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Öğe Antidepressant effect of Gentiana olivieri Griseb. in male rats exposed to chronic mild stress(Taylor & Francis Ltd, 2020) Berk, Ahmet; Yllmaz, Ismet; Abacioglu, Nurettin; Kaymaz, Mustafa Bahadir; Karaaslan, Merve Goksin; Savan, Ebru KuyumcuBackground: The flowering parts of Gentiana olivieri, known as 'Afat' in the southeastern Anatolia region of Turkey, are used as a tonic, an appetizer, and for the treatment of several mental disorders, including depression. The purpose of this study is to investigate the antidepressant effect of G. olivieri ethanol extract (GOEE) in a chronic mild stress-induced rat model, which was used to mimic a depressive state in humans, and to compare the effect with that of imipramine. Methods: Male Sprague-Dawley rats were randomly divided into six groups: control, stress, treated with imipramine (positive control) and treated with GOEE at three different (200, 500, 1000 mg/kg) doses groups. The rats in all groups, except the control group, were exposed to chronic mild stress. At the end of the 3-week experimental period, biochemical and behavioral parameters were examined. Results: The results showed that treatment with GOEE or imipramine significantly improved rats' sucrose consumption which was diminished by chronic mild stress, restored serum levels of corticosterone and proinflammatory cytokines (interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-alpha)), prevented the increase of liver index of rats. Moreover, in the hippocampus tissue, decreased serotonin and noradrenaline levels were significantly increased by treatment with GOEE or imipramine, and antioxidant parameters (thiobarbituric acid reactive substances (TBARS), superoxide dismutase (SOD), and glutathione (GSH)) were significantly improved by treatment with GOEE though not with imipramine. Conclusion: The data demonstrate that G. olivieri may exert its antidepressant activity by improving monoaminergic system disorders, and by favorably affecting the antioxidant, inflammatory and the endocrine mechanisms.Öğe Biodegradable non-aromatic adhesive polyurethanes based on disaccharides for medical applications(Elsevier Sci Ltd, 2014) Ates, Burhan; Koytepe, Suleyman; Karaaslan, Merve Goksin; Balcioglu, Sevgi; Gulgen, SelamThis work addresses synthesis, characterization, adhesive properties, protein adsorption and in vitro biodegradation of non-aromatic polyurethanes (NPUs). The following reactants were used in the syntheses of polyurethanes (PUs): non-aromatic 4,4'-Methylenebis(cyclohexyl isocyanate) (MCI), disaccharides (maltose and sucrose) and polyethylene glycol 200 (PEG 200). The NPUs exhibited low glass transition temperature (T-g) and good thermal stability. The dynamic contact angles on NPUs were influenced by the ratios of PEG 200-disaccharide monomer and the hard segment of the NPUs. The highest adhesion strength in whole polyurethanes was found as 307.6 +/- 50.1 kPa for 15% sucrose containing polyurethane. Protein adsorptions of polyurethane films were measured as average 10 pg Bovine Serum Albumin (BSA)/cm(2) and 5 mu g fibrinogen/cm(2) for maltose, and 15 mu g BSA/cm(2) and 5 mu g fibrinogen/cm(2) for sucrose. Biodegradability ratios of NPUs were in the range of 15-35% for 8 weeks depending on the disaccharides containing NPUs. (C) 2013 Elsevier Ltd. All rights reserved.Öğe Biomimetic approach to tunable adhesion of polyurethane adhesives through Fe3+ crosslinking and hydrophobic tween units with balance of adhesion/cohesion forces(Elsevier Sci Ltd, 2019) Ates, Burhan; Koytepe, Suleyman; Balcioglu, Sevgi; Karaaslan, Merve Goksin; Kelestemur, Unzile; Gulgen, Selam; Ozhan, OnuralBiocompatible adhesives have some limitations such as weak adhesion and low flexibility. To overcome these limitations, we described multiple strategies to provide strong adhesion and high flexibility through Tweens, chlorogenic acid (CLA) and polyethylene glycol (PEG) by reducing excessive interaction between tissue