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Öğe Asprosin Takes a Protective Role in Mice Established Experimental Acute Kidney(Wiley, 2023) Korkmaz, Engin; Keskin, Tuba; Yildiz, Azibe; Tekin, Cigdem; Tekin, Suat; Beytur, Ali[Abstract Not Available]Öğe Central Application of Asprosin Increases Secretion of Male Reproductive Hormones: An In Vivo Study(Wiley, 2022) Keskin, Tuba; Erden, Yavuz; Tekin, Suat[Abstract Not Available]Öğe Erkek sıçanlara intraserebroventriküler asprosin uygulamasının üreme hormonları üzerine etkileri(İnönü Üniversitesi, 2021) Keskin, TubaAmaç: Asprosin beyaz yağ dokudan salgılanan ve hepatik glikoz salınımını arttıran protein yapılı bir hormondur. Asprosinin beyin ve testis dokusunda lokalize olduğu, bununla birlikte asprosin uygulamasının spermlerin ilerleyici hareketini arttırdığı bilinmektedir. Bu çalışma, merkezi asprosin infüzyonunun hipotalamus hipofiz testiküler aksında görev alan hormonlar ile sperm hücreleri üzerindeki etkilerini araştırmak amacıyla yapıldı. Materyal ve Metot: Çalışmada 40 adet Spraque Dawley ırkı erkek sıçan kullanıldı. Sıçanlar kontrol, sham, 34 ve 68 nM asprosin olmak üzere dört gruba ayrıldı (n=10). Kontrol grubuna herhangi bir uygulama yapılmazken sham ve deney gruplarındaki sıçanların lateral ventriküllerine beyin infüzyon kiti implant edildi ve 14 gün süresince infüzyon (Sham grubu için yapay beyin omurilik sıvısı, uygulama grupları için 34 ve 68 nM asprosin) gerçekleştirildi. Deney sonunda sıçanların hipotalamus, kan ve epididimis dokuları toplandı. Hipotalamus dokusunda RT-PCR ve Western Blot yöntemleriyle GnRH mRNA ve doku protein düzeyleri belirlendi. Kan örneklerinden ELISA yöntemi kullanılarak serum LH, FSH ve testosteron seviyeleri, epididimis dokusunda ise sperm hücreleri incelendi. Bulgular: Asprosin uygulanan grupların GnRH mRNA ve protein ifadeleri kontrol ve sham gruplarına kıyasla yüksekti (p<0.05). Asprosin infüzyonunun serum FSH, LH ve testosteron seviyelerini de arttırdığı belirlendi (p<0.05). Ayrıca asprosin uygulanan gruplarda sperm yoğunluğunun, hareketliliğinin ve ileri hareketinin arttığı görüldü (p<0.05). Sonuç: Bu çalışma merkezi olarak uygulanan asprosinin hipotalamus hipofiz testiküler aksta görev alan hormonlar ve epididimis dokusu üzerinde aktive edici özelliğe sahip olabildiğini ortaya koymaktadır. Elde edilen sonuçlar asprosinin infertilite tedavisinde ele alınması gereken önemli bir protein/biyobelirteç olduğunu düşündürmektedir. Anahtar Kelimeler: Asprosin, FSH, GnRH, LH, Testosteron, Sperm.Öğe Intracerebroventricular asprosin administration strongly stimulates hypothalamic-pituitary-testicular axis in rats(Elsevier Ireland Ltd, 2021) Keskin, Tuba; Erden, Yavuz; Tekin, SuatAsprosin, a protein-based secretary product of white adipose tissue, stimulates appetite hepatic glucose production. It crosses blood-brain barrier and stimulates appetite center and causes sperm chemotaxis but exact role of this endogenous agent is not completely known. This study was conducted to investigate possible effects of central asprosin infusion on the hormones involved in the hypothalamic-pituitary-testicular (HPT) axis and sperm cells. Spraque Dawley male rats were divided into four groups; control, sham, low asprosin (34) and high asprosin (68 nM) groups, (n = 10 for each group). Control group remain intact while a brain infusion kit was placed in the lateral ventricles of the rats in the sham group (artificial cerebrospinal fluid) and asprosin (34 and 68 nM) was infused for 14 days. At the end of the experiment, the hypothalamus, blood, and epididymis tissues of the rats were collected. Gonadotropin-releasing hormone (GnRH) mRNA and tissue protein levels were determined in the hypothalamus tissue by RT-PCR and Western Blot methods. Serum luteinizing hormone (LH), follicle-stimulating hormone (FSH), and testosterone levels were examined using the ELISA method from blood samples and sperm cells were examined in the epididymis tissue. GnRH mRNA and protein expressions of asprosin administered groups were higher than control and sham groups (p < 0.05). Asprosin infusion was also found to increase serum FSH, LH, and testosterone levels (p < 0.05). In addition, sperm density, motility, and progressive movement were observed to increase in asprosin administered groups (p < 0.05). This study suggests that central asprosin stimulate the HPT axis and also epididymis tissue. Our results implicates potential role for asprosin in male infertility.