Yazar "Keskin, Tuba" seçeneğine göre listele
Listeleniyor 1 - 20 / 20
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe Asprosin protects against ischemia/reperfusion-induced kidney injury in mice(Springer, 2025) Keskin, Tuba; Korkmaz, Engin; Yildiz, Azibe; Tekin, Cigdem; Beytur, Ali; Tekin, SuatIschemia-reperfusion (IR)-induced acute kidney injury (AKI) is a complex pathophysiological process involving inflammation, oxidative stress, and apoptosis. Asprosin (ASP), a fasting-induced glucogenic hormone, has been shown to influence oxidative and apoptotic pathways in various tissues. This study investigated the potential renoprotective effects of ASP in a murine model of IR-induced AKI. Thirty-two male Balb/c mice were randomly assigned to four groups (n = 8): Control, IR, ASP1 (1 mu g/kg ASP), and ASP10 (10 mu g/kg ASP). While the control group received no treatment. Vehicle and ASP (1 or 10 mu g/kg) were administered intravenously five minutes before ischemia to the IR and ASP-treated groups, respectively. Renal ischemia was induced for 22 min, followed by a 24-h reperfusion period. Renal function markers, inflammatory cytokines, oxidative stress parameters, and caspase-3 expression were evaluated. Histopathological alterations were assessed using hematoxylin-eosin staining. IR significantly increased BUN, creatinine, IL-1 beta, TNF-alpha, MDA levels, and caspase-3 expression, while reducing antioxidant enzymes (SOD, CAT). ASP pretreatment effectively reversed these changes (p < 0.05), as reflected by improved renal function, reduced inflammation and oxidative stress, and decreased apoptotic activity. These functional and molecular improvements were also supported by histological evidence showing reduced kidney damage following ASP treatment. Collectively, the findings suggest that ASP protects against IR-induced AKI by alleviating inflammation, oxidative stress, and apoptosis.Öğe Asprosin Takes a Protective Role in Mice Established Experimental Acute Kidney(Wiley, 2023) Korkmaz, Engin; Keskin, Tuba; Yildiz, Azibe; Tekin, Cigdem; Tekin, Suat; Beytur, Ali[Abstract Not Available]Öğe Central Application of Asprosin Increases Secretion of Male Reproductive Hormones: An In Vivo Study(Wiley, 2022) Keskin, Tuba; Erden, Yavuz; Tekin, Suat[Abstract Not Available]Öğe Eco-friendly synthesis of α-aminophosphonates using ultrasound irradiation: In vitro pharmacological studies and molecular docking approach(Elsevier, 2026) Badaoui, Kawther; Sehout, Imene; Sekerci, Guldeniz; Keskin, Tuba; Tekin, Suat; Hamlaoui, Ikram; Kucukbay, HasanA series of novel alpha-aminophosphonates 4a-m have been successfully synthesized via Kabachnik-Fields reaction under ultrasound irradiation and in the presence of acetylsalicylic acid as catalyst with yields ranging 69-87 %. All compounds were characterized by 1H, 13C, 31P, FTIR and elemental analysis. Anticancer activity was evaluated using three cancer cell lines (MCF-7, A2780, Caco-2). Compounds 4k, 4h and 4e exhibited good cytotoxic activity against MCF-7. The in vitro antioxidant activity of the synthesized molecules was evaluated by four complementary methods (DPPH, ABTS, FRAP and Phenanthroline). Compound 4c and 4f showed high antioxidant capacity at ABTS scavenging assay, while compounds 4a, 4b, 4c, 4d, 4e and 4f showed high antioxidant capacity at Phenanthroline assay. The target molecules showed moderate antifungal activity against the phytopathogenic strain Fusarium oxysporum f.sp. lycopersici (FOL). Compounds 4e, 4h, and 4k, that showed the best the highest activity against breast cancer (MCF-7), were further analyzed through molecular docking to investigate their modes of interaction with two enzymes implicated in this cancer.