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    The investigation of polymorphisms in DNA repair genes (XRCC1, APE1 and XPD) in women with polycystic ovary syndrome
    (Asian Pacific Organization for Cancer Prevention, 2017) Gulbay G.; Yesilada E.; Celik O.; Yologlu S.
    Background: PCOS was reported to arise from the interaction of genetic and environmental factors. Some studies reported that women with PCOS have DNA damage and chromosome breakage. Such studies bring to mind the genes that are involved in DNA repairing. At present, several DNA repair genes and, as products of these genes, certain polymorphisms that alter the activity of proteins are known in the literature. The aim of this dissertation is to study the genomic instability that have been reported in PCOS cases along with the relationship between XRCC1 Arg194Trp, XRCC1 Arg399Gln, APE1 Asp148Glu, and XPD Lys751Gln polymorphisms in order to contribute to the pathogenesis of PCOS. Methods: Polymorphisms in DNA repair genes have been associated with the increased risk of various diseases and could also be related to the etiology of PCOS. Therefore, we conducted a study including 114 women with PCOS and 91 controls. These polymorphisms were determined by quantitative real time PCR and melting curve analysis using LightCycler. Results: Comparing the control groups at the end of the study, the results have not shown any statistically significant difference as far as XRCC1 Arg194Trp, XRCC1 Arg399Gln, and XPD Lys751Gln polymorphisms are concerned. However, there were notable differences between the groups in terms of APE1 Asp148Glu polymorphism. Associated with this condition, it has been noted that both mutant allele (Glu) frequency (37.72 % in the study group; 19.23% in the control group, p=0.0001) and homozygous mutant genotype (Glu/Glu) frequency (%12.28 in the study group; %6.60 in the control group, p=0.015) have been higher in the study group.
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    Protective effect of myricetin against e2-induced genotoxic damage in human lymphocytes
    (2012) Yuksel S.; Yesilada E.; Gulbay G.; Kurtoglu E.; Serap Savaci S.
    Protective effect of myricetin (MRY) against the cytotoxic and genotoxic effects of a hormonal steroid, 17 ßestradiol (E2), was assessed in peripheral blood human lymphocyte culture. Sister chromatid exchanges (SCE), mitotic index (MI) and replication index (RI) were scored as genetic endpoints. Firstly, the genotoxic effect of different amounts (5, 10 and 20 ?M final concentration) of E2 was tested, and 10 and 20 ?M E2 levels were detected as genotoxic. In the second set, E2 groups were treated with 10 ?M MRY. MRY reduced the SCE but increased MI as well as RI, suggesting its protective action on human lymphocytes in vitro against E2-induced genotoxic damage. © by PSP.