Yazar "Gursoy, Nafia Canan" seçeneğine göre listele

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    Brain abscess and bronchopneumonia caused by Acinetobacter baumannii in a 2-year-old female sheep
    (Taylor & Francis Ltd, 2018) Eroksuz, Yesari; Otlu, Baris; Eroksuz, Hatice; Gursoy, Nafia Canan; Yerlikaya, Zeynep; Incili, Canan Akdeniz; Karabulut, Burak
    [Abstract Not Available]
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    Brain abscess and bronchopneumonia caused by acinetobacter baumannii in a 2-year-old femalesheep
    (Taylor & francıs ltd, 2-4 park square, mılton park, abıngdon or14 4rn, oxon, england, 2018) Eroksuz, Yesari; Otlu, Baris; Eroksuz, Hatice; Gursoy, Nafia Canan; Yerlikaya, Zeynep; Incili, Canan Akdeniz; Karabulut, Burak; Timurkaan, Necati; Timurkan, Mehmet Ozkan
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    A Case of Tubo-ovarian Abscess due to Salmonella enterica Following an In Vitro Fertilization Attempt
    (Galenos Yayincilik, 2019) Yakupogullari, Yusuf; Isik, Burak; Gursoy, Nafia Canan; Bayindir, Yasar; Otlu, Baris
    [Abstract Not Available]
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    Clinical Strains of Chryseobacterium and Elizabethkingia spp. Isolated from Pediatric Patients in a University Hospital: Performance of MALDI-TOF MS-Based Identification, Antimicrobial Susceptibilities, and Baseline Patient Characteristics
    (Mary Ann Liebert, Inc, 2018) Mirza, Hasan Cenk; Tuncer, Ozlem; Olmez, Serpil; Sener, Burcin; Tugcu, Gokcen Dilsa; Ozcelik, Ugur; Gursoy, Nafia Canan
    Our objective was to evaluate the performance of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) for identification of the Chryseobacterium and Elizabethkingia spp. isolated from pediatric patients at Hacettepe University Hospital using 16S rRNA gene sequencing as the gold standard and to determine the antimicrobial susceptibility patterns of the isolates and baseline characteristics of patients. All stored Chryseobacterium and Elizabethkingia spp. isolated from various clinical specimens (sputum, blood, and urine) of pediatric patients at Hacettepe University Hospital between 2012 and 2016 were included in this study. Minimum inhibitory concentrations of 10 antimicrobial agents were determined by Etest for all isolates. To determine the baseline characteristics of patients, medical records of all patients were retrospectively reviewed. In total, 18 isolates of Chryseobacterium spp. (16 C. indologenes, 2 C. gleum) and 5 isolates of Elizabethkingia spp. (3 E. meningoseptica, 2 E. anophelis) were identified by 16S rRNA sequencing. MALDI-TOF MS correctly identified 19 (82.6%) isolates to the species level. The quinolones (ciprofloxacin and levofloxacin), trimethoprim/sulfamethoxazole and piperacillin/tazobactam showed the highest spectrum of activity against the overall collection of isolates. Cystic fibrosis (CF) was the underlying disease in 81.8% of patients. To our knowledge, this study includes the largest number of Chryseobacterium spp. isolated from clinical specimens of pediatric patients in Turkey. In this study, we also report the first clinical isolate of E. anophelis in Turkey. Since, the majority of strains were isolated from patients with CF; larger, prospective clinical studies are needed to establish whether chryseobacteria could be considered as an emerging opportunistic pathogen in patients with CF.
