DSpace Arşivi :: by Yazar "Kurucay, Ali" değerine göre listeleniyor

Yazar "Kurucay, Ali" seçeneğine göre listele

Listeleniyor 1 - 20 / 20

Anticancer, antioxidant, DFT calculations, and docking studies of some new peptide-indole conjugates
(Acg Publications, 2024) Kucukbay, Hasan; Gonul, Zeynep; Kurucay, Ali; Ates, Burhan; Boulebd, Houssem; Kucukbay, F. Zehra
In this study, the structures of six new peptide-indole derivatives were elucidated through spectroscopic and analytical methods following their synthesis. In addition to their anticancer and antioxidant properties, density functional theory (DFT) calculations and docking studies were conducted for the compounds. According to the obtained results, compounds 1 and 3 were identified as the most active against the MCF-7 cell line, with IC50 values of 8.72 and 5.86 mu g/mL, respectively. Conversely, compounds 4 and 1 were found to be the most active against the A549 cell line, with IC50 values of 15.43 and 16.10 mu g/mL, respectively. When compared to standard antioxidants using both the DPPH and iron reduction power assays, the compounds did not exhibit significant antioxidant activity. The molecular geometry and electronic properties of the synthesized peptide-indole derivatives were investigated through theoretical calculations using the Density Functional Theory (DFT) method. Molecular docking studies were also conducted to investigate the binding modes of the synthesized compounds within the active sites of EGFR enzyme.
Characterization of the antioxidant activity, total phenolic content, enzyme inhibition, and anticancer properties of Achillea millefolium L. (yarrow)
(Taylor & Francis Inc, 2022) Karaaslan Ayhan, Nagihan; Karaaslan Tunc, Merve Goksin; Noma, Samir Abbas Ali; Kurucay, Ali; Ates, Burhan
The antioxidant activity, total phenolic content, enzyme inhibition, anticancer properties of Achillea millefolium L. (yarrow), which is generally consumed by humans as herbal tea, were investigated. Yarrow extracts were prepared with different extraction techniques and solvents and the optimum conditions were determined. When the antioxidant activity and total phenolic content results were evaluated, the best yield was obtained with ultrasound-assisted extraction for all solvents. Moreover, the yields from highest to lowest were methanol, water, and acetonitrile. The enzyme inhibition and anticancer of Achillea millefolium L. extracts using methanol were evaluated. The IC50 values for the inhibition of xanthine oxidase and acetylcholinesterase were 4.974 +/- 0.54 and 21.891 +/- 1.118 mu g/mL, respectively. The extract concentration was determined to be 23.85 mu g/mL for 50% reduction in growth inhibitory activity cell viability (IC50) against breast cancer (MCF-7).
Design of near-infrared light induced functionalized upconverting nanoparticles as support in enzyme immobilization: Enhanced biocatalyst activity and stability
(Elsevier, 2025) Ulu, Ahmet; Noma, Samir Abbas Ali; Kurucay, Ali; Topel, Seda Demirel; Asilturk, Meltem; Ates, Burhan
In this study, we hypothesized that the emission of upconverted nanoparticles (UCNP) can trigger PEG-L-ASNase (P-Lase) activity through Forster Resonance Energy Transfer under near-infrared (NIR) irradiation. To enhance stability and activity of P-Lase, it was immobilized on functionalized NaYF4:Yb3+, Er3+, Nd3+. Upon immobilization, the obtained NaYF4:Yb3+, Er3+, Nd3+/GPTMS-P-Lase exhibited excellent pH stability, thermal stability, metal ions or organic solvent tolerance, and storage stability. The relative activity of NaYF4:Yb3+, Er3+, Nd3+/ GPTMS-P-Lase had about 65 % after 20 cycles and maintained 68 % and 59 % at +4 and 25 degrees C, respectively, after 4 weeks. Furthermore, in vitro cytotoxicity and hemolysis tests confirmed that the synthesized UCNPs were biocompatible. Most importantly, the activity of P-Lase was enhanced >= 4-fold under suitable NIR irradiation. It is reasonable to believe that this investigation may supply a novel technique to trigger the catalytic efficiency of P-Lase and may have promising application in leukemia treatment.