and the adhesive. We synthesized polyurethane-based adhesives using aliphatic 4,4'-methylenebis(cyclohexyl isocyanate) (HMDI), PEG, CLA and Tween units. Hydrophobic side chains in polymer resulted in lower Tg (- 36.95-30.36 degrees C) which indicated more flexibility. The highest adhesion strengths were found as almost 346 kPa for bare polyurethane and 492 kPa for chelated polymer (PU-T40-CLA-15% (5% Tween 40, 15% chlorogenic acid and 80% PEG 200 containing polymer)) with FeCl3. The addition of Tween units provided more stable structure to polymers which proved with in vitro erosion studies. Relatively low erosion values were seen as 5.7, 5.6 and 8.2% in PU-T40-CLA-5% (15% Tween 40, 5% chlorogenic acid and 80% PEG 200 containing polymer), PU-T40-CLA-10% (10% Tween 40, 10% chlorogenic acid and 80% PEG 200 containing polymer), and PU-T40-CLA-15% (5% Tween 40, 15% chlorogenic acid and 80% PEG 200 containing polymer), respectively. In vitro biocompatibility results showed high cell viability in PU-T40-15% as more than 100%. Overall, our findings indicated that these material designs (PU-T-CLAs) provided to overcome the significant challenges of tissue adhesives by improving the flexible character and adhesive strength of the adhesives.Öğe Characterization and evaluation of the antimicrobial properties of algal alginate; a potential natural protective for cosmetics(Marmara Univ, 2022) Sayin, Selin; Depci, Tolga; Naz, Mehmet; Sezer, Selda; Karaaslan, Merve Goksin; Aras, Aycan; Ugur, SinemSargassurn vulgare was sampled by free dives in Iskenderun Bay, Hatay, Turkey, in September 2018. Sargassum vulgare is a material with high economic value because it has compounds that can be used in medical applications such as alginic acid and at the same time it contains carbohydrates and vitamins. Alginates were extracted with a sequential extraction protocol from Sargassum vulgare. Structural characterization of alginate obtained from Sargassum vulgare was determined by FT-IR spectrum, phase structure by XRD diffractometer, and surface morphology by SEM image. Within the scope of the study, alginate obtained from Sargassurn vulgare and herbal preservative 705 used in the field of cosmetics were compared. After pretreatment of Sargassurn vulgare with ethanol, alginate extraction was performed. Microorganisms of Pseudontonas aeruginosa, Staphylococcus aureus, Candida albicans, Escherichia soli, Aspergillus brasiliensis were used to examine the antimicrobial activity of the obtained alginate and showed that the contamination risk was tolerable for all microorganisms examined on the seventh day of incubation. Alginate obtained from Sargassum vulgare was found to be more effective than herbal preservative 705 at 0 hours. Pseudomonas aeruginosa, Staphylococcus aureus are microorganisms with the highest effect in the 0 hour. Since alginate obtained from Sargassurn vulgare is more effective on microorganisms in a shorter time than herbal preservative 705, it is predicted that it can be a product that can be used in the field of cosmetics.Öğe Chemical composition, antimicrobial, insecticidal, phytotoxic and antioxidant activities of Mediterranean Pinus brutia and Pinus pinea resin essential oils(Chinese Journal Natural Medicines, 2014) Ulukanli, Zeynep; Karaborklu, Salih; Bozok, Fuat; Ates, Burhan; Erdogan, Selim; Cenet, Menderes; Karaaslan, Merve GoksinEssential oils of the resins of Pinus brutia and Pinus pinea were evaluated for their biological potential. Essential oils were characterized using GC-MS and GC/FID. In vitro antimicrobial, phytotoxic, antioxidant, and insecticidal activities were carried out using the direct contact and the fumigant assays, respectively. The chemical profile of the essential oils of the resins of P. pinea and P. brutia included mainly a-pinene (21.39% and 25.40%), beta-pinene (9.68% and 9.69%), and caryophyllene (9.12% and 4.81%). The essential oils of P. pinea and P. brutia exerted notable antimicrobial activities on Micrococcus luteus and Bacillus subtilis, insecticidal activities on Ephestia kuehniella eggs, phytotoxic activities on Lactuca sativa, Lepidium sativum, and Portulaca oleracea, as well as antioxidant potential. Indications of the biological activities of the essential oils suggest their use in the formulation of ecofriendly and biocompatible pharmaceuticals.