Öğe Erkek sıçanlara intraserebroventriküler asprosin uygulamasının üreme hormonları üzerine etkileri(İnönü Üniversitesi, 2021) Keskin, TubaAmaç: Asprosin beyaz yağ dokudan salgılanan ve hepatik glikoz salınımını arttıran protein yapılı bir hormondur. Asprosinin beyin ve testis dokusunda lokalize olduğu, bununla birlikte asprosin uygulamasının spermlerin ilerleyici hareketini arttırdığı bilinmektedir. Bu çalışma, merkezi asprosin infüzyonunun hipotalamus hipofiz testiküler aksında görev alan hormonlar ile sperm hücreleri üzerindeki etkilerini araştırmak amacıyla yapıldı. Materyal ve Metot: Çalışmada 40 adet Spraque Dawley ırkı erkek sıçan kullanıldı. Sıçanlar kontrol, sham, 34 ve 68 nM asprosin olmak üzere dört gruba ayrıldı (n=10). Kontrol grubuna herhangi bir uygulama yapılmazken sham ve deney gruplarındaki sıçanların lateral ventriküllerine beyin infüzyon kiti implant edildi ve 14 gün süresince infüzyon (Sham grubu için yapay beyin omurilik sıvısı, uygulama grupları için 34 ve 68 nM asprosin) gerçekleştirildi. Deney sonunda sıçanların hipotalamus, kan ve epididimis dokuları toplandı. Hipotalamus dokusunda RT-PCR ve Western Blot yöntemleriyle GnRH mRNA ve doku protein düzeyleri belirlendi. Kan örneklerinden ELISA yöntemi kullanılarak serum LH, FSH ve testosteron seviyeleri, epididimis dokusunda ise sperm hücreleri incelendi. Bulgular: Asprosin uygulanan grupların GnRH mRNA ve protein ifadeleri kontrol ve sham gruplarına kıyasla yüksekti (p<0.05). Asprosin infüzyonunun serum FSH, LH ve testosteron seviyelerini de arttırdığı belirlendi (p<0.05). Ayrıca asprosin uygulanan gruplarda sperm yoğunluğunun, hareketliliğinin ve ileri hareketinin arttığı görüldü (p<0.05). Sonuç: Bu çalışma merkezi olarak uygulanan asprosinin hipotalamus hipofiz testiküler aksta görev alan hormonlar ve epididimis dokusu üzerinde aktive edici özelliğe sahip olabildiğini ortaya koymaktadır. Elde edilen sonuçlar asprosinin infertilite tedavisinde ele alınması gereken önemli bir protein/biyobelirteç olduğunu düşündürmektedir. Anahtar Kelimeler: Asprosin, FSH, GnRH, LH, Testosteron, Sperm.Öğe Green Extraction Enhances Antioxidant and Cytotoxic Properties of Pomegranate By-Products Against Human Cancer Cell Lines(Springer, 2025) Aydin, Merve; Akbulut, Sumeyye; Keskin, Tuba; Tontul, Ismail; Turker, Selman; Sandal, SuleymanThe aim of this study was to determine the anticancer effects of extract obtained from pomegranate by-products by green extraction (ultrafiltration and enzymatic extraction) (GE) on prostate (LNCaP), ovarian (A2780), breast (MCF-7), and colon (Caco-2) cancer cell lines compared to the traditional extraction (TE) and pomegranate juice (PJ). The GE sample demonstrated the highest total or individual phenolic content, and antioxidant activity. The major phenolics in all samples were determined to be ellagic acid, punicalagin A and punicalagin B. The 50% and 100% doses of the PJ exhibited a significant reduction in the viability of all cancer cells except LNCaP cells (only 100%) (p < 0.05). The TE sample showed a significant decrease in the viability of all cancer cells except the MCF-7 cell line (at all doses) at doses other than 1 M (p < 0.05). The GE sample caused a significant decrease in all cancer cells at all doses (1, 5, 25, 50, 100 and 150 M) (p < 0.05). Consequently, the GE sample applied with the green method was effective at all doses in reducing the viability of cancer cells.Öğe Intracerebroventricular asprosin administration strongly stimulates hypothalamic-pituitary-testicular axis in rats(Elsevier Ireland Ltd, 2021) Keskin, Tuba; Erden, Yavuz; Tekin, SuatAsprosin, a protein-based secretary product of white adipose tissue, stimulates appetite hepatic glucose production. It crosses blood-brain barrier and stimulates appetite center and causes sperm chemotaxis but exact role of this endogenous agent is not completely known. This study was conducted to investigate possible effects of central asprosin infusion on the hormones involved in the hypothalamic-pituitary-testicular (HPT) axis and sperm cells. Spraque Dawley male rats were divided into four groups; control, sham, low asprosin (34) and high asprosin (68 nM) groups, (n = 10 for each group). Control group remain intact while a brain infusion kit was placed in the lateral ventricles of the rats in the sham group (artificial cerebrospinal fluid) and asprosin (34 and 68 nM) was infused for 14 days. At the end of the experiment, the hypothalamus, blood, and epididymis tissues of the rats were collected. Gonadotropin-releasing hormone (GnRH) mRNA and tissue protein levels were