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    An investigation of the relationship between base deficit and CRP in asphytic infants
    (2020) Gumus, Huseyin; Kazanasmaz, Halil; Aslan, Halil; Solmaz, Abdullah
    Aim: To investigate the relationship between base deficit (BD) and C-reactive protein (CRP) findings evaluated during diagnosis of infants followed up for a diagnosis of perinatal asphyxia (PA).Material and Methods: This prospective observational study included 66 cases as 33 cases treated and followed up for a diagnosis of PA in the Neonatal Intensive Care Unit of Harran University Medical Faculty Hospital, and a control group of 33 healthy infants. Using a syringe washed with heparin, a fetal blood sample of 2ml was taken from the umbilical artery of the neonates thought to have PA, and blood gases were examined in an anaerobic environment. CRP values were obtained from spectrophotometric biochemical analysis of the 2ml blood sample using an Architect C16000 device (Abbott Diagnostics, Abbott Park, IL, USA). Results: Evaluation was made of 33 infants with a diagnosis of PA hospitalised in NICU and a control group of 33 healthy infants. All the cases were statistically similar in respect of gestational week at birth (GW), birthweight (BW), and gender. In the blood gas examinations of the cord blood, pH value was determined as mean 6.9 (range, 6.50-7.06), mean BD as 17mmol/L (range, 12-28 mmol/L), and CRP values as 0.2-57 mg/dl. The CRP values of the PA cases on days 2 and 3 were determined to be significantly higher than those of the control group (p0.001). As the BD value at the time of diagnosis increased, so there was determined to be a significant increase in CRP value, and this was confirmed by the Spearman correlation test (r1:0.423, p1:0.014) (r2:0.342, p2:0.05) (r3:0.451, p3:0.009). No statistically significant relationship was determined between the pH value in blood gas and the CRP level (p:0.18). Conclusion: The results of this study demonstrated that the CRP level was statistically significantly higher in cases that developed asphyxia than in the control group. A positive correlation was determined between BD at diagnosis and CRP level. A high CRP level during treatment and follow-up can be considered for use as a supportive finding showing the severity of hypoxia.
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    Specificity and sensitivity of biotinidase activity measured from dried blood spot by colorimetric method
    (2019) Kazanasmaz, Halil; Atas, Nurgul; Karaca, Meryem
    Aim: A variety of methods are used to determine biotinidase activity (BA), such as different substrates, biological samples, and analytical techniques. In this study, we aimed to discuss the specificity and sensitivity of the fluorometric method used in the measurement of biotinidase activity in the newborn screening program (NSP) in Turkey.Material and Methods: Medical records of 164 patients who were referred to our clinic with the diagnosis of biotinidase deficiency (BD) from primary health care institutions were evaluated retrospectively. According to this classification, those with normal BA were included in the negative group and those with partial or profound BD were included in the positive group.Results: Four patients had profound and 66 patients had partial BD, whereas 94 patients had normal BA. ROC analysis was performed to determine the specificity and sensitivity of BA from dry blood spot. Analysis showed 41% sensitivity and 39% specificity for BA≥56.91 MRU (AUC: 0.339, p 0.001). Conclusion: TIt was thought that low efficacy of fluorometric method used for the diagnosis of BD in the NSP may cause diagnostic delay. It is thought that a more effective neonatal screening program can be applied by using tandem mass spectrometer method.