Design, synthesis, characterization, and biological activities of novel Ag (I)-NHC complexes based on 1,3-dioxane ligand
(Elsevier Science Inc, 2024) Ulu, Oznur Dogan; Kurucay, Ali; Gumushan, Ilkay Yildirim; Ozdemir, Namik; Ates, Burhan; Ozdemir, Ismail
Herein, a series of new Ag(I)-NHC complexes containing 1,3-dioxane group were synthesized by the direct reaction of Ag2O and benzimidazolium salts in light-free conditions. All Ag(I)-NHC complexes were spectrally characterized using 1H, 13C NMR, FT-IR, LC-MS, and elemental analysis. Additionally, the structures of compounds 1a and 1e were elucidated by the single X-ray diffraction techniques. Further, the synthesized Ag(I)-NHC complexes were evaluated for cytotoxicity study on the L-929 cells and the anticancer activity against the HCT 116 and MCF-7 cancer cell lines. Notably, 1a showed significant anticancer activity against HCT 116 with an IC50 of 6.37 f 0.92 mu g/mL compared to cisplatin (IC50 = 36.75 f 1.76 mu g/mL). 1c (IC50 = 3.21 f 1.96 mu g/mL) and 1e (IC50 = 3.72 f 1.12 mu g/mL) exhibited significant anticancer activity against MCF-7 cells and was similar to cisplatin (IC50 = 32.17 f 2.85 mu g/mL). Meanwhile, 1a and 1e displayed the highest selectivity index. Most importantly, the cell viability test showed that 1e induced neglectable cytotoxicity (IC50 = 36.38 f 2.27 mu g/mL) toward L-929 and was similar to cisplatin (IC50 = 36.11 f 2.09 mu g/mL). The anticancer activities of Ag(I)-NHC complexes vary depending on the substituent group of the silver complex and the cell line type. Moreover, the inhibitory mechanism of 1e was not dependent on caspase-associated apoptosis initiated by the lysosomalmitochondrial pathway. Taken together, we conclude that this work provides a simple and rapid protocol for the synthesis of Ag(I)-NHC complexes and the featured Ag(I)-NHC complexes have an anticancer drug potential for biomedical applications.
Development of 1,2,3-Triazolopyridazinone Derivatives as Potential Caspase 3 and Apoptosis Inducers: Design, Synthesis and Anticancer Activity Studies
(Wiley, 2025) Erdemir, Guler Yagiz; Kurucay, Ali; Ates, Burhan; Altundas, Aliye
Herein, the synthesis, anticancer activity and apoptotic pathways of 1,2,3-triazolopyridazinones compounds, which are similar to DNA bases not previously found in the literature have been investigated. To achieve this goal, it is designed the hybrid molecules combining triazole and pyridazinone/pyridazithione structures, bearing a lipophilic group (benzyl/phenyl) at the one position and benzene with electron withdrawing or donating groups at five positions, with high pharmacophoric properties on the same scaffold structure. The representative compounds in this series 5a, 5c, 6a and 8c exhibited higher anticancer activity than other compounds and cisplatin control against breast (MCF-7) and lung (A549) cell lines. These compounds were less toxic when tested against the noncancerous L929 cell line. In addition, the apoptotic effect mechanisms of these compounds were confirmed by AO/EB staining and caspase 3 activity results. These findings indicate that some derivatives of these compounds could be effective therapeutic agents for the treatment of cancer disease with an apoptosis-promoting.
