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    Evaluation of biological activities of Barbarea integrifolia and isolation of a new glucosinolate derivated compound
    (Walter De Gruyter Gmbh, 2021) Badem, Merve; Sener, Sila Ozlem; Kanbolat, Seyda; Korkmaz, Nuriye; Yildirmis, Sermet; Ozgen, Ufuk; Aliyazicioglu, Rezzan
    The aim of the present study is to determine the potent biological activities and carry out isolation studies on Barbarea integrifolia. The antioxidant capacity of the species was evaluated by total phenolic content, FRAP, CUPRAC, and DPPH radical scavenging activity. Anticancer activity studies were performed by MTT assay in MDA-MB-231, MCF-7, Hep3B, PC-3, A549, HCT116, L-929 cell lines. It was observed that the remaining aqueous fraction has higher total phenolic content while higher activity in the CUPRAC and FRAP assays was displayed for the methanolic extract and chloroform fraction. The extracts showed anticancer activity as compared with vincristine. It was observed that chloroform fraction has the highest anticancer activity on MCF-7 cell line, while ethyl acetate fraction has the highest anticancer activity on Hep-3B and A549 cell lines. Methanolic extract has the highest anticancer activity on HCT116 and MDA-MB-23 cell lines. The isolation studies have been performed using several chromatographic methods. The chemical structures of compounds have been identified by means of H-1 NMR, C-13 NMR, 2D-NMR, and MS. Five major compounds, one steroid (beta-Sitosterol), one phenolic acid (Rosmarinic acid), one flavonol heteroside (kaempferol 7-O-alpha-L-rhamnoside-3-O-beta-D-(2-O-beta-D-glucosyl)-beta-D-glucoside), and two glucosinolates (Gluconasturtiin, Gluconasturtiin choline salt) have been isolated.
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    Investigation of therapeutic potential of three endemic Cirsium species for global health problem obesity
    (Elsevier, 2021) Sener, Sila Ozlem; Ozgen, Ufuk; Kanbolat, Seyda; Korkmaz, Nuriye; Badem, Merve; Hanci, Hatice; Dirmenci, Tuncay
    Obesity is a risk factor for metabolic disorders causes serious health problems and economic costs globally. Some Cirsium genus has been reported to possess anti-obesity effect and traditional uses for vascular diseases, anti-inflammatory and diuretic effects. This study aims to investigate the therapeutic value of endemic Cirsium species for obesity treatment. C. balikesirense Yildiz, Arabaci & Dirmenci (CB), C. nerimaniae Yildiz, Dirmenci & Arabaci (CN) and C. sommieri Petr. (CS) were selected for the study. High-fat diet (HFD) induced obese Sprague Dawley rats were employed for in vivo studies. The extract obtained aerial parts of Cirsium species were applied to rats orally for 8 weeks. To interpret the antiobesity activity, body weight of the rats were measured. Also, biochemical, histopathological and quantitative real-time PCR (RT-PCR) analysis were performed on rats. Bioactivity-guided fractionation studies were carried out guidance with in vitro lipase enzyme inhibition. The structures of the pure compounds purified using various chromatographic techniques were determined by spectroscopic methods. Oral administration of the extracts and orlistat decreased in body weights, adipocytes size, levels of serum low-density lipoprotein (LDL), triglyceride (TG), leptin levels, lipase, tumor necrosis factor-alpha (TNF-alpha), interleukin-1 beta (IL-1 beta), and increased levels of serum high-density lipoprotein (HDL), adiponectin. According to RT-PCR analysis, the extracts reduced the levels of key transcription factors in adipogenesis and fatty acid synthase (FAS). Bioactivity-guided fractionation studies led to isolation of active compounds as syringin, cimidahurinin, astragalin, afzelin, lupeol, and psi-taraxasterol. In conclusion, CB, CN, CS and their isolated compounds were uncovered to be hopeful as a candidate for antiobesity therapeutics. (C) 2021 SAAB. Published by Elsevier B.V. All rights reserved.