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Öğe Investigation of prevalence, serotyping, and antibiotic resistance of Listeria monocytogenes in samples of meat, milk, and cheese from eastern Turkey(M H Schaper Gmbh Co Kg, 2022) Cebeci, Tugba; Karagulle, Burcu; Otlu, BarisThe aim of the present study was to investigate Listeria monocytogenes contamination in ground meat purchased from local butchers and raw cow milk and tulum cheese samples sold in public markets to identify serotypes of the obtained isolates and examine their antibiotic-resistance profiles. We microbiologically tested 300 samples of different origins for the presence of L. monocytogenes and confirmed the isolates found using matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) and polymerase chain reaction, (PCR) after which they were serotyped and tested for antibiotic resistance against 8 antimicrobial agents using the disk diffusion method. L. monocytogenes was identified in 18 (6%) all of the collected samples. The serotypes of the 18 L.monocytogenes isolated in our study were 1/2b (3b) in 9 (50%), 1/2c (3c) in 4 (22.2%), 4b (4e, 4e) in 3 (16.7%), and 1/2a (3a) in 2 (11.1%) of the isolates. All isolates were found to be sensitive to ampicillin, erythromycin, gentamicin, chloramphenicol, penicillin, tetracycline, vancomycin, and sulfamethoxazole/trimethoprim. Although the isolates were sensitive to all commonly used antibiotics and given that L. monocytogenes serotypes were isolated in the examined samples, this pathogen should be closely monitored and its prevalence should be controlled using precautionary measures.Öğe The Management Practices and Microbiological Quality of a Dairy Farm with Low Bulk Tank Milk Somatic Cell Count(Univ Agriculture, Fac Veterinary Science, 2017) Risvanli, Ali; Seker, Ibrahim; Saat, Nevzat; Karagulle, Burcu; Koseman, Abdurrahman; Kaygusuzoglu, ErdalIn this study, we present the management practices and microbiological quality of a dairy farm with low bulk tank milk somatic cell count (BTMSCC). In the mentioned farm, BTMSCC was generally <150,000 cells/mL. BTMSCC per day and bulk tank milk (BTM) microbiological analysis per week were carried out during one year. Weekly animal and udder health controls and management evaluations were performed; the somatic cell counts (SCCs) of the milk samples collected in only 21 weeks of the year were over 150,000 cells/mL but this value was never over 320,000 cells/mL in the herd. When the management practices of the weeks with high BTMSCC were evaluated, negative conditions including changes of paddocks of the animals and estrus synchronization were detected. In the samples taken from the milk collection tank for a total of 22 weeks, microbiological isolation occurred; the most common bacterium was Escherichia coli. Udder hygiene, barn hygiene, the cleanliness of the beddings, the care of the employees toward their work and personal hygiene, and disinfection of the milking machines and their maintenance were all very good for the whole year. During the weekly routine controls, clinical mastitis and teat stenosis were detected twice and once, respectively; however, in those 3 weeks, BTMSCC increased in only the week that the teat stenosis was observed. We observed that, even in farms with intense precautions, BTMSCC may increase and microbiological growth may occur in BTM. To ensure that these situations do not become permanent, these precautions should be applied continuously. (C) 2017 PVJ. All rights reserved