The effects of Zn and Yb co-dopants on the electronic, radiation shielding, structural, thermal and spectroscopic properties of hydroxyapatite
(Elsevier, 2024) Ayhan, Yasemin Merve; Ates, Tankut; Seckin, Turgay; Ozcan, Imren; Bulut, Niyazi; Kurucay, Ali; Kaygili, Omer
This work presents a comprehensive investigation of the electronic properties of hydroxyapatite (HA) doped with zinc (Zn) and ytterbium (Yb). Four different compositions, namely 0.33Zn-0.33Yb-HA, 0.33Zn-0.66Yb-HA, 0.66Zn-0.33Yb-HA, and 0.66Zn-0.66Yb-HA, were studied using Density of States (DOS) and band structure calculations. The computed band gap values for each composition were determined to be 4.3097 eV, 4.1324 eV, 4.2527 eV, and 4.2088 eV, respectively. The observed decrease in the band gap energy from 0.33Zn-0.33Yb-HA to 0.66Zn-0.66Yb-HA signifies a significant impact of the dopant composition on the electronic properties of the material. Furthermore, the inclusion of ytterbium in the HA matrix resulted in the formation of a distinct band and peak in the density of states. This indicates the emergence of specific energy levels associated with Yb, suggesting a distinct influence on the electronic structure of the material. These findings provide valuable insights into the tunability of the electronic properties of HA through controlled doping with Zn and Yb. Such knowledge is crucial for tailoring materials with desired electronic characteristics, thus holding promise for various applications in electronic devices and biocompatible coatings. The as-modeled structures were synthesized via a wet chemical route. Fourier transform infrared (FTIR), Raman, and X-ray diffraction (XRD) analyses verified the formation of the HA structure for each sample. Differential thermal analysis (DTA) results showed that all the as-prepared samples were thermally stable. The negligible mass losses were detected for all the samples in the thermogravimetric analysis (TGA) measurements. The addition of both co-dopants affected crystal structure related parameters and decreased the crystallinity and cell viability.
Experimental and theoretical characterization of Bi-based hydroxyapatites doped with Ce
(Elsevier Sci Ltd, 2022) Kareem, Rebaz Obaid; Kaygili, Omer; Ates, Tankut; Bulut, Niyazi; Koytepe, Suleyman; Kurucay, Ali; Ercan, Filiz
This study deals with the theoretical and experimental characterizations of Bi-based hydroxyapatites (HAps) codoped with Ce. Five samples of Bi-based HAp (at a constant amount of 0.125 at.%) with additions of the Ce in various amounts (0, 0.125, 0.25, 0.375, and 0.500 at. %) were synthesized by using the wet chemical method. The prepared samples were investigated experimentally by X-ray diffraction (XRD), scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy, Raman spectroscopy, differential thermal analysis (DTA), thermogravimetric analysis (TGA), and vibrating sample magnetometer (VSM). All the samples were also modeled by using a density functional theory (DFT), and theoretical results were obtained. Both experimental and theoretical results showed that the lattice parameters and unit cell volume were significantly affected by Ce content. Calculated bandgap energy results of the samples gradually reduced from 4.6308 eV to 4.5299 eV. The bandgap decreased with increasing Ce content, and the densities of states (DOS) values were also affected by the amount of Ce. It was found that the sample doped with 0.500 at. % Ce showed the best biocompatibility among all the as-synthesized samples. The linear absorption coefficient increased with increasing amounts of Ce in all samples, while this parameter decreased with increasing photon energy. The density increases with the increasing Ce content ranging from 3.1615 g cm-3 to 3.1772 g cm- 3. Both crystallite size and crystallinity decreased gradually with the increasing amount of Ce. FTIR and Raman spectra confirm the formation of the HAp structure for all the samples.
The experimental and theoretical investigation of Sm/Mg co-doped hydroxyapatites
(Elsevier, 2022) Hssain, Ala Hamd; Bulut, Niyazi; Ates, Tankut; Koytepe, Suleyman; Kurucay, Ali; Kebiroglu, Hanifi; Kaygili, Omer
This study presents an extensive experimental and theoretical analysis of Mg -doped hydroxyapatite (HAp) samples with additional Sm doping at varying amounts. A wet chemical method was used to synthesize Mg-containing HAps at a constant amount of 0.133 at. %, and in addition the second dopant of Sm was utilized at varying amounts of 0.133, 0.266, 0.399, 0.532, and 0.665 at. %. All of these samples were investigated experimentally and theoretically by using the X-ray diffraction (XRD) analysis, Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), Raman spectroscopy, differential thermal analysis (DTA), thermogravimetric analysis (TGA), in-vitro cell viability tests, and density functional theory (DFT) calculations. The bandgap energy was found to have reduced from 4.6115 eV to 4.3870 eV. The linear attenuation (or absorption) coefficient increased as the amount of Sm in the sample increased, and this parameter decreased as the photon energy increased for all samples. Both the lattice parameter and the theoretical density increased, whereas the unit cell volume and the lattice parameters declined. The XRD and FTIR results revealed that the HAp phase (over 98 percent for all samples) was a major phase formed, while the beta -TCP phase (beta-tricalcium phosphate) was a minor phase. The beta-TCP phase increased from 0.91 to 1.57 percent with an increasing amount of Sm. The volume of the unit cell trended in the same direction as predicted by the obtained results theoretically. Increased anisotropic energy density and a decrease in crystallinity have been observed. All samples were found to be thermally stable. Due to the presence of Sm content, cell viability was observed to be affected.
