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  • Küçük Resim
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    Comparison of PTEN expression in Hashimoto thyroiditis, follicular adenoma, papillary and follicular carcinomas
    (2019) Alan, Saadet; Karadag Soylu, Nese
    Abstract: Aim: Thyroid malignancies account for approximately 3% of all human cancers. A loss or reduction in the expression of a tumor suppressor gene, PTEN has been observed in approximately 40% of thyroid tumors. This change in PTEN expression has been shown to be due to PTEN mutation or deletion. The aim of this study was to compare the PTEN expression among Hashimoto thyroiditis, follicular adenomas and malignant tumors originating from thyroid follicle epithelial cells. Material and Methods: 101 cases of thyroid carcinoma were studied and classified into differentiated types including 15 cases of follicular thyroid carcinomas (FTC), 28 cases of papillary thyroid carcinomas (PTC), 29 cases of follicular adenomas (FA) and 29 cases of Hashimoto thyroiditis (HT). PTEN expression in all the cases were analyzed immunohistochemically. Results: The cytoplasmic and nuclear staining intensity in Hashimoto thyroiditis showed minimal loss of PTEN expression. The nuclear and cytoplasmic staining intensity and percentage in Hashimoto thyroiditis and follicular adenomas were similar. In most of the papillary carcinoma samples, PTEN expression was lost as deduced from cytoplasmic and nuclear staining intensity. While the loss of nuclear PTEN expression was the highest in follicular carcinoma, the cytoplasmic loss was minimal. Conclusions: Loss of PTEN expression is more pronounced in papillary and follicular carcinomas than benign lesions. PTEN has been shown to play an oncogenic role in papillary and follicular carcinoma. PTEN expression loss can be used as a new biomarker in PTC and FTC cases.
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    Gastrointestinal amyloidosis occurring in three different patterns: Case serie
    (2019) Cagin, Yasir Furkan; Caliskan, Ali Riza; Bilgic, Yilmaz; Yildirim, Oguzhan; Erdogan, Mehmet Ali; Seckin, Yuksel; Alan, Saadet; Karadag Soylu, Nese
    Systemic amyloidosis is a rare disease characterized by extracellular accumulation of amyloid protein in one or more organs. In patients with systemic amyloidosis, the most frequently affected organs are kidney and heart, followed by the nervous system, soft tissues, and lungs. Small bowel and liver involvement are also frequent in systemic amyloidosis. Gastrointestinal (GI) findings are common, and the degree of organ involvement determines the symptoms. Patients usually have nonspecific findings such as abdominal pain, nausea, diarrhea, and dysphagia, which may delay the appropriate diagnosis. Liver involvement occurs in the majority of patients, but the symptoms typically do not happen unless a marked hepatic amyloid deposition occurs. Diagnosis is by tissue biopsy. Treatment and prognosis depend on the underlying disease. GI system involvement is a sign of poor prognosis. In this case series, five patients who were diagnosed with gastrointestinal system amyloidosis in our clinic are presented.
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    The Role of Aurora Kinase A in HBV-Associated Hepatocellular Carcinomas: A Molecular and Immunohistochemical Study
    (Mdpi, 2026) Huz, Mustafa; Karadag Soylu, Nese; Koc, Ahmet; Kucukakcali, Zeynep; Danis, Nefsun; Ozhan, Onural
    Objectives: Although Aurora kinase A (AURKA) expression has been investigated in many cancer types, studies focusing on its role in hepatitis B virus-associated hepatocellular carcinoma (HBV-HCC) are limited. In this study, we examined the activity of AURKA and its substrates (PLK1, P53, and BRCA1) in HBV-HCC and cryptogenic hepatocellular carcinoma (Cr-HCC) cases. Methods: The study groups consisted of HBV-HCC, Cr-HCC, and healthy liver tissue cases. AURKA copy number variation (CNV) was analyzed using molecular methods. AURKA expression was evaluated by molecular and immunohistochemical (IHC) methods. AURKA substrates P53(Ser315), PLK1(Thr210), and BRCA1 were also analyzed by IHC. Results: There was no increase in AURKA gene copy number among the groups (2-triangle triangle Ct < 2). AURKA level was significantly increased in both test groups (p < 0.001). At the protein level, AURKA was significantly higher in both cancer groups compared to the control group (p < 0.001). Phospho-P53(Ser315) levels were significantly higher in both HBV-HCC and Cr-HCC groups compared to the control group (p = 0.002 and p < 0.001, respectively). Cr-HCC cases also showed significantly higher levels compared to HBV-HCC (p = 0.025). For phospho-PLK1(Thr210), Cr-HCC cases showed statistically higher expression compared to both the control group and HBV-HCC cases (p = 0.001).