Öğe Chemistry, structure, and biological roles of Au-NHC complexes as TrxR inhibitors(Academic Press Inc Elsevier Science, 2020) Karaaslan, Merve Goksin; Aktas, Aydin; Gurses, Canbolat; Gok, Yetkin; Ates, BurhanIn recent years, the preparation of metal complexes and the introduction of biologically active organometalic compounds are new strategies in drug development. For this purpose, generally N-heterocyclic pharmaceutical agents have been used as promising nuclei. Au-containing N-heterocyclic carbene (Au-NHC) derivatives are among the compounds used for this purpose, and their enzyme inhibition, antioxidant activity, antimicrobial and anticancer properties are widely studied. In these studies, the anticancer property of Au-NHC complexes is the most widely studied area. The common result in different studies has been revealed that mitochondrial thioredoxin reductases (TrxR) inhibition is the main pathway in the powerful anticancer effect of many Au-NHC complexes. In TrxR inhibition, the high affinity of gold to sulfur is considered to be the main component of the effect. This review includes the discussions releated to the anticancer activities and TrxR inhibition properties of Au-NHC compounds.Öğe Chlorogenic Acid Containing Bioinspired Polyurethanes: Biodegradable Medical Adhesive Materials(Taylor & Francis As, 2015) Ates, Burhan; Koytepe, Suleyman; Karaaslan, Merve Goksin; Balcioglu, Sevgi; Gulgen, Selam; Demirbilek, Murat; Denkbas, Emir BakiHighly adhesive bioinspired polyurethanes (PUs) based on the chlorogenic acid (CLA) were prepared from 4,4'-methylenebis (cyclohexyl isocyanate) (MCI) and polyethylene glycol 200 (PEG 200). The polyurethanes exhibited the lowest glass transition temperature (T-g), improved thermal stability and good adhesive properties. The highest adhesion strength was found as 373.3 +/- 47.5 kPa for 10% CLA containing PUs. The polyurethanes were observed to be biodegradable and were in the range of 19-24% for 8 weeks as depending on chlorogenic acid containing of PUs. As a result, prepared biocompatible-adhesive bioinspired polyurethanes are good candidates for medical applications as a tissue adhesive material. [GRAPHICS] .Öğe Cytotoxicity, Radical Scavenging, Antioxidant Properties and Chemical Composition of the Essential Oil of Satureja cilicica P.H. Davis from Turkey(Marmara Univ, Fac Pharmacy, 2017) Arabaci, Turan; Uzay, Gizem; Kelestemur, Unzile; Karaaslan, Merve Goksin; Balcioglu, Sevgi; Ates, BurhanSatureja cilicica P. H. Davis is an endemic species of Lamiaceae, distributed in Turkey. In this study, the essential oil of S. cilicica was obtained by hydrodistillation from the aerial parts. The essential oil was analyzed by GC/MS and the main constituents are identified. Radical scavenging capacities of oil obtained from S. cilicica were determined by using DPPH and ABTS radical scavenging assays. In addition, ferric-reducing power of the essential oil was detected. Cytotoxic activity of the essential oil was determined by MTT assay after 24 h treatment of MCF-7. The major components of the essential oil are p-cymene (17.68%), carvacrol (14.02%), gamma-terpinene (11.23 %) and thymol (8.76%). Radical scavenging capacities of the essential oil were measured as 3.28 +/- 0.02 and 238.15 +/- 3.59 mg Trolox equivalent per milliliter of oil in term of DPPH and ABTS radical scavenging, respectively. Ferric-reducing power of essential oil was found 39.76 +/- 3.66 mg Trolox equivalent per milliliter of essential oil. Growth inhibition effect of essential oil against MCF-7 cancer cell is established. According to the results, essential oil of S. cilicica exhibit low cytotoxic activity, with IC50 values, with IC50 value of 268 mu g/mL. The findings of the analyzed species are compared with the results of previous studies.