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    Carbontetrachloride induced acute liver damage and protective effect of n-acetylcysteine on rats with regenerated and non-regenerated liver
    (Walter De Gruyter Gmbh, 2016) Bilgic, Sedat; Ozerol, Elif; Iraz, Mustafa; Sahin, Nurhan; Tanbek, Kevser; Cigli, Ahmet
    Objective: Our aim was to investigate 70% partial hepatectomy (PH) groups, compare with not subjected to PH groups after exposure to hepatotoxic agents for alterations in the protective effects of antioxidant agents and sensitivity of the liver. Accordingly, we aimed to investigate the toxicity of a hepatotoxic agent, carbon tetrachloride (CCl4), and protective effects of an antioxidant, N-acetylcysteine (NAC), in experimental animal model. Methods: 67 male Wistar Albino rats were divided into 2 main groups to total 9 subgroups: group 1, underwent PH; group 2, not subjected to PH. 0.5 ml/kg CCl4 and 50 mg/kg NAC was given intraperitoneally (i.p.) to the groups. On postoperative day 9, 70% PH was performed according to the method of Higgins and Anderson. Finally, all rats were humanely killed. Results: Catalase (CAT) and superoxide dismutase (SOD) activities were significantly lower in both groups when CCl4 was administered. NAC treatment was found to significantly increase these parameters (P<0.05). Malondialdehyde (MDA) and protein carbonyl (PC) levels were significantly greater in both groups when CCl4 was administered (P<0.05). NAC treatment was found to significantly reduce these parameters. Conclusion: These results indicated that CCl4 increased oxidation products, reduced liver enzymatic activity and reduced proliferation activity in both hepatectomised and nonhepatectomised liver. The liver injury of CCl4 and the protective effect of NAC was similarly in both main groups. Consequently, making PH may not create a negative effect and an additional health problems in liver. Thus, these results can positively affect the decisions of the healthy liver donors.
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    Elevated cardiac oxidative stress in newborn rats from mothers treated with atosiban
    (Springer Heidelberg, 2012) Simsek, Yavuz; Celik, Onder; Karaer, Abdullah; Yilmaz, Ercan; Gul, Mehmet; Ozerol, Elif; Bilgic, Sedat
    The purpose of this study was to evaluate the cardiac and cerebral oxidative stress in the offspings of pregnant rats treated with oxytocin antagonist atosiban. Experimentally naive, adult female Wistar-albino rats (200-250 g) were mated with adult male rats for copulation. After confirming pregnancy, eight gravid rats were then randomly assigned into two equal groups. The animals were treated from days 15 to 20 of gestation. One group acted as a control group, and received intraperitoneal (i.p.) injections of saline in a daily dose volume of 6 mg/kg/day. The second group received 6 mg/kg/day i.p. atosiban. On day 21 of gestation, pups were delivered by cesarean. The heart and brain tissues of the newborn rats were dissected and sent for the measurement of total oxidant status, total antioxitant status and oxidative stress index. There was no significant difference in birthweight or in the number of pups between two groups. Newborns from atosiban-treated mothers showed significantly increased oxidative stress in the plasma and heart tissue than that of controls which was confirmed by histological examination (P < 0.05). Oxidative stress parameters and histopathological results of the brain tissues of newborns were similar between two groups (P > 0.05). Oxytocin receptor blockage for the treatment of premature delivery may be associated with increased fetal morbidity and mortality secondary to the elevated oxidative stress in the heart of the newborns.
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    Hepatoprotective effect of royal jelly, grape seed extract, and Lycium barbarum against diethylnitrosamine-induced liver toxicity in rats
    (2018) Bilgic, Sedat; Dogan, Zumrut; Azirak, Sebile; Erdemli, Mehmet Erman; Onderci, Muhittin; Turk, Ahmet; Ozer, Mehmet Kaya
    Aim: We aimed to investigate, the effects of royal jelly (RJ), grape seed extract (GSE), and Lycium barbarum extract (LBAE) against diethylnitrosamine (DEN) induced hepatotoxicity, in experimental animal model. Material and Methods: Fifty female Sprague Dawley rats were divided into five groups (n=10): Control, DEN, DEN+RJ, DEN+GSE, DEN+LBAE. DEN administrated groups were intraperitoneally (i.p.) injected with three separate administration of DEN (200 mg/kg), on the zero, fifteenth and thirtieth treatment day. Then 100 mg/kg of RJ was given to DEN+RJ group, 100 mg/kg of GSE was given to DEN+GSE group, and 400 mg/kg LBAE was given to DEN+LBAE group with the daily drinking water from day 0 for 16 weeks. Histopathologic alterations including apoptotic changes of liver were evaluated. Results: RJ, GSE, and LBAE treatments significantly reduced weight loss induced by DEN. DEN administrated rats significantly increases malondialdehyde (MDA) level. It also efficiently decreases glutathione (GSH) level and catalase (CAT), superoxide dismutase (SOD) activity. These results were significantly ameliorated by dietary supplements (p<0.05). In addition, they increased the total antioxidant status (TAS) level and decreased serum oxidative stress index (OSI), total oxidant status (TOS), alanine aminotransferase (ALT), aspartate aminotransferase (AST), and gamma glutamyl transpeptidase (GGT) levels significantly (p<0.05). TUNEL positive cells were extremly pervasive in the livers of DEN group. Conclusion: Improvements were prominent in case of RJ > GSE > LBAE. Our results indicated that RJ, GSE and LBAE might be useful for prevention of hepatotoxicity induced by DEN via ameliorative effects on biochemical and oxidative stress indices
