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Öğe ACA, an inhibitor phospholipases A2 and transient receptor potential melastatin-2 channels, attenuates okadaic acid induced neurodegeneration in rats(Pergamon-Elsevier Science Ltd, 2017) Cakir, Murat; Duzova, Halil; Tekin, Suat; Taslidere, Elif; Kaya, Gul Busra; Cigremis, Yilmaz; Ozgocer, TubaAim: In recent studies, it has been shown that the Transient Receptor Potential Melastatin-2 Channels (TRPM2) and Phospholipases A2 (PLA(2)) inhibitors may have a protective effect on neurons. This study was aimed to investigate the protective effect of TRPM2 and PLA(2) inhibitor N-(p-amylcinnamoyl) Anthranilic Acid (ACA) in a neurodegenerative model induced by Okadaic Acid (OKA). Main methods: OKA (200 ng/10 mu l) was administered bilateral intracerebroventricularly as a single injection. Key findings: OKA-treated rats showed significant impairments of spatial memory in Morris Water Maze Test. OKA-induced memory-impaired rats showed increased numbers of degenerated neurons and Caspase-3, tau phosphorylated ser396, beta-amyloid positive cells in the hippocampus and cerebral cortex. Furthermore, OKA-treated rats exhibited significantly increased MDA, TNF-alpha levels, and decreased SOD, GSH-PX enzyme activates and GSH levels of the tissues. ACA administration ameliorated OKA-induced memory impairment in rats. The ACA treatment also increased SOD and GSH-PX enzyme activation and GSH levels, and conversely decreased the levels of MDA, TNF-alpha. It was found that the numbers of the degenerated neurons and Caspase-3 positive cells of cortex and hippocampus regions were significantly reduced. Significance: ACA administration attenuates the oxidative stress and neuroinflammation of OKA-induced neurodegeneration; and ameliorates the cognitive decline and neurodegeneration. (C) 2017 Elsevier Inc. All rights reserved.Öğe The ameliorating effect of cannabinoid type 2 receptor activation on brain, lung, liver and heart damage in cecal ligation and puncture-induced sepsis model in rats(Elsevier, 2020) Cakir, Murat; Tekin, Suat; Okan, Asli; Cakan, Pinar; Doganyigit, ZuleyhaUncontrolled infection and increased inflammatory mediators might cause systemic inflammatory response. It is already known that Cannabinoid Type 2 (CB2) receptors, which are commonly expressed in immune cells and in many other tissues, have an effect on the regulation of immune response. In the present study of ours, the effects of CB2 receptor agonist JWH-133 was investigated on cecal ligation and puncture (CLP)-induced polymicrobial sepsis model in rats. In the present study, Sprague-Dawley rats were divided into 5 groups (i.e. the Sham, CLP, JWH-133 0.2 mg/kg, JWH-133 1 mg/kg, and JWH-133 5 mg/kg Groups). Except for the Sham Group, the CLP-induced sepsis model was applied to all groups. The histopathological damage in brain, lung, liver and, heart was examined and the caspase-3, p-NF-kappa B, TNF-alpha, IL-1 beta and IL-6 levels were determined immunohistochemically. The serum TNF-alpha, IL-1 beta, IL-6, IL-10 levels were examined with the ELISA Method. The JWH-133 treatment decreased the histopathological damage in brain, heart, lung, and liver and reduced the caspase-3, p-NF-kappa B, TNF alpha, IL-1 beta, IL-6 levels in these tissues. In addition to this, JWH-133 treatment also decreased the serum TNF-alpha, IL-1 beta, IL-6 levels, which were increased due to CLP, and increased the anti-inflammatory cytokine IL-10 levels. In the present study, it was determined that the CB2 receptor agonist JWH-133 decreases the CLP-induced inflammation, and reduces the damage in brain, lung, liver and heart. Our findings show the therapeutic potential of the activation of CB2 receptors with JWH-133 in sepsis.