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Öğe Development of a Liquid Chromatography-Tandem Mass Spectrometry Method for Quantifying Teicoplanin and Its Application in Critically Ill Patients(Doc Design Informatics Co Ltd, 2025) Memis, Hasan; Cakir, Ahmet; Gun, Zeynep Ulku; Saracoglu, Hatice; Karakukcu, Cigdem; Esmaoglu, Aliye; Dogan, ZaferObjective: Teicoplanin, a glycopeptide antibiotic, is used to treat infections caused by Grampositive pathogens. Trough-level monitoring of teicoplanin is recommended in specific patient populations, including critically ill patients. This study aimed to develop and validate a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to quantify teicoplanin in human plasma and adapt the method to a critically ill patient sample. Materials and Methods: Teicoplanin trough levels were measured using a newly validated LC-MS/MS method. Analysis was conducted using a C18 column with an inner diameter of 2.7 mu m (50.0 x 3.0 mm), and vancomycin hydrochloride was used as the internal standard. The method's run time per sample was 5.5 minutes. Non-parametric tests were used for statistical analysis. Univariate and multivariate logistic regression were performed to identify teicoplanin target attainment factors. A p-value of <0.05 was considered statistically significant. Results: The method demonstrated linearity between 1.56-100 mg/L teicoplanin concentration and had a lower limit of detection and quantification of 0.33 mg/L and 1.00 mg/L, respectively. Precision, accuracy, recovery rate, and carry-over effects were all within acceptable limits, according to the U.S. Food and Drug Administration (FDA) guidance. Twenty patients were included in the study. The target teicoplanin trough level (>= 10 mg/L) attainment rate was 50%. The patient's laboratory values did not significantly change after teicoplanin treatment (p>0.05), except for erythrocyte count, haemoglobin, and haematocrit values, which decreased significantly (p<0.05). Multivariate analysis revealed no significant factors affecting target attainment (p>0.05). Conclusion: The LC-MS/MS assay validated in this study is high-throughput, robust, and quick enough to be implemented in clinical therapeutic drug monitoring (TDM) laboratories. More large-scale studies are needed to understand better the relationship between teicoplanin trough levels and patient-related factors.Öğe Phenotype frequencies of blood group systems and alloantibodies to red blood cells in blood transfusion recipients in Kayseri (Turkey)(2017) Altuner Torun, Yasemin; Kaynar, Leylagül; Karakukcu, Cigdem; Yay, Mehmet; Ergul, Ayse Betul; Turanoglu, Cem; Cetin, Mustafa; Eser, BülentAim: In this study, we aimed to asses the antigen and phenotype frequencies of various blood groups among recipients of erythrocyte suspensions in Kayseri. Material and Methods: The study was conducted as retrospective, cross sectional and multicenter study. In all, 48750 blood recipients old typed in terms of the ABO, Rh, MNS, Duffy, Lewis, P, Kell, Lutheran, and Kidd systems were subjected to erythrocyte phenotyping using a gel centrifugation method within the age group of 18-60 years. Results: Of the ABO blood group, A was most frequent (44%) followed by O, B, and AB (30.3%, 16.2%, and 6.5%, respectively). The frequencies of Rh antigens were 88% D-positive and 12% D-negative. Alloantibodies were detected by screening in 196 (0.4%) of 48,750 patients, and decreased in the order anti-E (62%), -C (43%), -D (42%), -C (11%), -c (11%), -e (8%), -M (7%), -Fy a (5%), -Jk a (5%), -Kp a (4%), -Le a (3%), -Jk b (2%) ,-S (2%), -Le b (1%), and -P (1%). The most frequently detected alloantibodies were anti-E (31.6%) and -K (21.9%). Conclusion: Knowledge of the phenotypic frequencies of red blood cell antigens allows the creation of banks of appropriate antigennegative blood, thus preventing transfusion reactions in patients requiring multiple transfusions or who express alloantibodies.Öğe TEICOPLANIN TROUGH LEVEL MONITORING OF ADULT CRITICALLY ILL PATIENTS: A PROSPECTIVE LONGITUDINAL STUDY(Springer, 2025) Memis, Hasan; Cakir, Ahmet; Gun, Zeynep U.; Saracoglu, Hatice; Karakukcu, Cigdem; Esmaoglu, Aliye; Dogan, Zafer[No abstract available]