Öğe Design of Xylose-Based Semisynthetic Polyurethane Tissue Adhesives with Enhanced Bioactivity Properties(Amer Chemical Soc, 2016) Balcioglu, Sevgi; Parlakpinar, Hakan; Vardi, Nigar; Denkbas, Emir Baki; Karaaslan, Merve Goksin; Gulgen, Selam; Taslidere, ElifDeveloping biocompatible tissue adhesives with high adhesion properties is a highly desired goal of the tissue engineering due to adverse effects of the sutures. Therefore, our work involves synthesis, characterization, adhesion properties, protein adsorption, in vitro biodegradation, in vitro and in vivo biocompatibility properties of xylose-based semisynthetic polyurethane (NPU-PEG-X) bioadhesives. Xylose-based semisynthetic polyurethanes were developed by the reaction among 4,4'-methylenebis(cyclohexyl isocyanate) (MCI), xylose and polyethylene glycol 200 (PEG). Synthesized polyurethanes (PUs) showed good thermal stability and high adhesion strength. The highest values in adhesion strength were measured as 415.0 +/- 48.8 and 94.0 +/- 2.8 kPa for aluminum substrate and muscle tissue in 15% xylose containing PUs (NPU-PEG-X-15%), respectively. The biodegradation of NPU-PEG-X-15% was also determined as 19.96 +/- 1.04% after 8 weeks of incubation. Relative cell viability of xylose containing PU was above 86%. Moreover, 10% xylose containing NPU-PEG-X (NPU-PEG-X-10%) sample has favorable tissue response, and inflammatory reaction between 1 and 6 weeks implantation period. With high adhesiveness and biocompatibility properties, NPU-PEG-X can be used in the medical field as supporting materials for preventing the fluid leakage after abdominal surgery or wound closure.Öğe Determination of antioxidant capacity using different acidified solvents and element contents of Allium tuncelianum: A regional and varietal study on endemic edible garlic(Taylor & Francis Inc, 2019) Karaaslan, Nagihan M.; Ince, Muharrem; Ince, Olcay Kaplan; Karaaslan, Merve GoksinAllium tuncelianum (Tunceli garlic), an endemic and threatened plant species in the Tunceli province of Eastern Turkey, is an important native source for local people. The antioxidant activities of the acidified extracts of water, acetonitrile, methanol, and ethanol of Allium tuncelianum were investigated using five antioxidant capacity tests. The results indicated that methanol extract efficiencies of garlic samples using the ABTS, CUPRAC, and reducing power capacities were higher than the other extracts. Water extract and acetonitrile extract values were highest for total phenolic content and metal chelating activities, respectively. Moreover, the element contents such as calcium (Ca), copper (Cu), iron (Fe), potassium (K), magnesium (Mg), manganese (Mn), sodium (Na), and zinc (Zn) of Allium tuncelianum samples digested by microwave and ashing ovens were determined by flame atomic absorption spectrophotometry and the results were compared statistically. The Ca, Cu, K, Mg, and Zn concentrations using the microwave method were found to be higher than the ashing procedure. However, the Fe, Mn, and Na concentrations using the ashing method were found to be higher than those using the the microwave protocol.Öğe Effects of Maternal Nicotine Exposure on the Caspase-3 Expression in the Kidney Tubule Cells(Wiley-Blackwell, 2016) Yildiz, Azibe; Vardi, Nigar; Karaaslan, Merve Goksin; Yigitcan, Birgul[Abstract Not Available]Öğe Protective Effect of Infliximab on Ischemia/Reperfusion-Induced Damage in Rat Kidney(Taylor & Francis Ltd, 2012) Tasdemir, Cemal; Tasdemir, Seda; Vardi, Nigar; Ates, Burhan; Parlakpinar, Hakan; Kati, Bulent; Karaaslan, Merve GoksinObjective: To investigate the protective effect of infliximab on ischemia-reperfusion (I/R) injury of the rat kidney. Methods: Twenty-eight male Wistar albino rats were divided into four groups: sham-operated, I/R, I/R with infliximab administered before ischemia [I/R + infliximab (bi)], and I/R