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Öğe Essential oil chemical composition, antimicrobial, anticancer, and antioxidant effects of Thymus convolutus Klokov in Turkey(Walter De Gruyter Gmbh, 2021) Yuceturk, Selma Celen; Turkoglu, Sumeyye Aydogan; Kockar, Feray; Kucukbay, F. Zehra; Azaz, A. DilekIn this study, the chemical composition, antimicrobial, antioxidant, and anticancer effects of Thymus convolutus Klokov oil and its main compound camphor were investigated. The oil was isolated from T. convolutus using hydrodistillation method, analyzed by gas chromatography/mass spectrometry (GC-MS), and 66 compounds were identified. The main component was determined as camphor at 16.6%. The antioxidant properties were identified with the DPPH (2,2'-diphenyl-1-picrylhydrazyl) radical-scavenging method and, 33.39 +/- 0.25% DPPH was scavenging in 1000 mu g/mL of essential oil. The strong antimicrobial activity was observed against Escherichia coli, Enterobacter aerogenes, Proteus vulgaris, and Pseudomonas aeruginosa with MIC values of 125 mu g/mL. Aspergillus flavus was more sensitive (28%) against T. convolutus essential oil than other fungi. The cytotoxic effect of oil was analyzed by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) method. Camphor was effective on human hepatoma cells (Hep3B) at concentrations of 1 mg/mL, 500, 250, and 125 mu g/mL, while essential oil of T. convolutus was found to be effective at concentrations of 250 and 125 mu g/mL. A reduction in cell proliferation was observed in colon carcinoma cells (HT-29) treated with 500 mu g/mLcamphor for 48 h. No statistically significant effect was found in Umbilical Vein Endothelial Cells (HUVEC) treated with essential oil and camphor.Öğe Is phenytoin a safe agent for staple line recovery after gastric sleeve surgery in rats?(Turkish Assoc Trauma Emergency Surgery, 2023) Gay, Ferhat; Duran, Ali; Tokay, Esra; Hacioglu, Nelin; Kockar, Feray; Altun, Eren; Kanat, Burhan HakanBACKGROUND: The most challenging and mortal complication of gastric sleeve surgery (SG) is staple line leakage. Although many agents have been used for increasing tissue healing on the stapler line, there is still no consensus on its effectiveness and efficacy. The aim of study is to determine the effect of phenytoin on the healing process of gastric sleeve surgery in rats. METHODS: On the 10th post-operative day, the effects of phenytoin on bursting pressure in the stapler line were evaluated alongside pathohistological examinations. To investigate the molecular impact of phenytoin on the expression of TGF-8, VEGF, FGF2, and p53 genes, quantitative real-time polymerase chain reaction was utilized. In addition, gene expressions at the protein level were determined by immunohistochemical analysis. RESULTS: No signs of intra-abdominal leakage were observed in the resected samples. A statistically essential extend in stable bursting pressure measure was observed between the control group and the group treated with phenytoin application. Pathohistological results indicate that the mean score of collagens of the study group (3.2 +/- 0.42) was significantly higher than the control group (2.3 +/- 0.48) (P=0.003). In addition, the mean epithelization score of the study group (3.4 +/- 0.52) was significantly higher than the control group (2.1 +/- 0.57) (P=0.001). mRNA of TGF8, FGF2, VEGF, and p53 genes drastically increased phenytoin treated group. High FGF2 protein expression levels were determined from phenytoin use compared to the control group. CONCLUSION: Molecular studies suggest that phenytoin may increase the healing process of Gastric sleeve following SG in rats and may become a new agent for the prevention of human gastric leaks.