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    Enhanced production of laccase by fungi under solid substrate fermentation condition
    (2011) Boran F.; Yeşilada O.
    Funalia trogii and Trametes versicolor were grown on agro byproduct wheat bran moistened with various natural moistening agents, and their effects on laccase production under solid substrate condition were investigated. Laccase was the main enzyme detected under this condition. High levels of laccase activity were obtained with solid substrate cultures moistened with olive oil mill wastewater (OOMW) or alcohol factory wastewater (vinasse). Among the cultures without inducer, T. versicolor culture was detected as a more effective laccase producer than F. trogii culture. Copper and xylidine were used as laccase inducers, and copper induced laccase production more than xylidine. The maximum laccase activity was detected as 14.18 U/mL with F. trogii grown on wheat bran moistened with 5 mM CuSO 4.5H 2O added 25% vinasse. Azo dye decolorization activity of the supernatants from solid substrate cultures was also tested. While the use of 0.063 U/mL F. trogii laccase in reaction solution gave 66% decolorization in a minute, it was 14% for T. versicolor. This method can be a possible alternative for valorization of lignocellulosic materials and industrial wastewaters during solid substrate fermentation and for obtaining enzyme source with very high decolorization activity.
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    Laccase Production of Newly Isolated Trametes versicolor under Batch, Repeated-Batch, and Solid-State Fermentation Processes
    (ABADER (Adıyaman Bilimsel Arastırmalar Dernegi), 2022) Tutal T.; Yeşilada Ö.; Boran F.
    In this study, the laccase production ability of the newly isolated Trametes versicolor strain was investigated in three different fermentation processes. In all three fermentation processes, the fungus was able to produce the laccase enzyme. During the solid-state fermentation process 13.21 U/mL laccase activity was detected on the 20th day in the 10 mM copper-containing medium, while this value reached to 27.30 U/mL in the medium containing 0.5 mM ABTS+10 mM copper. During the liquid batch fermentation process, laccase activity was significantly induced in the medium containing 1 mM copper and the laccase activities reached 2.25, 19.83 and 24.57 U/mL compared to the medium without copper on the 3rd, 6th, and 9th days, respectively. ABTS and xylidine induced the laccase production of this strain at a much lower level than copper. The liquid repeated-batch process also significantly induced the laccase production. While low level of enzyme activities were detected in a copper-free medium, laccase activities were induced in the copper-containing medium and the activity increased from 0.66 U/mL to 9.87 U/mL at the 6th use of the pellets. Copper was detected as an effective inducer for laccase production in all fermentation processes and activity staining after native polyacrylamide gel electrophoresis clearly showed the active laccase bands. The results revealed that this strain is a good laccase producer and the laccase production yield varies depending on the fermentation process, production time, and inducer used. © 2022, ABADER (Adıyaman Bilimsel Arastırmalar Dernegi). All rights reserved.