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    Analyses of the relationships among biofilm activity, antibiotic resistance and expressions of SHV, TEM, CTX and IntI in Klebsiella pneumoniae
    (2021) Guckan, Ridvan; Kilinc, Cetin; Yazgan, Burak; Baskan, Ceren; Mesci, Seda; Yildirim, Tuba
    Aim: Klebsiella pneumoniae as an opportunistic pathogen is responsible for nosocomial, urinary tract infections, pneumonia, bacteremia, liver abscesses, and respiratory and blood infections in patients. The aim of our study is to examine the antibiotic sensitivity, expression levels of IntI, TEM, SHV and CTX genes, which play a role in multidrug resistance, biofilm formation and even the relationships among them. Materials and Methods: Identification was implemented by carrying out the VITEK2 system and phenotypic confirmation of the ESBL producing isolates by using a combined disc test. Antibiotic sensitivity was implemented by disc diffusion method and microdilution methods toward various antibiotics. Furthermore, biofilm formation was examined through microtitration plate method. Results: The expressions of IntI, TEM, SHV and CTX were implemented in qRT-PCR. 19 were susceptible to all antibiotics, 17 were ESBL (+) and 16 were carbapenem-resistant among 52. The expression of these genes was upregulated in ESBL (+) strains and produced biofilm and the expression of these genes was upregulated, too. Conclusion: The results of the study revealed the potential role of the mentioned genes in biofilm formation and antibiotic resistance, enabling the development of new drugs. Further studies will be essential so as to determine when or how these systems are included in antibiotic resistance and biofilm formation.
  • Küçük Resim Yok
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    Sociodemographic characteristics of patients with Demodex brevis and Demodex folliculorum infestation and its association with rosacea and Behcet's disease.
    (Allied Acad, 2015) Durmaz, Suleyman; Yula, Erkan; Kaya, Ozlem Aycan; Gokmen, Aysegul Aksoy; Kilinc, Cetin; Atambay, Metin; Ekiz, Ozlem
    Demodex mites are ectoparasites often found in the skin thought to be associated with the development of some skin diseases. Demodex positivity may be affected by several factors. In order to determine the relationship between Demodex infestation and rosacea disease (RD), Behcet's disease (BD) and various individual parameters such as gender, personal hygiene and skin type. A total of 238 individuals (61 with RD, 46 with BD, and 131 healthy subjects) were investigated for the presence of Demodex folliculorum and D. brevis using the standardized skin surface biopsy technique and a questionnaire. The incidence of Demodex mites in RD patients was significantly higher when compared to the control group (p=0.010 and p=0.001, respectively). However, there was no difference between those with BD and the control group. There was a significant difference between ages of groups, skin types, use of moisturizer, and itching, without taking into account the presence of Demodex among the disease groups (p<0.05). On the other hand, there was no significant difference between disease groups in terms of data of gender, skin care, epilation, and face washing. Our study reveals that the positivity rates of Demodex mites in rosacea patients were significantly higher. Mean ages of groups, skin types, use of moisturizer, and the presence of itching of the ear and face may be associated with higher density of Demodex mites.