Yazar "Erdogan, Mehmet Mustafa" seçeneğine göre listele

Listeleniyor 1 - 3 / 3
  • Küçük Resim Yok
    Öğe
    Effect of Melatonin on Increasing the Effectiveness of Liver Preservation Solution
    (Aves, 2023) Erdogan, Mehmet Mustafa; Erdemli, Mehmet Erman; Ozhan, Onural; Erdemli, Zeynep; Bag, Harika Gozde Gozukara; Vardi, Nigar
    Background/Aims: Various tissue preservation solutions are used during the removal of the organ and during transplantation to protect the normal histological and biochemical characteristics of tissue while performing a successful liver transplant. In our study, it was aimed to investigate the effects of intraperitoneal melatonin administration on liver preservation damage before transplantation. Materials and Methods: In our study, the histological and biochemical characteristics of University of Wisconsin + melatonin group rats treated with melatonin 45 minutes before hepatectomy were compared between serum physiologic group and University of Wisconsin group. Results: When hematoxylin and eosin staining was evaluated in terms of hydropic degeneration, sinusoidal dilatation, and hepatocyte necrosis, there was no statistically significant difference. Caspase 3 immunohistochemical staining showed a significant increase in Caspase 3 immunoreactivity positivity at the 12th-hour University of Wisconsin group compared to University of Wisconsin + melatonin group. As a result of biochemical analysis, the malondialdehyde and total oxidant status levels in the University of Wisconsin + melatonin group decreased significantly compared to the University of Wisconsin group. When the reduced glutathione activity and total antioxidant capacity level were compared, a significant increase was observed in the University of Wisconsin + melatonin group compared to the University of Wisconsin group at the 12(th) hour. It was also found that aspartate aminotransferase, alanine aminotransferase, and alkaline phosphatase levels decreased significantly in the University of Wisconsin + melatonin 12(th)-hour group compared to the University of Wisconsin 12(th) hour and control group. Conclusion: When the findings were evaluated, intraperitoneal administration of melatonin, a cytoprotective antioxidant, was found to play an effective role in preserving immunohistochemical and biochemical properties of liver tissue integrity and hepatocytes in University of Wisconsin solution.
  • Küçük Resim Yok
    Öğe
    Hepatoprotective effects of N-acetylcysteine on liver injury by irisin upregulation and oxidative stress reduction in diabetic rats
    (Springeropen, 2023) Erdogan, Mehmet Mustafa; Erdogan, Mehmet Ali; Koc, Suleyman; Yalcin, Alper; Turk, Ahmet; Yetkin, Esra Akkus
    BackgroundThe current study aimed to investigate the oxidative stress in rat liver with diabetes mellitus (DM) as well as the protective effects of N-acetylcysteine (NAC) on irisin expression.MethodsTwenty-eight male Wistar rats were divided into four groups, 7 rats in each group, and 30-day regimens of experimental or control groups. NAC-treated group is as follows: 100 mg/kg once daily was administered intraperitoneally (i.p.). Diabetes-induced group is as follows: single-dose intraperitoneal injection of streptozotocin (STZ) (50 mg/kg) was used to induce DM in overnight fasting Wistar rats. By determining blood glucose concentration in STZ-induced rats 72 h after injection of STZ, DM was assessed. DM + NAC group is as follows: STZ-induced DM plus NAC is described previously. On the 30th day of the experiment, liver samples were collected after fasting and anesthesia. Biochemical analyses were performed to measure total antioxidant status (TAS), total oxidant status (TOS), and malondialdehyde (MDA) levels. Each liver sample was weighed and then prepared for histopathologic evaluation by light microscopy.ResultsThere was a statistically significant decrease in TAS levels and an increase in TOS and MDA levels in the DM group compared to the control group. In contrast, TOS and MDA levels were found significantly decreased, and TAS levels increased in the serum and liver tissues of the DM + NAC group compared to the DM group. Liver samples were also used for histopathological examination using hematoxylin-eosin and immunohistochemical staining. STZ-induced liver damage was detected as oxidative stress, increased irisin immunoreactivity, sinusoidal dilatation, and hepatocyte degeneration. In the DM + NAC group, it was observed that NAC significantly reduced the aforementioned histopathological changes due to STZ.ConclusionIn the early period of diabetes, due to the antioxidant properties of irisin related to the sudden response of liver tissue to oxidative stress, it is thought that the immunoreactivity in the tissue increases in the early period. As a result, NAC in diabetic rat liver tissue was found to suppress oxidative damage and irisin immunoreactivity.
  • Küçük Resim Yok
    Öğe
    The diagnostic potential of Asprosin and Meteorin-like proteins in basal and squamous cell carcinomas
    (Springer, 2025) Erdogan, Mehmet Mustafa; Erdogan, Esra; Kocaman, Nevin; Yasar, Seyma; Kavak, Songul Yerlikaya
    Non-melanoma skin cancer (NMSC) is the most prevalent malignancy worldwide, encompassing primarily BCC and SCC. Differentiating between these two carcinomas and distinguishing them from normal skin tissue can pose diagnostic challenges in certain cases. Adipokines, as regulators of various biological processes, have garnered attention for their potential roles in tumorigenesis. Asprosin (Asp) and Meteorin-like (Metrnl) are newly identified proteins implicated in several diseases, including cancer. Their potential contribution to the pathogenesis of skin cancers such as BCC and SCC remains underexplored. This study aimed to evaluate the expression profiles of Asp and Metrnl in BCC and SCC and to assess their potential utility as diagnostic biomarkers. The expression of Asp and Metrnl proteins was examined by immunohistochemical staining in biopsy samples from 60 control, 60 BCC, and 60 SCC cases obtained from the archive of the Pathology Laboratory of Malatya Turgut Ozal University Training and Research Hospital. Asp immunoreactivity was higher in BCC tissues compared to the other two groups, and it was statistically higher in SCC tissues compared to healthy control tissues. Metrnl immunoreactivity was also higher in BCC tissues compared to the other two groups, and statistically significant in SCC tissues when compared to healthy control tissues. The findings suggest that Asp and Metrnl proteins may serve as valuable biomarkers for distinguishing between BCC and SCC, the two most common non-melanoma skin cancers encountered in routine dermatopathology practice. These adipokines may also provide insights into the underlying pathophysiology of these malignancies, offering potential avenues for diagnostic and therapeutic advancements.