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Öğe Effect of thymoquinone on transient receptor potential melastatin (TRPM) channels in rats with liver ischemia reperfusion model in rats(Mashhad Univ Med Sciences, 2024) Caglar, Kerem; Dokuyucu, Recep; Agturk, Gokhan; Tumer, Cemil; Tutuk, Okan; Gocmen, Hatice Dogan; Gokce, HasanObjective(s): We aimed to investigate the levels of transient receptor potential melastatin (TRPM) gene expression, and the antioxidant and histopathologic effect of thymoquinone (Tmq) in the hepatic I/R rat model. Materials and Methods: Fifty Wistar rats were divided into 5 groups. Group 1: Control; Group 2: Sham; Group 3: Hepatic I/R (45 min/45 min); Group 4: Tmq (50 mg/kg); Group 5: Tmq+I/R (ten days before I/R at the dose of 50 mg/kg of Tmq). The hepatic I/R (45min/45min) model was performed at the portal vein and the hepatic artery with atraumatic vascular clamp in the ischemia groups. The liver tissues and blood samples that were taken at the end of the study were evaluated for histopathologic and biochemical analysis. Besides TRPM gene expression levels were determined in liver tissues. It was seen that cellular swelling, congestion, PNL, and apoptosis parameters statistically decreased in Tmq and Tmq+I/R groups in comparison with the I/R group in histopathological evaluation. Results: It was observed that biochemical parameters, AST, ALT, GGT, LDH, creatinine, and urea levels significantly increased in the I/R group as compared with, sham, Tmq, and Tmq+I/R groups. It was found that TRPM2,6,7,8 gene expression decreased significantly in Tmq+I/R groups as compared to the I/R group. Conclusion: We showed that thymoquinone can inhibit the entry of Ca+2 into the cell by decreasing TRPM2,6,7,8 gene expression. Based on our findings, we think that Tmq application in the treatment of liver diseases due to I/R damage may be important in terms of both ischemia and apoptosis and can also be used in the treatment of liver-related diseases.Öğe Effects of Alpha-Lipoic Acid on TGF-?1 and Urotensin-II Levels in Glucocorticoid-Induced Osteonecrosis in Rats(Kowsar Publ, 2020) Koca, Irfan; Dokuyucu, Recep; Tas, Zeynel Abidin; Gokce, Hasan; Ozcan, OguzhanBackground: Osteonecrosis (ON) is a serious health problem, which dramatically reduces the quality of life. Objectives: In the present study on the rat model of glucocorticoids (GCs) -induced ON, we explored the influence of alpha-lipoic acid on serum levels of TGF-fi1 and urotensin-II (U-II) and on histological alteration with respect to fatty degeneration and osteocyte necrosis. Methods: A total of 32 male Wistar albino rats were equally assigned to four groups, including control, methylprednisolone acetate (MPA), alpha-lipoic acid (ALA), and MPA with ALA (MP1 + ALA). The animals in MPA group subcutaneously received 15 mg/kg/week during 2 weeks, whereas 100 mg/kg/day ALA was intraperitoneally administered to ALA group during 4 weeks. The MPA + ALA group had both treatments with the same doses. ON was confirmed and graded histologically. Lipid peroxidation and DNA damage levels were immunohistochemically assessed in rats' bones. Results: After histopathological examinations, ALA injection attenuated oxidative stress levels through reducing both 8-OHdG-and 4-HNE-positive cells in the femoral head region (P < 0.05). The U-II and TGF-fi1 protein levels significantly decreased after ALA treatment in MPA injected animals (P < 0.05, P < 0.01, respectively). Moreover, there was a strong correlation between U-II and TGF-fi 1 protein levels (P = 0.019, r = 0.884). Conclusions: This study is novel with regard to showing the therapeutic effects of ALA on GC-inducedONin rats as well as the strong correlation between the expression levels of U-II and TGF-fi1 proteins. In this regard, ALAmaybe a therapeutic agent in the treatment of ON patients.Öğe Investigation of the Effect of Verapamil on Hepatic Ischemia Reperfusion Injury(Wiley-Blackwell, 2016) Dokuyucu, Recep; Dogan, Hatice; Izmirli, Muzeyyen; Bilgic, Nilufer; Gokce, Hasan[Abstract Not Available]Öğe The Role of Lidocaine on Transient Receptor Potential Melastatin (TRPM) 6,7 Channels in Hepatic Ischemia/Reperfusion Rat Model(Wiley-Blackwell, 2016) Dokuyucu, Recep; Tutuk, Okan; Dogan, Hatice; Izmirli, Muzeyyen; Bilgic, Nilufer; Gokce, Hasan[Abstract Not Available]
