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Öğe The efficacy of laser, ultrasound and self-adjustable file in removing smear layer debris from oval root canals following retreatment: A scanning electron microscopy study(Wiley, 2016) Keles, Ali; Kamalak, Aliye; Keskin, Cangul; Akcay, Merve; Uzun, IsmailThis study aims to compare the efficacy of different irrigation activation methods to remove smear layer and debris from oval-shaped root canals following retreatment. Ninety mandibular canines with oval-shaped root canals were selected. Retreatment was performed with R-Endo retreatment files. The samples were randomly divided into six groups (n=15). Groups were assigned according to agitation technique: R-Endo with classic irrigation, with self-adjustable file, with passive ultrasonic irrigation, R-Endo+Er:YAG, R-Endo+PIPS and R-Endo+Nd:YAG. Compared with the classic irrigation (R-Endo+CI) group, all other groups were more successful in reducing smear layer and debris (P<0.05). The least amount of residual smear layer and debris was detected in the Er:YAG laser group (P<0.05). Additional use of different agitation methods contributes to removal of debris and smear layer following root canal filling removal with rotary instruments; however, none of the techniques tested render root canal walls completely free of smear layer or debris.Öğe The efficacy of laser, ultrasound and self-adjustable file in removing smear layer debris from oval root canals following retreatment: A scanning electron microscopy study(Wıley, 111 rıver st, hoboken 07030-5774, nj usa, 2016) Keles, Ali; Kamalak, Aliye; Keskin, Cangul; Akcay, Merve; Uzun, IsmailThis study aims to compare the efficacy of different irrigation activation methods to remove smear layer and debris from oval-shaped root canals following retreatment. Ninety mandibular canines with oval-shaped root canals were selected. Retreatment was performed with R-Endo retreatment files. The samples were randomly divided into six groups (n=15). Groups were assigned according to agitation technique: R-Endo with classic irrigation, with self-adjustable file, with passive ultrasonic irrigation, R-Endo+Er:YAG, R-Endo+PIPS and R-Endo+Nd:YAG. Compared with the classic irrigation (R-Endo+CI) group, all other groups were more successful in reducing smear layer and debris (P<0.05). The least amount of residual smear layer and debris was detected in the Er:YAG laser group (P<0.05). Additional use of different agitation methods contributes to removal of debris and smear layer following root canal filling removal with rotary instruments; however, none of the techniques tested render root canal walls completely free of smear layer or debris.Öğe Visualization and characterization ofEnterococcus faecalisbiofilm structure in bovine dentin using 2D and 3D microscopic techniques(Springer, 2021) Keles, Ali; Keskin, Cangul; Kalkan, Melis; Yakupogullari, Yusuf; Gul, Mehmet; Aydemir, Hikmet; Sahin, FikrettinBacterial biofilms are related to various dental and periodontal infectious diseases, and the characterization of this biological structure with micro-computed tomography (micro-CT) may offer valuable information for clinical and research applications. In this study, we aimed to develop a model to visualize three-dimensionally the biofilm structure on dentin using micro-CT. Dentin blocks were prepared and incubated in tryptic soy broth withEnterococcus faecalis(ATCC 29212). The control group did not receive any staining procedure, while groups 1 and 2 were stained with 100% and 50% barium sulfate, respectively. Transmission electron microscopy (TEM) and confocal laser scanning microscopy (CLSM) were used to detect biofilm formation, barium sulfate penetration, and microbial cell density in the biofilm. Micro-computed tomography (micro-CT) (SkyScan 1172, Bruker Co., Belgium) was used to visualize biofilm formation on the dentin blocks. Biofilm thicknesses were measured from 10 different locations on the specimen surfaces, using CTAn v.1.14.4 software. Obtained data were statistically analyzed using Kruskal-Wallis and Dunn's tests. TEM photomicrographs showed that barium sulfate could penetrate the biofilm structure. CLSM analysis showed that viable and total cell densities were similar between the control and barium sulfate-treated groups (P > 0.05), indicating barium sulfate had no significant influence on cell density. In barium sulfate-treated blocks, biofilm could be discriminated from the dentin, and its thickness could be measured with micro-CT. This study showed that bacterial biofilm on dentin could be characterized by micro-CT after barium sulfate staining without causing any significant side effect on viable and total cell densities.