determined in the hypothalamus tissue by RT-PCR and Western Blot methods. Serum luteinizing hormone (LH), follicle-stimulating hormone (FSH), and testosterone levels were examined using the ELISA method from blood samples and sperm cells were examined in the epididymis tissue. GnRH mRNA and protein expressions of asprosin administered groups were higher than control and sham groups (p < 0.05). Asprosin infusion was also found to increase serum FSH, LH, and testosterone levels (p < 0.05). In addition, sperm density, motility, and progressive movement were observed to increase in asprosin administered groups (p < 0.05). This study suggests that central asprosin stimulate the HPT axis and also epididymis tissue. Our results implicates potential role for asprosin in male infertility.Öğe Investigating the Effects of Diisobutyl Phthalate Chemical on Feeding Behavior and Obesity(Wiley, 2025) Beyoglu, Elif Maide; Keskin, Tuba; Tanbek, Kevser; Sandal, Suleyman[No abstract available]Öğe Investigation of In Vitro Cytotoxic and Genotoxic Properties of New Dioxybiphenyl-bridged Spiro Cyclotrifosphazene with Dihydroxycoumarin Groups Synthesized by the Click Method(Wiley, 2025) Keskin, Tuba; Savasci, Zeynep; Seker, Ferhan Sultan; Cetiner, Rumeysa; Biryan, Fatih; Koran, Kenan; Tekin, Suat[No abstract available]Öğe Investigation of the antitumor effects of anti-inflammatory desloratadine and trimebutine on different types of human cancer cells: An in vitro study(2023) Özkaya, Semiha Nur; Keskin, Tuba; Tekin, Çiğdem; Tekin, Suat; Beytur, AliAim: Inflammation is a process associated with the development and progression of cancer. Desloratadine (DES) and Trimebutin (TMB) are anti-inflammatory agents used in the treatment of various diseases. This study aimed to investigate the antitumor effects of DES and TMB, which exhibit anti-inflammatory effects, on different human cancer cell lines. Materials and Methods: In this study, human prostate (LNCaP), ovarian (A2780), breast (MCF-7) and colon cancer (Caco-2) cell lines were treated with DES and TMB at concentrations of 1, 5, 25, 50 and 100 µM. Cells were treated with the compounds for 6, 12, and 24 hours, and the change in cell viability was determined using the 3- (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The inhibitory concentration 50 (LogIC50) values of the compounds were calculated using GraphPad Prism 8 software based on cell viability results. The genotoxic effects of the compounds on cells were determined using the comet assay. Group comparisons were performed using the Kruskal-Wallis H test and p<0.05 was considered significant. Results: Exposure of LNCaP, A2780, MCF-7, and Caco-2 cells to DES and TMB agents for 6, 12 and 24 hours significantly reduced cell viability (p<0.05). According to the comet assay results, DES and TMB caused significant DNA damage in the cell lines (p<0.05). Conclusion: The study results demonstrate that DES and TMB, which have anti inflammatory effects, exert cytotoxic effects by inducing DNA damage in cancer cells.Öğe Investigation of the Effect of Irisin Hormone on the Viability of Hypothalamic Cell Lines(Wiley, 2023) Ercan, Eda Coban; Keskin, Tuba; Ucer, Aysun; Canpolat, Sinan[Abstract Not Available]Öğe Investigation of the Effects of Vildagliptin on Cancer Cell Lines: An In Vitro Study(Wiley, 2025) Sekerci, Guldeniz; Keskin, Tuba; Tekin, Suat[No abstract available]Öğe Loratadine Reduces Cell Viability in Different Types of Human Cancer Cell Lines(Wiley, 2025) Keskin, Tuba; Sekerci, Guldeniz; Tekin, Suat[No abstract available]Öğe Microwave Synthesis, Evaluation, and Docking Study of Amino Acid Derivatives of 7-Chloroquinoline: Exploring Cytotoxic and Antioxidant Potentials(Amer Chemical Soc, 2026) Ezugwu, James A.; Kucukbay, Fatumetuzzehra; Oz, Samet; Keskin, Tuba; Boulebd, Houssem; Tekin, Suat; Kucukbay, HasanNew carbamate and amino acid derivatives of 7-chloroquinoline were synthesized and characterized using FTIR, 1H NMR, 13C NMR, and HRMS analysis. The synthesized compounds were obtained through a benzotriazole-mediated approach via microwave synthesis and evaluated for antioxidant and anticancer activities. All