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    Ameliorative effects of thymoquinone on the caspase 3, kidney function and oxidative stress tartrazine-induced nephrotoxicity
    (Pergamon-Elsevier Science Ltd, 2024) Erdemli, Zeynep; Gul, Mehmet; Gokturk, Nurcan; Kayhan, Elif; Demircigil, Nursena; Ozsoy, Eda Nur; Bag, Harika Gozukara
    First in the literature this study aimed to investigate the effects of Tartrazine, a common industrial food dye, on kidney and whether Thymoquinone has a protective effect in tartrazine-induced nephrotoxicity. The study conducted on the rats bred at I(center dot)non & uuml; University Experimental Animals Production and Research Center. Wistar albino rats were randomly divided into 4 groups, where each group included 8 rats: control, Tartrazine, Thymoquinone, and Tartrazine + Thymoquinone groups. The experiments continued for 3 weeks and then, kidney tissues and blood samples were collected from the rats under anesthesia. Malondialdehyde (MDA), super oxidized dismutase (SOD), total oxidant status (TOS), increase in Oxidative stress index (OSI), glutathione (GSH), Glutathione peroxidase (GSH-Px), catalase (CAT), Total antioxidant status (TAS) levels decreased in the kidney tissues collected from the tartrazine group. Serum Bun and Creatinine levels increased in the tartrazine group. Tartrazine administration damaged and degenerated the glomeruli and cortical distal tubes in the histopathology of kidney tissues, also different degrees of inflammatory cell infiltration were observed in the renal cortex and medulla. Thymoquinone and tartrazine administration improved both biochemical and histopathological parameters. Tartrazine administration induced nephrotoxicity. This could be observed with the increase in oxidant capacity and the deterioration of kidney functions. Thymoquinone was observed to demonstrate strong antioxidant properties. Thymoquinone could be used primarily as a protective agent against Tartrazine-induced toxicity.
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    High-fat and carbohydrate diet caused chronic kidney damage by disrupting kidney function, caspase-3, oxidative stress and inflammation
    (Elsevier Science Inc, 2024) Erdemli, Zeynep; Gul, Mehmet; Kayhan, Elif; Gokturk, Nurcan; Bag, Harika Gozukara; Erdemli, Mehmet Erman
    The study aimed to compare the effects of a diet rich in fat, carbohydrates and protein on rat kidneys. The study was conducted on 40 Wistar albino rats bred at Ino center dot nu University Faculty of Medicine after the approval of the ethics committee. Rats were randomly divided into 4 groups: Control group, and the groups where the animals were fed with high carbohydrate, fat and protein rich feed. After the applications, the rat kidney tissues were removed by laparoscopy under anesthesia and blood samples were collected. 13 weeks long fat-rich and carbohydrate feed application had negative effects on oxidant-antioxidant balance, oxidative stress index, inflammation markers, kidney functions tests, histopathology and immunohistochemistry caspase-3 findings in rat kidney tissues, especially in the carbohydrate group when compared to the controls. Protein-rich feed, there were no significant difference in biochemical and histopathology compared to the control group. Fat and carbohydrate rich feed led to an increase in oxidative stress in rat kidney tissues. Oxidative stress led to nephrotoxicity, which in turn led to chronic kidney tissue damages. A more balanced and protein-rich diet instead of excessive sugar and fatty food intake could be suggested to prevent chronic kidney damage.
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    Prepubertal dönemden erişkin döneme kadar uygulanan farklı beslenme tiplerinin testis, spermatogenez ve sperm kalitesi üzerine etkilerinin araştırılması
    (İnönü Üniversitesi, 2024) Kayhan, Elif; Gül, Mehmet; Ozan, İbrahim Enver
    Amaç: Farklı tip beslenmenin testis, spermatogenez, sperm gelişimi üzerine etkileri incelenerek beslenme ile infertilite arasındaki ilişkinin tespit edilmesi. Materyal ve Metot: Wistar Albino cinsi 40 adet, 21 günlük erkek sıçan, dört gruba ayrıldı (n=10). K grubu; Standart, Kh grubu; %70 karbonhidrat ağırlıklı, Y grubu; %60 yağ ağırlıklı ve P grubu; %50 protein ağırlıklı yem ile 13 hafta beslendi. Deney sonunda VKİ ve sperm analizleri yapıldı. Testis kesitlerinde ışık mikroskobik olarak histolojik ve histomorfometrik inceleme, IHC yöntemle anti-Caspase-3 ve anti-Okludin immünreaktiviteleri belirlendi. Ultra ince kesitlerde TEM incelemeleri yapıldı. Serumda FSH, LH, T ve Adiponektin düzeyleri, testis doku homejenatlarında MDA, KAT, SOD, TAS ve TOS seviyeleri belirlendi. Bulgular: Kh grubunda germinal epitel yüksekliğinde azalma, Johnsen's skorunda düşme, germinatif hücrelerde dejeneratif değişiklikler, bazal membranda artmış PAS pozitiflik ve ondülasyon izlendi. Kaspaz-3 immünreaktivitesinde artış, okludin immünreaktivitesinde azalış tespit edildi. Kh grubunda TOS da artış, TAS da azalma görüldü. Sperm sayısının ve motilitesinin K grubuna kıyasla azaldığı, sperm anomalisinin arttığı tespit edildi. P grubunda histolojik hasarın azaldığı, germinal epitel yüksekliği ve Johnsen's skorunun K grubuna benzer olduğu görüldü. Kaspaz-3 immünreaktivitesinde azalış okludin immünreaktivitesinde artış izlendi. TOS düzeyinde azalma, P grubunda serum FSH, LH ve total T seviyesi tüm gruplara kıyasla artmış tespit edildi. Serum adiponektin seviyesi ise; Kh grubunda diğer gruplara kıyasla anlamlı derecede düşük, P grubunda ise öbür gruplara kıyasla anlamlı derecede yüksek tespit edildi. Sonuç: Uzun süreli Kh ve Y ağırlıklı besin diyetlerinin testiküler hasarı arttırarak, spermatogenez sürecini ve bu süreçle ilişkili hormon düzeylerini bozarak sperm rezervini ve kalitesini azaltarak infertiliteye sebep olabilir.