Yazar "Coskun, E. I." seçeneğine göre listele

Listeleniyor 1 - 4 / 4
  • Küçük Resim Yok
    Öğe
    Analysis of E6/E7 mRNA gene expression by means of NucliSENS EasyQ (NASBA) technique in patients suffering from cervical dysplasia with HPV positive
    (7847050 Canada Inc, 2017) Ciplak, B.; Yilmaz, E.; Otlu, B.; Coskun, E. I.; Sahin, N.; Tuncay, G.
    Objective: In this study, the authors aimed at examining E6/E7 mRNA gene expression by means of NucliSENS EasyQ (NASBA) in patients in which HPV infection was detected and diagnosed with cervical dysplasia. Materials and Methods: This study included 77 patients diagnosed with cervical dysplasia. The patients were grouped based on cervico-vaginal smear anomalies. Digene HC2 DNA Collection Device transport medium was used for taking and keeping cervical samples, QIA symphony SP device, and QIAsymphony DSP AXpH DNA kit were used for HPV DNA extraction from cervical samples kept in transport medium, HPV Q24 complete kit, RotorGene and PyroMark Q24 for detection of HPV-DNA and determination of HPV types and NucliSENS Easy Q Genetic Analyzer technique for determination of E6/E7 gene expression in HPV positive samples. SPSS (Statistical Package for Social Sciences) Version 22.0 was employed in statistical evaluation of the research date. Results: The most common HPV was considered as type 16 in this study. When pathology specimens of patients in whom dysplasia was detected in their cervical biopsies were examined in terms of HPV E6/E7 mRNA expression, a statistically significant difference was found between the normal and pathology groups (p < 0.05). Conclusion: Today, the presence of HPV infection in etiology of cervical smear pathologies and dysplasia is undisputedly accepted. The main determinant factor of HPV virulence is the frequency of E6 and E7 gene expressions existing in the DNA structure and responsible for virulence. NucliSENS_Ea_syQ (NASBA) is a technique employed to analyze mRNA gene expression in an accurate, reliable, and rapid way. Parallel to the conclusions of studies in the literature, frequency of E6/E7 gene expression increases in proportion to the increasing dysplasia degree of cervical pathologies in the present study as well.
  • Küçük Resim Yok
    Öğe
    Analysis of e6/e7 mrna gene expression by means of nuclisens easyq (nasba) technique in patientssuffering from cervical dysplasia with hpv positive
    (I r o g canada, ınc, 4900 cote st-luc, apt#212, montreal, quebec h3w 2h3, canada, 2017) Ciplak, B.; Yilmaz, E.; Otlu, B.; Coskun, E. I.; Sahin, N.; Tuncay, G
    Objective: In this study, the authors aimed at examining E6/E7 mRNA gene expression by means of NucliSENS EasyQ (NASBA) in patients in which HPV infection was detected and diagnosed with cervical dysplasia. Materials and Methods: This study included 77 patients diagnosed with cervical dysplasia. The patients were grouped based on cervico-vaginal smear anomalies. Digene HC2 DNA Collection Device transport medium was used for taking and keeping cervical samples, QIA symphony SP device, and QIAsymphony DSP AXpH DNA kit were used for HPV DNA extraction from cervical samples kept in transport medium, HPV Q24 complete kit, RotorGene and PyroMark Q24 for detection of HPV-DNA and determination of HPV types and NucliSENS Easy Q Genetic Analyzer technique for determination of E6/E7 gene expression in HPV positive samples. SPSS (Statistical Package for Social Sciences) Version 22.0 was employed in statistical evaluation of the research date. Results: The most common HPV was considered as type 16 in this study. When pathology specimens of patients in whom dysplasia was detected in their cervical biopsies were examined in terms of HPV E6/E7 mRNA expression, a statistically significant difference was found between the normal and pathology groups (p < 0.05). Conclusion: Today, the presence of HPV infection in etiology of cervical smear pathologies and dysplasia is undisputedly accepted. The main determinant factor of HPV virulence is the frequency of E6 and E7 gene expressions existing in the DNA structure and responsible for virulence. NucliSENS_Ea_syQ (NASBA) is a technique employed to analyze mRNA gene expression in an accurate, reliable, and rapid way. Parallel to the conclusions of studies in the literature, frequency of E6/E7 gene expression increases in proportion to the increasing dysplasia degree of cervical pathologies in the present study as well.
  • Küçük Resim Yok
    Öğe
    MPV, NLR, and platelet count: new hematologic markers in diagnosis of malignant ovarian tumor
    (I R O G Canada, Inc, 2017) Yilmaz, E.; Coskun, E. I.; Sahin, N.; Ciplak, B.; Ekici, K.
    Objective: Evaluation of mean platelet volume (MPV), neutrophil to lymphocyte ratio (NLR), and platelet count in the detection of malignant and benign ovarian tumors. Material and Methods: Sixty-six patients admitted to the present clinic over three years were included in the study. Patients diagnosed with ovarian cancer were grouped into the areas of epithelial and granulosa cell tumor diagnosis. The values were compared to patients with benign cystic structure. Patients' preoperative hematologic parameters and their values four weeks postoperatively were analyzed. Statistical analyses were performed with SPSS software. Results: MPV, NLR, and platelet count were observed at a higher rate as statistically significant in patients diagnosed with malignant ovarian cancer, compared to those with benign adnexal mass. Conclusion: The hematological parameters such as MPV, NLR, and platelet count in the detection of malignant ovarian tumors have been evaluated as useful new markers.
  • Küçük Resim Yok
    Öğe
    Nuclear factor-kappa beta pathway and endometrial cancer: a pilot study
    (I R O G Canada, Inc, 2017) Yilmaz, E.; Coskun, E. I.; Gul, M.; Sahin, N.; Tuncay, G.; Simsek, Y.
    Objective: Examination of the role of nuclear factor-kappa beta (NF-kappa B) expression in the etiopathogenesis of endometrial cancer, by means of the immunohistochemical method. Materials and Methods: Patients who applied to participate in the study at the clinic were grouped into three categories: those diagnosed with benign endometrial pathology, those with endometrial hyperplasia, and those with endometrial cancer. NF-kappa B analysis was conducted in the endometrial tissues of the patients' paraffin blocks by means of the immunohistochemical method. For objective assessment purposes, the H score of each patient was calculated. SP SS 15.0 program was employed for statistical analysis. Results: The average H score of the first group, comprising benign endometrial pathologies, was 102.4 +/- 85.9, that of the hyperplasia group was 143.6 +/- 122.4, and that of the cancer group was 276.8 +/- 61.8. The average values of groups 1 and 2 were similar (p = 0.349); however, the third group's average H score was significantly higher (p < 0.001). Conclusion: NF-kappa B, which is a critical mediator in the inflammation process, might be related to the development of premalign and malign endometrial changes.