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Öğe Anti-lipoxygenase Evaluation of Different Achillea sp. Extracts(Wiley-V C H Verlag Gmbh, 2025) Subasi, Bilgen; Gunbatan, Tugba; Gurbuez, Ilhan; Inal, Ebrar; Kartal, Murat; Sucu, Melike; Icen, Mehmet SinaIn this present work, in vitro lipoxygenase (LOX) inhibitory activities of methanol extracts and different fractions of six Achillea species [A. biebersteinii, A. millefolium, A. cappadocica, A. wilhelmsii, A. setacea, and A. nobilis subsp. neilreichii] were evaluated. A microplate-based spectrophotometric method was utilized for the LOX assay. The phenolic composition was analyzed using the liquid chromatography-high resolution mass spectrometer (LC-HRMS). As a result, the A. biebersteinii (Malatya locality) ethyl acetate fraction showed the highest lipoxygenase inhibitory activity (72.9% inhibition), followed by the A. cappadocica ethyl acetate fractions with 66.9% and 60.4% inhibition, respectively. The phytochemical LC-HRMS analyses revealed that the active constituents of the studied fractions were vanillic acid, hyperoside, p-coumaric acid, quercetin, and chrysoeriol. The findings showed the active bioassay-directed fractions, as well compounds to the best of our knowledge for the first time, suggesting further future biological, pharmacological, and toxicological evaluations both in vitro and in vivo.Öğe Hidden Ethnomedicinal Diversity in a Fine-Scale Study from Konak, Eastern Anatolia(Mdpi, 2026) Kolac, Turgay; Sadikoglu, Narin; Icen, Mehmet SinaThis study documents the ethnomedicinal knowledge of Konak (Malatya, Eastern Anatolia, T & uuml;rkiye), a region with rich plant diversity but no prior comprehensive research. The aim of the study is to systematically document and analyze the ethnomedicinal practices of Konak village, focusing on plant taxa (species, subspecies and varieties) used, preparation methods, and therapeutic applications. Data were collected through semi-structured interviews with 68 local informants. Quantitative analysis was performed using Informant Consensus Factor (FIC) and Use Value (UV) indices. Plant specimens were collected, identified, and deposited in the herbarium. The study documented 86 plant taxa from 35 families used in 230 therapeutic applications. Lamiaceae, Asteraceae, and Rosaceae were the most represented families. High FIC values were recorded for colds (FIC = 0.95), stomach pain (FIC = 0.92), and inflammation (FIC = 0.90), indicating strong community consensus. The most frequently cited species were Origanum vulgare subsp. gracile, Mentha spp., and Rosa canina. There are novel or locally specific uses, with 13 taxa having no previously recorded ethnomedicinal applications in the reviewed literature. The findings reveal Konak as a significant repository of ethnomedicinal knowledge. High-FIC taxa represent prime candidates for phytochemical and pharmacological research to validate traditional uses and support evidence-based phytotherapy. This study enriches regional ethnopharmacological data and highlights candidate taxa for pharmacological validation.Öğe Isolation of rosmarinic acid and methyl rosmarinate as lipoxygenase inhibitors from Salvia palaestina Benth. by activity-guided fractionation(Elsevier, 2021) Icen, Mehmet Sina; Gurbuz, Ilhan; Bedir, Erdal; Gunbatan, Tugba; Demirci, FatihSalvia palaestina aqueous and methanol extracts were prepared from the aerial parts, which were evaluated for the in vitro anti-inflammatory properties using the lipoxygenase (LO) enzyme inhibition assay. While the aqueous extract showed no activity at test concentrations, a significant (p < 0.001) lipoxygenase inhibition was detected for the methanol extract with 29% inhibition. Activity guided fractionation was carried out on the methanol extract via liquid-liquid partitioning using n-hexane, dichloromethane, ethyl acetate, and n-butanol. The ethyl acetate fraction showed statistically the best inhibition among the sub-fractions with 70% inhibition (p < 0.0001). The compounds responsible for the activity were purified, and their structures were established as rosmarinic acid, and methyl rosmarinate by spectroscopic methods. IC50 values of rosmarinic acid, and methyl rosmarinate were calculated as 0.21 and 0.02 mu M, respectively. In conclusion, the in vitro anti-inflammatory potential of S. palaestina was associated to rosmarinic acid, and methyl rosmarinate, for the first time to the best of our knowledge. (C) 2021 SAAB. Published by Elsevier B.V. All rights reserved.Öğe Preventive effects of systemic Pistacia eurycarpa Yalt. administration on alveolar bone loss and oxidative stress in rats with experimental periodontitis(Univ Sao Paulo Fac Odontologia Bauru, 2024) Atalay, Mustafa; Uslu, Mustafa Ozay; Icen, Mehmet Sina; Uremis, Nuray; Turkoz, YusufObjective: This study aimed to investigate the effects of systemic administration of P. eurycarpa Yalt. plant extract on alveolar bone loss and oxidative stress biomarkers in gingival tissue in a rat model of experimental periodontitis. Methodology: 32 male Wistar albino rats, weighing 200-250 g, were divided into four groups (n=8): Healthy control (HC), Experimental periodontitis control (EPC), Experimental periodontitis 400 mg/kg (EP400), Experimental periodontitis 800 mg/kg (EP800). Experimental periodontitis was induced using the ligating method. Distilled water was administered to the HC and EPC groups and the plant extract was administered to the EP400 and EP800 groups by oral gavage at doses of 400 mg/kg and 800 mg/kg, respectively. The rats were sacrificed on the 15th day. The values of glutathione peroxidase GSH-Px, malondialdehyde (MDA), superoxide dismustase (SOD), interleukin-113 (IL-113), interleukin-10 (IL-10), total antioxidant status (TAS), total oxidant status (TOS), oxidative stress index (OSI) in the gingival tissues were analyzed by ELISA tests. Alveolar bone loss was assessed using micro-CT images of the maxilla. Results: Although the IL-113, TOS, OSI results of the healthy control group were lower than those of the other groups, the TAS values were higher (p<0.05). No significant difference was found in the biochemical parameters among the EPC, EP400, and EP800 groups (p>0.05). Alveolar bone loss was significantly reduced in the extract groups compared to the EPC group (p<0.001). Conclusion: Within the limitations of this study, it was observed that the systemic P. eurycarpa extract application reduced alveolar bone loss in a rat model of experimental periodontitis. Further studies are needed to elucidate the beneficial effects
