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    Laccase production and dye decolorization by Trametes versicolor: application of Taguchi and Box-Behnken Methodologies
    (Turkish Biochem Soc, 2014) Gedikli, Serap; Aytar, Pinar; Buruk, Yeliz; Apohan, Elif; Cabuk, Ahmet; Yesilada, Ozfer; Burnak, Nimetullah
    Objective: The aim of this study was to investigate the laccase production of Trametes versicolor under submerged fermentation condition. Then, dye decolorization by laccase was optimized using Box-Behnken methodology. Methods: The optimal culture conditions for producing high amount of laccase were determined using Taguchi methodology. The experiments were designed with five factors (glucose, yeast extract, CuSO4, inoculum size and pH) at three levels with orthogonal array layout of L27 (3(5)). Then, the optimum conditions for high decolorization activity of Reactive Blue 49 by obtained crude laccase were also investigated using Box-Behnken methodology. Results: The optimum culture conditions for production of high amounts of laccase were detected as 2 g L-1 of glucose, 5 g L-1 of yeast extract, 2mM of CuSO4, 4% of inoculum amount and pH 5.5. Yeast extract was the most effective factor, followed by CuSO4, inoculum, glucose and pH. Under these conditions, predicted values were in a good agreement with the actual experimental one. The predicted results showed that the maximum of Reactive Blue 49 decolorization as 98% could be obtained under the optimum conditions of pH 2.95, initial dye concentration 55.6 mg L-1, enzyme amount 0.76 mL and reaction time 46.91 min. The validity and practicability of this statistical optimization strategy was confirmed with the relation between predicted and experimental values. Conclusion: The results suggested that Taguchi method can be used in the optimization of laccase production process. Production of laccase by Trametes versicolor 2008001 can be effectively used for enzymatic decolorization according to the results of decolorization experiments in optimal levels.