Experimental characterization and theoretical investigation of Zn/Sm co-doped hydroxyapatites
(Elsevier, 2022) Hssain, Ala Hamd; Bulut, Niyazi; Ates, Tankut; Koytepe, Suleyman; Kurucay, Ali; Kebiroglu, Hanifi; Kaygili, Omer
In this study, the wet chemical method was used to synthesize Zn-doped hydroxyapatite (HAp) samples, and the effects of varying the amount of Sm addition on structural, thermal, and biocompatibility in vitro properties were studied. In addition, a density functional theory was used for modeling the as-synthesized samples to obtain the theoretical calculation results. XRD results confirmed the formation of biphasic compositions for all samples, and FTIR data supported the formation of the functional groups of hydroxyl and phosphate. More than 98% of samples showed the formation of the HAp phase. The addition of Sm resulted in an increase in the secondary phase of the beta-TCP from 0.60 % to 1.49 %. The lattice parameters (aandc), unit cell volume (V), lattice strain (epsilon), and lattice stress (sigma) varied when Sm was added as a dopant. The crystallite size and crystallinity decreased as the Sm content increased, however, the anisotropic energy density gradually increased. Thermal analysis results confirmed that all samples seemed to be thermally stable. The addition of Sm did not result in any notable morphological modifications. Cell viability values of the Zn-based HAp sharply decreased as a result of an increase in the Sm additive. Theoretical studies show that when the amount of Sm in the Zn-based HAp structure increases, the bandgap energy decreases from 4.68 to 4.40eV. An increasing density and decreasing unit cell volume have been observed, as confirmed by the theoretical results. In addition, there was a decrease in crystallinity as well as an increase in anisotropic energy density.
Increasing the Functionality of Surgical Masks with Water-Soluble Antibacterial Polymers and MTT Test Studies
(Springernature, 2024) Oktay, Aysenur Cakmak; Demirci, Sevilay; Tanriverdi, Aslihan Aycan; Kurucay, Ali; Ata, Ahmet Cagri
Herein, reversible addition-fragmentation chain transfer (RAFT) agent salts containing water-soluble, antibacterial macro xanthate polyethylene glycol (PEG) were synthesized. Macro xanthate RAFT agent salts were featured by proton nuclear magnetic resonance (1H-NMR) spectroscopy and Fourier transform infrared (FT-IR) spectroscopy. PEG1500 Macro Xanthate RAFT agent pure water and PEG400 Macro Xanthate RAFT pure water solutions were applied to surgical masks by spraying. Moreover, these polymers were used to determine the minimum inhibitory concentration (MIC) on standard bacterial strains of Gram-positive bacteria and Gram-negative bacteria in the modified Broth MicroDilution method. It was determined that the synthesized polymers prevented the growth of bacteria. Mus musculus mouse fibroblast cells (L-929) were used in the cytotoxicity test of the materials performed by the direct method. It was determined by the Cytotoxicity 3-4,5-dimethyl-thiazolyl-2,5-diphenyltetrazolium bromide (MTT) test that these polymers were not harmful to human health. The biocompatibility of Macro xanthate RAFT agent salts was above 80%.