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    Comparison of Conventional Methods, Automated Systems, and DNA Sequence Analysis Methods in the Identification of Corynebacterium afermentans and Corynebacterium mucifaciens Bacteria Isolated from Blood and Catheter Culture Samples
    (Mary Ann Liebert, Inc, 2021) Olmez, Serpil; Tuncer, Ozlem; Parlak, Mehmet; Bicakcigil, Asiye; Gursoy, Nafia Canan; Otlu, Baris; Guducuoglu, Huseyin
    The aim of this study is to compare different methods due to the difficulties in identifying coryneform bacteria to species level and to determine antibiotic resistance profiles. Isolates identified as Turicella otitidis (n:45) by VITEK 2 Compact and Corynebacterium mucifaciens (n:1) by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS), isolated from blood and catheter cultures between 2015 and 2017 were included in the study. For identification of the isolates, conventional tests and 16S rDNA sequence analysis were performed. Antibiotic susceptibilities of the isolates were determined by Etest. The isolates identified as T. otitidis with VITEK 2 Compact could not be identified by MALDI-TOF MS and described as C. mucifaciens/Corynebacterium afermentans spp. by 16S rDNA sequence analysis. One isolate identified as C. mucifaciens by MALDI-TOF MS could not be identified with VITEK 2 Compact and described as C. mucifaciens by 16S rDNA sequence analysis and conventional methods. All isolates (n:45) described as C. mucifaciens/C. afermentans spp. by 16S rDNA sequence analysis were identified as C. afermentans subsp. afermentans with conventional methods. All 45 isolates identified as C. afermentans subsp. afermentans were resistant to penicillin, erythromycin, and clindamycin and were susceptible to vancomycin and daptomycin, whereas 31 (69%) were resistant to trimethoprim-sulfamethoxazole (TMP-SXT). The isolate identified as C. mucifaciens was susceptible to penicillin, vancomycin, daptomycin, and TMP-SXT; it was resistant to erythromycin and clindamycin. In this study, we reported 45 C. afermentans isolates misidentified as T. otitidis in routine laboratory processes. To our knowledge, this is the first study to include the highest number of C. afermentans blood isolates.
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    Evaluation of the Diagnostic Performance of Xpert MTB/RIF Test for the Detection of Mycobacterium tuberculosis and Rifampin Resistance in Clinical Samples
    (Ankara mıcrobıology soc, hacetlepe unıv faculty medıcıne dept mıcrobıology, 06100 ankara, turkey, 2016) Gursoy, Nafia Canan; Yakupogullari, Yusuf; Tekerekoglu, Mehmet Sait; Otlu, Baris
    Rapid and accurate detection of active tuberculosis (TB) cases is one of the most important goal of tuberculosis control programme. For this purpose, new methods are being developed to isolate, serotype and determine the drug resistance of the agent. Xpert MTB/RIF test (CepheidGeneXpert (R) System, USA) that has been recently developed, is a real-time polymerase chain reaction-based method which detects Mycobacterium tuberculosis complex and resistance of the strain to rifampicin (RIF) from the clinical sample directly within a couple of hours. However, there are not sufficient data about the performance of that test for extrapulmonary samples and pulmonary samples other than sputum. The aims of this study were to investigate the sensitivity, specificity, positive and negative predictive values of Xpert MTB/RIF test in detection of M. tuberculosis and the performance in the determination of rifampicin resistance of the isolates from pulmonary and extrapulmonary clinical samples. A total of 2160 clinical samples, in which 1141 (52.8%) were pulmonary and 1019 (47.2%) were extrapulmonary samples, sent to our laboratory between July 2013 to December 2014, were included in the study. Sixty seven of the evaluated samples (3.1%) were positive with microscopy (acid-fast stain; AFS), 116 samples (5.1%) were positive with culture and 98 samples (4.5%) were positive with Xpert MTB/RIF test. When the culture was considered as the reference method, the sensitivity and specificity of Xpert MTB/RIF test were determined as 73.3% and 99.3%, respectively for all samples; 77.5% and 99.5%, respectively for pulmonary samples and 63.9% and 99.2%, respectively for extrapulmonary samples. Among AFS positive samples, the sensitivity was 100% and specificity was 66.7%; whereas among AFS negative samples those values were 40.4% and 99.4%, respectively. Among all the samples involved in the study, RIF resistance was determined only in three samples with Xpert MTB/RIF test and that was also proved phenotypically (100% concordance). According to mycobacterial culture results, positive and negative predictive values of Xpert MTB/RIF test were determined as 86.7% and 98.5%, respectively for all samples. Those were determined as 92.5% and 98.3%, respectively for extrapulmonary samples and were determined as 74.2 and 98.7%, respectively for pulmonary samples. According to the results obtained in our study, sensitivity of Xpert MTB/RIF test for extrapulmonary samples was found to be at moderate level; sensitivity of the test was found to be decreased especially in AFS negative samples with less bacilli load. Nonetheless, specificity of Xpert MTB/RIF test to the agent in all samples was found to be extremely high. In our study, although RIF-resistant strains were detected in few of the samples, Xpert MTB/RIF test could differentiate all resistant and sensitive strains. Additionally, detection of M. tuberculosis and RIF resistance in our laboratory takes approximately 20.96 days with culture, this period decreases to a couple of hours with Xpert MTB/RIF test. Because of the advantages such as being practical, rapid and requiring minimal safety measures, it was concluded that Xpert MTB/RIF test may contribute to rapid diagnosis of TB also in extrapulmonary samples, with the confirmation of culture method.
