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  • Küçük Resim
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    Antioxidant effects of ellagic acid on testicular tissue of rats exposed to tobacco smoke
    (2017) Kaya, Nalan; Ozan, Gonca; Dabak, Ozlem; Gur, Seyfettin; Ozan, Ibrahim Enver
    Aim: Tobacco smoke negatively affects the male reproductive system. Ellagic acid (EA) has protective effects against oxidative damage. The aim of the study was to examine the protective effects of EA on testis of rats exposed to tobacco smoke.Materials and Methods: Twenty-four male Spraque-Dawley rats were divided into 4 groups (n=6): Control, tobacco smoke (TS), tobacco smoke+corn oil (TS+C) and tobacco smoke+ EA (TS+EA). TS, TS+C and TS+EA groups were exposed to tobacco smoke 1 hour twice a day and EA was applied 12 mg/kg every other day. Testis tissues were removed. eNOS immunohistochemical stain and TUNEL methods were applied. Biochemical analyzes and sperm analyzes were performed.Results: Degeneration in germinative epithelium, cell debris in the seminiferous tubule lumen, seperation in basement membrane, atrophic tubules, vascular congestion and edema in interstitial area were observed in TS and TS+C groups. Increased apoptotic cells and eNOS immunreactivity were observed in TS and TS+C groups. EA administration caused a decrease in histological alterations, eNOS immunreactivity and apoptotic cells. Increased MDA levels, decreased CAT and GSH-Px activities were observed in TS and TS+C groups. MDA levels decreased, CAT and GSH-Px activities increased in TS+EA group. A significant increase in the amount of abnormal sperm was detected in TS and TS+C groups. The reduction in the amount of abnormal sperm was detected in TS+EA group.Conclusions: Exposure to TS led to marked alterations on testes tissue and treatment with EA might prevent these toxic effects.Keywords: Tobacco Smoke; Testis; Ellagic Acid; Rat; TUNEL.
  • Küçük Resim
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    Antioxidant effects of ellagic acid on testicular tissue of rats exposed to tobacco smoke metabolite-acetic acid
    (2017) Kaya, Nalan; Ozan, Gonca; Dabak, Dürrin Özlem; Gür, Seyfettin; Ozan, İbrahim Enver
    Abstract:Aim: Tobacco smoke negatively affects the male reproductive system. Ellagic acid (EA) has protective effects against oxidative damage. The aim of the study was to examine the protective effects of EA on testis of rats exposed to tobacco smoke. Material and Methods: Twenty-four male Spraque-Dawley rats were divided into 4 groups (n=6): Control, tobacco smoke (TS), tobacco smoke+corn oil (TS+C) and tobacco smoke+ EA (TS+EA). TS, TS+C and TS+EA groups were exposed to tobacco smoke 1 hour twice a day and EA was applied 12 mg/kg every other day. Testis tissues were removed. eNOS immunohistochemical stain and TUNEL methods were applied. Biochemical analyzes and sperm analyzes were performed. Results: Degeneration in germinative epithelium, cell debris in the seminiferous tubule lumen, seperation in basement membrane, atrophic tubules, vascular congestion and edema in interstitial area were observed in TS and TS+C groups. Increased apoptotic cells and eNOS immunreactivity were observed in TS and TS+C groups. EA administration caused a decrease in histological alterations, eNOS immunreactivity and apoptotic cells. Increased MDA levels, decreased CAT and GSH-Px activities were observed in TS and TS+C groups. MDA levels decreased, CAT and GSH-Px activities increased in TS+EA group. A significant increase in the amount of abnormal sperm was detected in TS and TS+C groups. The reduction in the amount of abnormal sperm was detected in TS+EA group. Conclusions: Exposure to TS led to marked alterations on testes tissue and treatment with EA might prevent these toxic effects.
