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    Investigating inhibitory effects of punica granatum fruit extracts on lipid peroxidation in the fenton reagent environment
    (Chemical Publishing Co., 2012) Ozsahin A.D.; Kirecci O.A.; Yilmaz O.; Erden Y.; Bircan B.; Karaboga Z.
    In this study, it is aimed to find antioxidant activity of methanolic extract of Punica granatum fruit, by determining a,a-diphenyl-b- picrylhydrazyl (DPPH) radical scavenging property and also by determining the capacity to prevent the formation of lipid peroxidation which is found in in-vitro environment that soybean and corn oils are found. The capacity of preventing lipid peroxidation was analyzed in an in vitro environment that oil samples were existed by evaporating methanol phase of extracts. In the experiment, the groups were formed as control group e.g., Fenton reactive, Pomegranate extract + Fenton reactive. It was observed that the lipid peroxidation level formed on the oils had increased at a significant level when compared to Fenton reactive groups according to the control group. When the extract groups are compared (to each other) according to the Fenton reactive group, it was detected that lipid peroxidation level had significantly decreased (p > 0.0001). According to the scavenging activity of ?,?-diphenyl-?-picrylhydrazyl radical, it was determined that the pomegranate extracts were effective at their highest level at 250-500 ?L (p < 0.0001). It was concluded that Punica granatum effectively decreases the lipid peroxidation level, neutralizes the ?,?-diphenyl-?-picrylhydrazyl free radical and has a characteristic of conserving the fatty acid content.
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    Radical scavenging activities of some Vitis vinifera varieties grown in malatya (Turkey) and their preventive effects on the formation lipid peroxidation in unsaturated fatty acids mediated
    (Chemical Publishing Co., 2010) Ozsahin A.D.; Yilmaz O.; Tuzcu M.; Kirecci O.A.; Guvenc M.
    This study examined the phytochemical characteristics of five samples of Vitis vinifera grown in the Malatya region of Turkey and investigated their impacts on human health by determining their antioxidant activities in vitro. The present research includes five native grape varieties (white: Tahanebi, Sam and Kureys; red: Köhnü; black: Banazi Karasi). Antioxidative activities of the grape extracts were determined with the following modifications. The free radical scavenging effect in extracts was assessed by the decolouration of a methanolic solution of DPPH. Lipid peroxidation level was found to be significantly high in the Fenton reagent (FR) containing group when compared to the control group (p < 0.0001). However, lipid peroxidation level decreased significantly in the groups to which grape extracts were added (p < 0.0001). According to DPPH results, all grape samples showed effective antioxidant activity in different concentrations and these activities were observed to change in parallel with the increase in flavonoid concentration. The results indicate that the grape fruit extracts decrease lipid peroxidation level in the Fenton reagent environment and have scavenging effect on the DPPH depending on grape varieties.
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    The relationship between nitric oxide and plant hormones in SNP administrated sunflower plants under salt stress condition
    (Wydawnictwo Akad Rolniczej W Lublinie, 2016) Yurekli F.; Kirecci O.A.
    Nitric oxide (NO) and sodium nitro prusside (SNP) are striking molecules and play important roles in animals and plants. SNP serves as nitric oxide donor in both group. NO can act free radical and impaires important biomolecules functions beside this it has beneficial effect recovery from salinity, drought etc. NO and SNP are beneficial and protectant molecules in cope with stressfull conditions. In plants these molecules are very important, and regulate many physiological events. In the present study, endogen abscisic acid (ABA), indole acetic acid (IAA), gibberellic acid (GA3) and NO levels were investigated in NaCl, SNP and plant growth regulators treated sunflower plant (Helianthus annuus L.) leaves and roots. The content of NO was higher in GA3 + SNP treated plant leaves at 72 h. The highest IAA level was determined in IAA + SNP treated plant roots at 72 h. In NaCl + ABA treated plant leaves ABA was higher at 72 and GA3 levels were equal or less than the control group. Our results showed that coadministration of SNP and plant growth regulators cope with salinity stress via elevated internal hormone and NO level versus salinity. © Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie, Lublin 2016.