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    Evaluation of the Diagnostic Performance of Xpert MTB/RIF Test for the Detection of Mycobacterium tuberculosis and Rifampin Resistance in Clinical Samples
    (Ankara Microbiology Soc, 2016) Gursoy, Nafia Canan; Yakupogullari, Yusuf; Tekerekoglu, Mehmet Sait; Otlu, Baris
    Rapid and accurate detection of active tuberculosis (TB) cases is one of the most important goal of tuberculosis control programme. For this purpose, new methods are being developed to isolate, serotype and determine the drug resistance of the agent. Xpert MTB/RIF test (CepheidGeneXpert (R) System, USA) that has been recently developed, is a real-time polymerase chain reaction-based method which detects Mycobacterium tuberculosis complex and resistance of the strain to rifampicin (RIF) from the clinical sample directly within a couple of hours. However, there are not sufficient data about the performance of that test for extrapulmonary samples and pulmonary samples other than sputum. The aims of this study were to investigate the sensitivity, specificity, positive and negative predictive values of Xpert MTB/RIF test in detection of M. tuberculosis and the performance in the determination of rifampicin resistance of the isolates from pulmonary and extrapulmonary clinical samples. A total of 2160 clinical samples, in which 1141 (52.8%) were pulmonary and 1019 (47.2%) were extrapulmonary samples, sent to our laboratory between July 2013 to December 2014, were included in the study. Sixty seven of the evaluated samples (3.1%) were positive with microscopy (acid-fast stain; AFS), 116 samples (5.1%) were positive with culture and 98 samples (4.5%) were positive with Xpert MTB/RIF test. When the culture was considered as the reference method, the sensitivity and specificity of Xpert MTB/RIF test were determined as 73.3% and 99.3%, respectively for all samples; 77.5% and 99.5%, respectively for pulmonary samples and 63.9% and 99.2%, respectively for extrapulmonary samples. Among AFS positive samples, the sensitivity was 100% and specificity was 66.7%; whereas among AFS negative samples those values were 40.4% and 99.4%, respectively. Among all the samples involved in the study, RIF resistance was determined only in three samples with Xpert MTB/RIF test and that was also proved phenotypically (100% concordance). According to mycobacterial culture results, positive and negative predictive values of Xpert MTB/RIF test were determined as 86.7% and 98.5%, respectively for all samples. Those were determined as 92.5% and 98.3%, respectively for extrapulmonary samples and were determined as 74.2 and 98.7%, respectively for pulmonary samples. According to the results obtained in our study, sensitivity of Xpert MTB/RIF test for extrapulmonary samples was found to be at moderate level; sensitivity of the test was found to be decreased especially in AFS negative samples with less bacilli load. Nonetheless, specificity of Xpert MTB/RIF test to the agent in all samples was found to be extremely high. In our study, although RIF-resistant strains were detected in few of the samples, Xpert MTB/RIF test could differentiate all resistant and sensitive strains. Additionally, detection of M. tuberculosis and RIF resistance in our laboratory takes approximately 20.96 days with culture, this period decreases to a couple of hours with Xpert MTB/RIF test. Because of the advantages such as being practical, rapid and requiring minimal safety measures, it was concluded that Xpert MTB/RIF test may contribute to rapid diagnosis of TB also in extrapulmonary samples, with the confirmation of culture method.