Öğe Antioxidant Effect of Apelin on Renal Iischemia/Reperfusion Damage(Wiley-Blackwell, 2015) Bircan, Burak; Cakir, Murat; Kirbag, Sevda[Abstract Not Available]Öğe Cannabinoid Type 2 Receptor Activation Reduces Inflammation and Apoptosis in the Lung in Model of Sepsis Induced by Cecal Ligation Puncture(Wiley, 2019) Cakir, Murat; Tekin, Suat; Doganyigit, Zuleyha; Oflamaz, Asli Okan[Abstract Not Available]Öğe Cannabinoid type 2 receptor agonist JWH-133, attenuates Okadaic acid induced spatial memory impairment and neurodegeneration in rats(Pergamon-Elsevier Science Ltd, 2019) Cakir, Murat; Tekin, Suat; Doganyigit, Zuleyha; Erden, Yavuz; Soyturk, Merve; Cigremis, Yilmaz; Sandal, SuleymanAim: Cannabinoid system has various physiological roles such as neurogenesis, synaptic plasticity and emotional state regulation in the body. The presence of cannabinoid type 2 receptor (CB2), a member of the cannabinoid system, was detected in different regions of the brain. CB2 receptor plays a role in neuroinflammatory and neurodegenerative processes. We aimed to determine the possible effect of CB2 agonist JWH-133 in Okadaic acid (OKA)-induced neurodegeneration model mimicking Alzheimer's Disease (AD) through tau pathology. Materials and methods: In this study, 40 Sprague Dawley male rats were divided into 4 groups (Control, Sham, OKA, OKA + JWH-133). Bilateral intracerebroventricular (icv) injection of 200 ng OKA was performed in the OKA group. In the OKA + JWH-133 group, injection of JWH-133 (0.2 mg/kg) was performed intraperitoneally for 13 days different from the group of OKA. Morris water maze test was used to evaluate the spatial memory. Levels of caspase-3, phosphorylated tau (ser396), amyloid beta (A beta), tumor necrosis factor-alpha (TNF-alpha) and interleukin-1 beta (IL-1 beta) levels in brain cortex; and the hippocampus regions were examined by immunohistochemical methods. Key findings: In the OKA group, caspase-3, phosphorylated tau (ser396), A beta, IL-1 beta levels were higher in the cortex and hippocampus than in the other groups. The implementation of the JWH-133 reversed the increments in these parameters, and also prevented spatial memory impairment.Öğe Cannabinoid Type 2 Receptors Activation Improves Cognitive Dysfunction in a Okadaic Acid Induced Alzheimer Rat Model(Karger, 2018) Cakir, Murat; Tekin, Suat[Abstract Not Available]Öğe Cannabinoid type 2 receptors activation ımproves cognitive dysfunction in a okadaic acid ınducedalzheimer rat model(Karger, allschwılerstrasse 10, ch-4009 basel, swıtzerland, 2018) Cakir, Murat; Tekin, SuatÖğe Decreased levels of plasma preptin in female patients with knee osteoarthritis(2019) Cakir, Murat; Sabah Ozcan, Seda; Korkmaz, Murat; Durusoy, SerhatAim: Osteoarthritis (OA) is a common joint disease which is caused by the effects of mechanical, genetic and biochemical factors. In studies conducted in recent years, it has been shown that osteoblasts play roles in OA pathogenesis. In this study, we investigated the relation between the level of preptin, which is known to have an effect on osteoblast proliferation and differentiation, bone metabolism, and the OA disease.Materials and Methods: A total of 40 healthy control patients and 40 women who were diagnosed with knee OA were included in the present study. The plasma preptin levels of the individuals who were included in the study were measured with Enzyme-linked Immuno-Sorbent Assay (ELISA) Method. Blood glucose, triglyceride, High-Density Lipoprotein Cholesterol (HDL-c), Low-Density Lipoprotein Cholesterol (LDL-c), total cholesterol levels and Body Mass Indices (BMI) leukocyte, lymphocyte, neutrophil, Erythrocyte Sedimentation Rate (ESR), C-Reactive Protein (CRP) of all participants were measured.Results: The preptin levels of the knee OA patients in plasma were significantly lower (206 ± 103 ng/L) compared to the healthy control Group (501 ± 525 ng/L) (p0.001). In addition, the (CRP) levels (p0.02) and the Body Mass Indices (BMI) (p0.001) were also higher in the OA Group compared to the Control Group. Conclusion: In the present study, it was determined that there is a significant relation between knee OA disease and plasma preptin levels. Preptin may have roles in the pathogenesis of the OA disease. Further studies are needed to elucidate the mechanisms through which molecular mechanisms preptin is related to knee OA. Keywords: Osteoarthritis; knee; preptin; ELISA; plasma.