Yazar "Kuşkucu, Mert Ahmet" seçeneğine göre listele

Listeleniyor 1 - 2 / 2
  • Küçük Resim
    Öğe
    Investigation of carbapenem resistance and the first identification of Klebsiella pneumoniae carbapenemase KPC enzyme among Escherichia coli isolates in Turkey A prospective study
    (Travel Medicine and Infectious Disease, 2016) Kuşkucu, Mert Ahmet; Karakullukçu, Asiye; Ailiken, Maılıhaba; Otlu, Barış; Mete, Birgül
    Background: The aim of this study was to determine the presence of carbapenem resistance and carbapenemase production in Escherichia coli isolates from clinical samples in Turkey. Methods: The prospective study included a total of 4.052 Escherichia coli isolates collected from patients admitted to a hospital from March 2011 to May 2012. We used ertapenem disc for screening carbapenemase production, and the confirmation was performed by using Etest. The resistance mechanisms and genetic relatedness of the carbapenem resistant strains were investigated by using PCR (polymerase chain reaction) and pulsed-field gel electrophoresis (PFGE), respectively. Results: Among the 4.052 E. coli isolates, 24 (0.59%) were found to be carbapenem resistant. Of these, only 5 isolates were positive for OXA-48 and 2 isolates were positive for Klebsiella pneumoniae carbapenemase (KPC)-2. The KPC-2 producing E. coli strains (n ¼ 2) were both isolated from the same patient. The blaKPC genes were confirmed using DNA sequence analysis. The genetic relationship between the 24 E. coli strains studied by PFGE revealed that the strains were genetically unrelated. Conclusions: This article confirms, to our knowledge for the first time, the detection of KPC-2-producing E. coli in Turkey, with OXA-48 being the most frequent carbapenemase in the study.
  • Küçük Resim Yok
    Öğe
    Six year distribution pattern of hepatitis C virus in Turkey a multicentre study
    (Biotechnology & Biotechnological Equipment, 2015) Altındiş, Mustafa; Dal, Tuba; Akyar, Işın; Karatuna, Onur; Gökahmetoğlu, Selma; Tezcan Ülger, Seda; Külah, Canan; Uzun, Berrin; Şener, Aslı Gamze; Özdemir, Mehmet; Aydoğan, Sibel; Kuşkucu, Mert Ahmet; Midilli, Kenan; Otlu, Barış; Celen, Mustafa Kemal; Buruk, Kurtuluş; Güdücüoğlu, Hüseyin
    Hepatitis C infection is a public health problem. The aim of this retrospective study was to determine the distribution of hepatitis C virus (HCV) genotypes in seven regions of Turkey, by evaluating 7002 patients with chronic HCV in a six-year period. During the 2009 2014 period, serum/plasma samples from 7002 new consecutive HCV RNA positive patients were collected. The female patients were 3867 (55.2%). The genotype distribution of HCV patiens was evaluated by ages and years. Statistical analysis was performed by using the Mann Whitney test and the x2 analysis. During the six-year period, genotype 1b was the most common genotype (67.7%) followed by untypeable genotype 1 (7.7%), genotype 4 (7.3%) and genotype 3 (6.7%). In 2014, genotype 3 was the second most common one (11.3%) and genotype 4 was the third most common one (9.8%). In the group with <25 years old patients, genotype 1b was most common (78.48%, 62/79) between the years of 2009 and 2011, whereas genotype 3 (34.8%, 86/247), between the years of 2012 and 2014. Genotype 1b was the most common in the groups between 26 and 35 years, 36 and 45 years, 46 and 55 years, 56 and 65 years. The rate of genotype 3 was increased from 4.78% to 10.06% and the rate of genotype 4 was increased from 1.3% to 3.84%, from 2009 2011 to 2012 2014. In recent years, genotypes 3 and 4 have gained importance. New therapeutic strategies and survey studies may be required for the modified HCV genotype pattern.