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    Femoral Hemodialysis Catheter-Related Bacteremia Due to Globicatella sanguinis: Challenges in Species Identification
    (Ankara mıcrobıology soc, hacetlepe unıv faculty medıcıne dept mıcrobıology, 06100 ankara, turkey, 2017) Gursoy, Nafia Canan; Sakaci, Tamer
    In this case, catheter-related bacteremia due to Globicatella sanguinis in a 43 years old female patient undergoing hemodialysis with the diagnosis of diabetic nephropathy was presented and the methods in the laboratory diagnosis of the rare opportunistic pathogen, Globicatella cins, were nvestigated. A set of peripheral blood cultures and simultaneous catheter culture was obtained from the patient in third of May 2016. Biochemical tests, Phoenix (Becton Dickinson, USA) and MicroScan (Beckman Coulter, USA) automated systems and matrix assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF MS) based Microflex MS (Bruker, Daltonics, Germany) and VITEK MS (database v2.0) (bioMerieux, France) systems were used for the identification of the cultured bacteria. Partial 16S rDNA sequencing was done by using specific p8FPL 5'-AGT TTG ATC ATG GCT CAG-3' and p806R 5'-GGA CTA CCA GGG TAT CTA AT-3' primers. Minimal inhibitory concentrations (MICs) for vancomycin, erythromycin, imipenem, cefotaxime and benzypenicillin were determined by agar gradient method. The blood and catheter cultures yielded the same type of bacterial colonies. Alfa-hemolytic, catalase negative colonies observed on blood agar plates after an over night incubation yielded gram-positive cocci on Gram staining. In Sisli Hamidiye Etfal Hospital, the isolate was identifed as G. sulfidifaciens (score value > 2) by Bruker MS system and as G. sanguinis by Phoenix automated system. In Inonu University, the isolate could not be identified by Microscan automated system while VITEK MS named the isolate as 99.9% G. sanguinis and 98.3% G. sulfidifaciens. The 16S rDNA sequencing identifed the isolate as 100% G. sanguinis (GenBank accessionno. KJ680157.1). The MIC values were 0.38 mu g/ml, 1.5 mu g/ml, 0.38 mu g/ ml, > 32 mu g/ml and 64 mu g/ml for vancomycin, eryrthromycin, imipenem, cefotaxime and benzylpenicillin, respectively. The patient was diagnosed as catheter-related bacteremia and vancomycin (1 x 1 g IV/72 h) was used for up to 10 days. No fever and bacterial growth in cultures were present in her control visits. As G. sanguinis is not among the commonly encountered pathogens and due to difficulties in laboratory diagnosis, it may be missedor mis-identified in clinical laboratories. BD Phoenix and Bruker MS data bases lack G. sulfidifaciens and G. sanguinis, respectively, while the Globicatella genus is not present in MicroScan database. The increased number of medical implementations and the increasing number of immunosuppressed patient populations in recenty ears will lead to the emergence of rare bacteria. Increasing the diagnostic power of clinical microbiology laboratories by conventional and molecular methods and renewal of the databases of commercial identification systems by expanding the pathogen spectrum are significantly important for the prevention and control of infections caused by these agents.