Öğe Investigation of the antitumor effects of anti-inflammatory desloratadine and trimebutine on different types of human cancer cells: An in vitro study(2023) Özkaya, Semiha Nur; Keskin, Tuba; Tekin, Çiğdem; Tekin, Suat; Beytur, AliAim: Inflammation is a process associated with the development and progression of cancer. Desloratadine (DES) and Trimebutin (TMB) are anti-inflammatory agents used in the treatment of various diseases. This study aimed to investigate the antitumor effects of DES and TMB, which exhibit anti-inflammatory effects, on different human cancer cell lines. Materials and Methods: In this study, human prostate (LNCaP), ovarian (A2780), breast (MCF-7) and colon cancer (Caco-2) cell lines were treated with DES and TMB at concentrations of 1, 5, 25, 50 and 100 µM. Cells were treated with the compounds for 6, 12, and 24 hours, and the change in cell viability was determined using the 3- (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The inhibitory concentration 50 (LogIC50) values of the compounds were calculated using GraphPad Prism 8 software based on cell viability results. The genotoxic effects of the compounds on cells were determined using the comet assay. Group comparisons were performed using the Kruskal-Wallis H test and p<0.05 was considered significant. Results: Exposure of LNCaP, A2780, MCF-7, and Caco-2 cells to DES and TMB agents for 6, 12 and 24 hours significantly reduced cell viability (p<0.05). According to the comet assay results, DES and TMB caused significant DNA damage in the cell lines (p<0.05). Conclusion: The study results demonstrate that DES and TMB, which have anti inflammatory effects, exert cytotoxic effects by inducing DNA damage in cancer cells.Öğe Investigation of the Effect of Irisin Hormone on the Viability of Hypothalamic Cell Lines(Wiley, 2023) Ercan, Eda Coban; Keskin, Tuba; Ucer, Aysun; Canpolat, Sinan[Abstract Not Available]Öğe Mitochondrial-Derived Peptide (MOTS-c) Induces Cell Death by Inducing DNA Damage in Caco-2 Cell Line(Wiley, 2022) Erden, Yavuz; Gunay, Sevilay; Tekin, Suat; Celebioglu, Hasan Ufuk; Tekin, Cigdem; Navruz, Fahriye Zemheri; Keskin, Tuba[Abstract Not Available]Öğe New sulfa drug derivatives and their zinc(II) complexes: synthesis, spectroscopic properties and in vitro cytotoxic activities(Taylor & Francis Inc, 2024) Sahal, Hakan; Oz, Samet; Keskin, Tuba; Tekin, Suat; Canpolat, Erdal; Kaya, MehmetSynthesis of four sulfa drug derivatives (L-1-L-4) and Zn(II) complexes derived from sulfonamide group antibiotic substances was carried out using the hydrothermal technique (HT) and their structures of the obtained compounds were explained using elemental analysis (EA), FT-IR and NMR (H-1- and C-13-). Cytotoxic activities of four novel sulfa drug based-Schiff base compounds and their Zn(II) complexes were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay using MCF-7 (human breast cancer), Caco-2 (human colorectal adenocarcinoma), A2780 (human ovarian cancer) and LNCaP (human prostate adenocarcinoma) cell lines. LogIC50 values of all obtained compounds were computed with the Graphpad Prism 6 program after 24 h of treatment for MCF-7, Caco-2, A2780 and LNCaP cells. Comet assay experiments were performed using LogIC(50) concentrations of all compounds to determine DNA damage. Based on the data obtained, all compounds significantly decreased MCF-7, Caco-2, A2780 and LNCaP cell viability compared to the control groups (p < 0.05). Communicated by Ramaswamy H. SarmaÖğe Silyl-functionalised silver rhodium and iridium N-heterocyclic carbene complexes with promising cytotoxicity and antimicrobial potential(Elsevier, 2022) Karatas, Mert Olgun; Keskin, Tuba; Ozdemir, Namik; Kucukbay, Hasan; Tekin, Suat; Mansur, Ahmet; Gunal, SelamiIn the present study, we have synthesized Ag, Rh, and Ir complexes of a silyl-substituted NHC ligand in order to investigate their biological properties. Firstly, Ag-NHC (2) complex was synthesized by the interaction of Ag2O with benzimidazolium iodide (1). Rh-(3) and Ir-NHC (4) complexes were synthesized by the transmetalation reaction between Ag-NHC and [M(cod)Cl]2 (M = Rh or Ir) dimers. The complexes were characterised by NMR spectroscopy and elemental analyses, and crystal structures of Rh-and Ir-NHC (3 and 4) were also reported. The cytotoxic effects of the complexes were tested on four human cancer cell lines and the antimicrobial activities of the complexes were tested against a panel of nine pathogenic microorganisms. The complexes performed com-parable or better activity than standards, cisplatin and AgNO3.Öğe Trimebutin Demonstrate Antitumor Effects on Different Types of Human Cancer Cells(Wiley, 2023) Keskin, Tuba; Tekin, Suat; Tekin, Cigdem; Beytur, Ali[Abstract Not Available]