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    Olanzapine-induced renal damages and metabolic side effects: the protective effects of thymoquinone
    (2018) Bilgic, Sedat; Tastemir Korkmaz, Deniz; Azirak, Sebile; Guvenc, Ayse Nilay; Kocaman, Nevin; Ozer, Mehmet Kaya
    Aim: The goal of the study is to examine the protective qualities of thymoquinone (TQ) against the side-effects of olanzapine (OLZ) in an experimental model in rat kidneys. Material and Methods: Thirty five female Spraque-Dawley rats were divided into 5 groups (n=7): Control, OLZ, OLZ+TQ-1, OLZ+TQ-2, OLZ+TQ-3. All treatments were administered for two weeks by gavage. Two weeks administration of OLZ (4 mg/kg, once a day for the first week, 8 mg/kg once a day for the second week, p.o.) was given to all groups, except control. TQ was administered (25, 50, 100 mg/kg, once daily) by gastric tube. On treatment day 15, kidney tissues were removed for analysis. Results: TQ increased the total antioxidant status (TAS) and decreased creatinine (Cr), blood urea nitrogen (BUN), oxidative stress index (OSI) and total oxidant status (TOS) levels significantly (p<0.05). Conclusion: These results revealed that TQ improved the side-effects of OLZ, contributed to the oxygen radical scavenging activity, increased antioxidant activity and had ameliorative effects on recovery of increased serum biochemical and oxidative stress parameters. Thus, these results demonstrated that TQ had protective and antioxidant effects against adverse effects of OLZ in kidney of rats. TQ could be an effective course of therapy to enhance therapeutic efficacy.
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    The protective effect of erdosteine on short-term global brain ischemia/reperfusion injury in rats
    (Pergamon-Elsevier Science Ltd, 2009) Ozerol, Elif; Bilgic, Sedat; Iraz, Mustafa; Cigli, Ahmet; Ilhan, Atilla; Akyol, Omer
    Experimental studies have demonstrated that free radicals play a major role on neuronal injury during ischemia/reperfusion (I/R) in rats. Erdosteine is a thioderivative endowed with mucokinetic, mucolytic and free-radical-scavenging properties. The aim of the present study was to investigate the effect of erdosteine treatment against short-term global brain ischemia/reperfusion injury in rats. The study was carried out on Wistar rats divided into four groups. (i) Control group, (ii) ischemia/reperfusion group, (iii) ischemia/reperfusion + erdosteine group, and (iv) erdosteine group. Superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) activities as well as thiobarbituric acid reactive substances (TBARSs) and nitric oxide (NO) levels were analysed in erythrocyte and plasma of rats. Plasma NO levels were significantly higher in the ischemia/reperfusion group than the other groups. The activities of SOD and GSH-Px were decreased, while TBARS levels increased in the ischemia/reperfusion group compared to other groups in both plasma and erythrocyte. The erythrocyte CAT activity was higher in erdosteine group and there was a statistically significant increase, when compared with the erdosteine plus ischemia/reperfusion group. By treating the rats with erdosteine, the depletion of endogenous antioxidant enzymes (SOD, CAT, GSH-Px) and increase of TBARS and NO levels were prevented. This study, therefore, suggests that erdosteine reduces parameters of oxidative stress is well supported by the data. (c) 2008 Elsevier Inc. All rights reserved.
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    Thymoquinone reduced RIPK1-dependent apoptosis caused by valproic acid in rat brain
    (2021) Tastemir Korkmaz, Deniz; Azirak, Sebile; Bilgic, Sedat; Bayram, Dilek; Ozer, Mehmet Kaya
    Aim: Valproic acid (VPA) is a commonly used antiepileptic drug and known to have a neurotoxic effect, but its mechanism is not yet understood. In the present study, we aimed to determine how the VPA causes cell death in the brain and to evaluate the protective effects of thymoquinone (TQ) on VPA-induced brain damage. Materials and Methods: Male Sprague–Dawley albino rats were divided into three groups: control, VPA (500 mg/kg/day) and VPA + TQ (500 mg/kg/day + 50 mg/kg/day) with seven rats in. At the end of the experiment, rats were sacrificed and brain samples were taken to measure the expression levels of Receptor-interacting serine/threonine-protein kinase-1 (RIPK1) and -3 (RIPK3) genes by quantitative real-time PCR (qRT-PCR), NADPH oxidase-4 (NOX4) and, caspase-3 (CAS-3) expression by immunohistochemistry and the structural changes in the brain tissue by histologically. Results: RIPK1 gene expression levels were significantly increased in the VPA group compared to the controls (p<0.05) and a decrease in VPA + TQ group against the VPA group. Also, NOX-4 and CAS-3 production were increased in the VPA group compared to the control group (p<0.05), and there is a markedly decrease in the VPA + TQ group compared to the VPA group. Conclusion: VPA induced RIPK1-dependent apoptosis, leading to cell deaths in the brain and TQ reduced its effects. Therefore, TQ uptake can be a supportive treatment method for long-term and high-dose VPA users to eliminate undesirable effects.