Öğe Decreased serum periostin level is associated with carpal tunnel syndrome(2020) Sabah Ozcan, Seda; Sacmaci, Hikmet; Cakir, MuratAim: Noninflammatory subsynovial connective tissue (SSCT) fibrosis with nerve compression is an obvious feature of carpal tunnel syndrome (CTS). We investigated serum levels of periostin, which is known to have an effect on fibrosis, in patients with CTS. Material and Methods: A total of 39 healthy individuals and 32 patients who were newly diagnosed with CTS were included in this prospective clinical study. Duration of symptoms, severity, unilateral or bilateral side of CTS diagnosis of patients were recorded. Serum samples were received for the measurement of periostin levels from all participants and were analyzed using commercial enzyme-linked immunosorbent assay (ELISA) kits. Results: Serum periostin levels were significantly lower in CTS patients than controls (p=0.030). Additionally, there was no correlation between serum periostin levels and CTS disease regarding the duration of symptoms and electrophysiological severity of symptoms (p > 0.05).Conclusion: In the current study, it was evaluated that there is a significant relation between CTS and serum periostin levels. Periostin may have roles in the pathogenesis of the CTS. This is the first study to determine serum periostin levels in CTS patients.Öğe Effect of ACA on Ischemia/Reperfusion Injury in Rats (A Histological Study)(Wiley, 2018) Cakir, Murat; Taslidere, Asli; Tekin, Suat; Duzova, Halil[Abstract Not Available]Öğe Effect of apelin hormone on renal ischemia/reperfusion induced oxidative damage in rats(Taylor & Francis Ltd, 2016) Bircan, Burak; Cakir, Murat; Kirbag, Sevda; Gul, Huseyin FatihApelin is a peptide hormone defined as a ligand for G-protein clamped receptor (APJ) receptor. It is indicated in the literature both apelin and APJ are synthesized on the peripheral tissues including the renal tissues. Which roles does the apelin play on the renal tissue has not been completely illuminated yet. This study is designed to determine the possible protective effect of apelin-13 on the kidney I/R injury. Adult male Sprague-Dawley rats were used in this study. In the sham group, right kidneys of the animals were dissected. In the I/R group, right kidney was dissected and ischemia of 45min was performed, and then reperfusion was applied for 3h. In the treatment groups, three different doses of apelin were injected at the beginning of the ischemia unlike the I/R group. BUN, Cre, Na, K, Cl, total protein and albumin from serum samples were determined and TNF-, IL-1, IL-6, TAS and TOS parameters were read with ELISA reader. MDA, SOD, CAT and GSH-Px enzyme activations from renal tissues were measured. In comparison with the sham and I/R groups, while the serum BUN, CRE, CI and TNF- levels showed an increase in the groups on which the apelin-13 was applied, Na, total protein, albumin, TAS levels decreased. Serum TOS level of other groups showed an increase by comparison with the sham group. Our results showed that apelin-13 applied after I/R increased the antioxidant enzyme activity in a dose dependent manner, prevented the lipid oxidation and improved the renal functions.Öğe Effect of Apelin-13 on Liver Damage Following the Renal Ischemia/Reperfusion(Wiley-Blackwell, 2015) Bircan, Burak; Cakir, Murat; Kirbag, Sevda[Abstract Not Available]Öğe Effect of Cannabinoid Type 2 Receptor Activation in Okadaic Acid Induced Rat Alzheimer's Disease Model(Wiley, 2018) Cakir, Murat; Doganyigit, Zuleyha; Tekin, Suat; Erden, Yavuz; Cigremis, Yilmaz; Sandal, Suleyman[Abstract Not Available]Öğe The Effect of Capsaicin on Oxidative Stress Parameters in Kidney in Sepsis Model Induced by Cecal Ligation and Puncture(Wiley, 2023) Firat, Semanur; Bircan, Burak; Cakir, Murat; Sekerci, Guldeniz; Oz, Samet[Abstract Not Available]Öğe The effect of dexmedetomidine against oxidative and tubular damage induced by renal ischemia reperfusion in rats(Taylor & Francis Ltd, 2015) Cakir, Murat; Polat, Alaadin; Tekin, Suat; Vardi, Nigar; Taslidere, Elif; Duran, Zeynep Rumeysa; Tanbek, KevserDexmedetomidine (dex) is a potent, highly selective and specific alpha 2-adrenoreceptor agonist. This experimental study was designed to investigate protective and therapeutic effect of two different doses of dex, on kidney