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  • Küçük Resim
    Öğe
    Evaluation of the effects of various agents on aggregatibacter actinomycetemcomitans, candida albicans, and streptococcus mutans growth
    (2019) Taskan, Mehmet Murat; Karaman, İsa; Balci Yuce, Hatice; Isiker Kara, Gozde
    Aim: Oral environment hosts many microorganisms and even symbiotic microorganisms can cause diseases under appropriate conditions and antimicrobial therapy might be required. The aim of the present study was to evaluate the effect of various antioxidants on Aggregatibacter actinomycetemcomitans, Candida albicans, and Streptococcus mutans as pathogenic microorganisms of the oral environment.Material and Methods: In present research, the antibacterial effects of various antioxidants on oral bacteria, Aggregatibacter actinomycetemcomitans (A.actinomycetemcomitans, ATCC 33384), Streptococcus mutans (S.mutans, ATCC 35668), and fungus Candida albicans (C.albicans, ATCC 90819) were tested. The tested antioxidants were curcumin, gallic acid, and vanillic acid. The concentrations of the antioxidants were adjusted to 5% and appropriate solvents were used for the agents. The solvents were distilled water for gallic acid, ethanol for vanillic acid and dimethyl sulphoxide (DMSO) for curcumin. Antibiotics ciprofloxacin, penicillin, tetracycline, and chlorhexidine were used as positive control agents. The antibacterial effect of the antioxidants were tested with disc-diffusion method.Results: Positive control agents provided significant antibacterial efficacy compared to the test agents and negative controls. The most significant effect was observed on ciprofloxacin against all tested microorganisms. On the other hand, penicillin provided similar effects with the chlorhexidine and tetracycline. All antioxidants tested in the present study was found ineffective against all tested microorganisms. Topical use of antioxidants did not provide efficacy. Conclusion: Antioxidants have significant effects on preventing oxidative stress and decreasing inflammation-driven pathologies. However, the tested antioxidants did not provide an antibacterial effect against A.actinomycetemcomitans, S.mutans, and C.albicans.
  • Küçük Resim
    Öğe
    Growth behavior of eikenella corrodens and streptococcus gordonii in response to a short chain fatty acid metabolite-acetic acid
    (2017) Balcı Yüce, Hatice; Tulu, Feyza; Inis, Sule; Karaman, İsa
    Abstract:Aim: Periodontal diseases are chronic, inflammatory and infectious diseases. Therefore, periodontal treatment aims to eliminate periodontopathogenic bacteria causing periodontal diseases. The aim of present study was to evaluate the effect of a bacterial end metabolite, acetic acid, on periodontopathogenic bacteria, Streptococcus gordonii and Eikenella corrodens. Material and Method: In present research, Eikenella corrodens (ATCC® 23834™) and Streptococcus gordonii (NCTC 7870) were tested. Acetic acid was used in 5% concentration dissolved in distilled water. Negative control agent was distilled water and positive control agents were 0.012% chlorhexidine, penicillin, tetracycline and ciprofloxacin. The antibacterial efficacy of acetic acid against bacteria was tested via disc-diffusion method, MIC test and minimum bactericidal concentration tests. Results: The inhibition zone of ciprofloxacin, penicillin, tetracycline, CHX and acetic acid against Eikenella corrodens and Streptococcus gordonii were 32 and 37 mm, 16 and 14 mm, 21 and 16 mm, 13 and 17 mm, and 14 and 11 mm respectively. Ciprofloxacin and penicillin inhibited bacterial growth in MIC and MBC tests against both bacteria. MIC tests of acetic acid and chlorhexidine against Eikenella corrodens revealed inhibitory effect at 7.81 μl/mL and 0.97 μl/mL concentrations, respectively. Against Streptococcus gordonii, MIC of acetic acid and chlorhexidine were 1.95 μl/mL and 3.90 μl/mL, respectively. Conclusion: Acetic acid is a bacterial end product and has a daily consumption as vinegar. Due to the antibacterial efficacy against periodontopathogenic bacteria, it can be useful in adjunction to periodontal treatment. Further studies to evaluate clinical use of acetic acid as mouthwash, dentifrice, gel and/or irrigation agent are necessary.