, Average age was 26 (18-47) years. Limberg flap was performed to 37 (78%) of the cases, and primary repair to 9 (22%) cases. Average follow-up period was 27.1 (sd±7.6) months. Normal bacterial skin flora was determined in 6 (13%) cases and significant bacterial reproduction in 4 (8.6%) cases (Enterococcus faecalis, Streptococcus anginosus, MRSA, Streptococcus dysgalactiae ) in the cyst cavity culture. In tissue culture however, skin bacterial flora was observed in 9 (19.5%) cases, while bacterial growth only in 1 case. Recurrence was determined in 3 (3.6%) cases in total. Bacterial growth, and other factors were not associated with recurrence (p> 0.05) Conclusion: In this study, non-infected pilonidal sinus surgery does not require bacterial culture to prevent recurrence
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    Pseudo-Outbreak of Stenotrophomonas maltophilia Isolated from Kidney Stones and Attributed to Inadequate Sterilization: Investigation of Molecular Typing and Clonal Relationship
    (2020) Yiş, Reyhan; Tosun, Selma; Bayram, Emine Deniz; Değirmenci, Tansu; Günlüsoy, Bülent; Otlu, Barış; Kuzucu, Çiğdem
    Abstract: Stenotrophomonas maltophilia is a gram-negative bacterium. Hospitals can be a source of S. maltophilia because it adheres to nonliving surfaces and forms a biofilm. This study was performed to investigate the clonal relationship between S. maltophilia isolates obtained from kidney stone samples. Samples of kidney stones taken from patients and surrogate samples from nephroscopes, cleaning solution, disinfectant solution were included in the study. The clonal relationship between isolates was determined by PFGE. S. maltophilia was isolated from 34 of 94 kidney stone samples sent from the urology operating room between July 2017 and January 2018. A total of 26 S. maltophilia strains (21 from kidney stone samples, three from nephroscopes, and two from urine culture) were isolated. PFGE showed that the 21 kidney stone isolates and the 3 S. maltophilia isolates obtained from the nephroscope belonged to the same clone. The two urine culture isolates showed no clonal relationship to the outbreak isolates and were considered sporadic. Molecular typing confirmed that this pseudo-outbreak was attributed to inadequate disinfection of the nephroscopes. After disinfection protocols were reviewed and revised as needed, especially regarding the removal of organic material from nephroscopes after use, no further bacterial growth was detected from kidney stone specimens obtained with nephroscopes