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Öğe The need for confirmation in coliform and E-coli enumeration in foods(Scientific Technical Research Council Turkey, 2002) Çakir, I; Dogan, HB; Baspinar, E; Keven, F; Halkman, AKFor food and environmental analysis, coliform bacteria and especially E. coli should be determined and enumerated rapidly, correctly and economically. Even if the results are obtained either by traditional or improved techniques, the results require confirmation, meaning that these techniques are not reliable. Confirmation tests need additional time and/or cost. In this study, 500 food samples of 10 various types were analyzed for their natural coliform contamination by the standard MPN method and E. coli by two MUG based MPN techniques. Enumeration results were statistically analyzed to determine whether confirmation tests for coliform and E. coli analysis are necessary or not according to the results of three statistical reliability analyses: Pearson's correlation coefficient (r), Cronbach's alpha (alpha) and determination coefficient (r(2)). The results clearly showed that BGB broth confirmation for LST broth in coliform analysis and indole test confirmation for MUG test in E. coli analysis are not necessary (p < 0.0001).Öğe Relationship among fecal coliforms and Escherichia coli in various foods(Springer, 2003) Dogan-Halkman, HB; Çakir, I; Keven, F; Worobo, RW; Halkman, AKFor much of the twentieth century, coliform bacteria and especially Escherichia coli have been used as indicators of possible post-processing contamination and the presence of E. coli as an indicator of fecal contamination in foods. In this study, 500 foods in 10 different groups, mainly dairy products, delicatessen products, salads, spices, cream cakes and fresh fruit and vegetable samples, were analyzed for the natural contamination of fecal coliforms and E. coli by the standard most probable number (MPN) method. The difference between weighted means of fecal coliforms and E. coli counts were only 0.246 log(10) MPN/g-ml (MPN/gram for solid samples, and MPN/milliliter for liquids). Enumeration results were also evaluated by Pearson's correlation coefficient (r), Cronbach's alpha (alpha) and determination coefficient (r(2)) analysis. According to results, although 33 samples contained only non-E. coli fecal coliforms, the results of reliability analyses indicated that fecal coliform counts and E. coli counts may be used interchangeably (P<0.0001). It can be said that fecal coliform or, preferably E. coli analysis is sufficient for rapid routine determination of fecal contamination, at least for those food groups analyzed in this research.











