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    Genotoxic effects of banding procedure with different orthodontic cements on human oral mucosa cells
    (Tubitak Scientific & Technological Research Council Turkey, 2012) Ozturk, Firat; Yuksel, Sengul; Toy, Ebubekir; Kurtoglu, Elcin Latife; Kucuk, Eyup Burak
    Aim: To assess the genotoxic and cytotoxic effects of banding procedure with 5 different orthodontic cements on human oral buccal epithelium cells. Materials and methods: Fifty healthy volunteers (mean age: 14.54 +/- 2.37 years) were randomly divided into 5 groups of 10. Preformed stainless steel molar bands (3M Unitek) were cemented to the upper and lower first molar teeth with 5 different orthodontic cements (Durelon, GC Fuji Ortho Band LC Paste Pak, Meron, Ultra Band Lok, and 3M Unitek Multi Cure) in each group. Genotoxic effects of the banding procedure for a 1-month period were evaluated using the micronucleus test (MNT). To monitor cytotoxic effects, binucleated cells (BNs), karyolysis (KL), and karyorrhexis (KR) were also evaluated in this setting. Results: Analysis of micronuclei in buccal epithelial cells revealed a significant increase in chromosomal damage in all groups (P < 0.01). Significant differences were found in the number of BNs in the groups receiving treatment with Meron, Ultra Band Lok, Durelon, and 3M Multi Cure (P < 0.01). Banding with GC Fuji and Durelon significantly elevated KL frequencies (P <0.05). Durelon had a significant effect on KR (P < 0.01). Conclusion: Band cementation with conventional glass ionomer cement (Meron) showed the least genotoxic effects. The highest cytotoxic effects on human oral buccal epithelium cells were found in the polycarboxylate cement (Durelon) group.
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    Microcomputed tomography evaluation of white spot lesion remineralization with various procedures
    (Mosby-Elsevier, 2016) Kucuk, Eyup Burak; Malkoc, Siddik; Demir, Abdullah
    Introduction: The aim of this study was to use microcomputed tomography to evaluate the effects on white spot lesions of 3 remineralizing agents compared with artificial saliva (Inonu University, Malatya, Turkey). The agents were GC Tooth Mousse (GC International, Itabashi-ku, Tokyo, Japan), 50-ppm sodium fluoride solution (Inonu University, Malatya, Turkey), and Clinpro 5000 (3M ESPE Dental Products (St Paul, Minn). The experimental and control teeth were stored in artificial saliva. Methods: Forty-four extracted premolars were divided into 4 groups of 11 teeth each (3 experimental groups and 1 control group). After white spot lesions were created on the teeth, a remineralizing agent was applied. Microcomputed tomography scanning was performed at the following times: T0 (sound enamel), T1 (day 0, when the white spot lesion was formed), T2 (day 15), and T3 (day 30). Volume, depth, surface area, and mineral density changes of the white spot lesions were evaluated at different time points using CTAn software (SkyScan; Bruker, Kontich, Belgium). Results: GC Tooth Mousse and Clinpro 5000 improved all measurements after 30 days. However, Clinpro 5000 was not as effective in reducing lesion depth as it was in the other parameters. The artificial saliva group and the 50-ppm sodium fluoride solution did not show significant effects in the regression of the white spot lesions at the end of the 30-day experiment. Conclusions: GC Tooth Mousse and Clinpro 5000 were more effective in remineralization of white spot lesions than sodium fluoride solution and artificial saliva. They can be preferred for use clinically. Microcomputed tomography is a novel and effective method that shows promise in accurately evaluating white spot lesions and remineralization.
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    Unusual bifid crista galli in a patient with cleft lip and palate
    (Springer, 2017) Dedeoglu, Numan; Altun, Oguzhan; Kucuk, Eyup Burak; Altindis, Sedat; Hatunoglu, Erdem
    One of the most common craniofacial malformations observed in newborn babies is cleft lip and palate (CLP). This syndrome presents with some anatomic variations. The aim of this study was to understand an anatomic variation of the crista galli that had not been reported previously in patients with CLP. A 17-year-old boy with CLP was referred to an orthodontic clinic for cone-beam computed tomography (CBCT) imaging before treatment. Axial and coronal sections and three-dimensional images showed that the crista galli had bifid heads that were attached to each other. Incomplete ossification had resulted in a bifid crista galli and the appearance of a suture in the anterior coronal section of the CBCT images. This anatomic variation could allow penetration of the anterior fossa during endoscopic surgery, which could be dangerous for the patient.