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    Biostimulatory Effects of Diode Laser and Ozone on Wound Healing in Rats
    (Galenos Yayincilik, 2018) Alan, Hilal; Guler, Cigdem; Yolcu, Umit; Koparal, Mahmut; Cakir, Ebru; Demir, Pinar
    Objective: Diode laser and ozone can be used to healing of skin wounds. The aim of this study is to evaluate the biostimulatory effects of diode laser and ozone on the healing of sutured skin wounds in rats. Materials and Methods: In this study, 21 adult males Wistar albino rats were used. Three 1-cm-longs, full-thickness incisions were applied to the back of each animal and closed with sutures. Diode laser treatment, irrigation with 0.9% saline solution and ozone therapy was applied to the wounds through 7 days. Animals were euthanized on the 3rd, 7th, and 14th days after treatment, and all wounds were surgically removed for histopathological evaluation. Results: According to histological results, a statistically significant difference was found among the three treatment methods in acute inflammatory reactions on 3rd day (p<0.05). Ozone treatment reduced the intensity of acute inflammatory reactions by the 3rd day (p<0.05). In addition, a statistically significant difference was found between the follow-up periods in all treatment groups for fibrosis (p<0.01). Conclusion: It was found that ozone treatment was more effective when compared to the other treatments for reducing the intensity of acute inflammatory reactions on the healing of sutured skin wounds.
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    Effects of Er:YAG Laser on Mineral Content of Sound Dentin in Primary Teeth
    (Hindawi Ltd, 2014) Guler, Cigdem; Malkoc, Meral Arslan; Gorgen, Veli Alper; Dilber, Erhan; Bulbul, Mehmet
    The aim of the present study was to evaluate the mineral content of sound dentin in primary teeth prepared using an Er:YAG laser at two different power settings. Thirty-six primary second molars were used in this study. Three dentin slabs were obtained from each tooth, and the slabs were randomly divided into three groups: Group A, control; Group B, Er: YAG laser at 3.5 W, 175 mJ, and 20 Hz, short pulse mode; and Group C, Er: YAG laser at 4 W, 200 mJ, and 20 Hz, medium-short pulse mode. One dentin slab per group was used to evaluate the dentinal morphology and surface roughness values using SEM and profilometer, respectively. Mineral content in the dentin slabs were calculated by inductively coupled plasma-atomic emission spectrometry (ICP-AES). The data were analyzed by one-way analysis of variance and Tukey's HSD tests. No significant differences in Ca, K, Mg, Na, and P levels or Ca/P ratio were found among the groups (P > 0.05). SEM micrographs showed that surface irregularities increased with a higher power setting. The surface roughness after laser treatment in Group B and Group C was found to be similar, unlike Group A.
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    Evaluation of salivary total oxidant-antioxidant status and DNA damage of children undergoing fixed orthodontic therapy
    (E H Angle Education Research Foundation, Inc, 2015) Guler, Cigdem; Toy, Ebubekir; Ozturk, Firat; Gunes, Dilek; Karabulut, Aysun Bay; Otlu, Onder
    Objective: To determine the total oxidant status (TOS), total antioxidant status (TAS), and the 8-hydroxy-2'-deoxyguanosine (8-OHdG) levels and their interrelationship in the saliva of children undergoing fixed orthodontic therapy. Materials and Methods: Thirty children were randomly divided into three groups. The attachments were bonded to all of the teeth using three different orthodontic composites: Transbond XT, Kurasper F, and GrenGloo. The salivary levels of TOS, TAS, and 8-OHdG were determined three times, as follows: before treatment (T-1) and at 1 month (T-2) and 3 months (T-3) following appliance placement. All data were statistically analyzed. Results: There were no significant differences in TOS, TAS, and 8-OHdG within the same time periods among the three different orthodontic composites (P > .05). TAS in all composite groups decreased over time. These decreases were found to be significant for Kurasper F and GrenGloo at the T1 T3 and T2 T3 time periods (P < .05). In all composite groups 8-OHdG decreased between T1 and T2 (P < .05). However, 8-OHdG in all composite groups increased from T2 to T3. These differences in 8-OHdG were significant in Kurasper F and GrenGloo (P < .05). Conclusions: Fixed orthodontic appliances bonded with the tested composites did not increase the cytotoxicity markers in saliva.
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    Evaluation of tooth-fragment reattachment: a clinical and laboratory study
    (Wiley-Blackwell, 2010) Yilmaz, Yucel; Guler, Cigdem; Sahin, Hakan; Eyuboglu, Ozge
    Purpose: To evaluate the restoration of fractured teeth by reattaching tooth fragment to its tooth remnant in a group of children and adolescents, and to compare the results with those of a laboratory study. Materials and Methods: The clinical study was conducted on 43 fractured incisors: 22 uncomplicated crown fractures (Group A) and 21 complicated crown fractures (Group B). The 43 incisal fragments: 23 were kept dry for 47 h and 20 were kept wet for 24 h by the patients before they were reattached. The fragments were kept in 0.9% saline solution for 30 min before reattachment. The fragments in Group A were reattached using a dentin bonding agent, a flowable and a hybrid resin composite, whereas the fragments in Group B were reattached to the tooth remnant after a pulpotomy was performed. The laboratory study was conducted on 56 extracted incisors. Teeth were divided equally into four groups: Group I - Uncomplicated crown fracture + wet medium; Group II - Uncomplicated crown fracture + dry medium; Group III - Complicated crown fracture + wet medium, and Group IV - Complicated crown fracture + dry medium. The fragments were then reattached in a manner that was similar to that used in the clinical study. The restored teeth were then re-fractured. All data were analyzed statistically. Results: In the clinical study, the restored teeth were followed up for 2 years. Neither the type of trauma nor the storage medium had any significant effect on the survival, color, and bond strength of the restored teeth when assessed in the clinical and laboratory study. The color disharmony that was encountered initially in restored teeth resolved significantly on its own accord within 12 months after reattachment of the fragment. Conclusion: Fragment reattachment can be used to treat fractured teeth successfully in children and adolescents.
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    Is More Cortical Bone Decortication Effective on Guided Bone Augmentation?
    (Lippincott Williams & Wilkins, 2016) Acar, Ahmet Hueseyin; Alan, Hilal; Ozgur, Cem; Vardi, Nigar; Asutay, Fatih; Guler, Cigdem
    This study aims to evaluate the possible effect of more cortical bone decortication (CBD) on guided bone augmentation. A total of 16 New Zealand rabbits and 32 titanium domes were used. No cortical bone decortication was applied to the control group and in the study groups, the cortical bones were decorticated with a round burr (Group A: 1 hole with bleeding, Group B: 5 holes with bleeding, Group C: a thin layer of compact bone was completely removed with no bleeding). Then 2 titanium domes were placed on the calvarium of each rabbit with hydroxyapatite/beta-tricalcium phosphate. After 3 months, the animals were sacrificed and specimens were sent for histological and histomorphometric analysis. Histological and histomorphometric analysis showed that bone decortication with burr significantly increased new bone regeneration in all the experimental groups compared with the control group (P<0.05). No statistically significant difference was determined between the study groups. In conclusion, CBD, which has no negative impact on surgery, has a positive effect on guided bone augmentation. However, a greater amount of CBD does not have a greater effect.