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    Influence of Leaf Water Content on the Prediction of Nutrient Stress in Strawberry Leaves using Chromameter
    (Friends Science Publ, 2018) Keskin, Muharrem; Sekerli, Yunus Emre; Gunduz, Kazim
    Chemical soil and plant tissue analyses require considerable amount of labor, time, and cost. Non-destructive prediction of nutrient stress from chromameters may save time and labor. Previous studies did not assess multiple nutrient stresses together with the effect of leaf water content (WC). The aim of this work was to investigate the effect of leaf WC on the prediction of leaf nutrient stress from leaf color. A commercial strawberry field with a significant amount of leaf color variability was selected in Hatay province of Turkey. Forty eight leaf samples with varying colors were collected. A hand-held chromameter was used for leaf color measurements in two color systems as L*a*b* and L*C*h degrees. Leaf WC was determined using oven drying method. Leaf nutrient contents of N, P, K, Ca, Mg, Mn, Fe, and Cu were obtained using chemical analysis. Correlation and Partial Least Square Regression (PLSR) methods were utilized for data analysis. Leaf WC ranged from 58.3 to 65.7%. High correlation existed between color parameters and N, Ca, and WC (|r|>0.66). Also, correlation was high between leaf WC and leaf N content (r=0.75), suggesting that the leaves with high WC had also high N content. Using color data, it was possible to predict leaf N content (R-2=0.66), Ca content (R-2=0.70), and WC (R-2=0.65). Using WC as a variable together with the color parameters slightly improved the model performance to predict strawberry leaf nutrient concentrations. (C) 2018 Friends Science Publishers
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    Serum soluble TWEAK levels in non-alcoholic fatty liver disease
    (2019) Asil, Mehmet; Demir, Ali; Unver, Nurcan; Polat, Hakki; Ataseven, Huseyin; Uysal, Saliha; Kayar, Yusuf; Keskin, Muharrem; Yolacan, Ramazan; Biyik, Murat; Dertli, Ramazan
    Aim: Non-alcoholic fatty liver disease (NAFLD) is the most common chronic liver disease. The exact pathogenesis of NAFLD has not been fully elucidated. Tumor necrosis factor-like weak inducer of apoptosis (TWEAK) is a member of TNF superfamily and it has been implicated in the pathogenesis of several diseases including liver inflammation and fibrosis. Current study was conducted to evaluate serum sTWEAK levels in patients with NAFLD.Material and Methods: Seventeen patients with biopsy proven non-alcoholic steatohepatitis (NASH), 22 patients with simple hepatosteatosis and 30 healthy controls were included in the study and serum sTWEAK concentrations were measured using commercial ELISA kits.Results: Mean serum sTWEAK concentration was significantly lower in the NASH group when compared to the simple hepatosteatosis group and healthy controls (199.6±101.2 pg/mL, 246.1±65.7 pg/mL and 277.6±117.6 pg/mL respectively, p=0.029). ROC analyses for sTWEAK to differentiate NASH patients from healthy controls and from simple hepatosteatosis revealed that AUC for sTWEAK was 0.712 (%95 CI, 0.543-0.880). For the specified cut off value, 171.1 pg/mL positive and negative predictive values calculated were 64.3% and 85.5% respectively. Conclusion: Serum sTWEAK concentration is decreasedin patients with NASH when compared to patients with simple hepatosteatosis and healthy controls. Keywords: Nonalcoholic steatohepatitis; tumor necrosis factor-like weak inducer of apoptosis; sTWEAK; fibroblast growth factor-inducible 14; Fn 14.
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    SEVERE HYPERBILIRUBINEMIA DURING FAMILYAL MEDITERRANEAN FEVER ATTACK IN PATIENTS WITH GILBERT'S SYNDROME
    (Istanbul Univ, Faculty Medicine, Publishing Office, 2016) Kutlu, Orkide; Keskin, Muharrem; Basturk, Abdulkadir; Ecirli, Samil; Bilgic, Yilmaz; Fisekci Oktar, Sevil
    Familial Mediterranean fever (FMF) is an autosomal recessive disorder characterized by fever and accompanying attacks of serositis. Cytokines such as IL-6, TNF-alpha, IL-1 beta are known to play an active role in FMF pathogenesis. Gilbert's syndrome, is a clinical condition that results indirect hyperbilirubinemia during febrile illness, surgery, vomiting and prolonged fasting period because of inhibition of UDP-glucuronide transferase activity. Increased bilirubin levels during FMF attack is caused suppression of UDP-glucuronide transferase activity by cytokines involved in the pathogenesis of FMF. Here, we report a patient who had serious indirect bilirubin elevation during an attack of FMF.