damage induced by ischemia-reperfusion (I/R) in rats. Male Sprague - Dawley rats were divided into four groups, each including 10 animals: control group, ischemia-reperfusion (I/R) group; treated groups with 10 mu g/kg of dex and 100 mu g/kg of dex. After removing right kidney of the rats, the left kidney has performed ischemia during 40 min and reperfusion in the following 3 h. The histopathological findings, and also tissue superoxide dismutase (SOD) and catalase (CAT) enzyme activity, malondialdehyde (MDA), glutathione (GSH), serum blood urea nitrogen (BUN), creatinine (Cre) and tumor necrosis factor-alpha (TNF-alpha) levels were determined. In the I/R group, compared to the control group, levels of BUN, Cre and kidney tissue MDA have increased significantly, SOD, CAT enzyme activity and glutathione levels have decreased significantly. In the dex10 group, compared to the I/R group, levels of Cre and TNF-alpha have decreased significantly, while the SOD activity has increased significantly. In the dex100 group, compared to the I/R group, levels of BUN, Cre have decreased significantly, while the SOD activity has increased significantly. In the I/R group, there was also extensive tubular necrosis, glomerular damage in the histological evaluation. Dex ameliorated these histological damages in different amounts in two treatment groups. In this study, the protective effects of dex against renal I/R injury have been evaluated by two different amount of doses.Öğe The effect of hypericum perforatum on kidney ischemia/reperfusion damage(Taylor & Francis Ltd, 2017) Cakir, Murat; Duzova, Halil; Baysal, Isil; Gul, Cemile Ceren; Kuscu, Gulbahar; Kutluk, Fatma; Cakin, HilalIt has been revealed in recent studies that Hypericum Perforatum (HP) is influential on cancer, inflammatory diseases, bacterial and viral diseases, and has neuroprotective and antioxidant properties. In this study, we investigated the effect of HP, which is known to have antioxidant and anti-inflammatory effects, on kidney I/R damage. Male Sprague-Dawley rats were divided into three groups, and each of the groups had eight rats: The Control Group; the Ischemia/Reperfusion (I/R) Group; and the IR+HP Group which was treated with 50mg/kg of HP. The right kidneys of the rats were removed, and the left kidney developed ischemia during the 45th min, and reperfusion occurred in the following 3rd h. The histopathological findings and also the level of Malondialdehyde (MDA), Glutathione (GSH) and superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-PX) enzyme activations in the renal tissues were measured. Blood Urea Nitrogen (BUN), Creatinin (Cre) from serum samples were determined. The levels of BUN, Cre, and kidney tissue MDA increased at a significant level, and the SOD, CAT, and GSH-PX enzyme activity decreased at a significant level in the I/R group, compared with the Control Group (p < 0.05). In the I/R vertical bar HP group, the levels of MDA decreased at a significant level compared to the I/R group, while the SOD, CAT, and GSH-PX activity increased (p < 0.05). In histopathological examinations, it was observed that the tubular dilatation and epithelial desquamation regressed in the IR vertical bar HP Group when compared with the I/R Group. It has been shown with the histological and biochemical results in this study that HP is protective against acute renal I/R.Öğe Effect of KML29 and URB597 on Renal Ischemia Reperfusion Injury in Rats(Wiley, 2023) Cakir, Murat; Aydin, Ali; Tekin, Suat[Abstract Not Available]Öğe Effect of Salusin-? and Salusin-? on Heart Damage Following the Renal Ischemia/Reperfusion(Wiley-Blackwell, 2016) Cakir, Murat; Taslidere, Asli; Duzova, Halil; Orhan, Guler[Abstract Not Available]Öğe Effect of Salusin-? and Salusin-? on Liver Damage Following the Renal Ischemia/Reperfusion(Wiley, 2017) Cakir, Murat; Taslidere, Asli; Duzova, Halil; Orhan, Guler[Abstract Not Available]Öğe The Effect of TRPA1 Channel Agonist ASP7663 and Antagonist HC-030031 on Oxidative Stress Parameters in Renal Ischemia Reperfusion in Rats(Wiley, 2023) Firat, Semanur; Sekerci, Guldeniz; Cakir, Murat; Bircan, Burak; Oz, Samet[Abstract Not Available]
