Yazar "Kaya, Ilhan" seçeneğine göre listele

Listeleniyor 1 - 2 / 2
  • Küçük Resim Yok
    Öğe
    Molecular Cloning and Sequence Analysis of the its Region of Nuclear Ribosomal DNA for Species Identification in Dodders (Cuscuta; Convolvulaceae)
    (Friends Science Publ, 2017) Keskin, Fatma; Kaya, Ilhan; Usta, Mustafa; Demir, Ibrahim; Sipahioglu, Hikmet Murat; Nemli, Yildiz
    Dodder (Cuscuta sp.) is an obligate parasitic plant that is very difficult to control. In plants the internal transcribed spacer (ITS) region of the 18S-5.8S-26S nuclear ribosomal DNA (nrDNA) has been considered one of the most important sequences for phylogenetic analysis. Here we report the analysis of nrDNA's ITS sequences as an efficient tool to study the phylogeny of dodders collected from various provinces of Eastern Anatolia (Turkey). Genomic DNA of six dodder samples belonging to 4 distinct species was extracted from body tissue samples. The sequences of 18S rRNA, ITS-1, 5.8S rRNA, ITS-2 and 26S rRNA regions of 4 Cuscuta species were determined by molecular cloning and sequencing. The identity of cloned fragments was compared to determine sequence identity using NCBI database. Bootstrap analysis of nrDNA of C. approximate, C. lupuliformis, C. campestris and C. babylonica indicated high sequence identity with similar sequences belonging to different geographical origins of the world retrieved from NCBI database. Our results clearly showed that the most stable secondary structure derived from the sequences obtained by universal ITS4 and ITS5 primers is very efficient tool for identification of Cuscuta species when used in combination with phylogenetic analysis. (C) 2017 Friends Science Publishers
  • Küçük Resim Yok
    Öğe
    Pokeweed (Phytolacca americana L.) antiviral protein inhibits Zucchini yellow mosaic virus infection in a dose-dependent manner in squash plants
    (Tubitak Scientific & Technological Research Council Turkey, 2017) Sipahioglu, Hikmet Murat; Kaya, Ilhan; Usta, Mustafa; Unal, Murat; Ozcan, Dilek; Ozer, Meryem; Guller, Abdullah
    Pokeweed antiviral protein (PAP) of Phytolacca americana L. (pokeweed) is a single-chain ribosome-inactivating protein (RIP) characterized by its ability to depurinate plant ribosomes. Here, we isolated, cloned, and expressed the ribosome inactivating protein (RIP) gene, designated as pokeweed antiviral protein type 1 (PAP I), from the summer leaves of pokeweed collected from the Black Sea region (Turkey). Our findings presented here provide direct evidence that exogenous application of PAP I causes concentration-dependent inhibition of Zucchini yellow mosaic virus (ZYMV) infection on squash plants. Squash plants were exposed to PAP I protein with and without DMSO for four consecutive days. Regular spraying of approximately 30 kDa recombinant PAP I at 2 mu g mL(-1) concentration prevented treated plants from mechanical virus infection. PAP I showed antiviral activity in 9 plants out of 15 inoculated plants. Remarkably, simultaneous application of PAP, DMSO, and ZYMV did not prevent virus infection, suggesting that PAP did not have any effect on viral RNA. In the absence of ZYMV the purified peptide was not cytotoxic for squash plants, although a reduction of plant size, possibly caused by host ribosome depurination, was observed.