Nature-derived otolith loaded polyethylene glycol-polycaprolactone electrospun composite nanofiber membranes: preparation, characterization, and biocompatibility evaluation
(Springer, 2025) Pekdemir, Sibel Selcuk; Gedik, Kubra Karadas; Kurucay, Ali; Onay, Hatice; Ulu, Ahmet; Pekdemir, Mustafa Ersin; Kolak, Seda
The electrospun nanofibers have paid much attention to biomedical applications. In this study, the electrospun composite nanofibers were prepared based on optimized polycaprolactone (PCL, 12 wt%) and polyethylene glycol (PEG, 3.6 wt%) polymers loaded with otoliths particles (OTO, 10-30 wt%) by electrospinning technique. The morphological, molecular interactions, crystallinity, and thermal properties of the composite nanofiber membranes were characterized by scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, thermogravimetric analysis, and differential scanning calorimetry. In addition to that, swelling behaviors, biodegradability, porosity, and biocompatibility were also evaluated. Both PEG/PCL and OTO/PEG/PCL nanofibers revealed bead-less constructions with average diameters of around 500 nm. Thermogravimetric analysis revealed OTO/PEG/PCL composite membranes thermally stable up to 300 degrees C. XRD results also indicated a good crystallinity for the OTO/PEG/PCL composite membranes due to higher crystallinity of otolith. Adding otolith to PEG/PCL nanofibers did not obviously change the water uptake capacity, biodegradability, and porosity while increasing swelling ratio. Finally, membranes with the lowest otolith concentration (10% w/w) showed 96.2% cell viability, while increasing otolith concentration decreased cell viability. Based on the obtained results, the cytotoxicity of OTO/PEG/PCL membranes was evaluated with mouse fibroblast (L-929) cells by more than 85% survival during 72 h, which revealed that the OTO/PEG/PCL membranes were non-toxic. Taken together, these data suggest that OTO/PEG/PCL membranes could potentially be used as a nanofiber scaffold for applications.
Near-infrared inducible supports in bio-catalysts design: A useful and versatile tool in enhancement of enzyme activity
(Elsevier, 2024) Noma, Samir Abbas Ali; Dik, Gamze; Gurses, Canbolat; Kurucay, Ali; Topel, Seda Demirel; Ulu, Ahmet; Asiltuerk, Meltem
Immobilized enzymes have encountered two main challenges: Reduced enzyme activity compared to free enzymes and exhausted immobilized enzymes due to reusability. Herein, we suggested a promising activity enhancement strategy to overcome these challenges. The emission from upconversion nanoparticles (UCNPs) under near-infrared (NIR) excitation can increase the activity of PEG-L-ASNase due to Forster Resonance Energy Transfer. For this purpose, UCNPs were initially synthesized using the hydrothermal method. Subsequently, these UCNPs were functionalized with a polycationic polymer, branched polyethyleneimine (PEI), and the immobilization of PEG-L-ASNase was achieved through adsorption. We preliminarily explored the parameters such as enzyme concentration, incubation time, pH, temperature, reusability, storage stability, and kinetic study, etc. Further, the in vitro biocompatibility, hemolytic behavior, and anticancer activity of the produced UCNPs were also analyzed as crucial parameters. The results showed the pH durance, thermal and storage stability of the immobilized PEG-L-ASNases were enhanced. The immobilized PEG-L-ASNases maintained their activity to >= 55 % after 20 cycles. Enzyme immobilization led to a decrease in Km and Vmax compared to PEG-L-ASNase. In vitro assays revealed that immobilized enzyme further reduced the proliferation of human leukemia cell line (HL-60) upon NIR irradiation exposure but did not cause toxicity. This research may provide a new strategy to promote the catalytic activity of L-ASNase and demonstrates its potential application on human leukemia cells. Finally, these outcomes are valuable for the use of NIR induction in enzymatic reactions.
New morpholine or triphenylphosphine-liganded palladium(II) N-heterocyclic carbene complexes: Synthesis, characterization, crystal structure, and anticancer activity
(Elsevier Science Sa, 2026) Gok, Yetkin; Ozkan, Gul; Kurucay, Ali; Ates, Burhan; Aktas, Aydin; Demirci, Ozlem; Aygun, Muhittin
A series of morpholine(Morp.)-liganded palladium(II) complexes (1a-c) and triphenylphosphine(PPh3)-liganded palladium(II) complexes (2a-c) bearing 4-fluorobenzyl substituted N-heterocyclic carbene (NHC) were synthesized from NHC-Pd(II)-pyridine and Morp./PPh3 by ligand exchange method. The new complexes were fully characterized using 1H NMR, 13C NMR, 19F NMR, 31P NMR, FTIR spectroscopy and elemental analysis techniques. Furthermore, single crystal X-ray diffraction was used to elucidate the structures of complexes 1a and 2b. The anticancer activities of the new complexes against MCF-7 (Human Breast Cancer) cell line were investigated. With an IC50 value of 37.54 for complex 1a, it can be said that it is more cytotoxic to MCF-7 cells compared to other complexes, while the least cytotoxicity was observed in complex 1b.