with infliximab administered before reperfusion [I/R + infliximab (br)]. After a right nephrectomy to produce damage, the left renal vessels were occluded for 60 min, followed by 24-h reperfusion in rats. Changes in the rat kidney were observed by measuring the tissue levels of malondialdehyde (MDA), myeloperoxidase (MPO), glutathione (GSH), and superoxide dismutase (SOD) and by evaluating hematoxylin-eosin (H&E)-stained and periodic acid-Schiff (PAS) sections. Results: The MDA and MPO levels in the I/R group were significantly higher than in the other groups (p < 0.05), and the SOD and GSH levels in the I/R + infliximab (bi) and I/R + infliximab (br) groups were significantly higher than in the I/R group (p < 0.05). However, histological examination revealed that the I/R + infliximab (bi) group and the I/R + infliximab (br) group had significantly fewer tubular changes and interstitial inflammatory cell infiltration than the I/R group. Conclusion: These results show that infliximab may protect against I/R injury in the rat I/R model.Öğe The protective effect of N-acetylcysteine amide against paraquat-induced neurotoxicity(Tubitak Scientific & Technological Research Council Turkey, 2019) Ates, Burhan; Vardi, Nigar; Parlakpinar, Hakan; Karaaslan, Merve Goksin; Yilmaz, Ismet; Ercal, NuranN-acetylcysteine amide (NACA) is a new antioxidant molecule with powerful radical scavenging properties. The aim of this study was to investigate neuroprotective effects of NACA against paraquat (PQ) toxicity in the midbrains of rats by using motor coordination tests and biochemical and histological analysis. Thirty adult Wistar albino rats were divided into three groups: Group 1: control (n = 10), Group 2: PQ (10 mg/kg) (n = 10), and Group 3: PQ (10 mg/kg) + NACA (100 mg/kg) (n = 10). NACA was administrated intraperitoneally 30 min before PQ injection. Performance was measured for a period of 28 days. The rotarod and accelerod tests were performed prior to and after the experimental period. After the experimental period, rats were sacrificed and midbrain tissues were removed. According to biochemical data, malondialdehyde levels exhibited a significant increase (P < 0.05) when the PQ group was compared to the control group, whereas the NACA-treated group showed a significant decline (P < 0.05). The total glutathione levels (P < 0.01) and the glutathione peroxidase and butyrylcholinesterase activities (P < 0.05) in the NACA treatment group were significantly raised compared with the PQ group. The main finding in the rotarod and accelerod tests was that the PQ + NACA group had improved motor coordination functions, whereas the PQ group had lost motor coordination (P < 0.05). Our histological data were also outstanding and were consistent with biochemical and motor coordination results in terms of the protective role of NACA against PQ-induced neurotoxicity.Öğe Synthesis and Radical Scavenging Properties of Selenophenyl Benzamide Anologs(Gazi Univ, 2016) Doganay, Kadir; Karaaslan, Merve Goksin; Ates, Burhan; Altundas, AliyeFree radicals that result from different kinds of oxidative stress have been concerned in a variety of human disorders, from cardiac ischemia to those affecting the central nervous system. So, there is an increasing interest in the development of antioxidant molecules that can protect cells against free radical damages. Sets of tetrasubstituted selenophene amides 4a-e were synthesized by reaction of 2-amino-5,6,7,8-tetrahydro-4H-cyclohepta[b] selenophen-3-carbonitrile (2) with benzoyl chloride derivatives and the structures of the amide derivatives were characterized by MS, H-1-NMR, C-13-NMR and IR spectra. The synthesized compounds (4a-e) were evaluated in terms of in vitro. The antioxidant properties were determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging properties and IC50 values of the compounds were in the range from 3.794 to 5.644 mg/mL. Compounds 4c and 4e showed predominant radical scavenging activity among the synthesized analogues.