the synthesized compounds have antioxidant properties though less than those of the standard. Cytotoxic activities of new 7-chloroquinolinyl benzyl amino carbamate derivatives were accessed using LNCaP (Lymph Node Carcinoma of the Prostate), A2780 (human ovarian cancer), and MCF-7 (breast cancer) cell lines. For cytotoxicity research, the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used. The synthesized compounds were subjected to a cell viability assay, and following a 24 h treatment, the IC50 values were determined. Among all the tested compounds, compound 4b demonstrated comparable antitumor activity against LNCaP, A2780, and MCF-7 cell lines when compared to the standard drug docetaxel with IC50 values of 6.61, 2.81, and 5.69 mu g/mL for LNCaP, A2780, and MCF-7 cell lines, respectively. A molecular docking study targeting the beta-tubulin enzyme revealed that compounds 4a, 4b, and 5b exhibit a high affinity for the taxane binding site and may mimic the action of docetaxel.Öğe Mitochondrial-Derived Peptide (MOTS-c) Induces Cell Death by Inducing DNA Damage in Caco-2 Cell Line(Wiley, 2022) Erden, Yavuz; Gunay, Sevilay; Tekin, Suat; Celebioglu, Hasan Ufuk; Tekin, Cigdem; Navruz, Fahriye Zemheri; Keskin, Tuba[Abstract Not Available]Öğe New sulfa drug derivatives and their zinc(II) complexes: synthesis, spectroscopic properties and in vitro cytotoxic activities(Taylor & Francis Inc, 2024) Sahal, Hakan; Oz, Samet; Keskin, Tuba; Tekin, Suat; Canpolat, Erdal; Kaya, MehmetSynthesis of four sulfa drug derivatives (L-1-L-4) and Zn(II) complexes derived from sulfonamide group antibiotic substances was carried out using the hydrothermal technique (HT) and their structures of the obtained compounds were explained using elemental analysis (EA), FT-IR and NMR (H-1- and C-13-). Cytotoxic activities of four novel sulfa drug based-Schiff base compounds and their Zn(II) complexes were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay using MCF-7 (human breast cancer), Caco-2 (human colorectal adenocarcinoma), A2780 (human ovarian cancer) and LNCaP (human prostate adenocarcinoma) cell lines. LogIC50 values of all obtained compounds were computed with the Graphpad Prism 6 program after 24 h of treatment for MCF-7, Caco-2, A2780 and LNCaP cells. Comet assay experiments were performed using LogIC(50) concentrations of all compounds to determine DNA damage. Based on the data obtained, all compounds significantly decreased MCF-7, Caco-2, A2780 and LNCaP cell viability compared to the control groups (p < 0.05). Communicated by Ramaswamy H. SarmaÖğe Ramelteon Exhibits Anticancer Effects in Different Types of Human Cancer Cells(Wiley, 2025) Turkol, Senanur; Keskin, Tuba; Sekerci, Guldeniz; Tekin, Cigdem; Tekin, Suat[No abstract available]Öğe Ramelteon Reduces Oxidative Damage in Diabetic Mice(Wiley, 2025) Keskin, Tuba; Tekin, Suat[No abstract available]Öğe Silyl-functionalised silver rhodium and iridium N-heterocyclic carbene complexes with promising cytotoxicity and antimicrobial potential(Elsevier, 2022) Karatas, Mert Olgun; Keskin, Tuba; Ozdemir, Namik; Kucukbay, Hasan; Tekin, Suat; Mansur, Ahmet; Gunal, SelamiIn the present study, we have synthesized Ag, Rh, and Ir complexes of a silyl-substituted NHC ligand in order to investigate their biological properties. Firstly, Ag-NHC (2) complex was synthesized by the interaction of Ag2O with benzimidazolium iodide (1). Rh-(3) and Ir-NHC (4) complexes were synthesized by the transmetalation reaction between Ag-NHC and [M(cod)Cl]2 (M = Rh or Ir) dimers. The complexes were characterised by NMR spectroscopy and elemental analyses, and crystal structures of Rh-and Ir-NHC (3 and 4) were also reported. The cytotoxic effects of the complexes were tested on four human cancer cell lines and the antimicrobial activities of the complexes were tested against a panel of nine pathogenic microorganisms. The complexes performed com-parable or better activity than standards, cisplatin and AgNO3.Öğe Trimebutin Demonstrate Antitumor Effects on Different Types of Human Cancer Cells(Wiley, 2023) Keskin, Tuba; Tekin, Suat; Tekin, Cigdem; Beytur, Ali[Abstract Not Available]