pH-responsive magnetic cyclodextrin-liposome nanoplatforms for stabilization and targeted delivery of topotecan
(Elsevier, 2026) Tural, Bilsen; Ertas, Erdal; Kurucay, Ali; Ates, Burhan; Tural, Servet
Topotecan (TPT), a potent anticancer agent, suffers from rapid hydrolysis of its active lactone ring under physiological conditions, severely limiting its clinical efficacy. To address this challenge, we developed a novel pH-responsive, magnetic drug delivery system integrating (3-cyclodextrin-functionalized Fe3O4 nanoparticles (Fe3O4@(3-CD) and liposomes. Fe3O4@(3-CD nanoparticles were synthesized via chemical co-precipitation and then loaded with TPT via host-guest complexation. The resulting Fe3O4@(3-CD@TPT complexes were encapsulated into liposomes using the sonication method to increase drug stability, maintain the stable lactone form, and achieve controlled release. Characterization by Transmission Electron Microscopy (TEM), Dynamic Light Scattering (DLS), Fourier Transform Infrared Spectroscopy (FTIR), High-Performance Liquid Chromatography (HPLC), and Vibrating Sample Magnetometry (VSM) confirmed the successful formulation. In vitro drug release studies demonstrated a diffusion-controlled release profile, while the pH responsiveness of the system was primarily governed by pH-dependent drug stabilization and loading efficiency rather than pronounced differences in release kinetics. Cytotoxicity assays against MCF-7 breast cancer cells showed that the Fe3O4@(3CD@TPT@Liposome formulation exhibited significantly lower IC50 values compared to free TPT and Fe3O4@(3CD@TPT complexes. These results highlight the potential of the magnetic cyclodextrin-liposome hybrid platform as an effective strategy for the targeted and controlled delivery of pH-sensitive chemotherapeutics such as topotecan.
Ru(II)-NHC complexes bearing benzhydryl ligand: Potent anticancer agents and highly efficient catalysts for transfer hydrogenation
(Pergamon-Elsevier Science Ltd, 2026) Ulu, Oznurr Dogan; Kurucay, Ali; Ulu, Ahmet; Ates, Burhan; Ozdemir, Ismail
Ruthenium(II) N-heterocyclic carbene (NHC) complexes are widely studied due to their promising anticancer activities and catalytic properties. Herein, five novel Ru(II)-NHC complexes were synthesized and characterized using various methods such as elemental analysis, FT-IR, 1H NMR, and 13C NMR techniques. The catalytic activities of the complexes in the transfer hydrogenation reaction were tested and the results showed that they were effective catalysts in this reaction. Moreover, in vitro anticancer activities were investigated based on three cell lines, including two cancer cell lines (HCT 116, A549) and one normal cell line (L-929). All complexes exhibited significant cytotoxic activity at all tested concentrations against the cancer cell lines. The IC50 values showed that the complexes exhibited superior activity as compared to cisplatin. The anticancer activities of Ru-NHC complexes vary depending on the substituent group of the complex and the type of cell line. However, complex 2a showed better anticancer activity (IC50 = 7.66 f 2.83 mu M) than that exhibited by cisplatin (IC50 = 121.78 f 9.35 mu M) against the HCT116 cell line. Similarly, complex 2a (IC50 = 42.47 f 3.10 mu M) demonstrated significant activity compared to cisplatin (IC50 = 181.23 f 5.44 mu M) against the A549 cell line. In contrast, complexes exhibited low cytotoxic activity against L-929 cells (33.65-95.18 mu M). Moreover, cell imaging of complexes 2a, 2c, and 2d was examined against cell lines using fluorescence microscopy. Based on our results, complex 2a is an intriguing candidate for future investigations as a potential anticancer drug for the treatment of different cancers.
