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Öğe Enterotoxemia Caused by Clostridium perfringens Type E in a Calf(Kafkas Univ, Veteriner Fakultesi Dergisi, 2018) Eroksuz, Yesari; Otlu, Bads; Calicioglu, Mehmet; Eroksuz, Hatice; Incili, Canan Akdeniz; Karabulut, Burak; Abayli, HasanClostridial enterotoxemia caused by Clostridium perfringens type E was diagnosed postmortem in a 2-months-old calf in a family farm containing 20 cattle at different ages. Varying degrees of severity of segmental fibrino-hemorrhagic and necrotic enteritis was present at the necropsy. Clostridium perfringens was isolated from the intestinal tissue and intestinal content and the genes encoding alpha and iota toxins were detected by polymerase chain reaction (PCR). Histopathological examination showed the presence of adherent Gram-positive rods on the surface of villi and in poly morphonuclear leucocytes in the lamina propria of the intestinal mucosa.Overall, the results of the present study suggest that C. perfringens type E should be considered at differential diagnosis in fibrino-hemorrhagic enteritis and sudden deaths in post weaned calves.Öğe Impact of chitosan embedded with postbiotics from Pediococcus acidilactici against emerging foodborne pathogens in vacuum-packaged frankfurters during refrigerated storage(Elsevier Sci Ltd, 2022) Incili, Gokhan Kursad; Karatepe, Pinar; Akgol, Muzeyyen; Tekin, Ali; Kanmaz, Hilal; Kaya, Busra; Calicioglu, MehmetThe objective of the study was to carry out characterization of postbiotics from Pediococcus acidilactici and to assess their efficacy (50% and 100%) in combination with chitosan (0.5 and 1%) against Escherichia coli O157: H7, Salmonella Typhimurium, Listeria monocytogenes on frankfurters during refrigerated storage for 35 days. High amounts of total phenolic content (1708.15 +/- 93.28 mg GAE/L) and carboxylic acids, which comprised 74.89% of the total volatiles, were found in the postbiotics. On day 0, the postbiotic-chitosan combinations decreased the E. coli O157:H7, L. monocytogenes and S. Typhimurium counts ranging from 1.58 to 3.21 log(10) compared to the control in frankfurters (P < 0.05). Total viable count and number of lactic acid bacteria were effectively reduced in all treatment groups (P < 0.05), and postbiotic and chitosan treatments did not cause any changes in pH and color of the frankfurters. In conclusion, postbiotic-chitosan combinations can be used to reduce the risks that might be associated with E. coli O157:H7, L. monocytogenes, and S. Typhimurium in frankfurters.