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    Femoral Hemodialysis Catheter-Related Bacteremia Due to Globicatella sanguinis: Challenges in Species Identification
    (Ankara Microbiology Soc, 2017) Aktas, Elif; Gursoy, Nafia Canan; Sakaci, Tamer; Koc, Yener; Hamidi, Aziz Ahmad; Bulut, Emin; Erdemir, Duygu
    In this case, catheter-related bacteremia due to Globicatella sanguinis in a 43 years old female patient undergoing hemodialysis with the diagnosis of diabetic nephropathy was presented and the methods in the laboratory diagnosis of the rare opportunistic pathogen, Globicatella cins, were nvestigated. A set of peripheral blood cultures and simultaneous catheter culture was obtained from the patient in third of May 2016. Biochemical tests, Phoenix (Becton Dickinson, USA) and MicroScan (Beckman Coulter, USA) automated systems and matrix assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF MS) based Microflex MS (Bruker, Daltonics, Germany) and VITEK MS (database v2.0) (bioMerieux, France) systems were used for the identification of the cultured bacteria. Partial 16S rDNA sequencing was done by using specific p8FPL 5'-AGT TTG ATC ATG GCT CAG-3' and p806R 5'-GGA CTA CCA GGG TAT CTA AT-3' primers. Minimal inhibitory concentrations (MICs) for vancomycin, erythromycin, imipenem, cefotaxime and benzypenicillin were determined by agar gradient method. The blood and catheter cultures yielded the same type of bacterial colonies. Alfa-hemolytic, catalase negative colonies observed on blood agar plates after an over night incubation yielded gram-positive cocci on Gram staining. In Sisli Hamidiye Etfal Hospital, the isolate was identifed as G. sulfidifaciens (score value > 2) by Bruker MS system and as G. sanguinis by Phoenix automated system. In Inonu University, the isolate could not be identified by Microscan automated system while VITEK MS named the isolate as 99.9% G. sanguinis and 98.3% G. sulfidifaciens. The 16S rDNA sequencing identifed the isolate as 100% G. sanguinis (GenBank accessionno. KJ680157.1). The MIC values were 0.38 mu g/ml, 1.5 mu g/ml, 0.38 mu g/ ml, > 32 mu g/ml and 64 mu g/ml for vancomycin, eryrthromycin, imipenem, cefotaxime and benzylpenicillin, respectively. The patient was diagnosed as catheter-related bacteremia and vancomycin (1 x 1 g IV/72 h) was used for up to 10 days. No fever and bacterial growth in cultures were present in her control visits. As G. sanguinis is not among the commonly encountered pathogens and due to difficulties in laboratory diagnosis, it may be missedor mis-identified in clinical laboratories. BD Phoenix and Bruker MS data bases lack G. sulfidifaciens and G. sanguinis, respectively, while the Globicatella genus is not present in MicroScan database. The increased number of medical implementations and the increasing number of immunosuppressed patient populations in recenty ears will lead to the emergence of rare bacteria. Increasing the diagnostic power of clinical microbiology laboratories by conventional and molecular methods and renewal of the databases of commercial identification systems by expanding the pathogen spectrum are significantly important for the prevention and control of infections caused by these agents.
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    Hospital Outbreak of a Colistin-Resistant, NDM-1-and OXA-48-Producing Klebsiella pneumoniae: High Mortality from Pandrug Resistance
    (Mary Ann Liebert, Inc, 2018) Guducuoglu, Huseyin; Gursoy, Nafia Canan; Yakupogullari, Yusuf; Parlak, Mehmet; Karasin, Gokhan; Sunnetcioglu, Mahmut; Otlu, Baris
    Colistin resistance causes substantial problems in the treatment of serious infections with carbapenem-resistant (CR) gram-negative bacteria. In this study, we report a fatal hospital outbreak from the spread of a pandrug-resistant Klebsiella pneumoniae clone. An outbreak investigation was conducted after consecutive isolation of nine CR-K. pneumoniae (CR-Kp) strains from eight patients in two intensive care units of a university hospital within 2 weeks. Carbapenem and colistin resistance genes were investigated with PCR, clonal relationships of isolates were studied with pulse-field gel electrophoresis, and multilocus sequence types were determined. The outcomes of the affected patients were analyzed. Genotyping showed a predominant CR-Kp clone consisting of seven strains from six patients. These strains were in ST11 type, an international high-risk clone. They were resistant to all antimicrobials, including colistin, and positive for NDM-1 and OXA-48 carbapenemases, but negative for plasmid-borne colistin resistance genes. One patient had colonization and the remaining five died due to the infection within mean 12 days. No environmental or staff links could be established, and the outbreak was stopped by augmenting infection-control measures. Colistin-resistant K. pneumoniae could clonally expand in the hospital setting, and this spread might be associated with high mortality due to the lack of an appropriate treatment option. Immediate implementation of infection-control measures may be the best way to limit fatal consequences of the spread of such incurable pathogens.