Silver and selenium compounds of benzimidazolium salts containing fluorine substituents: Synthesis, characterization, and evaluation of anticancer potentials
(Elsevier Science Sa, 2025) Karakas, Gulsen; Serin, Suemeyya; Mumcu, Akin; Ulu, Oznur Dogan; Ulu, Ahmet; Kurucay, Ali; Ates, Burhan
The applications of fluorine in the field of drug design are expanding at a rapid pace. The development of synthetic methodologies has enabled the creation of new fluorinated motifs. Benzimidazolium salts represent a significant source of N-heterocyclic carbenes (NHCs), a distinctive class of ligands. These salts have a wide range of applications due to their notable qualities. The present study encompasses the synthesis of four novel benzimidazolium salts (1a-d) substituted with 2,6-difluorobenzyl moiety. Subsequently, the prepared salts were utilized in the synthesis of silver complexes (2a-d) and selenium compounds (3a-d). The structures of all compounds were elucidated by 1H, 13C, 19F NMR, HRMS, FT-IR spectroscopies, and elemental analysis techniques. The incorporation of a fluorine atom into the molecular structure of a compound has been known to induce substantial alterations in the biological responses of mentioned compound. Therefore, the cytotoxic effect of these compounds was also evaluated using the MTT assay on HCT116 and L929 cell lines. Acridine orange (AO)/ethidium bromide (EB) staining were used for the detection of apoptosis. Notably, cytotoxicity assays showed that compound 3d displayed approximately 22-fold higher potency against HCT116 than cisplatin. Taken together, this enhanced efficacy signifies the promising role of silver and selenium compounds of benzimidazolium salts containing fluorine substituents in anticancer therapies.
Sr/Smco-doped hydroxyapatites: experimental characterization and theoretical research
(Springer, 2022) Hssain, Ala Hamd; Bulut, Niyazi; Ates, Tankut; Koytepe, Suleyman; Kurucay, Ali; Kebiroglu, Hanifi; Kaygili, Omer
This paper goes into extensive detail about the theoretical and experimental characterization of Sr doped hydroxyapatite (HAp) samples that have been doped with Sm in various amounts. To accomplish this, five HAps containing a constant 0.133at.% of Sr were additionally doped with Sm at varied amounts and synthesized by a method of wet chemical. In addition, the density functional theory (DFT) was used to model all of these samples. According to theoretical results, the bandgap energy declined continuously from 4.6297 to 4.4034eV. The linear absorption coefficient increased with increasing amounts of Sm in all samples, while this parameter decreased with increasing photon energy. There was a reduction in both the lattice parameter a and the volume of the unit cell, but there was also an increase in the lattice parameter c and theoretical density. Accordingly, the experiment's results were as follows:HAp phase (above 98% for all of the samples) and beta-tricalcium phosphate beta - TCP were both confirmed as the major phase and minor phase, respectively, by X-ray diffraction (XRD), FT-Raman spectroscopy and the Fourier transform infrared (FTIR) measurements. The addition of Sm increased in the beta - TCP phase from 0.62 to 0.86%. Same as theoretical results, it was also found that density was increasing and the volume of the unit cell decreased. An increase in anisotropic energy density and a decrease in crystallinity were discovered. The values are calculated based on the molar ratio, which was discovered to be close to those for stoichiometric HAp in all samples (1.67). Based on the results of the thermal study, all samples were confirmed to be thermally stable. Sm content was discovered to have an effect on cell viability.
Structural, magnetic, thermal, biocompatibility, and electronic properties of lanthanum doped-magnesium hydroxyapatite
(Elsevier Sci Ltd, 2025) Mahmood, Bahroz Kareem; Kareem, Rebaz Obaid; Bulut, Niyazi; Ates, Tankut; Keser, Serhat; Kaygili, Omer; Kurucay, Ali
This study presents the preliminary experimental and theoretical results on Mg/La co-substituted in hydroxyapatite (HAp) structure. Four Mg-based HAps were synthesized with La doping by keeping the Mg concentration constant at 0.45 at.%, while the La content was varied from 0.45 to 1.80 at.% in 0.45 at.% increments by a wet chemical method. Experimentally, the major phase for all samples was HAp and the minor phase was beta-TCP. The presence of La as a dopant for the HAp structure was found to affect the lattice parameters. The values of lattice parameters and unit cell volume were observed to increase gradually. The crystallinity percentage ranged from 85 % to 89 %. Analysis using the Scherrer and Williamson-Hall approaches revealed that the crystallite size values of the samples were in the range of 22-29 nm and 23-33 nm respectively. In addition, the values of lattice stress, strain and anisotropic energy density were influenced by the concentration of La. The magnetic saturation decreased from 0.0439 to 0.0383 emu/g with an increase in the amount of La. All samples showed biocompatible properties. A slight change in morphology was also observed. The EDX results showed that the presence of La at different levels resulted in calcium deficiency. Thermogravimetric analysis and differential thermal analysis, carried out over a temperature range of 25-850 degrees C, showed that each sample had thermal stability, with no exothermic or endothermic peaks detected. Theoretical results derived from Density Functional Theory (DFT) calculations showed that the band gap values decreased steadily from 4.578 to 4.438 eV.
