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    Comparison of the selenium level with GSH-Px activity in the liver of mice treated with 7,12 DMBA
    (Wiley, 2002) Batcioglu, K; Ozturk, C; Karagozler, A; Karatas, F
    In this study, the relationship between selenium (Se) and glutathione peroxidase (GSHPx) levels was investigated in 7,12-dimethylbenz(a)anthracene (7,12-DMBA)-treated mouse liver. The potential mammary carcinogen 7,12-DMBA, was injected intraperitoneally (20 mg kg(-1) day(-1)) into 10-12 month old female mice. After 21 days of application the mice were sacrificed and GSHPx and Se levels of Liver homogenates were measured. Se and GSHPx levels in 7,12-DMBA-treated mice were significantly lower than those of controls (p<0.05). The control group exhibited 0.9 +/- 0.066 U mg(-1) protein and 0.86 +/- 0.058 p.p.m. levels of GSHPx and Se respectively. The 7,12-DMBA-treated group had significantly (p<0.05) decreased GSHPx and Se levels (0.42+/-0.062 U mg(-1) protein and 0.69+/-0.034 p.p.m. respectively). The results show a direct relationship between Se and GSHPx activity and a negative correlation between antioxidant capacity and existence of a carcinogen in metabolism. Copyright (C) 2001 John Wiley Sons, Ltd.
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    Determination of free malondialdehyde in human serum by high-performance liquid chromatography
    (Academic Press Inc Elsevier Science, 2002) Karatas, F; Karatepe, M; Baysar, A
    Lipid peroxidation involves the oxidative deterioration of polyunsaturated fatty acids in biomembranes and generates a variety of aldehydic products including malondialdehyde (MDA). To demonstrate the occurrence of lipid peroxidation in biological systems, the production of MDA has been shown to be a relevant indicator. Therefore, we describe a new method for measurement of free malondialdehyde in human serum. A simple, rapid but sensitive method for determination of MDA in human serum was applied to goiter patients and control groups. Patients with goiter had high levels of MDA compared to control groups. Our method is fast and practical for clinical measurements, The detection limit was found to be 1.2 x 10(-8) mol L-1. (C) 2002 Elsevier Science (USA). All rights reserved.
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    The vitamin and selenium contents of apricot fruit of different varieties cultivated in different geographical regions
    (Elsevier Sci Ltd, 2003) Munzuroglu, O; Karatas, F; Geckil, H
    High-pressure liquid chromatographic and fluorometric methods were utilized to determine the vitamin (A, C, E and beta-carotene) and selenium levels in apricot fruit. The vitamins (A, C, and E), beta-carotene and selenium levels of both fresh and sulphurized (sulphur-dried) apricot fruit from several common cultivars and wild type, grown in different regions, were analysed. The effects of post-harvest processing, regional difference and cultivar type on the levels of these nutrients were investigated. Cultivated varieties possessed significantly higher vitamin C (P < 0.01), vitamin A and E (P < 0.05) than the wild type. The vitamin C levels of fresh and sulphur-dried apricot from cultivated forms, for example, were 1.8-2.7- and 2.9-4.6-fold, respectively, higher than those of wild type ones. Among cultivated varieties, H cultivar was determined to have significantly highest vitamin A, E and beta-carotene contents (P<0.05). In general, the vitamin, beta-carotene and selenium levels were determined to be significantly different, both between varieties and between different regions within the same variety. Cultivars in region ES, a high altitude region, for example, had significantly (P < 0.05) higher vitamin C content than the same cultivars grown in other regions. The selenium content, however, was significantly (P<0.01) higher only in fresh fruit from variety HB in region ES than the other two regions. (C) 2003 Elsevier Ltd. All rights reserved.