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    Prevalence of antibiotic-resistant Salmonella in retail organic chicken
    (Emerald Group Publishing Ltd, 2020) Guran, Husnu Sahan; Ciftci, Resat; Gursoy, Nafia Canan; Ozekinci, Tuncer; Alali, Walid Q.
    Purpose The objective of this study was to determine Salmonella prevalence, antimicrobial-resistant phenotypes, and their genetic relatedness in frozen organic chicken collected at retail level in Turkey. Design/methodology/approach Retail packs (n = 348) of cut-up chicken parts (breast, leg quarter and drumstick) and whole chicken carcasses were purchased from a central hypermarket in Diyarbakir (Southeast Anatolia Region in Turkey) and from a large online retailer in Turkey. The retail packs were paired by part type, brand, production date, and sell-by date. The chicken samples were analyzed for the presence of Salmonella spp., and then isolates were screened for antibiotic susceptibility, class I integron, and genetic similarity. Findings Salmonella prevalence in retail frozen organic chicken samples was 6.3 percent; however, the prevalence by parts, leg quarter, drumstick, breast, and whole chicken was 2.1 percent, 10.4 percent, 10.4 percent, and 0 percent, respectively. Salmonella prevalence was significantly higher in samples obtained from the hypermarket (9.2 percent) compared to online retailer (3.8 percent). All the isolates were serotype Infantis, genetically similar (highly clonal), and 68.2 percent harbored class I integron. All isolates were resistant to ciprofloxacin (drug of choice to treat salmonellosis in human), and 86.3 percent of the isolates were multidrug-resistant. Originality/value Salmonella prevalence in organic chicken meat, regardless of the retail market source in Turkey, may pose a health risk to consumers especially with the high prevalence of multi-drug resistant phenotypes. Findings inform researchers and the public about the safety of organically produced chicken and the potential health risk to consumers.
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    Prevalence of Various Sexually Transmitted Pathogens in Infertile Couples and Their Effects on In Vitro Fertilization Success
    (Bilimsel Tip Yayinevi, 2019) Gursoy, Nafia Canan; Tuncay, Gorkem; Karaer, Abdullah; Tecellioglu, Ayse Nihan; Yigit, Hande; Yakupogullari, Yusuf; Otlu, Baris
    Introduction: Sexually transmitted infections can cause problems such as infertility, ectopic pregnancy and miscarriage due to tissue damage and function loss in female and male genital system. Although the role of bacterial agents in the etiopathogenesis of infertility is well known, the association of some viral agents that can be transmitted by sexual intercourse with infertility is relatively little known. The aim of this study was to investigate the presence of cytomegalovirus (CMV), human papillomavirus (HPV), herpes simplex virus (HSV 1 and HSV 2), human immunodeficiency virus (HIV), hepatitis B virus (HBV), hepatitis C virus (HCV) and Chiamydia trachomatis in infertile patients undergoing in vitro fertilization (IVF) treatment. Materials and Methods: Semen and cervical samples were taken from 149 infertile couples during one year, and the presence of agents was investigated with polymerase chain reaction (PCR). The effect of these agents on in vitro fertilization (IVF) treatment success was evaluated. Results: It was found that 8.1% (12/149) of the 149 infertile couples receiving IVF treatments were infected with CMV, and CMV-DNA positivity was found as 2% (3/149) in sperm samples and 6% (9/149) in cervical samples. CMV infection was not observed in both pairs. HPV infection was observed in 9.4% (14/149) of the couples and HPV-DNA was found to be 5.4% (8/149) in sperm samples and 7.4% (11/149) in cervical samples. The oncogenic high-risk HPV (HR-HPV) genotype ratio in sperm samples was 37.5% (3/8) and the most common genotypes were HPV 18, 35 and 39, respectively. HR-HPV genotype ratio in cervical samples was 63.6% (7/11) and HPV 35, 16, 18, 45 and 53 were the most common genotypes. While HPV-DNA was found to be positive in 3.4% (5/149) in both of the pairs, interpair consistency was 40% (2/5) for HPV genotypes. HPV or CMV positivity did not have a statistically significant effect on sperm parameters, number of oocytes, embryos, and clinical pregnancy and live birth rate after IVF. C. trachomatis, HSV-1/2, HBV, HCV and HIV viruses were not detected in any of the sperm and cervical samples of the couples. Conclusion: More extensive studies are needed to investigate the possible agents in infertile patients. In order to eliminate the lack of epidemiological data on this subject, it would be useful to give priority to patients admitted to IVF clinics that are easier to reach first and to bacterial/viral factors that are seen endemic in our country, especially.