Synthesis, characterization, in vitro antibacterial, and anticancer studies of Ag(I)-N-heterocyclic carbene (NHC) complexes
(Springer Int Publ Ag, 2023) Ulu, Oznur Dogan; Kurucay, Ali; Ates, Burhan; Ozdemir, Ismail
N-Heterocyclic carbene (NHC) complexes of metals such as Ag(I), Au(I), Pd(II), and Cu(I) are very interesting compounds due to both their catalytic and biological properties. In particular, Ag(I)-NHC complexes show activity as antibacterial, antimicrobial, and anticancer agents. Therefore, in this study, six new Ag(I) complexes were obtained from the reaction of benzimidazolium salts with Ag2O. The structures of the prepared compounds were elucidated by FT-IR, H-1 NMR, C-13 NMR, LC-MS spectroscopic methods, and elemental analysis. The antibacterial activity of all Ag(I)-NHC complexes was evaluated against bacterial strains E. coli as gram-negative and B. subtilis as gram-positive bacteria using ampicillin as a standard antibiotic. Moreover, all Ag(I)-NHC complexes were investigated for anticancer activity against the breast adenocarcinoma cell line (MCF-7). All complexes showed good activity against two bacterial strains, even complexes 2d and 2e had more bactericidal activity against B. subtilis than ampicillin. Moreover, complexes 2a (IC50 6.01 +/- 2.39 mu g/mL) and 2e (IC50 3.40 +/- 0.87 mu g/mL) exhibited a better anticancer effect against to MCF-7 cell line than cisplatin (IC50 82.02 +/- 6.19 mu g/mL). Finally, it is worthy to note that the biochemical outcomes revealed that these complexes may be promising antibacterial and anticancer agents.
Water-soluble N-heterocyclic carbene precursors bearing benzimidazole core: synthesis, characterization, in vitro antioxidant and anticancer studies
(Springer Wien, 2023) Ulu, Oznur Dogan; Kurucay, Ali; Ozdemir, Ismail
Benzimidazole is an important member of the nitrogen-containing heterocyclic ring system since it confers surprisingly good biological properties in medicinal chemistry such as anticancer drug applications. Keeping this in mind, a new series of asymmetrical 1,3-disubstituted benzimidazolium salts were designed and reported. The compounds were synthesized by the reaction of 1-alkylbenzimidazoles with 2-(2-bromoethyl)-1,3-dioxane. The structures of all the compounds were determined using FT-IR, H-1 and C-13 NMR spectroscopy, LC-MS, and elemental analysis. The compounds were used for their antioxidant capacity by commercial kit assay. Further, these were screened for their cytotoxic effect against cell lines including MCF-7, A-549, and L929 using cisplatin as a reference drug. It resulted that the total antioxidant capacity values of the compounds were found to be quite low compared to the trolox equivalent. On the other side, most importantly, two compounds showed remarkable anticancer activity against MCF-7 and A-549 with IC50 values of 18.06 and 20.44 mu g/cm(3), respectively, in comparison with cisplatin (IC50 = 83.11 and 87.80 mu g/cm(3)). Furthermore, most of the compounds were found to be safe on normal L929 fibroblast cells compared to cancer cells (IC50 values for healthy fibroblasts were 142.70-268.37 mu g/cm(3)). Taken together, the newly synthesized compounds containing benzimidazole core can be readily constructed and used as an attractive class of anticancer agents.{graphical abstracts}

Yazar "Kurucay, Ali" seçeneğine göre listele

Sayfa Başına Sonuç

Sıralama seçenekleri