  • Küçük Resim Yok
    Öğe
    TRPM2 mediates distruption of autophagy machinery and correlates with the grade level in prostate cancer
    (Springer, 2019) Tektemur, Ahmet; Ozaydin, Seda; Onalan, Ebru Etem; Kaya, Nalan; Kuloglu, Tuncay; Ozercan, Ibrahim Hanifi; Tekin, Suat
    PurposeTransient receptor potential melastatin 2 (TRPM2), a calcium-permeable ion channel, is shown as a prognostic marker candidate in prostate cancer (PCa) and an important regulator of autophagy. We aimed to determine the changes in TRPM2 and autophagic-apoptotic gene expression levels in human prostate adenocarcinomas, and to investigate the affect of TRPM2 on autophagic pathways in PC-3 cell line.MethodsHuman prostate tissues were classified considering the grade levels and were divided into the control, BPH, and grade 1-5 groups. mRNA expression levels of genes were determined by qPCR. In addition, TRPM2 was evaluated immunohistochemically for each group. In PC-3 cell line, TRPM2 was silenced through siRNA transfection, and autophagy induction was analyzed by acridine orange (AO) staining.ResultsThe qPCR and immunoreactivity results showed that the increased TRPM2 expression levels in human PCa samples were paralleled with higher grade levels. The autophagic-apoptotic gene expressions showed high variability in different grade levels. Also, silencing TRPM2 in PC-3 cells altered autophagic gene expressions and caused autophagy induction according to the AO staining results.ConclusionWe showed that the autophagy-TRPM2 association may take place in the molecular basis of PCa and accordingly this connection may be targeted as a new therapeutic approach in PCa.
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    Tütün dumanına maruz kalan ratlarda hesperetinin testis dokusundaki antioksidan etkisinin incelenmesi
    (İnönü Üniversitesi Sağlık Bilimleri Dergisi, 2017) Yılmaz, Osman Fatih; Kaya, Nalan; Ozan, Gonca; Kaya, Şeyma Özer; Dabak, Dürrin Özlem; Ozan, İbrahim Enver
    Amaç: Tütün dumanında bulunan ve kanla taşınan toksik maddeler oksidan antioksidan sistem arasındaki dengeyi dokular aleyhine değiştirmektedir. Antioksidan, antimikrobiyal ve antiinflammatuvar özelliklerinin olduğu bilinen hesperetin; limon, portakal ve greyfurt gibi turunçgillerde bulunur. Çalışmada tütün dumanı maruziyetinin testis dokusunda oluşturacağı hasara karşı hesperetinin koruyucu etkilerinin incelenmesi amaçlanmıştır. Gereç ve Yöntem: Çalışmada 24 adet erişkin Spraque-Dawley erkek rat kullanıldı. Ratlar 4 gruba ayrıldı (n:6):kontrol (K), tütün dumanı (TD), tütün dumanı+mısırözü yağı (TD+M) ve tütün dumanı+hesperetin (TD+HES). TD, TD+M ve TD+HES gruplarındaki ratlar, günde iki defa birer saat tütün dumanına maruz bırakıldı. TD+HES grubundaki ratlara tütün dumanına ilaveten oral gavajla, mısırözü yağında çözülmüş hesperetin gün aşırı 50 mg/kg dozunda uygulandı. TD+M yağı grubundaki ratlara ise tütün dumanına ilaveten hesperetini çözmede kullanılan miktarda mısırözü yağı gün aşırı oral gavajla verildi. 12 haftalık deney süresi sonunda tüm gruplardaki ratlar dekapite edildi. Testis dokuları çıkarıldı. Histokimyasal ve eNOS boyamaları ile TUNEL yöntemi uygulandı. Biyokimyasal analizler ve sperm analizleri yapıldı. Bulgular: TD ve TD+M gruplarına ait kesitlerde seminifer tübül germinal epitelinde dejenerasyon, atrofik tübüller, vasküler konjesyon, seminifer tübül bazal membranlarında ayrılmalar, interstisyel alanda ödem ve bazı tübüllerde lümene dökülmüş immatür hücreler tespit edildi. Ayrıca bu gruplarda apoptotik hücre sayısında ve eNOS immünreaktivitesinde artış gözlendi. Hesperetin uygulamasının histolojik değişimlerde, eNOS immünreaktivitesinde ve apoptotik hücre sayısında azalmaya neden olduğu belirlendi. Malondialdehit(MDA) düzeyleri TD ve TD+M gruplarında arttığı, hesperetin uygulamasının ise MDA düzeyini azalttığı tespit edildi. Katalaz (CAT) ve glutatyon peroksidaz (GSH-Px) enzim aktivitelerinin, TD ve TD+M gruplarında azaldığı, TD+HES grubunda ise GSH-Px aktivitesinin arttığı tespit edildi. CAT aktivitesi açısından TD, TD+M ve TD+HES grupları arasında fark bulunamadı. TD ve TD+M gruplarında anormal sperm sayısında artış, TD+HES grubunda ise azalma tespit edildi. Sonuç: Bulgular tütün dumanının testis dokusunda hasara neden olduğu ve hesperetin uygulamasının ise bu toksik etkilere karşı koruma sağladığını göstermektedir. Anahtar Kelimeler: Tütün Dumanı, Hesperetin, Histoloji, Biyokimya, Testis.