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    Cyclospora Oocysts and Entamoeba histolytica Adhesin Antigen Positivity in a Patient with Diarrhea: Case Report
    (Ortadogu Ad Pres & Publ Co, 2012) Oguzturk, Hakan; Kaya, Ozlem Aycan; Turtay, M. Gokhan; Atambay, Metin
    Cyclospora cayetanesis is a pathogenic protozoan characterized with prolonged diarrhea in immunocompetent and immunocompromised patients. Despite its ubiqutous distribution, reports on this parasite from Turkey are scarce. Our case is a 28 years old patient, a nurse, who presented to the emergency department in June 2009 with diarrhea, nausea and vomiting during the last week. The stool specimen examination by Kinyoun's acid-fast stain revealed Cyclospora oocysts. Adhesin antigen of Entamoeba histolytica was also positive. The patient was treated with a combination of trimethoprim/sulfamethoxazole and omidazol and she fully recovered. The literature was reviewed and our case proved to be the first report on the coexistance of Cyclospora oocysts and Entamoeba histolytica adhesin antigen in a diarrheic patient. This case underlines the importance of screening for parasites in patients with diarrhea.
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    Detection of Entamoeba histolytica with different methods admitted to the emergency department in diarrheic patients
    (Academic Journals, 2011) Kaya, Ozlem Aycan; Oguzturk, Hakan; Turtay, Muhammet Gokhan; Daldal, U. Nilgun
    Antigen screening were conducted to stool samples from 60 patients admitted to our emergency department with diarrhea complaint between June 2009 and October 2009 by the methods of direct microscopic examination, trichrome staining, ELISA (Enzyme-linked immunosorbent assay), respectively. As a result of examination of total 60 samples with direct microscopic, trichrome staining and ELISA method, it was detected positive in 7(11.3%), 6(9.7%) and 8(12.9%) samples, respectively. The presence of Entamoeba histolytica has been accepted exactly in the samples in which ELISA test results were positive and necessary treatment of patients has been started immediately. Due to precise pathogen protozoan discrimination has not been performed with the direct microscopic examination, it was emphasized that unnecessary drug therapy would be prevented as a result of detection of presence of E. histolytica specific antigen by ELISA in the samples sent to the laboratory with the diagnosis of amoebiasis by concerned physician.
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    Prevalence of Demodex folliculorum and Demodex brevis in childhood malnutrition and malignancy
    (Wiley, 2013) Kaya, Sadik; Selimoglu, Mukadder Ayse; Kaya, Ozlem Aycan; Ozgen, Unsal
    Background Hair follicle mites, Demodexfolliculorum and Demodexbrevis, are known to accompany immune-deficiency states, however no study so far has investigated their presence in malnutrition. In this study we aimed to determine the prevalence of those mites in childhood malnutrition, malignancy and risk factors. Methods One hundred children with malnutrition, 31 children with malignancy and 63 children without any chronic disease and infection were included in this study. History, physical examination, anthropometric measurements and routine laboratory findings were recorded. Demodex spp. were investigated by standard superficial skin biopsies. Results Demodex was found in 25 patients (25%), 10 patients (32.3%), and one patient (1.6%) among malnutrition, malignancy, and control groups, respectively (P = 0.001). By using multilogistic regression binary method, it was found that malnutrition, malignancy and low socioeconomic level increased the risk 17.37 times (P = 0.006), 27.29 times (P = 0.002), and 2.3 times (P = 0.037), respectively. Of 22 children who were evaluated after 6 months, 13 (59.1%) were negative for Demodex. In 11 (84.6%) of those 13, nutritional status was improved. Conclusion Demodex was detected in approximately in one-quarter and one-third of children with malnutrition and malignancy, respectively. Eliminating the cause of immunosuppression, such as poor nutritional status, seems also to be an effective method for eliminating Demodex.
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    Sociodemographic characteristics of patients with Demodex brevis and Demodex folliculorum infestation and its association with rosacea and Behcet's disease.
    (Allied Acad, 2015) Durmaz, Suleyman; Yula, Erkan; Kaya, Ozlem Aycan; Gokmen, Aysegul Aksoy; Kilinc, Cetin; Atambay, Metin; Ekiz, Ozlem
    Demodex mites are ectoparasites often found in the skin thought to be associated with the development of some skin diseases. Demodex positivity may be affected by several factors. In order to determine the relationship between Demodex infestation and rosacea disease (RD), Behcet's disease (BD) and various individual parameters such as gender, personal hygiene and skin type. A total of 238 individuals (61 with RD, 46 with BD, and 131 healthy subjects) were investigated for the presence of Demodex folliculorum and D. brevis using the standardized skin surface biopsy technique and a questionnaire. The incidence of Demodex mites in RD patients was significantly higher when compared to the control group (p=0.010 and p=0.001, respectively). However, there was no difference between those with BD and the control group. There was a significant difference between ages of groups, skin types, use of moisturizer, and itching, without taking into account the presence of Demodex among the disease groups (p<0.05). On the other hand, there was no significant difference between disease groups in terms of data of gender, skin care, epilation, and face washing. Our study reveals that the positivity rates of Demodex mites in rosacea patients were significantly higher. Mean ages of groups, skin types, use of moisturizer, and the presence of itching of the ear and face may be associated with higher density of Demodex mites.