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Öğe The evaluation of pre-grown mycelial pellets in decolorization of textile dyes during repeated batch process(Springer, 2010) Yesilada, Ozfer; Yildirim, Seval Cing; Birhanli, Emre; Apohan, Elif; Asma, Dilek; Kuru, FilizThis study was undertaken for the possibility of application of pre-grown pellets for biotechnological treatment of dyes and textile industry waste waters. Mycelial pellets of five different white rot fungi were tested for their dye decolorization activity. The pellets of Funalia trogii, Phanerochaete chrysosporium and Trametes versicolor were determined as the most effective ones. The decolorization ability of viable pellets was compared with the decolorization (adsorption) ability of dead pellets during repeated batch studies. Astrazon Black dye was decolorized effectively, about 90%, by viable pellets of all fungi during the first use. Viable F. trogii pellets were found as the most effective pellets. Upon pellet treatment not only a high decolorization but also reduced toxicity (antimicrobial activity) of the Astrazon Black dye was recorded. This type of decolorization activity with commercial or crude laccase was partially observed. Growing cells of F. trogii in batch system showed lower efficiency in color removal of mixed dyes compared to the pre-grown pellets in repeated batch system. The results in this study showed that mycelial pellets could effectively be used as an alternative to traditional physicochemical processes.Öğe Katı substrat fermentasyonu ile lakkaz üretimi(İnönü Üniversitesi, 2007) Kuru, Filizkatı substrat üzerinde mikroorganizmaların üretilmesidir. KSF, funguslarla enzim üretimi için uygundur. Çeşitli lignoselülozik atıklar, lakkaz gibi ligninolitik enzim üretimi için katı substrat fermentasyonunda kullanılmaktadırlar. Beyaz çürükçül fungusların üretmiş olduğu lakkaz, fenolik ve fenolik olmayan bileşikleri oksitleme yeteneklerinden dolayı tercih edilmektedir. Bu çalışmada, katı substrat üzerinde Funalia trogii ve Trametes versicolor ile lakkaz üretimi test edilmiştir. Zeytinyağı fabrikası atıksuyu (ZYFA), alkol fabrikası atıksuyu (vinas), peyniraltı suyu ve melas, katı substratı nemlendirmek için kullanılmıştır. Bakır, pepton, glukoz, maya özütü, ksilidin gibi çeşitli indükleyiciler, katı substrat fermentasyonunda lakkaz üretimini arttırmak için denenmiştir. Bu indükleyicilerden özellikle bakır ilavesi, lakkaz aktivitesini arttırmıştır ve bazı atıksuların kullanımı, lakkaz üretimini pozitif yönde etkilemiştir. Sonuçlar, katı substrat fermentasyonu ile yüksek seviyede lakkaz üretilebileceğini göstermektedir. Ucuz ve bol tarımsal artık kullanımı, bu tekniğin ekonomik olmasını sağlamaktadır ve atıksu kullanımı, kirlilik problemlerini çözmeye yardımcı olabilir. ANAHTAR KELİMELER: Katı substrat fermentasyonu, lakkaz, beyaz çürükçül fungus, Funalia trogii, Trametes versicolorÖğe REMOVAL OF INDIGO CARMINE FROM AN AQUEOUS SOLUTION BY FUNGUS PLEUROTUS OSTREATUS(Polish Acad Sciences, Inst Environmental Engineering, 2012) Kahraman, Sibel; Kuru, Filiz; Dogan, Demet; Yesilada, OzferThe role of fungi in the treatment of wastewater has been extensively researched. Many genera of fungi have been employed for the dye decolourization either in living or dead form. In this study, the removal of an acidic dye, Indigo Carmine (IC), from an aqueous solution by biosorption on dead fungus, Pleurotus ostreatus, was investigated. The effects of contact time, initial dye concentration, amount of dead biomass, agitation rate and initial pH on dye removal have been determined. Experimental results show that an increase in the amount of dead biomass positively affected the dye removal. The highest removal was obtained at 150-200 rpm. Slightly lower removing activities were found at lower agitation rates. The dye adsorption efficiency was not affected by pH except minor variation in the pH of 2-8. Color removal was observed to occur rapidly within 60 minutes. The removal of dye by dead biomass of ostreatus was clearly dependent on the initial dye concentration of the solution. Dye removal was reduced from 93% to 64% as concentration was increased from 50 to 500 mg/L Indigo Carmine. This study showed that it was possible to remove textile dyes by dead biomass of P ostreatus.