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    A RARELY ISOLATED GRAM- NEGATIVE BACTERIUM IN MICROBIOLOGY LABORATORIES: LECLERCIA ADECARBOXYLATA
    (Akademiai Kiado Zrt, 2018) Cicek, Muharrem; Tuncer, Ozlem; Bicakcigil, Asiye; Gursoy, Nafia Canan; Otlu, Baris; Sancak, Banu
    [Abstract Not Available]
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    A rarely ısolated gram- negatıve bacterıum ın mıcrobıology laboratorıes: leclercıaadecarboxylata
    (Akademıaı kıado zrt, budafokı ut 187-189-a-3, h-1117 budapest, hungary, 2018) Cicek, Muharrem; Tuncer, Ozlem; Bicakcigil, Asiye; Gursoy, Nafia Canan; Otlu, Baris; Sancak, Banu
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    The Research of Clonal Relationship Among Aeromonas Strains Isolated from Human, Animal and Drinking Water by PFGE
    (Kafkas Univ, Veteriner Fakultesi Dergisi, 2013) Korkoca, Hanifi; Berktas, Mustafa; Durmaz, Riza; Gursoy, Nafia Canan
    Aeromonads infect human through potable water and causes various infections. Their existence in animal are being assessed as potential risk for human health. The aim of this study was to investigate clonal relationship among 52 Aeromonas strains isolated from human with diarrhea (14 strains), healthy food workers (2 strains), animals (24 strains) and drinking water (12 strains) by pulsed-field gel electrophoresis (PFGE). Clonal relation was determined between one diarrheic human isolate and one cattle isolate. No clonal relation was determined between drinking water and human isolates. Two fish isolates, A. caviae and A. sobria, were not distinguished PFGE patterns. Consequently no predominant clone was determined while clonal related strains were determined. Particularly, it is necessary to elicit the epidemiological importance of animals in respect of human Aeromonas infections and extensive studies are required for identification of environmental isolates.
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    A retrospective observational cohort study of the clinical epidemiology of bloodstream infections due to carbapenem-resistant Klebsiella pneumoniae in an OXA-48 endemic setting
    (Elsevier, 2022) Aslan, Abdullah Tarik; Kirbas, Ekin; Sancak, Banu; Tanriverdi, Elif Seren; Otlu, Baris; Gursoy, Nafia Canan; Yilmaz, Yakut Akyon
    This study aimed to characterize the epidemiology and clinical outcomes of patients with bloodstream infections (BSIs) due to carbapenem-resistant Klebsiella pneumoniae (CRKP) in an OXA-48-predominant environment. This was a retrospective single-centre cohort study including all consecutive patients with CRKP BSIs treated between 1 January 2014 and 31 December 2018. Multivariate analysis, subgroup analysis and propensity-score-matched analysis were employed to analyse 30-day mortality as the primary outcome. Clinical cure at day 14 was also analysed for the whole cohort. In total, 124 patients with unique isolates met all the inclusion criteria. OXA-48 was the most common type of carbapenemase (85.5%). Inappropriate therapy was significantly associated with 30-day mortality [70.6% vs 39.7%, adjusted odds ratio (aOR) 4.65, 95% confidence interval (CI) 1.50-14.40, P=0.008] and 14-day clinical failure (78.5% vs 56.2%, aOR 3.14, 95% CI 1.09-9.02, P=0.033) in multivariate analyses. Among those treated appropriately, the 30-day mortality rates were similar in monotherapy and combination therapy arms (OR 2.85, 95% CI 0.68-11.95, P=0.15). INCREMENT CPE mortality score (aOR 1.16, 95% CI 1.01-1.33, P=0.029), sepsis at BSI onset (aOR 2.90, 95% CI 1.02-8.27, P=0.046), and inappropriate therapy (aOR 4.65, 95% CI 1.50-14.40, P= 0.008) were identified as independent risk factors for 30-day mortality. Colistin resistance in CRKP had no significant impact on 30-day mortality. These results were also confirmed in all propensity-scorematched analyses and sensitivity analyses. Appropriate regimens were associated with better clinical outcomes than inappropriate therapies for BSIs with CRKP predominantly possessing OXA-48. (c) 2022 Elsevier Ltd and International Society of Antimicrobial Chemotherapy. All rights reserved.
