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    Green Synthesis, Characterization, Antioxidant, Antibacterial and Enzyme Inhibition Effects of Chestnut (Castanea sativa) Honey-Mediated Silver Nanoparticles
    (Mdpi, 2023) Keskin, Merve; Kaya, Gulsen; Bayram, Sinan; Kurek-Gorecka, Anna; Olczyk, Pawel
    In this study, chestnut honey-based silver nanoparticles (CH-AgNPs) were synthesized at different temperatures (30, 60 and 90 degrees C) and these nanoparticles were characterized by different techniques such as UV-vis spectrophotometer, Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and energy dispersive X-ray (EDX). The DPPH free radical scavenging assay was used to determine the antioxidant activity of the obtained nanoparticles. The inhibition effects of these nanoparticles for some clinically important enzymes such as myeloperoxidase and collagenase were investigated. In addition, the disk diffusion method (DDM), agar well diffusion (AWD), and minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) techniques were used to determine the antibacterial activity of CH-AgNPs. In honey-based silver nanoparticle production processes using green synthesis, it was determined that the nanoparticle sizes decreased from 55 to 27 nm with an increase in temperature. In addition, it was determined that the rate of inhibition of myeloperoxidase (36.4% to 34.0%) and collagenase enzymes (74.2% to 68.7%) increased with a decrease in particle size. As a result of the antibacterial activity tests, it was observed that CH-AgNPs have antibacterial activity against all target pathogens including Gram-positive and Gram-negative bacteria. The obtained results show that CH-AgNPs produced using chestnut honey have the potential to be used in fields such as medicine, pharmacy and cosmetic technology.
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    Palynological, chemical, antimicrobial, and enzyme inhibition properties of Cannabis sativa L. propolis
    (Springer, 2023) Ozkok, Asli; Karlidag, Semiramis; Keskin, Merve; Bayram, Sinan; Keskin, Saban; Karabulut, Ercan; Cicek, Fevzi
    In this study, both chemical characterization and biological activities of propolis samples obtained from Cannabis sativa L. (cannabis, hemp, marijuana) isolated colonies (research group) and obtained from colonies at a distance where the bee cannot fly to the isolation area (control group) were determined. Palynological analyses of propolis samples obtained from the research and control group colonies were made and the botanical origin of the cannabis plant and other plants in the research area and the plants in the control group were determined. Bioactive component analysis of propolis extracts was determined by the GC-MS technique. The inhibition effect of propolis samples on some enzymes of medical importance and their antimicrobial effects against many pathogenic microorganisms were also investigated. Palynological analyses showed that honey bees prefer cannabis plants (11.11%) when collecting propolis, and the dry matter percentage (94.6 +/- 1.8%), balsam (46.2 +/- 5.2%), and wax content (4.8 +/- 0.5%), total phenolic (26.44 +/- 1.08 mg GAE/mL) and total flavonoid (2.03 +/- 0.11 mg QE/mL) content and antioxidant capacity (88.16 +/- 2.27 mu M FeSO4 center dot 7H(2)O/mL(-1)) of the research group propolis samples containing hemp were found to be higher than the control group. Also, it was determined that the research group propolis sample contains cannabinoid derivatives (cannabidivarinic acid, 0.29%; delta (1)-tetrahydrocannabinolic acid, 0.59%; cannabidiolic acid, 0.48%), which are active compounds of the cannabis plant. Also, a high inhibition effect was detected on some enzymes of medical importance in propolis samples obtained from cannabis group colonies. In addition, the research group propolis samples also showed a high antimicrobial effect against many pathogenic microorganisms.