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    A silent outbreak due to Klebsiella pneumoniae that co-produced NDM-1 and OXA-48 carbapenemases, and infection control measures
    (Mashhad Univ Med Sciences, 2020) Duman, Yucel; Ersoy, Yasemin; Gursoy, Nafia Canan; Toplu, Sibel Altunisik; Otlu, Baris
    Objective(s): ): Infections due to carbapenemase-producing Klebsiella pneumoniae are associated with high morbidity and mortality. In this study, we report a hospital outbreak due to co-producing OXA-48 and NDM-1 K. pneumoniae clone. The aim of the study is to investigate the clonal relationship of strains, risk factors of outbreak and infection control measures. Materials and Methods: Once an outbreak was suspected at the end of December 2017 in our intensive care unit (ICU), carbapenem resistance K. pneumoniae identified in patients' specimens. An outbreak analysis was begun to determine the risk factors and dissemination of the cases. A case-control study was conducted to determine the risk factors. To control the outbreak; tight contact prevention, good clean-up the medical devices and hospital environment, were done. Staff training programs such as hand hygiene, disinfection, wearing aprons, good cleaning were created. Carbapenem resistance genes determined by PCR. Clonal relationships of strains investigated by PFGE. Results: We investigate 21 carbapenem-resistant K. pneumonia strains. Nine of them were found co-produced NDM-1 and OXA-48, 11 strains produced OXA-48, and one strain produced NDM-1. Seven strains of co-producing NDM-1 and OXA-48 were found clonally related with PFGE. We could not determine any risk factor except rectal colonization in the case-control study. Conclusion: The interventions that successfully controlled this outbreak were hand hygiene, tight contact prevention, good clean-up of the hospital environment and medical devices. As a result, we believe that it would be beneficial to take infection control measures to prevent the spread of these strains to the community and hospital settings.
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    Systemic nocardiosis in a dog caused by Nocardia cyriacigeorgica
    (Biomed Central Ltd, 2017) Eroksuz, Yesari; Gursoy, Nafia Canan; Karapinar, Tolga; Karabulut, Burak; Incili, Canan Akdeniz; Yerlikaya, Zeynep; Toraman, Zulal Asci
    Background: Systemic nocardiosis due to Nocardia cyriacigeorgica has not been reported in dogs. Case presentation: Light and electron microscopy, microbiological culture and molecular identification (PCR) were used to diagnose systemic nocardiosis caused by Nocardia cyriacigeorgica in a 3-month-old husky dog. The postmortem changes included multifocal to coalescing, sharply circumscribed pyogranulomatous inflammation and abscess formation in lungs, liver, myocardium, spleen, kidneys, brain, and hilar lymph nodes. The organism was isolated and sequencing of its 16S rRNA allowed its identification and speciation. Examination of the bacterial culture by scanning electron-microscope showed filamentous branching with fragmentation into widely bacillary and cocoid forms of the bacteria. There was no history of immunosupressive drug administration and infection by the immunosuppresive viral pathogens, canine distemper and parvovirus were excluded via PCR. Conclusion: N. cyriacigeorgica should be considered potential cause of systemic pyogranulomatous lesions in dogs. It is the first reported case of systemic nocardiosis due to N. cyriacigeorgica in a dog.